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Citations for BioLegend :
9901 - 9950 of 10000+ citations for Mouse Anti Human Papilloma virus type 16 716 325 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... T cells were isolated from spleen using MojoSort™ Mouse CD3 T Cell Isolation Kit (Biolegend Cat no; 480024) as per the manufacturers protocol ...
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bioRxiv - Immunology 2022Quote: ... CD8+ OT-I splenic T cells were isolated from crushed spleens of naïve OT-I mice using the Mouse CD8 T cell Isolation Kit (BioLegend). Sorted tumor APC populations were pulsed with 0.01 ng/mL SIINFEKL peptide (Invivogen ...
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bioRxiv - Immunology 2022Quote: ... Naïve CD4+ T cells were isolated from the spleen by using the MojoSort Mouse Naïve CD4+ T Cell Isolation Kit (#480040, BioLegend). CD4+ T cells isolated from the OT-II spleen were cocultured with BMDCs ...
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bioRxiv - Cell Biology 2022Quote: ... 1µg/mL for FC) APC mouse IgG1, k isotype Control (400120, 1µg/mL for FC) were purchased from Biolegend.
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bioRxiv - Cancer Biology 2024Quote: ... The cells were stained for VEGF-A using antibodies against mouse VEGF-A (cat# 512809, clone 2G11-2A05, Biolegend) and AlexaFluor647-conjugated secondary antibodies ...
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bioRxiv - Immunology 2024Quote: ... which is RPMI-1640-based and supplemented with 5 μg/mL recombinant mouse IL-3 (#575506 or #575508, BioLegend) as described in previous studies (6 ...
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bioRxiv - Immunology 2023Quote: Cells harvested from in vitro Th17 differentiated cells were stained with the following antibodies against mouse CD3 (17A2, Biolegend), CD4 (GK1.5 ...
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bioRxiv - Immunology 2023Quote: ... and CD4+ cells were magnetically enriched using mouse negative selection CD4+ T cell isolation kits (STEMCELL Technologies and Biolegend). Live Tregs were sorted as fixable viability dyeneg (Thermo Fisher Scientific and Tonbo Biosciences) ...
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bioRxiv - Microbiology 2023Quote: ... MBL) or H-2q-restricted LCMV NP118 peptide (MBL) and 50 U/mL of recombinant mouse IL-2 (BioLegend) for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... expanded CD4+ T cells were cocultured at different ratios with CFSE/CTV labeled CD8+ T cells and stimulated with Mouse T-Activator CD3/CD28 Dynabeads at 2 cells per bead ratio and 0.5ng/mL IL-2 (BioLegend). Cell culture media consisted with RPMI 1640 ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatants were used to measure cytokines and chemokines using LEGENDplex™ Mouse Inflammation Panel (13-plex) (BioLegend 740446). In the case of CSF cell transplant ...
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bioRxiv - Immunology 2023Quote: ... followed by 100ul of Streptavidin nanobeads for 1×10^8 cells in 1ml of MACS buffer for 15min on ice (MojoSort Mouse pan B cell isolation kit II, Cat.no. 480088, Biolegend). The cell suspension was diluted and put on a pre-rinsed LS Column (Cat ...
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bioRxiv - Cancer Biology 2023Quote: Single-cell suspensions from tumors or CD3 T cells isolated using MojoSort Mouse CD3 T Cell Isolation Kit (Biolegend) following the manufacture’s protocols ...
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bioRxiv - Immunology 2023Quote: ... NOD female mice were injected intraperitoneally (i.p.) with recombinant mouse IL-33 (0.5-1ug/mice/day, in 100uL of PBS; BioLegend: 580508) or vehicle only (PBS ...
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bioRxiv - Immunology 2023Quote: Listed below are the antibodies used for flow cytometric analysis of mouse cell-surface molecules: ① Purchased from Biolegend(USA): Anti-CD45-BV510(clone 30-F11);Anti-Ly-6G/Ly-6c(Gr-1)-PerCP(clone RB6-8C5);Anti-CD4-BV421(clone GK1.5);Anti-F4/80-FITC(clone BM8);Anti-CD8a-PE(clone 53-6.7) ...
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bioRxiv - Immunology 2023Quote: IL-6 secretion was quantified using an ELISA MAX Deluxe Set Mouse IL-6 according to the manufacturer’s instructions (431304, Biolegend). Total and NP-specific antibody levels and IL-2 secretion were measured by ELISA on half-area 96-well plates (Greiner Bio-One ...
