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Citations for BioLegend :
951 - 1000 of 1854 citations for Rat CD254 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Co-culture supernatant was collected following completion of culture and analyzed for IL-2 using the ELISA MAX™ Deluxe Set for human IL-2 (Biolegend, 431804) at a dilution of 1:5.
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bioRxiv - Microbiology 2023Quote: ... PBMCs were then plated as described above and human IL-10 was measured in the cell supernatants by ELISA (Biolegend, Cat. # 430604).
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bioRxiv - Molecular Biology 2024Quote: ... IL-6 and IL-15 was quantified in cell supernatants using a sandwich ELISA Max Deluxe Set Human (Biolegend, San Diego, CA), according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... The blot was then washed 3 times in PBS-T and probed with goat anti-rat HRP (Biolegend) and goat anti-mouse HRP (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... C57BL/6 or BALB/c mice were treated with 100μg in 200μl PBS of anti-CD20 mAb (5D2, Genentech) or isotype control (Rat IgG2a, Biolegend) i.v ...
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bioRxiv - Immunology 2019Quote: ... C57BL/6 mice were treated with 100μg in 200μl PBS of anti-CD20 mAb (5D2, Genentech) or isotype control (Rat IgG2a, Biolegend) i.v ...
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bioRxiv - Immunology 2021Quote: ... Germinal center B cells were labeled using rat anti-GL7 antibody (FITC; BioLegend, catalog no. 144604, 1:250). Tfh cells were labeled using anti-CD4 antibody (BioLegend ...
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bioRxiv - Molecular Biology 2022Quote: ... Alexa Fluor® 647-conjugated rat anti-mouse CD104 (integrin β4) antibody (1:100, BioLegend, clone 346-11A), and FITC-conjugated rat anti-mouse Ly6d (1:100 ...
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bioRxiv - Molecular Biology 2022Quote: ... and were stained with APC/Cy7-conjugated rat anti-mouse CD45 antibody (1:200, BioLegend, clone 30-F11), Alexa Fluor® 488-conjugated or PE/Cy7-conjugated rat anti-mouse CD326 (EpCAM ...
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bioRxiv - Cell Biology 2019Quote: ... F4/80+ cells were then isolated by positive selection using a rat anti-F4/80 antibody (Biolegend, 123120) and sheep anti-rat Dynabeads (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... 3× before being incubated with APC-conjugated rat anti-CD83 antibody (1.25 μL/10×106 cells; Biolegend, 121510) for 30 minutes on ice ...
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bioRxiv - Immunology 2019Quote: ... Sections were washed three times with PBS and a secondary anti-rat IgG AlexaFluor488 antibody (BioLegend, London, UK) was used for detection ...
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bioRxiv - Immunology 2020Quote: ... The following antibodies were used: Alexa Fluor 700 Rat anti-mouse CD3 (17A2, BioLegend, San Diego, CA, USA), Pacific Blue Rat anti-mouse CD4 (GK1.5 ...
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bioRxiv - Bioengineering 2022Quote: ... and then 1:200 dilution of Alexa Fluor 488 mouse anti-rat CD45 (BioLegend, San Diego, CA, USA), PE mouse anti-rat CD31 (BD biosciences ...
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bioRxiv - Immunology 2022Quote: ... The cells were then incubated for 10 minutes with purified rat anti-mouse CD16/CD32 (Biolegend, 93 clone)(only for mouse cells ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with rat–anti-Ly6G (1.25 μg/mL on-slide, 0.67 μg/mL free-floating, 127602; Biolegend), anti-Iba1 (2 µg/mL on-slide ...
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bioRxiv - Microbiology 2023Quote: ... Sections were washed in PBS and surface antibodies were detected with anti-Rat AlexaFluor-488 secondary antibody (BioLegend). After surface staining ...
