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Citations for BioLegend :
901 - 950 of 1007 citations for Recombinant Rat Il1b His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... XER mice were injected intraperitoneally at 1000h and 1800h on GD3 with either PBS or 10µg recombinant Lif (554006, BioLegend, San Diego, CA) followed by blue dye injection and dissection at GD4 1200h ...
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bioRxiv - Cancer Biology 2023Quote: ... the tumor organoids were treated with the immune checkpoint inhibitor Nivolumab (Selleckchem, Cat#A2002) and the Ultra-LEAF Purified Human IgG4 Isotype Control Recombinant Antibody (Biolegend, Cat#403701) at a concentration of 10 μg/mL ...
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bioRxiv - Genomics 2020Quote: ... Tissues were immunostained with the primary antibodies rat anti-mouse CD31:Biotin (clone 390, Biolegend, San Diego, CA, USA) and Living Colors DsRed Polyclonal Antibody (rabbit anti-E2-Crimson ...
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bioRxiv - Immunology 2019Quote: ... HBcAg and VSV-G antibodies listed above followed by rat monoclonal anti-mouse IgG1 conjugated to PE-Cy7 (BioLegend), goat anti-mouse IgG2a conjugated to AlexaFluor® 647 and goat anti-rabbit conjugated to AlexaFluor® 488 (ThermoFisher ...
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bioRxiv - Biochemistry 2019Quote: ... IHC was performed to rat or human tissue with the following panel of antibodies: pan-Aβ (Biolegend, Cat.# 800709,), pE3-Aβ (Cell Signaling Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... Hosts were culled at 4 weeks and engraftment frequencies were estimated by flow cytometry using only naked mole-rat markers not cross-reactive with mouse cells and CD45.1 (A20, BioLegend). Engraftment rates were adjusted for input cell dose to 100k/Tx ...
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bioRxiv - Neuroscience 2020Quote: ... APC-conjugated rat monoclonal anti-mouse CD11b and PerCP-Cy5.5-conjugated mouse monoclonal anti-human CD200 (all Biolegend, UK) on ice in the dark for 30 min ...
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bioRxiv - Biochemistry 2021Quote: The following antibodies were used in this study: Anti-ZBP1 clone RonoB19 (WB 1:500, ref. 651602, Rat, BioLegend), anti-TIAR (C-18 ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... 8 or 12 weeks and engraftment frequencies were estimated by flow cytometry using only naked mole-rat markers not cross-reactive with mouse cells and CD45.1 (A20, BioLegend). Engraftment rates were adjusted for input cell dose to 100k/Tx ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was collected by centrifugation at 15,000g for 15 min and incubated with Rat anti-FLAG beads (BioLegend) for 2h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The cell suspension was stained using allophycocyanin (APC)/Cy7-conjugated rat anti-mouse CD45 antibody (1:200, BioLegend, #101319), PE-conjugated rat anti-mouse EpCam antibody (1:500 ...
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bioRxiv - Neuroscience 2019Quote: ... in Alexa Fluor® 488) and rat anti-mouse CD31 (1:100; Biolegend 390 in APC/Fire™ 750) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Following antibodies were used for flow cytometry (all 5µg/ml, except indicated differently): rat anti mouse CD45 (clone 30-F11, BioLegend), rat anti mouse CD11b (clone M1/70) ...
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bioRxiv - Cell Biology 2021Quote: ... Unfractionated or c-Kit-enriched BM cells were then incubated with purified rat anti-mouse lineage antibodies (CD3, BioLegend, 100202 ...
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bioRxiv - Immunology 2022Quote: ... female C57BL/6 mice were treated with 20 μg of α-CCR2 mAb (clone: MC21, RRID:AB_2314128; (23) or isotype control antibody (Rat IgG2b, Biolegend) in 200 μl of PBS via daily intra-peritoneal (i.p. ...
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bioRxiv - Neuroscience 2023Quote: ... cat no: 130-123-284) and allophycocyanin (APC) conjugated rat anti-mouse CD45 (1:100, Biolegend, cat no: 103111) in blocking solution for 30 minutes on ice in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... For further experiments viable CD45highCD8+ cells were labeled with rat anti-CD45 APC (1:100, catalog no. 103111; BioLegend), rat anti-CD8 PerCP/Cyanine5.5 (1:100 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed once with cold HMI-9 and resuspended in 200μl cold HMI-9 with rat anti-mouse IgM-FITC (1:500, Biolegend). After one wash with cold HMI-9 ...
