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Citations for BioLegend :
9951 - 10000 of 10000+ citations for Mouse Anti Human Papilloma virus type 16 716 325 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... staining was performed for 30 minutes at 4 C with both 70 nM AlexaFluor647-conjugated HER2 and 1ng/μL anti-DYKDDDDK (anti-FLAG) Tag Antibody-PE (Biolegend, 637310). The 5A12 library was first incubated with either 0.5 nM VEGF (Acro ...
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bioRxiv - Immunology 2024Quote: ... in FACS buffer and stained with anti-hCD19-PE-Cy7 and anti-hCD138-PE-Cy7 (Biolegend, #302216 and #356513, 1:100). After washing ...
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bioRxiv - Genomics 2024Quote: ... hfCas13 knockdown activity was confirmed by flow cytometry using APC anti-CD46 and FITC anti-CD55 (BioLegend 352405 and 311306, respectively). Monoclonal doxycycline-inducible RfxCas13d-NLS HEK293FT ...
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bioRxiv - Immunology 2019Quote: ... PD-L1 and CD80 were stained by anti-PD-L1 PE (eBioscience, catalog 14-5983-82) and anti-CD80 PE (Biolegend, catalog 305208) and their expression levels were quantified using the QUANTUM™ R-PE MESF kit (Bangs Laboratories Inc ...
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bioRxiv - Immunology 2022Quote: TruStain FcX™ PLUS antibody was used to block samples then biotin anti-lineage antibodes and biotin anti-Flk1 (Biolegend, clone 89B3A5) antibody were used to stain cells ...
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bioRxiv - Immunology 2022Quote: ... anti-Pan-gδ PE-Vio770 (clone 11f2; Miltenyi Biotech, Bergisch Gladbach, Germany) and anti-Vδ2 BV711 (clone B6; Biolegend, San Diego, CA) for 60 minutes at room temperature ...
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bioRxiv - Pathology 2024Quote: ... then incubated with primary antibody anti-IBA1 (Wako; 011-27991; dilution 1:100) and anti-Amyloid Beta1-42 (Biolegend; 805501; 1:500) prepared in 1% BSA for overnight at 4°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... OD600 of induced cultures was taken and used to determine approximate volumes of culture to stain two million cells with fluorescently labeled antibodies (anti-FLAG PE, Biolegend, cat# 637309, anti-HA Alexa 647, Biolegend, cat #682404). Cells were washed in PBS 0.5% BSA (0.5% BSA ...
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bioRxiv - Systems Biology 2024Quote: ... following staining with anti-Phospho-STING (Ser366) (D8K6H) Rabbit mAb (Alexa Fluor® 488 Conjugate) (CST, #41622) and anti-HA Alexa647 (Biolegend, #682404) at 1:200 dilution.
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bioRxiv - Cancer Biology 2021Quote: NK cells were isolated from C57BL/6 mice (male and female) with the MojoSort mouse NK cell isolation kit (BioLegend) (purity >85 % based on the flow cytometric analysis with NK1.1 and CD49b antibodies) ...
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bioRxiv - Immunology 2021Quote: ... Antibodies for flow cytometry and cell isolation against the following proteins (all mouse unless otherwise indicated) were obtained from BioLegend, eBioscience ...
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bioRxiv - Microbiology 2022Quote: IL-1 cytokine concentrations in the sera of mice or supernatants from cultured BMDMΦ were assessed using the Legendplex mouse inflammation kit (BioLegend) following the manufacturer’s instructions as described previously (42).
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bioRxiv - Immunology 2021Quote: ... and donor-derived CD4 T cells were analyzed by flow cytometry following magnetic bead–enrichment with MojoSort Mouse CD4 T Cell Isolation Kit (BioLegend).
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bioRxiv - Immunology 2021Quote: ... CD4 T cells from the spleen and peripheral lymph nodes of C57BL/6 mice on 22-24 dpi with LCMV-c13 were harvested and enriched for CD4 T cells using a MojoSort Mouse CD4 T Cell Isolation Kit (BioLegend) prior to surface staining ...
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bioRxiv - Immunology 2020Quote: Cytokine concentrations in supernatants of in vitro cultures were analyzed by the LegendPlex Mouse Th Cytokine Panel (13-plex) (BioLegend) according to the manufacturer’s instructions and analyzed on a FACS LSR II (BD Biosciences).
