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Citations for BioLegend :
901 - 950 of 1854 citations for Rat CD254 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: The cytokines IFNγ and TNFα contained in supernatants derived from 1-4 h BxPC3 - CAR T cell co-cultures were quantified by ELISA using Abs from Biolegend.
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bioRxiv - Immunology 2020Quote: ... IFN-γ or GM-CSF release in the cell culture supernatant was measured using ELISA according to the manufacturer’s protocol (Sanquin and BioLegend, respectively).
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bioRxiv - Immunology 2021Quote: Organ homogenates were thawed and the resulting supernatants analyzed for cytokines and proteins by ELISA (Biolegend, BD, and R&D) and Luminex (R&D ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants were removed and analyzed for IL-2 production as a correlate for T cell activation using an ELISA MAX mouse IL-2 set (BioLegend). IL-2 values measured in supernatants from the wells containing BMDCs pulsed with proteins for different time periods were transformed into µM peptide equivalents by interpolating in a standard curve generated from the values obtained from hybridomas co-cultured with BMDCs at indicated peptide concentrations.
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bioRxiv - Immunology 2020Quote: ... The supernatants were collected and the amount of IL10 in the supernatants were measured using the Human IL10 ELISA MAX (Biolegend) kit according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... The samples were then flash frozen and defrosted for 4 rounds to lyse the cells and used on an IL-10 detection ELISA (Catalog 430601, Biolegend).
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bioRxiv - Immunology 2020Quote: ... Cytokine content of cell free supernatants was determined using standard sandwich ELISA according to the manufacturer’s instructions (IFN-γ and GM-CSF; Sanquin and Biolegend). Some target cells were pre-incubated for 2 days in the presence or absence of UGCG inhibitors.
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bioRxiv - Immunology 2022Quote: ... TNF and IL-2 in the supernatant of in vitro activated cells and murine IL-10 in the serum were determined using ELISA MAX Deluxe Set (BioLegend) according to manufacturer instructions.
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bioRxiv - Microbiology 2024Quote: ... TNF-α production was quantified in the supernatant of RAW 264.7 cells using a sandwich ELISA Max Deluxe Set (Biolegend) according to the manufacturers protocol.
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bioRxiv - Molecular Biology 2023Quote: ... Conditioned medium was collected and IL-1β levels were assessed with ELISA MAXTM Deluxe Set Mouse IL-1β (Biolegend, 432604) following manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... Secreted levels of IFN-β were measured from BMDM supernatants twenty-four hours following the start of the infection following the Sandwich ELISA protocol published by BioLegend. Anti-IFN-β capture antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed in RLT buffer and supernatants were collected for analysis using ELISA (Total Tgf-β1 Legend MAX and Free Active Tgf-β1 Legend MAX, Biolegend). The limit of detection was 8 pg/mL for Tgf-β1.
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bioRxiv - Immunology 2023Quote: ... and GM-CSF (purified anti-mouse GM-CSF; biotin anti-mouse GM-CSF) ELISA antibodies and standards were purchased separately from Biolegend.
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bioRxiv - Immunology 2023Quote: ... Tgf-β concentration was measured using ELISA (Total Tgf-β1 Legend MAX and Free Active Tgf-β1 Legend MAX, Biolegend). The limit of detection was 8 pg/mL for Tgf-β1 ...
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bioRxiv - Immunology 2023Quote: ... Serum samples were assessed for titers of different antibody isotypes using LegendPlex Mouse Immunoglobulin Isotyping Panel and IgE ELISA (BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... IL-8 concentration in medium samples was measured by using the ELISA MAX Deluxe Set Human IL-8 (BioLegend, 431504) according to the user manual.
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bioRxiv - Neuroscience 2024Quote: Aggregated α-synuclein was quantified in PBS- and TrX-soluble tissue fractions (5µg total protein/well) using the LEGEND MAXTM α-Synuclein Aggregate ELISA (BioLegend) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... was from BioXCell (Lebanon, NH), and rat anti-murine CD3 (Cat no 100331, LEAF purified) from Biolegend (San Diego, CA).
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bioRxiv - Developmental Biology 2021Quote: ... rat anti-mouse Ly-6A/E (Sca1) (labelled with BV510) (BioLegend – 108129; 5 µl per million cells) and rat anti-lineage cocktail (labelled with BV421 ...
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bioRxiv - Cancer Biology 2022Quote: ... was used as the primary antibody and rat anti-human IgG Fc antibody conjugated to APC (BioLegend) was used as the secondary antibody ...
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bioRxiv - Immunology 2019Quote: ... Fc-Block was performed (10µg/ml purified rat anti-mouse CD16/CD32, Biolegend TruStain FcX anti-mouse), after which cells were stained with Fixable Viability Staining e780 (1:1000 ...
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bioRxiv - Immunology 2019Quote: ... anti-rat IgG1/2a/2b/2c-Biotin combined with Streptavidin-HRP Streptavidin-HRP (all BioLegend, London, UK).
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bioRxiv - Neuroscience 2019Quote: ... and cells were stained with rat anti-mouse ICAM-1 (1:100; Biolegend YN1/1.7.4 in PerCP), rat anti-mouse CD45 (1:100 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed with PBS5 and stained with APC-conjugated goat anti-rat IgG (Biolegend, 1:200) for 20 min ...