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bioRxiv - Immunology 2023Quote: ... Immunostaining was performed using the following primary antibodies: rat anti-mouse CD8α (clone 53-6.7, eBioscience) and Armenian hamster anti-mouse CD11c (clone N418, BioLegend). CD8 primary staining signal was further amplified using an anti-rat IgG secondary antibody conjugated with Alexa Fluor (AF ...
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bioRxiv - Immunology 2023Quote: ... injected at 0.5 mg per mouse: rat anti-mouse CD210 (IL-10R, clone 1B1.3a, BioLegend, Cat# 112712, Lot# B360179,) and rat IgG1 ...
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bioRxiv - Microbiology 2023Quote: ... T lymphocytes were evaluated using the following antibodies: rat anti-mouse IL-17A (TC11-18H10.1, 1/50; Biolegend, #506922), rat anti-mouse RORγt (AFKJS-9 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were labelled with rat-anti-Prominin-1 antibody (Brilliant Violet 421-conjugated; BioLegend, #141213, Lot# B255870, 1:1000) and Alexa Fluor 647-conjugated recombinant human EGF (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were washed three times with TBS-T (TBS with 0.1% Tween-20) and bound Anxs were detected by immunoblotting using Rat anti-FLAG antibody (1:5000; Biolegend) and ECL (ThermoFisher).
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bioRxiv - Cancer Biology 2019Quote: ... washed once with PBS at concentration 2×10^6/mL in RPMI1640 containing 10% FBS and 100U/ml of recombinant human IL-2 (BioLegend, San Diego, CA). After 72h ...
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bioRxiv - Biochemistry 2022Quote: Moles of product formed in hydrolysis reactions used for ACE2 activity and specificity profiling were determined by comparing area under the curve for LC chromatograms corresponding to these reactions to areas under the curve observed for control reactions in which > 95% input substrate conversion was achieved by incubation of substrate with 25 nM recombinant human ACE2 commercial standard (BioLegend, San Diego, CA) for between one and four hours pending peptide substrate ...
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bioRxiv - Neuroscience 2020Quote: ... cells were treated with M-CSF (100ng/ml) Recombinant Human M-CSF (Macrophage-Colony Stimulating Factor, BioLegend cat. 574806, San Diego, CA, USA) for 5 min ...
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bioRxiv - Immunology 2021Quote: BMDMs were generated by culturing total BM cells for 7 days in complete RPMI supplemented with 10 ng/mL recombinant mouse M-CSF (BioLegend, Cat# 576406M-CSF). C8-S cells (ATCC ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were passed through 70-micron cell strainer and subsequently cultured 10mL complete RPMI media supplemented with 20 ng/mL of recombinant murine M-CSF (576406; BioLegend, 315-02 PeproTech). On day 3 ...
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bioRxiv - Immunology 2024Quote: ... anti-mouse IL4 (10 µg/mL) and recombinant mouse IL2 (5 ng/mL) and IL12 (10 ng/mL) (BioLegend, San Diego, CA, US), for three days prior to use.
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bioRxiv - Immunology 2021Quote: ... total thymocytes were processed to single cell suspension in 10% FBS/HBSS (20×106 cells/ml) and incubated with rat anti-mouse CD24 antibodies (M1/69, Biolegend) (30 μg/100 ×106 cells ...
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bioRxiv - Immunology 2021Quote: ... cells were washed twice in FACS buffer and incubated with rat anti-human Fc antibody conjugated to allophycocyanin (Cat: 410712, BioLegend) for 15 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... CD3 localization was detected using FITC conjugated streptavidin (CALTAG Laboratories) and PSGL-1 localization was detected using anti-Rat IgG-PE (BioLegend) following a 30 minute incubation at 4°C ...
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bioRxiv - Immunology 2019Quote: ... (IRF4:rat monoclonal, eBiosciences, UK, mouse monoclonal: IRF8, eBiosciences, BATF, R&D Systems, JUN Millimark, UK, PU.1 Biolegend, UK). IRF1 staining was done using rabbit polyclonal anti-human IRF1 antibody (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... we performed a multianalyte bead-based immunoassay (LEGENDplex™ Custom Panel of Rat Inflammation markers; Biolegend, San Diego, CA, USA) to provide fast and quantitative information on the WD and NRG1 treatment effects in levels of relevant pro and anti-inflammatory cytokines and chemokines ...