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bioRxiv - Immunology 2019Quote: Splenic TFH and TFR cell were stained with antibodies against mouse CD4 (Clone: RM4-5, Biolegend, San Diego, CA, USA), CD44 (Clone:IM7 ...
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bioRxiv - Immunology 2019Quote: ... IFNγ in serum was measured by ELISA according to the manufacturer’s instructions (Mouse IFN-γ ELISA MAX™ Deluxe, Biolegend). For both assays ...
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bioRxiv - Immunology 2019Quote: ... (IRF4:rat monoclonal, eBiosciences, UK, mouse monoclonal: IRF8, eBiosciences, BATF, R&D Systems, JUN Millimark, UK, PU.1 Biolegend, UK). IRF1 staining was done using rabbit polyclonal anti-human IRF1 antibody (Abcam ...
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bioRxiv - Immunology 2021Quote: Mouse cytokines and chemokines were quantitated in graft and spleen tissue using the LEGENDplex Mouse Inflammation and custom plex panel (Biolegend) according to manufacturer’s instruction 22 ...
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bioRxiv - Immunology 2021Quote: CD3+ T cells from lung single-cell suspensions (1×107 cells) were isolated by negative selection using the MojoSort mouse CD3+ T-cell Isolation kit (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... and the membranes were probed with a rabbit polyclonal antibody generated against the FHA mature C-terminal domain (MCD)(10) and a mouse monoclonal antibody generated against an HA epitope (BioLegend). Corresponding α-rabbit and α-mouse IRDye secondary antibodies (LI-COR Biosciences ...
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bioRxiv - Immunology 2022Quote: ... and PE-conjugated anti-mouse CCR5 (a Th1 marker (27)), CCR3 (a Th2 marker (28)), or CCR6 (a Th17 marker (29, 30)) antibodies (BioLegend). Finally ...
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bioRxiv - Immunology 2022Quote: ... and PE-conjugated anti-mouse CCR5 (a Th1 marker (27)), CCR3 (a Th2 marker (28)), or CCR6 (a Th17 marker (29, 30)) antibodies (BioLegend). For the MHC ...
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bioRxiv - Cell Biology 2022Quote: ... BSA- and IgG-coated dynabeads for pre-clearing were prepared by mixing dynabeads slurry (40 μl per sample) with 500 μl PBS/3% BSA or 500 μl PBS with 2.5 μg mouse IgG1 (BioLegend, 400102) respectively ...
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bioRxiv - Immunology 2020Quote: Cytokine concentrations in culture supernatant or mouse plasma were measured with the LegendPLEX Inflammation Panel (13-plex) or the Mouse Th1 Panel (5-plex) per the manufacturer protocols (BioLegend). Quantification was done using LegendPLEX software v8.0 for Windows ...
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bioRxiv - Immunology 2022Quote: ... Naïve CD4+ T cells were isolated using the Mojosort Mouse CD4 Naïve T Cell Isolation Kit (Biolegend, San Diego, CA) using LS columns on a QuadroMACS magnet (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... IL-17A, IL-17F, IL-21, IL-22) and mouse Pro-inflammatory Chemokine Panel (CCL20, CXCL1, CCL2, CCL3, CCL4) (Biolegend) using a FACSVerse flow cytometer (BD Bioscience) ...
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bioRxiv - Immunology 2022Quote: ... and then cell surface staining was performed for 30 min at 4°C with fluorochrome-conjugated monoclonal antibodies to mouse antigens purchased from Biolegend: XCR1 ...
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bioRxiv - Immunology 2022Quote: ... LN and thymic homogenates were stained with a cocktail of antibodies against CD4-FITC and CD25-APC (for LN Tregulatory cells; Mouse Regulatory T Cell Staining Kit #1, eBioscience) or CD4-PB (Biolegend), CD8-FITC ...
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bioRxiv - Genomics 2020Quote: ... Cytokine concentrations in super-natants of in vitro cultures were analyzed by the LegendPlex Mouse Th Cytokine Panel (13-plex) (BioLegend) according to the manufacturer’s instructions and analyzed on a FACS LSR II (BD Biosciences).
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bioRxiv - Immunology 2019Quote: ... Naive CD4+ and CD8+ T cells were isolated using a negative selection cocktail containing the following biotinylated mouse antibodies (Biolegend): TCR γ/δ (clone GL3) ...
