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Citations for BioLegend :
901 - 950 of 1188 citations for Phospho Tyrosine Rabbit Polyclonal Biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... The knock-out of the T cell receptor (TCR) was assessed with the absence of signal after staining 1:200 with APC-labeled anti-CD3ε (Biolegend). Each CAR construct contained a Strep tag which allowed for a two-step staining to validate successful knock-in ...
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bioRxiv - Immunology 2021Quote: FACS purified cell subsets were labeled by incubation for 30 minutes at 4°C with TotalSeq anti-mouse Hashtag antibodies (BioLegend), then washed ...
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bioRxiv - Immunology 2021Quote: ... Cells were labeled with live/dead dye (Zombie Aqua) for 10 min at RT and blocked with human TruStain FcX (BioLegend) for 20 min at RT ...
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bioRxiv - Microbiology 2021Quote: ... Cells were labeled with the following antibodies directly conjugated to the following fluorophores: CD45-PE/Cy7 (clone 30-F11, BioLegend), CD11b-BV510 (clone M1/70 ...
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bioRxiv - Cancer Biology 2021Quote: ... For the assessment of CD71 expression in TF-1 and DAMI cells were labeled with the phycoerythrin (PE)-cyanine 7 (Cy7)-conjugated CD71 (BioLegend) (#113811) ...
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bioRxiv - Cancer Biology 2021Quote: ... For the assessment of CD11b expression MDS92 and MDS-L cells were labeled with the phycoerythrin (PE)-cyanine 7 (Cy7)-conjugated CD11b (BioLegend) (#557743) ...
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bioRxiv - Cancer Biology 2021Quote: ... The following anti-mouse antibodies we used for surface staining: Alexa Fluor 647-labeled H-2Kb/H-2Db – MHC Class I (clone 28-8-6, Biolegend); PE-labeled I-A/I-E – MHC Class II (Clone M5/114.15.2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and resuspended in 100 μL Binding Buffer containing 100 nM M1 anti-FLAG antibody labeled with Alexa Fluor 488 and Alexa Fluor 647 anti-human IgG Fc (BioLegend) diluted 1:100 (v/v) ...
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bioRxiv - Biophysics 2022Quote: ... were established by transfection of pIRES-HA-CXCR4 and pIRES-HA-CXCR4-W1955.34L and subsequent cell sorting for equivalent surface expression levels using 1:1000 Alexa Fluor 488-labeled anti-HA antibody (clone 16B12, Biolegend). U87 cells were grown in Dulbecco’s modified Eagle medium (Themo Fischer Scientific ...
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bioRxiv - Immunology 2022Quote: ... The nanoparticles were washed again and finally resuspended in the 100 µL of assay buffer containing fluorescently labeled AlexaFlora568-detection antibody for IFNγ (Biolegend).
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bioRxiv - Bioengineering 2022Quote: ... and then stained with the fluorescently labeled antibody that detects the corresponding cell surface receptor (anti-EGFR-BV421, Biolegend, Cat: 352911; anti-HER2-BV421, Biolegend, Cat ...
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bioRxiv - Immunology 2020Quote: Recipient mice were infused with 2-3 x 106 purified donor T cells that were labeled with 5 μM CFSE (BioLegend) as previously described63 ...
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bioRxiv - Immunology 2020Quote: ... CD4+CD25high Treg cells were enriched from PBMCs after staining with PE-cyanine 5 (PE-Cy5)-labeled anti-CD25 Ab (BioLegend), followed by positive selection using anti-PE and anti-Cy5 microbeads (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: Alexa fluor 488 or Alexa fluor 594 or FITC or Alexa fluor 648 labeled anti-His-tag (MBL or Biolegend),
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bioRxiv - Immunology 2022Quote: ... 5×104 Tconv (CD4+CD25-) cells from TFAM control were labeled with CFSE Cell Division Tracker Kit (Cat# 423801, BioLegend) and cultured with mitomycin-treated wild type splenocytes ...
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bioRxiv - Immunology 2019Quote: ... cells were washed once in in 1ml of PBS-1%FCS (8’, 350rcf).Cells were then separately labeled with hashtag antibodies (Biolegend TotalSeq-A antibodies ...