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bioRxiv - Immunology 2023Quote: ... P008_108)52 for 1h at 4°C followed by PE-rat anti-human IgG Fc (1:200, Biolegend) for 45 min at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... Unconjugated antibodies were incubated for 1 hour (1:100) on ice in the dark (Rat IgG2a (Biolegend, 400501), Rat IgG2a (Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies included a combination of the following: rat anti-CD4 antibody (Biolegend, UK, cat# 100505, 1:500), guinea pig anti-IBA1 antibody (Synaptic Systems ...
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bioRxiv - Immunology 2021Quote: ... cells were restimulated with cognate antigens for three days and then supernatants were collected for IL-17A and IFNγ detection with ELISA (Biolegend, San Diego, CA). For determination of IL-17A+ and IFNγ+ CD45.1+ OT-II CD4+ T cell percentages ...
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bioRxiv - Genomics 2020Quote: ... Tissues were immunostained with the primary antibodies rat anti-mouse CD31:Biotin (clone 390, Biolegend, San Diego, CA, USA) and Living Colors DsRed Polyclonal Antibody (rabbit anti-E2-Crimson ...
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bioRxiv - Immunology 2019Quote: ... HBcAg and VSV-G antibodies listed above followed by rat monoclonal anti-mouse IgG1 conjugated to PE-Cy7 (BioLegend), goat anti-mouse IgG2a conjugated to AlexaFluor® 647 and goat anti-rabbit conjugated to AlexaFluor® 488 (ThermoFisher ...
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bioRxiv - Biochemistry 2019Quote: ... IHC was performed to rat or human tissue with the following panel of antibodies: pan-Aβ (Biolegend, Cat.# 800709,), pE3-Aβ (Cell Signaling Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... Hosts were culled at 4 weeks and engraftment frequencies were estimated by flow cytometry using only naked mole-rat markers not cross-reactive with mouse cells and CD45.1 (A20, BioLegend). Engraftment rates were adjusted for input cell dose to 100k/Tx ...
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bioRxiv - Neuroscience 2020Quote: ... APC-conjugated rat monoclonal anti-mouse CD11b and PerCP-Cy5.5-conjugated mouse monoclonal anti-human CD200 (all Biolegend, UK) on ice in the dark for 30 min ...
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bioRxiv - Biochemistry 2021Quote: The following antibodies were used in this study: Anti-ZBP1 clone RonoB19 (WB 1:500, ref. 651602, Rat, BioLegend), anti-TIAR (C-18 ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... 8 or 12 weeks and engraftment frequencies were estimated by flow cytometry using only naked mole-rat markers not cross-reactive with mouse cells and CD45.1 (A20, BioLegend). Engraftment rates were adjusted for input cell dose to 100k/Tx ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was collected by centrifugation at 15,000g for 15 min and incubated with Rat anti-FLAG beads (BioLegend) for 2h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The cell suspension was stained using allophycocyanin (APC)/Cy7-conjugated rat anti-mouse CD45 antibody (1:200, BioLegend, #101319), PE-conjugated rat anti-mouse EpCam antibody (1:500 ...
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bioRxiv - Neuroscience 2019Quote: ... in Alexa Fluor® 488) and rat anti-mouse CD31 (1:100; Biolegend 390 in APC/Fire™ 750) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Following antibodies were used for flow cytometry (all 5µg/ml, except indicated differently): rat anti mouse CD45 (clone 30-F11, BioLegend), rat anti mouse CD11b (clone M1/70) ...
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bioRxiv - Immunology 2022Quote: ... female C57BL/6 mice were treated with 20 μg of α-CCR2 mAb (clone: MC21, RRID:AB_2314128; (23) or isotype control antibody (Rat IgG2b, Biolegend) in 200 μl of PBS via daily intra-peritoneal (i.p. ...
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bioRxiv - Neuroscience 2023Quote: ... cat no: 130-123-284) and allophycocyanin (APC) conjugated rat anti-mouse CD45 (1:100, Biolegend, cat no: 103111) in blocking solution for 30 minutes on ice in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... For further experiments viable CD45highCD8+ cells were labeled with rat anti-CD45 APC (1:100, catalog no. 103111; BioLegend), rat anti-CD8 PerCP/Cyanine5.5 (1:100 ...
