3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo citations for BioLegend

Quote: ... 50-μm brain sections were blocked with normal goat serum for 2 h and incubated overnight using biotin-conjugated anti-HA (Biolegend 901505, 1:500) and anti-parvalbumin (Synaptic Systems 195 004 ...

Learning and Sleep Have Divergent Effects on Cytosolic and Membrane-Associated Ribosomal mRNA Profiles in Hippocampal Neurons

James Delorme, et al., bioRxiv - Neuroscience 2020

Quote: ... 50μM coronal sections containing dorsal hippocampus were blocked in normal goat serum for 2 h and incubated overnight using a biotin conjugated anti-HA (Biolegend 901505, 1:500), anti-pS6 244-247 (ThermoFisher 44-923G ...

Extracellular Vesicle-Mediated Purinergic Signaling Contributes to Host Microenvironment Plasticity and Metastasis in Triple Negative Breast Cancer

Suzann Duan, et al., bioRxiv - Cancer Biology 2020

Quote: ... coated with intercellular adhesion molecule (ICAM-1, ICAM-2) and vascular cell adhesion molecule (VCAM-1) antibodies conjugated to Alexa Fluor 680 (BioLegend, San Diego, CA). Sorted cells were cultured in Endothelial Growth Media (EGM-2 ...

FoxP3 can fold into two distinct dimerization states with divergent functional implications for T cell homeostasis

Fangwei Leng, et al., bioRxiv - Immunology 2022

Quote: ... extracellular staining was done in ice-cold buffer (phenol red–free DMEM, 2% FBS) for 30 min using antibodies against CD45 (30-F11; BioLegend, dilution 1:200), CD4 (RM4-5 ...

The Olfm4-defined human neutrophil subsets differ in proteomic profile in septic shock

Hans Lundquist, et al., bioRxiv - Immunology 2022

Quote: ... 2×107 cells resuspended in 0.5 ml PBS were incubated with PE-conjugated mouse anti-human CD15 antibody (BioLegend, San Diego, CA, USA) diluted 1:40 ...

Nasal prevention of SARS-CoV-2 infection by intranasal influenza-based boost vaccination

Runhong Zhou, et al., bioRxiv - Immunology 2021

Quote: ... cells were washed with staining buffer (PBS containing 2% FBS) and surface stained with anti-mouse-CD4-PerCP/Cy5.5 (Biolegend Cat# 116012, RRID: AB_2563023), anti-mouse-CD8-BV785 (Biolegend Cat# 100750 ...

A comparative analysis of SARS-CoV-2 antivirals in human airway models characterizes 3CL^pro inhibitor PF-00835231 as a potential new treatment for COVID-19

Maren de Vries, et al., bioRxiv - Microbiology 2021

Quote: ... approximately 200,000 cells per sample were resuspended in staining buffer (PBS, 2% BSA, 0.01% Tween) and incubated for 10 minutes with Fc block (TruStain FcX, Biolegend; FcR blocking reagent, Miltenyi). Cells were then incubated with barcoded hashing antibodies for 30 min at 4 °C ...

G307S DNAM-1 mutation exacerbates autoimmune encephalomyelitis via enhancing CD4⁺ T cell activation

Rikito Murata, et al., bioRxiv - Immunology 2022

Quote: ... Sorted naïve 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend, San Diego, CA) and soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...

Exploring heteroaromatic rings as a replacement for the labile amide of antiplasmodial pantothenamides

Jinming Guan, et al., bioRxiv - Pharmacology and Toxicology 2020

Quote: ... 1 μg/mL anti-CD71 APC (clone R17217) (eBioscience, San Diego, CA) and 2 μg/mL anti-TER119 PE-Cyanine7 (BioLegend, San Diego, CA). The cells were stained on ice for 30 min ...

Structural surfaceomics reveals an AML-specific conformation of Integrin-β2 as a CAR-T therapy target

Kamal Mandal, et al., bioRxiv - Cancer Biology 2022

Quote: CAR-T cells were co-cultured with tumor at ratio of 2:1 for 6 hours at 37o C in CO2 incubator with CD107a antibody (Biolegend, 328620, clone-H4A3) and golgistop (BD Biosciences ...

Apolipoprotein E2 Promotes Melanoma Growth, Metastasis, and Protein Synthesis via the LRP1 Receptor

Nneoma Adaku, et al., bioRxiv - Cancer Biology 2022

Quote: ... Cells were then resuspended in 50 µl of flow buffer (1x PBS, 2% FBS, 10mM EDTA, 25mM HEPES) containing anti-CD16/CD32 antibody for Fc receptor blockade (Biolegend, 101320, 1:200) and incubated on ice for 5 minutes ...