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bioRxiv - Developmental Biology 2021Quote: ... single-cell suspensions were incubated 20min on ice in 500µL FACS buffer containing 3.5µg/mL of rat anti-mouse EpCAM antibody conjugated with Alexafluor 647 (Biolegend) and 8µg/mL mouse anti-β2-Microglobulin monoclonal antibody conjugated with PE (Santa Cruz) ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated with secondary antibody diluted in 3% BSA (Alexa Fluor 594 Goat anti-Rat IgG, BioLegend, #405422) in the dark for 1 hr at room temperature ...
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bioRxiv - Immunology 2020Quote: ... the same lysates were processed using LEGENDplex™ Mouse/Rat Free Active/Total TGF-β1 Assay (BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The following antibodies were used for labeling cells: PE rat anti-mouse CD45 (Clone 30-F11; BioLegend), FITC rat anti-mouse CD3 (Clone 17A2 ...
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bioRxiv - Immunology 2023Quote: ... for further 20 min on ice with the following antibodies: rat anti-mouse CD19 PE (Biolegend, 115507), rat anti-mouse CD138 FITC (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... rat anti-mouse CD68 in FITC at 1:300 for 30 minutes (BioLegend®, Cat. No. 137005) using Intracellular Fixation & Permeabilization Buffer (eBioscience ...
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bioRxiv - Microbiology 2023Quote: ... GC B cells were labeled using rat anti-GL7 antibody (FITC; BioLegend, catalog no. 144604, 1:250). Tfh cells were labeled using anti-CD4 antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Alexa Fluor 647 or FITC–conjugated rat anti-mouse CD326 (Ep-CAM) antibody(1:200; Biolegend, G8.8), PE/Cyanine5 anti-mouse CD86 antibody(1:200 ...
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bioRxiv - Microbiology 2023Quote: ... samples were treated with rat anti-mouse CD16/32 antibody (1:1000, clone 93, BioLegend ref. 101302) at 4°C for 15 min to block Fc receptors ...
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bioRxiv - Developmental Biology 2023Quote: The following antibodies were used for FACS: PE/Cy7 rat anti-CD45 (CD45, 1:250, 103114, BioLegend), PE rat anti-epithelial cadherin (ECAD ...
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bioRxiv - Physiology 2024Quote: ... and immunofluorescence staining for PECAM-1 (AlexaFluor488 labelled primary monoclonal rat antibody, 5μg mL-1, MEC13.3, BioLegend) and S100A9 (Cy5.5 directly labelled ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then stained with APC anti-rat CD90/mouse CD90.1 (Thy-1.1) Antibody (BioLegend, 1:260) for 20 min ...
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bioRxiv - Immunology 2024Quote: Surface staining for flow cytometry: PE-Cy7 rat anti-mouse CD4 (clone RM4-5, BioLegend, cat# 100528) (48) ...
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bioRxiv - Immunology 2024Quote: ... cells were stained on ice with the following antibodies (all 1:500): rat isotype PE (BioLegend 400507), CD80 PE (eBioscience 12-0801-82) ...
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bioRxiv - Cancer Biology 2024Quote: ... or Anti-human/mouse CD49f antibody (#313620, Biolegend) (isotype control, Pacific Blue Rat IgG2a, k; #400527, Biolegend). For GD3 FACS analysis ...
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bioRxiv - Immunology 2022Quote: The concentration of IFN-γ was determined in the supernatant of 6×106 cells from each donor (6 healthy donors) using the Human IFN-γ ELISA deluxe set (BioLegend) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... TNFα and IL-23 expression in the culture medium was examined using an ELISA (Invitrogen for TNFα Biolegend for IL-23).
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bioRxiv - Bioengineering 2024Quote: ... were coated with 100 µL of 4 µg/mL polyclonal anti-lysostaphin antibody (AICBIOTECH-PAb102) in 1×ELISA coating buffer (BIOLEGEND-421701). Wells were washed with 0.05% Tween 20 (SIGMA-P9416-50ML ...
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bioRxiv - Immunology 2024Quote: ... that were pulsed with epitope (10 ng/mL) was collected and IFN-γ production was measured with an enzyme-linked immunosorbent assay (ELISA, BioLegend) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and IL-2 were investigated with an enzyme-linked immunosorbent assay (ELISA MAXTM Deluxe Set, Biolegend®, San Diego, CA, USA) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants thereof were removed and analyzed for IL-2 production as a correlate for T-cell activation using an ELISA MAX mouse IL-2 set (BioLegend, SanDiego, USA).
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bioRxiv - Immunology 2020Quote: ... The cytokine production was measured using the ELISA Deluxe Set Human IL-8/CCL20/IL-1β (Biolegend, San Diego, California, United States), following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2021Quote: Enzyme-linked immunosorbent assays were completed using and according to the instructions found with the ELISA MAX™ Deluxe Set Human IL-2 (BioLegend #431804).
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bioRxiv - Microbiology 2022Quote: Corning ELISA plate wells were coated with 100µL of 100 μg/mL FCoV2 whole virus antigen or 100 μg/mL SCoV2 RBD or FCoV RBD antigen in sodium bicarbonate ELISA coating buffer at pH 9.5 (BioLegend, San Diego, CA) and incubated overnight at RT ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected 3 days after coculture initiation and used to quantify T cell activation levels with ELISA (BioLegend, Cat no. 430801) following the manufacturer’s protocols.
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bioRxiv - Microbiology 2023Quote: Cytokine levels in the serum and spleen of mice infected with SPN were measured using ELISA MAX™ Standard Set Mouse IL-6 (BioLegend 431301) and ELISA MAX™ Standard Set Mouse IL-1β (BioLegend 432601 ...