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Citations for BioLegend :
951 - 1000 of 2017 citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Cells were cultured in a 96-well round bottom plate at a density of 50,000-600,000 cells/300 µL with 100 U/mL IL-2 (Biolegend, Cat. 575404 (mouse) 589104 (human)) ...
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bioRxiv - Immunology 2024Quote: ... labeled RMA-S cells at a 2:1 Effector to Target ratio in RPMI supplemented with 10ng/mL rIL-15 (Biolegend 566302). Co-cultures were incubated at 37° degrees for 5 hours ...
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bioRxiv - Immunology 2024Quote: ... and one of the hashtag antibodies (TotalSeq™-C0251 anti-human Hashtag 1 Antibody, LNH-94 2M2, BioLegend #394661; TotalSeq™-C0252 anti-human Hashtag 2 Antibody, LNH-94 2M2, BioLegend #394663 ...
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bioRxiv - Bioengineering 2024Quote: ... supernatants were drawn off the cells and analyzed for cytokine content (i.e., IL-2, IFN-γ, and TNF-α) by ELISA kits (Biolegend).
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bioRxiv - Immunology 2024Quote: ... single cell suspension was incubated with the cell hashtag antibodies (unique DNA barcodes listed in Supplementary Table 2) and the cocktail of 119 unique cell surface antibodies (Biolegend, #199901), as per manufacturer’s protocol.
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bioRxiv - Neuroscience 2024Quote: ... The cells were re-suspended in PBS / 2% HI-FBS / 10mM EDTA-Na (pH 8.0) and stained with PE/Cyanine7 anti-mouse CD45 (BioLegend, #103114, RRID:AB_312979) and FITC anti-mouse/human CD11b (BioLegend ...
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bioRxiv - Neuroscience 2024Quote: ... We then blocked the brains in 5 % goat serum for 2 h and stained with mouse anti-Aβ42 (6E10) (1:500, BioLegend, CA, USA) in PBST on a shaker at 4 °C overnight ...
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bioRxiv - Immunology 2024Quote: ... for 20 minutes at 37°C and resuspended in cRPMI supplemented with 5 ng/mL recombinant mouse IL-2 (Biolegend #575404). 5×105 cells/well plated in a 96-well round-bottom plate ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated for 2 h with Alexa Fluor® 647 anti-Tubulin-α antibody (1:250 dilution, clone 10D8, BioLegend) prepared in the permeabilization/blocking buffer ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were treated with mechanosensing and transduction inhibitors using an antibody against integrin β1 (2 µg/mL; anti-integrin β1; Biolegend, USA) and chemical inhibitors against PI3K (1 µM ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed twice with 2 mL of PBS + BSA (400 g for 5 min) and run fixed (Biolegend fixation buffer) or unfixed prior to sorting and analysis ...
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bioRxiv - Cell Biology 2024Quote: ... were thawed in a 37°C water bath for 2 mins and resuspended in 10 ml of warm Staining Buffer (SB; Biolegend #420201). Cells were washed twice at 300 g for 10 mins ...
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bioRxiv - Cell Biology 2024Quote: ... were thawed in a 37°C water bath for 2 mins and resuspended in 10 ml of warm staining buffer (SB; Biolegend #420201) or media buffer (Thermo Fisher Scientific DMEM #12634010 or RPMI #61870010 ...
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bioRxiv - Developmental Biology 2024Quote: ... Cell pellets were collected by centrifugation and resuspended in 100µl PBS/2% serum supplemented with Alexa Fluor 647 anti-CD15 (SSEA-1) (Biolegend, cat. 125608) and Phycoerythrin (PE ...
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bioRxiv - Immunology 2022Quote: ... Tim-3 was either neutralized by anti-human Tim-3 antibodies (10 µg/mL) (Biolegend) for 1 h prior to treatment or through knockdown via duplexed siRNA (Integrated DNA Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated for 30 minutes at 4°C with pre-chilled 4°C PBS (200 uL) containing 4 uL FITC-conjugated anti-CD63 (clone H5C6, Biolegend) and 4 uL APC conjugated anti-CD9 (clone H19a ...
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bioRxiv - Immunology 2021Quote: ... CD11a (M17/4, Biolegend), CD27 (LG-7F9) ...
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bioRxiv - Immunology 2021Quote: ... and IL-4 (BioLegend) were prepared in PBS at concentrations of 1000 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... CD11a (M17/4; Biolegend), and CD69 (H1.2F3 ...
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bioRxiv - Immunology 2022Quote: ... IL-4 (BioLegend, 574004), IL-13 (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with 2% FBS PBS before incubating with a panel of previously titrated 14 barcoded oligo-conjugated antibodies (BioLegend TotalSeq-C), including FITC-anti-CD45 ...
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bioRxiv - Microbiology 2020Quote: ... approximately 2-10 million total human lung cells were resuspended in MACS buffer and blocked with Human TruStain FcX (Biolegend cat# 422032) for 15min at 4°C followed by HTII-280 antibody (Terrace Biotech ...
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bioRxiv - Cancer Biology 2020Quote: ... catalog number L100-L115-01) in PBS with 1% BSA and incubated the beads with 3.5 µg/ml biotinylated anti-CD81 antibody (BioLegend, clone Eat-2) at the room temperature for 40 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... and incubated 20-60 minutes at room temperature and continued by adding 2 mL of 1X Permeabilization Buffer (Biolegend Cat. No. 421002) and centrifuged at 400-600 x g for 5 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: Enzyme-linked immunosorbent assays were completed using and according to the instructions found with the ELISA MAX™ Deluxe Set Human IL-2 (BioLegend #431804).