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bioRxiv - Immunology 2021Quote: ... phagocytosis was stopped by washing with 4°C PBS and centrifugation at 1200 RPMI before the cells were stained with APC-labeled anti-CD11b (Clone M1/70, Biolegend) to identify human macrophages and Live/Dead stain (Fixable Viability Dye ...
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bioRxiv - Immunology 2021Quote: Viability of stimulated B cells were assessed by flow cytometry using FITC-labeled annexin V and propidium iodide (PI) (Biolegend). For apoptosis assays by live-imaging ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then labeled according to the manual of the FITC ANNEXIN V Apoptosis Detection Kit with 7-AAD (BioLegend) with a modification where the amount of 7-AAD was doubled.
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bioRxiv - Cell Biology 2020Quote: ... Cells were then washed with FACS buffer and labeled with PE conjugated mouse anti-human TNFRSF12A antibody (clone ITEM-4, Biolegend) for 30 minutes on ice ...
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bioRxiv - Cell Biology 2020Quote: ... The virus particles were stained at a concentration of 5×108 particles mL−1 and labeled with anti-GFP PE antibody (Clone FM264G, BioLegend) at 0.4 μg mL−1 in a 100 μL staining volume for a minimum of 30 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Cells within each experimental donor group were pooled and labeled with green and red CFSE respectively according to manufacturer protocol (Biolegend). Cells were counted and mixed to produce a 1:1 ratio of green and red labeled cells ...
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bioRxiv - Biochemistry 2022Quote: ... K562s were transfected according to manufacturer’s protocol with CD20 using pre-packaged lentiviral particles (G&P Biosciences LTV-CD20) and sorted for CD20-expression using rituximab and a BV421-labeled anti-human secondary (Biolegend) as the staining reagent on a FACS instrument ...
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bioRxiv - Immunology 2022Quote: ... Each of the three biological replicates that were treated with PBS vehicle control or TGM was labeled with one of three unique TotalSeq Hashtags Antibody markers (Biolegend). The samples were individually sort-purified by gating CD45+ cells and then each treatment group was pooled with an even number of cells from each sample ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and resuspended in 100 μL Buffer B containing 100 nM M1 anti-FLAG antibody labeled with Alexa Fluor 488 (In house) and Alexa Fluor 647 anti-human IgG Fc (BioLegend) diluted 1:100 (v/v) ...
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bioRxiv - Neuroscience 2023Quote: ... For further experiments viable CD45lowSiglec-H+ microglia were labeled with rat anti-ICAM1 BV711 (1:100, catalog no. 116143; BioLegend), rat anti- Ly6A/E FITC (1:100 ...
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bioRxiv - Bioengineering 2023Quote: ... Binding of T cell-secreted SiRPα-Fc on T cell CD47 was assessed by staining with labeled goat anti-human IgG Fc (HP6017, Biolegend) at 4°C for 30min ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated O/N at 4°C with a mouse anti-iNOS2 IgG antibody labeled with Alexa Fluor 564 (BioLegend)) or a mouse anti-ARG-1 IgG antibody labeled with FITC (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with cold PBS and then stained with a cocktail of fluorescently labeled antibodies including: anti-human CD3-PE-Cy7 (Clone: OKT3, Biolegend), Ghost viability dye (Tonbo Biosciences),TCR a/b-BV421 (Clone ...
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bioRxiv - Synthetic Biology 2023Quote: ... OD600 of induced cultures was taken and used to determine approximate volumes of culture to stain two million cells with fluorescently labeled antibodies (anti-FLAG PE, Biolegend, cat# 637309 ...
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bioRxiv - Immunology 2023Quote: ... 5000 cells of each compartment were labeled with anti-mouse hashtag oligonucleotide (HTO) antibodies (TotalSeq™-C0301/C0302/C0303, Biolegend) and pooled together ...
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bioRxiv - Microbiology 2023Quote: ... samples were stained with Alexa Fluor 647-labeled mouse-anti-human CD56 (10 μg/mL, Biolegend, San Diego, CA, USA) for 1 h and washed with HBSS (Sigma Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Cells were collected and stained with fluorescently labeled antibodies against CD45 PE-Cy 7 (clone: 30-F11, cat # 103114: Biolegend), TCRβ APC (clone ...