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bioRxiv - Biophysics 2020Quote: ... Nup107 in HeLa Kyoto mEGFP-Nup107 cells were labelled with an Alexa Fluor 647 anti-GFP rat monoclonal primary antibody (Clone FM264G, BioLegend) for dSTORM imaging (Figure 4a ...
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bioRxiv - Cancer Biology 2022Quote: ... for 24h at 4°C on a shaker and then stained with antibodies (rat anti-CD3 [clone 17A2, cat#100202, Biolegend] ...
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bioRxiv - Molecular Biology 2022Quote: ... Alexa Fluor® 488-conjugated or PE/Cy7-conjugated rat anti-mouse CD326 (EpCAM) antibody (1:200, BioLegend, clone G8.8), Alexa Fluor® 647-conjugated rat anti-mouse CD104 (integrin β4 ...
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bioRxiv - Physiology 2022Quote: ... Samples were incubated with anti-c-kit rat monoclonal antibody (1:500 v/v, ACK2, BioLegend, San Diego, CA, USA) overnight (4ºC) ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were washed again 3 × 5 minutes in PBS and incubated overnight with rat anti-phospho-TDP primary antibody (1:100, 829901, BioLegend), in a dilution buffer (0.05% Tween ...
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bioRxiv - Developmental Biology 2022Quote: ... hPGCLCs were dissociated into single cells and then stained with Alexa Fluor 647-conjugated rat anti-human PDPN (337007, BioLegend) and BV421-conjugated anti-CD49f (INTEGRINα6 ...
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bioRxiv - Immunology 2020Quote: ... Randomized NOD/SCID mice were then administered the following treatments for two weeks post-adoptive transfer: (i) PBS and rat isotype control IgG2b antibodies (RTK4530; BioLegend), (ii ...
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bioRxiv - Immunology 2020Quote: ... the cremaster muscles were stained by using primary antibodies including rabbit anti-mouse laminin α5 (antibody 405) or anti-mouse laminin α4 (antibody 377) and Brilliant Violet 411-conjugated rat anti-mouse MHC II (Biolegend); monocytes were identified by CX3CR1GFP expression ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed with PBS for three times and incubated with rat anti-mouse IgG2a conjugated with APC (407109, BioLegend) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Unfractionated or c-Kit-enriched BM cells were then incubated with purified rat anti-mouse lineage antibodies (CD3, BioLegend, 100202; CD4, eBioscience, 16-0041-82; CD5, BioLegend, 100602; CD8, BioLegend, 100702 ...
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bioRxiv - Cell Biology 2021Quote: ... Unfractionated or c-Kit-enriched BM cells were then incubated with purified rat anti-mouse lineage antibodies (CD3, BioLegend, 100202; CD4, eBioscience, 16-0041-82; CD5, BioLegend, 100602 ...
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bioRxiv - Immunology 2020Quote: ... washed with FACS buffer and then stained with a secondary goat anti-rat IgG DyLight 649 (BioLegend Cat# 405411, RRID:AB_1575141) antibody and then washed with FACS buffer and blocked with 5% normal rat serum ...
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bioRxiv - Immunology 2021Quote: ... and finally with a mixture of AlexaFluor 647-conjugated rat anti-mouse Ly6C antibody (BioLegend, clone HK1.4; diluted 1:500) and DAPI (Molecular Probes ...
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bioRxiv - Immunology 2022Quote: Circulating CD20+ cells were depleted by intraperitoneal administration of 200 μg of rat-anti-mouse-CD20-depleting antibody (Biolegend, 152104) two days before the start of TAM administration ...
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bioRxiv - Cell Biology 2022Quote: ... were performed after confirming full differentiation into mature MCs using flow cytometric analysis of lineage-specific c-KIT (rat anti-CD117 Brilliant Violet 421-conjugated, 1:200, Biolegend) and FcεRI (armenian hamster anti-FcεRIα Alexa Fluor 647-conjugated ...
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bioRxiv - Cell Biology 2022Quote: ... Full differentiation into mature MCs was confirmed by expression of lineage-specific c-KIT (rat anti-CD117 Brilliant Violet 421- conjugated, 1:200, Biolegend) and FcεRI (armenian hamster anti-FcεRIα Alexa Fluor 647- conjugated ...