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Citations for BioLegend :
801 - 850 of 1065 citations for Recombinant Rat Il1b His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... C57BL/6 or Rag2-/- mice were treated intranasally (i.n.) with 100 ng of recombinant IL-33 (Biolegend) each day for 5 consecutive days ...
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bioRxiv - Immunology 2022Quote: ... clones were screened by their loss of ability to produce IP-10 to recombinant IFNβ (Biolegend #581302).
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bioRxiv - Immunology 2021Quote: Timed-pregnant mice dams were intravenously injected with up to 10 μg of recombinant mouse cytokines (Biolegend) at gestation day 13.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend) [9] ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend). The viability of NALM6-GL cells was then assessed by adding 150 ug / mL D-Luciferin (PerkinElmer ...
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bioRxiv - Biochemistry 2021Quote: Quadruplicate samples of WT HEK293T and CCT5-FLAG tagged cells were lysed and purified using anti-FLAG affinity matrix (Biolegend cat no. 651501) using 20mM HEPES pH 7.5 and 100mM NaCl wash buffer and 200mM Glycine/150mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... followed by washing and incubated with a solution of AlexaFluor® 647 conjugated anti-His antibody (BioLegend #652513, 10 µg/mL). After washing and drying ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 WT biotinylated RBD protein (His & AVI Tag) conjugated with Brilliant Violet 605 Streptavidin and TotalSeq-C0973 Streptavidin (BioLegend, 405275) and TotalSeq-C0974 Streptavidin(BioLegend ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 5 μL 7-AAD (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated with PE-streptavidin (BioLegend, 405204), 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 500xg for 5 minutes and then resuspended in 50uL of PBS+ 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302). Cells were labelled with antibodies as described below ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with Zombie-violet or Zombie-NIR in PBS at 1:2000 dilution for 5 minutes at room temperature prior to resuspending in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302) for blocking ...
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bioRxiv - Systems Biology 2020Quote: ... respective concentrations (ranging from 25 ng/mL to 100 ng/mL) of recombinant human IL-8 (Biolegend, 715404) and/or recombinant human CCL2 (Peprotech ...
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bioRxiv - Cell Biology 2022Quote: ... Bone marrow cells were differentiated into BMDMs using 40 ng/mL recombinant M-CSF (BioLegend, catalog number 576406) in the same media formulation as TR-AMs ...
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bioRxiv - Cancer Biology 2022Quote: RAW 264.7 cells or BMDMs were cultured as described above and then treated with recombinant mouse TGFβ1 (Biolegend). Fifteen minutes later ...
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bioRxiv - Immunology 2021Quote: Mice were injected intravenously under isoflurane anesthesia once daily with 1×105 U recombinant mouse IFNγ (575306, Biolegend) in 150 μL PBS ...
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bioRxiv - Immunology 2020Quote: ... The skin sheets were then transferred into another 1ml medium containing 0.1 μg of recombinant mouse 6Ckine (BioLegend) to enhance DC-migration ...
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bioRxiv - Microbiology 2024Quote: ... Activation of MAPK and NF-κB signaling pathways was triggered using human recombinant TNF-α (BioLegend, Cat. 570102). Detailed information concerning experimental procedure is given in supplementary material.
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bioRxiv - Immunology 2024Quote: ... and 10 ng/ml recombinant macrophage colony-stimulating factor (M-CSF; Cat# 576402; BioLegend, San Diego, CA, USA) for 7 days ...
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bioRxiv - Immunology 2023Quote: ... freshly sorted cells were stained ex vivo or after stimulation with carrier-free recombinant mouse IL-25 (Biolegend) at a concentration of 100 ng/ml in complete RPMI medium for 36 h ...
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bioRxiv - Cell Biology 2023Quote: ... 720-IC-200) or 1 μg/ml Fc-VCAM-1 (Recombinant Human VCAM-1-Fc Chimera, Biolegend, 553706) was added to the chamber slide ...
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bioRxiv - Cancer Biology 2024Quote: ... or basal media supplemented with 10 ng/ml of either recombinant IL1α or IL1β (Biolegend, 570004 and 579404) then fixed and processed as below.
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bioRxiv - Cancer Biology 2019Quote: ... PerCP/Cy5.5-conjugated rat anti-mouse CD45 mAb (clone 30-F11, BioLegend, San Diego, CA), FITC-conjugated rat anti-mouse CD68 mAb (clone FA-11 ...
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bioRxiv - Immunology 2021Quote: Macrophages (~3 x 106) were stained with anti-F4/80 (rat monoclonal FITC-conjugated, BioLegend) in a 1:200 dilution in FACS buffer for 20 minutes on ice ...
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bioRxiv - Immunology 2021Quote: Splenocytes and LPLs were incubated with a rat anti-mouse CD16/CD32 mAb (BioLegend, USA) for 30 min to block Fc receptors ...
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bioRxiv - Developmental Biology 2021Quote: ... and rat anti-mouse CD31 (labelled with AF647) (BioLegend – 102516; 0.25 µg per million cells) in staining buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... and rat anti-lineage cocktail (labelled with BV421) (BioLegend – 133311; 5 µl per million cells) in staining buffer and washed twice ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... Ki67 antibodies (mouse: clone 16A8; naked mole-rat: clone Ki-67; both PE-conjugated, BioLegend) were added at 5µl/1x106 cells and incubated for 30min at room temperature in the dark ...