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bioRxiv - Immunology 2023Quote: ... THP-1 and SKOV-3 cell lines and primary human T cells were labeled with fluorescent antibody against human CD70 or isotype control (Biolegend) to verify antigen expression as described before35.
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bioRxiv - Immunology 2023Quote: ... Cells were centrifuged at 400 g for 8 minutes and stained with lanthanide-labeled antibodies against immunophenotypic markers in CyTOF buffer containing Fc receptor blocker (BioLegend) for 30 minutes at room temperature (RT) ...
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bioRxiv - Bioengineering 2023Quote: ... The expression of Mo surface markers was measured by flow cytometric analysis using fluorescently labeled antibodies against CD11b (BioLegend #101207), CD45R (BioLegend #103211) ...
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bioRxiv - Immunology 2023Quote: ... Lung resident cells were distinguished from those in the vasculature by exclusion from staining with fluorescently labeled anti-CD45 antibody (PerCP, clone 30F-11, BioLegend) that was injected retro-orbitally into each mouse approximately 3 minutes prior to euthanasia65 ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 x105 cells were labeled with 1 μg anti-hCD81-PE clone 5A6 or 1 µg isotype control-PE (349506, 400114; Biolegend)32 and by Western blotting (4-12% SDS-PAGE ...
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bioRxiv - Physiology 2023Quote: THP-1 monocytes (2×105 cells/mL) were labeled with 5µM cell-permeant Calcein-AM in DMSO (1:200, BioLegend, 425201) for 20 minutes at 37°C ...
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bioRxiv - Immunology 2023Quote: ... cells were labeled stained in PBS for 20 mins at 4°C with a live dead marker (Zombie UVTM, Biolegend). Surface staining was performed in brilliant stain buffer (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... samples were incubated overnight at 4°C with combinations of the following antibodies: PE-Cy7-labeled CD63 (NVG-2, BioLegend), AF532-labeled iNOS (CXNFT ...
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bioRxiv - Immunology 2023Quote: Biotinylated protein antigens were individually multimerized with fluorescently labeled streptavidin (BB515, BD Biosciences; BUV615, BD Biosciences; AF647, Biolegend; BV421, Biolegend) as described previously (25) ...
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bioRxiv - Immunology 2023Quote: Biotinylated protein antigens were individually multimerized with fluorescently labeled streptavidin (BB515, BD Biosciences; BUV615, BD Biosciences; AF647, Biolegend; BV421, Biolegend) as described previously (25) ...
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bioRxiv - Immunology 2024Quote: ... or LN single cell suspensions were incubated with fluorescently labeled antibodies or anti-LCMV Db-restricted tetramers (NIH Tetramer Core) as well as anti-CD16/32 (clone S17011E, Biolegend) in FACS buffer (phosphate buffered saline/PBS with 0.5% fetal bovine serum/FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... The cell suspensions were then labeled with lineage related antibodies: PE/Dazzle™ 594 anti-mouse/human CD11b (Biolegend 101256), APC anti-mouse Ly-6G/Ly-6C (Gr-1 ...
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bioRxiv - Molecular Biology 2024Quote: ... Surface staining was carried out using a fluorochrome-labeled PE-conjugated anti-human CD11a (HI111) monoclonal antibody obtained from BioLegend.
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bioRxiv - Immunology 2023Quote: ... 106 of the CTV labeled T cells were stimulated in vitro for 3 or 5 days in cultures containing 100ng/ml IL-15 (BioLegend) or a combination of 100ng/ml IL-7 (BioLegend ...
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bioRxiv - Neuroscience 2024Quote: ... Cell Hashing was used to label samples from distinct samples.46 Single cell suspensions were labeled with the TotalSeq Hashtag Antibodies (BioLegend) for 30 min on ice ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were then washed with FACS buffer and incubated with the following Lineage antibodies for 30 min at 4°C: CD11b Biotin (BioLegend, Catalog No. 101204, 1/1000), CD11c Biotin (BioLegend ...
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bioRxiv - Cancer Biology 2021Quote: ... rabbit-α-K10 (1:1000; 905404; Biolegend), rabbit-α-Loricrin (1:500 ...