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bioRxiv - Immunology 2023Quote: ... and around 1×106 cells were blocked with purified mouse CD16/32 antibody (1:100 dilution; cat. #101301; BioLegend) for 5 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... and IL-22 were measured in the culture supernatants using the LEGENDPlex Mouse T Helper Cytokine Panel kit (BioLegend) following manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... congenic CD45.1 mouse spleen was isolated and red blood cells were lysed with RBC lysis buffer (BioLegend, CA, USA). Leukocytes were then resuspended in complete RPMI with 1µg/mL high molecular weight Poly(I:C) ...
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bioRxiv - Immunology 2023Quote: ... CD4+ OT-II KN2 cells were purified from CD45.1.2 OT-II KN2 donor mice with the MojoSort™ Mouse CD4 T Cell Isolation Kit (Biolegend) and labeled with cell proliferation dye eFluor 450 (0.01 mM ...
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bioRxiv - Immunology 2023Quote: ... Enriched CellTrace Violet-labeled OT-I CD8+ T cells were resuspended at 20,000 cells/100uL in complete RPMI + 10ng/mL of recombinant mouse IL-2 (Biolegend). As positive control ...
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bioRxiv - Immunology 2023Quote: ... CD73- CD4+ CD25- Foxp3- newly generated CD4SP thymocytes were sorted from Foxp3eGFP-Cre-ERT2 reporter mice and reconstituted at a concentration of 20,000 cells/100uL in complete RPMI + 100ng/mL recombinant mouse IL-7 (Biolegend). CD4SP thymocytes and DC subsets were co-cultured at a 2:1 ratio in round-bottom 96-well plates for 4 days and then analyzed by flow cytometry on day 5 to evaluate the percentage of Tregs generated in vitro.
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bioRxiv - Cell Biology 2024Quote: ... untouched primary naïve T cells were isolated with an EasySep Mouse T cell Isolation Kit according to the manufacturer’s protocol (BioLegend). The purified CD4+ Naïve T cells were stimulated with 20 ng/mL phorbol 12-myristate13-acetate (PMA ...
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bioRxiv - Immunology 2024Quote: ... for the clone that binds to the high affinity IL-2 receptor (CD25): we incubated 0.5ugr mouse Il-2 (PepreoTech) to 5ugr of an IL-2 mAb (JES6-1A12, Biolegend) for 30 minutes on ice ...
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bioRxiv - Cancer Biology 2024Quote: Neutrophils were isolated from the femurs of MMTV-PyMT tumour bearing mice using MojoSort Mouse Neutrophil Isolation Kit (Biolegend), with neutrophil retention via negative selection strategy ...
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bioRxiv - Cancer Biology 2024Quote: Neutrophils were isolated from the blood of MMTV-PyMT tumour bearing mice using MojoSort Mouse Neutrophil Isolation Kit (Biolegend), with neutrophil retention via negative selection strategy ...
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bioRxiv - Neuroscience 2024Quote: The following primary antibodies were used for immunoblotting: mouse monoclonal antibodies against TuJ1 (BioLegend Cat# 801202 (also 801201, 801213), RRID:AB_10063408 ...
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bioRxiv - Immunology 2024Quote: Skin cytokines or growth factors were quantified using LegendPlex Mouse Cytokine release syndrome and Hematopoietic stem cell kits (Biolegend). Briefly ...
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bioRxiv - Bioengineering 2024Quote: ... Supernatants were collected (spun down at 10,000 x g for 15 mins) for cytokine analysis using LegendPlex 13-plex Mouse Inflammation Panel (Biolegend). Adhered cells were washed with 1x PBS and collected using 0.25% trypsin ...
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bioRxiv - Immunology 2024Quote: The following antibodies were used for staining mouse tissue sections for imaging or isolated cells for flow cytometry: B220 PCPCy5.5 (clone RA3-6B2; Biolegend), B220 PE/Fire 700 (clone RA3-6B2 ...
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bioRxiv - Immunology 2024Quote: Proinflammatory chemokines of normal liver and liver micrometastasis were detected using LEGENDplex™ Mouse Proinflammatory Chemokine Panel (BioLegend, #740451). Briefly ...
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bioRxiv - Microbiology 2024Quote: Western and immunofluorescence assays (IFA) were probed using the following antibodies: mouse monoclonal antibody (mAb) HA.11 (Biolegend; 901515), rabbit polyclonal antibody (pAb ...
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bioRxiv - Neuroscience 2024Quote: ... cells were placed on ice for 15 mins and treated with cold Mouse monoclonal antibody against LAMP1(#328611; Biolegend) specific to the N terminal luminal epitope of the antigen ...
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bioRxiv - Immunology 2021Quote: ... and cells were stained with 1.5 μg/ml Brilliant Violet 605™ anti-CD16 (clone: 3G8) and 1 μg/ml APC anti-CD18 (clone: 1B4/CD18) (Biolegend). Cell data was collected using an Attune flow cytometer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... After 48 h incubation, cells were collected and stained with anti-CD3-FITC (BioLengend, 300406) and anti-CD69-APC (BioLegend, 310910) for 30 min at 4 °C ...