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bioRxiv - Immunology 2023Quote: ... injected at 0.5 mg per mouse: rat anti-mouse CD210 (IL-10R, clone 1B1.3a, BioLegend, Cat# 112712, Lot# B360179,) and rat IgG1 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed once with cold HMI-9 and resuspended in 200μl cold HMI-9 with rat anti-mouse IgM-FITC (1:500, Biolegend). After one wash with cold HMI-9 ...
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bioRxiv - Immunology 2023Quote: ... Immunostaining was performed using the following primary antibodies: rat anti-mouse CD8α (clone 53-6.7, eBioscience) and Armenian hamster anti-mouse CD11c (clone N418, BioLegend). CD8 primary staining signal was further amplified using an anti-rat IgG secondary antibody conjugated with Alexa Fluor (AF ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were labelled with rat-anti-Prominin-1 antibody (Brilliant Violet 421-conjugated; BioLegend, #141213, Lot# B255870, 1:1000) and Alexa Fluor 647-conjugated recombinant human EGF (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... T lymphocytes were evaluated using the following antibodies: rat anti-mouse IL-17A (TC11-18H10.1, 1/50; Biolegend, #506922), rat anti-mouse RORγt (AFKJS-9 ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were washed three times with TBS-T (TBS with 0.1% Tween-20) and bound Anxs were detected by immunoblotting using Rat anti-FLAG antibody (1:5000; Biolegend) and ECL (ThermoFisher).
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bioRxiv - Cancer Biology 2024Quote: ... washed and re-suspended in the following primary antibodies: a rat monoclonal (IgG2a) anti-mouse FRβ (BioLegend,1:100) or a sheep polyclonal anti-mouse FRα (R&D Systems ...
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bioRxiv - Bioengineering 2024Quote: ... Supernatants were spun down to remove cell debris and frozen at -80 °C until analysis using the ELISA MAX™ Deluxe Set Human IFN-γ (BioLegend catalog #430104) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... total thymocytes were processed to single cell suspension in 10% FBS/HBSS (20×106 cells/ml) and incubated with rat anti-mouse CD24 antibodies (M1/69, Biolegend) (30 μg/100 ×106 cells ...
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bioRxiv - Immunology 2021Quote: ... cells were washed twice in FACS buffer and incubated with rat anti-human Fc antibody conjugated to allophycocyanin (Cat: 410712, BioLegend) for 15 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... CD3 localization was detected using FITC conjugated streptavidin (CALTAG Laboratories) and PSGL-1 localization was detected using anti-Rat IgG-PE (BioLegend) following a 30 minute incubation at 4°C ...
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bioRxiv - Immunology 2019Quote: ... (IRF4:rat monoclonal, eBiosciences, UK, mouse monoclonal: IRF8, eBiosciences, BATF, R&D Systems, JUN Millimark, UK, PU.1 Biolegend, UK). IRF1 staining was done using rabbit polyclonal anti-human IRF1 antibody (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... we performed a multianalyte bead-based immunoassay (LEGENDplex™ Custom Panel of Rat Inflammation markers; Biolegend, San Diego, CA, USA) to provide fast and quantitative information on the WD and NRG1 treatment effects in levels of relevant pro and anti-inflammatory cytokines and chemokines ...
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bioRxiv - Biophysics 2020Quote: ... Nup107 in HeLa Kyoto mEGFP-Nup107 cells were labelled with an Alexa Fluor 647 anti-GFP rat monoclonal primary antibody (Clone FM264G, BioLegend) for dSTORM imaging (Figure 4a ...
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bioRxiv - Cancer Biology 2022Quote: ... for 24h at 4°C on a shaker and then stained with antibodies (rat anti-CD3 [clone 17A2, cat#100202, Biolegend] ...