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bioRxiv - Cell Biology 2021Quote: ... purified rat anti-mouse MHC class II or MHC class II Alexa 488 (BioLegend, 107602,107616), purified rat anti-mouse CD45 (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for mouse cells are rat anti-mouse CD8a conjugated to FITC (BioLegend, 100706) and rat anti-mouse CD19 conjugated to PE (BioLegend ...
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bioRxiv - Neuroscience 2021Quote: ... rat anti-LAMP1 (DSHB, 1D4B; 1:250) and mouse anti-Neurofilament (BioLegend, 837801; 1:500); and the following secondary antibodies ...
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bioRxiv - Molecular Biology 2019Quote: ... and PE-conjugated rat-anti mouse Ly-6A/E (#108108) antibodies were purchased from Biolegend. LS columns ...
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bioRxiv - Cell Biology 2021Quote: ... followed by staining with Alexa Fluor 488 Goat anti-rat IgG (BioLegend, 405418, 1:500) and Alexa Fluro 647 Goat anti-moue IgG (BioLegend ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Freshly isolated dFB from rat skin was stained with zombie violet viability dye (Biolegend, 423114) to stain dead cells ...
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bioRxiv - Cell Biology 2022Quote: ... PE Rat anti-Mouse/Human CD44 (Monoclonal; clone IM7; Biolegend; 1:100; Cat#103007, FACS). BUV395 Rat anti-Mouse CD45 (Monoclonal ...
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bioRxiv - Cell Biology 2022Quote: ... BV421 Rat anti mouse CD68 (Monoclonal; clone FA-11; Biolegend; 1:300; Cat#137017, FACS). AlexaFluor700 Rat anti mouse CD206 (Monoclonal ...
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bioRxiv - Cell Biology 2022Quote: ... flushed bone marrow cells were stained with rat anti-mouse F4/80 BV711 (Biolegend, 123147) and rat anti- mouse CD11b FITC (Biolegend ...
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bioRxiv - Physiology 2024Quote: ... BioLegend (valid) # 137001 ) sections were incubated with a rat anti-mouse CD68 antibody (BioLegend (valid) #137001 ...
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bioRxiv - Genomics 2023Quote: ... cells were incubated in the antibody mix (2 μg/ml rat anti-CD326 (BioLegend 312502), 1 μg/ml rabbit anti-RAD51-AF647 (Bioacademia 70-012) ...
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bioRxiv - Immunology 2023Quote: ... The following primary antibodies were used: rat monoclonal anti-CD31 (1:250, MEC13.3, Biolegend, #102502), goat polyclonal anti-CD206 (1:250 ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated with anti-SSEA-3 rat IgM antibody (1:1000, BioLegend, 330302) at 4°C for 1 h following incubation with fluorescein (FITC ...
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bioRxiv - Immunology 2023Quote: ... cells were treated with TruStain FcX rat anti-mouse CD16/32 antibodies (BioLegend; cat # 101320) or human TruStain FcX CD16/32/64 antibodies (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... and rat anti-mouse CD90.2 (conjugated with FITC, clone#30-H12, cat# 105305, Biolegend, Inc). Dead cells were excluded by staining with Viability-Dye (conjugated with eFluor 506 ...
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bioRxiv - Immunology 2023Quote: ... The cells were then incubated overnight with rat anti-CD14 IgG (clone Sa14-2; BioLegend) and goat anti-GST IgG (Rockland ...
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bioRxiv - Immunology 2024Quote: ... Neutrophils and macrophages were stained using Alexa647 conjugated rat anti-Ly6G (Biolegend #127609, 1:100) and Alexa561 conjugated rat anti-F4/80 (Invitrogen #505-4801-80 ...
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bioRxiv - Biochemistry 2020Quote: ... incubated with secondary antibody (1/100 dilution of chicken anti-HIS-FITC polyclonal from Immunology Consultants Laboratory; or 1/250 anti-human IgG-APC clone HP6017 from BioLegend) for 30 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... Cells were suspended in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302, 1:400) and stained with antibodies at a 1:400 dilution against the following mouse markers ...
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bioRxiv - Cancer Biology 2022Quote: ... or TFH mimicking signals (including 0.02 μg/mL recombinant human IL-4, Peprotech; 0.05 μg/ml recombinant human IL-21, Peprotech, and 5 μg/mL anti-human CD40, Biolegend). Complete RPMI medium contained RPMI 1640 (Gibco ...
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bioRxiv - Immunology 2022Quote: ... neutrophils were incubated with autologous activated platelets (1:10 ratio) (65) for 2.5h and human recombinant CCL28 (50 nM) (BioLegend), the CCR3 antagonist SB328437 (10 mM ...
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bioRxiv - Pathology 2022Quote: ... or B cell supernatant pre-treated with a recombinant antibody to deplete IL-10 (IgG2b, clone JES5-16E3, Biolegend). As controls ...