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bioRxiv - Bioengineering 2021Quote: ... PBMCs were collected and stained using APC/Fire anti-CD4 and APC anti-CD8 (Supplemental Table 1) (Biolegend, San Diego CA). PBMCs were first washed and stained with Zombie Yellow (Supplemental Table 1 ...
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bioRxiv - Cell Biology 2021Quote: NK92 and YTS cell lines were incubated for 20 mins at 37°C and 5% CO2 on #1.5 glass coverslips that had been pre-coated with anti-CD18 (clone IB4, 10 μg/ml) and 10 μg/ml anti-NKp30 (clone P30-15, Biolegend, NK92) or anti-CD28 (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... WT and CD56 KO NK92 and YTS cells were incubated for 20 mins at 37°C and 5% CO2 on .5 glass coverslips that had been pre-coated with 10 μg/ml anti-CD18 (clone IB4) and anti-NKp30 (clone P30-15, Biolegend, NK92) or anti-CD28 (clone CD28.2 ...
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bioRxiv - Immunology 2021Quote: ... permeabilized with saponin 0.2% for 20 min and stained with PE-Cy7-anti-IFN-γ mAb and/or PE-anti-IL-10 mAbs (Biolegend). For TF and GzB/PRF staining ...
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bioRxiv - Cell Biology 2020Quote: ... anti-CD9 (clone VJ1/20.3.1; kindly provided by Francisco Sánchez, Madrid, Spain) and anti-CD63 (H5C6; BioLegend, San Diego, CA, USA). Band intensities were analysed using Image J.
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bioRxiv - Microbiology 2021Quote: ... Tissues were incubated with primary antibodies (mouse anti-HuC/D IgG clone 16A11 at 1:200 [Thermofisher], rabbit polyclonal anti-tubulin β-3 IgG at 1:500 [Biolegend]) in PBS containing 1% Triton X-100 for 48 h at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-human CD47 antibody conjugated to APC (323123) and anti-HA Alexa Fluor® 488 anti-HA.11 Epitope Tag (901509) were purchased from Biolegend. The antibody to ORF3a was a gift from Prof ...
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bioRxiv - Immunology 2020Quote: ... anti-CD45.1 (Clone A20, Catalog # 110728), and anti-CD45.2 (Clone 104, Catalog # 109808) antibodies were obtained from Biolegend (San Diego, CA) for analysis of mouse immune profiles.
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bioRxiv - Cancer Biology 2022Quote: ... Cells staining positive for anti-CD11b-Pacific blue (Miltenyi, 130-110-802) and anti-F4/80-APC Cy7 (Biolegend. cat.123117) were sorted as macrophages ...
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bioRxiv - Immunology 2024Quote: ... then treated with fluorophore-conjugated secondary antibody (donkey anti-rabbit IgG Alexa Fluor 488, Invitrogen A32790TR; goat anti-hamster IgG Alexa Fluor 594, BioLegend B388503) diluted 1:1000 in blocking buffer for 1hr at RT ...
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CD56/NCAM mediates cell migration of human NK cells by promoting integrin-mediated adhesion turnoverbioRxiv - Cell Biology 2023Quote: ... at 37°C and 5% CO2 on #1.5 glass coverslips that had been pre-coated with 10 µg/ml anti-CD18 (clone IB4) and anti-NKp30 (clone P30-15, Biolegend; 325202) or 5 µg/ml Fc-ICAM-1 (Enzo Life Science ...
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bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (CD45+ immune cells: 1:50 anti-CD45-VioGreen [Miltenyi Biotec, Germany]; CD31+ endothelial cells: 1:2000 anti-CD31-PE [BioLegend, CA]/1:50 anti-CD45-VioGreen [Miltenyi Biotec ...
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bioRxiv - Genetics 2023Quote: ... Recombinant Anti-AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474, Purified anti-Tubulin β-3 (TUBB3) Antibody 1:1000 (Biolegend, # PRB-435P), anti-GAPDH antibody 1:1000 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... non-fat dried skimmed milk powder and then probed with ICSM35 anti-PrP antibody (D-Gen Ltd; 1:5000 for RML) or with 6D11 anti-PrP antibody (BioLegend (808004); 1:5000 for ME7 ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... cells were resuspended in FACS buffer and incubated with Alexa Fluor® 488 anti-CD31 ([390] 102414) and APC/Cyanine7 anti-CD45 ([30-F11] 103116) antibodies (1/100, BioLegend) and mouse TruStain FcXTM (1/200 ...