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bioRxiv - Immunology 2020Quote: ... Phenotypes of B cells before and after 6 days of culture were determined by flow cytometric analysis using mouse monoclonal antibody clones: CD19 PECy7 HIB19 (Biolegend), CD27 BV421 O323 (Biolegend) ...
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bioRxiv - Pathology 2020Quote: Bead-based multiplex assays were employed to quantify cytokines (LEGENDplex™ mouse Inflammation Panel, BioLegend Inc., San Diego, CA, USA) in plasma samples obtained from mice at pre and postinfection stages ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants were removed and analyzed for IL-2 production as a correlate for T cell activation using an ELISA MAX mouse IL-2 set (BioLegend). IL-2 values measured in supernatants from the wells containing BMDCs pulsed with proteins for different time periods were transformed into µM peptide equivalents by interpolating in a standard curve generated from the values obtained from hybridomas co-cultured with BMDCs at indicated peptide concentrations.
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bioRxiv - Developmental Biology 2019Quote: ... digested enzymatically with a papain-based solution (Sumitomo Bakelite) and then resuspended in 0.3% BSA/PBS containing primary antibodies (PE-conjugated CD133 (mouse, 1:500, BioLegend, 141204) and APC-conjugated CD24 (mouse ...
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bioRxiv - Immunology 2020Quote: ... Protein levels in plasma and skin tissue protein extracts were measured using defined analyte panels: Legendplex Mouse Inflammation Panel and Inflammatory Chemokine Panel (Biolegend).
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bioRxiv - Immunology 2021Quote: ... Induced GC B-cells were then replated onto a fresh CD40LB feeder line and cultured with mouse IL-21 (10 ng/mL; BioLegend) or mouse IL-4 for another 3 to 4 days.
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bioRxiv - Immunology 2021Quote: ... CD8+ T cells were then isolated from the spleens using the MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend), following the manufacturer’s guidelines ...
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bioRxiv - Immunology 2022Quote: Bone marrow cells from femurs and tibias were cultured in presence of 20 ng/ml mouse recombinant GM-CSF (Biolegend) for 8 days ...
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bioRxiv - Immunology 2022Quote: ... single cell suspensions of mouse lungs were washed with PBS prior to staining with Zombie NIR Fixable live/dead dye (Biolegend) for 20 min at room temperature (RT) ...
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bioRxiv - Immunology 2022Quote: IL-2 ELISA was performed using an ELISA MAX™ Deluxe Set Mouse IL-2 as described in manufacturer’s protocol (BioLegend). The O.D ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were immunolabeled in blocking solution for 1 hour at RT with antibodies against the following proteins: ALIX at 5 μg/ml (mouse; no. 634502, Biolegend); IST1 at 0.4 μg/ml (rabbit ...
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bioRxiv - Immunology 2024Quote: ... Cytokine concentration in these samples were determined using CBA mouse Inflammation Kit (BDTM) or ELISA according to the manufacturers’ instructions (Biolegend).
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bioRxiv - Immunology 2024Quote: ... Naive CD8+ T cells were isolated by negative selection using the MojoSort Mouse CD8+ Naïve T cell Isolation Kit as per manufacturer’s instructions (BioLegend, >90% purity). Live cells were counted by trypan blue dye exclusion and resuspended at a concentration of 106 cells/mL in complete T cell media (RPMI-1640 (Cytiva ...
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bioRxiv - Microbiology 2024Quote: Levels of cytokines and soluble mediators present in serum of infected mice were quantified using LEGENDplex Mouse Inflammatory Panel kit (BioLegend) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... and cultured in DMEM/F-12 medium containing 10% FBS and 20 ng/mL mouse macrophage colony-stimulating factor (BioLegend) for 7 days ...
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bioRxiv - Microbiology 2024Quote: ... The amount of Ag-specific IgG was then quantitated by running a western blot using mouse IgG (BioLegend CAT#944803) of known mass as a standard ...
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bioRxiv - Molecular Biology 2023Quote: ... Conditioned medium was collected and IL-1β levels were assessed with ELISA MAXTM Deluxe Set Mouse IL-1β (Biolegend, 432604) following manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... with recombinant IL-15/IL-15RA complex (100 ng/ml; Thermo Fischer, Waltham, MA) or recombinant mouse IL-6 (50 ng/ml, BioLegend), TGF- β1 (1ng/mL) ...