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bioRxiv - Immunology 2020Quote: ... Cells were then permeabilised with Saponin 2% in PBS for 20 min at room temperature and then stained in Saponin 2% in PBS with the appropriate antibodies: Th-1 (anti-CD3-BV510 (1:100, Biolegend, Cat#300448), anti-CD4-PE (1:100 ...
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bioRxiv - Immunology 2021Quote: ... prior to staining with the mAbs against cytokines (PE anti-IFN-γ, AF488 anti-TNF-α and PE-Cy7 anti-IL-2) (Biolegend) with Perm/Wash buffer (BD Biosciences) ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μL of CytoC stain buffer (2% Tween20, 10% BSA (w/v) in PBS) + 1:400 Alexa Fluor 647 anti-cytochrome c antibody (Biolegend, cat#612310) + 1:100 DAPI (1 mg/mL ...
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bioRxiv - Immunology 2021Quote: ... PE-conjugated anti-mouse TNF-α (clone MP6-XT22) and Pe-Cy7–conjugated anti-mouse IL-2 (clone JES6-5H4) antibodies (BioLegend, UK) for 20 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... lymph node FNA samples collected 2 or 8 weeks post-boost were stained for 30 min on ice with purified CD16 (3G8, BioLegend, 1:100), CD32 (FUN-2 ...
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bioRxiv - Bioengineering 2023Quote: ... the serum samples collected at 6 hours and 48 hours after administration were diluted 2-fold for ELISA analysis using the LEGEND MAX™ Rat IL-6 ELISA Kit with pre-coated plate (Biolegend, USA) according to the manufacturer’s recommendations with a little modification as described below ...
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bioRxiv - Bioengineering 2023Quote: ... The slides were then incubated in the primary antibodies (rat anti-PECAM-1, clone 390, EMD Millipore; rabbit anti-NG-2 chondroitin sulfate, EMD Millipore; rabbit anti-Keratin-14, BioLegend) diluted 1:100 in the blocking solution overnight at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and incubated in FACS buffer (1xPBS, 2%FCS) with the following anti-human antibodies: CD19-BV785 (BioLegend, 1:10), CD3-APC-eFluor 780 (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were stained for 30min in flow buffer (PBS, 1mM EDTA, 2% FBS) with antibodies against CD8α-FITC (53-6.7; Biolegend unless otherwise noted), CD19-PerCP/Cy5.5 (1D3) ...
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bioRxiv - Microbiology 2022Quote: Cytokine/chemokine concentrations in supernatant from mock and SARS- CoV-2 infected lung AT2 and cardiac cells were analyzed using the LEGENDplex human anti-virus response panel (Biolegend, Cat. 740390) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and anti-CD45.2 APC antibodies together with TotalSeq-C anti-mouse hashtag antibodies (anti-CD45 clone 30-F11, anti-H-2 clone M1/42, Biolegend, #155869 (MH5), #155871 (MH6) ...
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bioRxiv - Bioengineering 2023Quote: ... and stained with the following antibodies in FACS staining buffer (PBS with 2% Fetal Bovine Serum, 1% Penicillin-Streptomycin): anti-human CD31 (BioLegend, WM59, FITC), anti-human CD45 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Cells were suspended in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302, 1:400) and stained with antibodies at a 1:400 dilution against the following mouse markers ...
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Most L1CAM is not associated with extracellular vesicles in human biofluids and iPSC–derived neuronsbioRxiv - Neuroscience 2024Quote: ... EBs were plated on Geltrex™-coated 6-well plates (10 EBs/well) in APEL™2 medium supplemented with VEGF (40 ng/ml) (BioLegend), SCF (50 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... AML cell lines and PBMCs from healthy individuals and AML patients were stained with HERV-K102 Env-TM autoantibody and the secondary antibody PE F(ab’)2 Goat anti-human IgG Fcγ (BioLegend, Cat 398004), as well as PE Mouse IgG1 ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were incubated for 30 min at 370C with 1:500 2-NBDG and 1:1000 live-dead stain Zombie NIR (Biolegend, cat.no. 423106) in cell culture media with either high or low insulin concentration ...
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bioRxiv - Neuroscience 2024Quote: ... CTPI-2 toxicity was assessed by treating assembloids with 50uM CTPI-2 for 24hours prior to dissociation and staining with an Annexin V Cell Viability assay (Biolegend #422201 & #640935) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... 50 μL of interleukin-4 (IL-4, 50 ng/mL, BioLegend, cat. 574002) and M-CSF (100 ng/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... and TIM-3 (F38-2E2) (Biolegend) were used to evaluate T cell phenotype ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 3:20,000 anti-CD28 (BioLegend 302913), and 1:10,000 (30 IU/mL ...
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bioRxiv - Bioengineering 2021Quote: ... LAG-3 BV421 (Biolegend clone 11C3C65), PD-1 PE-Cy7 (Biolegend clone EH12.2H7).
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-Galectin-3 (BioLegend, 126702), goat anti-Galectin-3 (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... anti-GATA-3/BV421 (Biolegend, USA), anti-IL-4/BV510 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... anti-Tim-3 (F38-2E2, Biolegend), anti-IFN-γ (4S.B3 ...
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bioRxiv - Immunology 2021Quote: ... OKT-3 stock (BioLegend Inc, USA) was diluted to 50mg/ul ...