Development of a Robust Consensus Modeling Approach for Identifying Cellular and Media Metabolites Predictive of Mesenchymal Stromal Cell Potency

Alexandria Van Grouw, et al., bioRxiv - Bioengineering 2023

Quote: ... PBMCs were collected and stained using Brilliant Violet 711 anti-CD4 and APC anti-CD8 (Supporting Information Table 2) (Biolegend, San Diego CA). PBMCs were first washed and stained with Zombie Yellow (Supporting Information Table 2 ...

B cell-intrinsic STAT3-mediated support of latency and interferon suppression during murine gammaherpesvirus 68 infection revealed through an in vivo competition model

Chad H. Hogan, et al., bioRxiv - Microbiology 2023

Quote: ... 2 million splenocytes were resuspended in 50 µl of fluorescence-activated cell sorter (FACS) buffer (PBS with 2% fetal bovine serum) and blocked with TruStain fcX (clone 93; BioLegend, San Diego, CA). The cells were washed and stained to identify B cell subsets with fluorophore-conjugated antibodies against CD45.2 (clone 104) ...

Replication-dead gammaherpesvirus vaccine protects against acute replication, reactivation from latency, and lethal challenge in mice

Wesley A. Bland, et al., bioRxiv - Microbiology 2023

Quote: 2 × 106 single cell suspensions prepared from the lung or spleen were resuspended in 200 ul of PBS with 2% fetal bovine serum and blocked with TruStain fcX (BioLegend, San Diego, CA). T cell subsets were identified with antibodies against CD45 (clone 30-F11 ...

Targeting LxCxE cleft pocket of retinoblastoma protein in M2 macrophages inhibits ovarian cancer progression

Evgenii N. Tcyganov, et al., bioRxiv - Immunology 2024

Quote: ... Splenocytes and ascitic cells were isolated and re-stimulated with SIINFEKL peptide (immunodominant ovalbumin peptide) for 12 hours in the presence of 2 uM Monensin (BioLegend, San Diego, CA) with the following intracellular staining of IFN-γ and measurement of IFN-γ-producing CD8+ T cells (meaning ovalbumin-specific T cells ...

Vaccination with a Replication-Dead Murine Gammaherpesvirus Lacking Viral Pathogenesis Genes Inhibits WT Virus Infection

Dipanwita Mitra, et al., bioRxiv - Microbiology 2024

Quote: ... 2 X 106 single cell suspensions prepared from the lung or spleen were resuspended in 200 μl of PBS with 2% fetal bovine serum and blocked with TruStain fcX (BioLegend, San Diego, CA). T cell subsets were identified with antibodies against CD45 (dilution 1:200 ...

Drug-controlled CAR-T cells through the regulation of cell-cell interactions

Leo Scheller, et al., bioRxiv - Synthetic Biology 2024

Quote: ... supernatant samples were collected to measure IFN-γ and IL-2 using commercial enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer’s instructions (BioLegend, 430801 and 431801).

A Commander-independent function of COMMD3 in endosomal trafficking

Galen T. Squiers, et al., bioRxiv - Cell Biology 2024

Quote: ... the cells were labeled for one hour with KRH buffer containing 2% FBE and the following antibodies: anti-HA (BioLegend, #901501, RRID: AB_2565006), APC-conjugated anti-LAMP1 (BioLegend ...

Macrophages in the uterus are functionally specialised and continually replenished from the circulation

Nicholas A. Scott, et al., bioRxiv - Immunology 2021

Quote: ... Recombinant murine IL-4 (Biolegend) was combined with rat IgG1 mAb to IL-4 ...

Protein-based RBD-C-tag COVID-19 Vaccination Candidate Elicits Protection Activity against SARS-COV-2 Variant Infection

Jaganathan Subramani, et al., bioRxiv - Microbiology 2021

Quote: ... IL-4 APC (Biolegend, #504106), IL-17 PECy7 (Biolegend ...

Mapping the immunogenic landscape of near-native HIV-1 envelope trimers in non-human primates

Christopher A. Cottrell, et al., bioRxiv - Immunology 2020

Quote: ... CD4 ([clone OKT-4] BioLegend), CD3 ([clone SP34-2] BD) ...

Public and private human T cell clones respond differentially to HCMV antigen when boosted by CD3 co-potentiation

Laura R.E. Becher, et al., bioRxiv - Immunology 2020

Quote: ... anti-Vβ5 (BioLegend, MR9-4), and anti-Nur77 (Invitrogen ...