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Citations for BioLegend :
901 - 950 of 5311 citations for Hepatitis E ORF2 protein mouse Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then stained with 2.5 μl of PE anti-human E-cadherin (50 μg/ml; Biolegend, 324106) or matched concentration of PE Mouse IgG1 ...
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bioRxiv - Biochemistry 2020Quote: ... and isotype controls (mouse IgG-FITC, mouse IgG2a-APC; BioLegend). Then ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse mIgD PE/CY7 and mouse IFNγ APC/CY7 (Biolegend). FVD506 and 7AAD were used to distinguish viable cells ...
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bioRxiv - Bioengineering 2023Quote: ... mouse anti-human/mouse Grazyme B-PE Cyanine 7 (BioLegend), rat anti-mouse CD8-FITC (BioLegend) ...
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bioRxiv - Genomics 2022Quote: ... Cells were then resuspended in FACS buffer and incubated with a BV-421 anti-DYDDDDK epitope tag antibody (BioLegend) for 1 hr at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... IP of HA-positive ribosomes was performed by incubating with Anti-HA.11 Epitope Tag Antibody (Biolegend, Cat# 901515) for 4 hours in a microcentrifuge tube on a microtube rotator kept at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... HA (mouse; BioLegend), TGN46 (sheep polyclonal ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse TGFβ1 (Biolegend), N-acetyl cysteine (NAC ...
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bioRxiv - Immunology 2021Quote: Freshly thawed decidual immune cells (1-2 10e6 cells) were incubated with Fc blocker (Human TruStain FcX, Biolegend) in PBS with 1% BSA for 10 minutes at 4C and then surface stained with CD45-FITC (HI30 ...
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bioRxiv - Immunology 2022Quote: ... cells were pre-treated with 2.5 μg/mL anti-CD16/32 (Fc Block; 2.4G2; BioLegend, San Diego, CA) for 15 minutes before continuing with surface staining ...
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bioRxiv - Immunology 2021Quote: ... 1×105-1×106 cells were stained after Fc-blocking with TruStain FcX (BioLegend, San Diego, CA, USA) and incubation with Zombie Aqua fixable viability dye (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants were aspirated and cells were then treated with Fc block (clone 93, Ref: 101302, BioLegend, 1:1000) at 4°C for 15 min ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice after the primary stain and incubated with PE–anti-human Fc antibody (Biolegend, 410708) in MACS buffer for 20 min on ice ...
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bioRxiv - Physiology 2022Quote: ... 3 106 cells were then incubated with Fc receptor blocking solution (Human Trustain FcX, Biolegend, San Diego, USA) for 5 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in FACS buffer (PBS + 4% FBS) supplemented with Fc block (clone 93; BioLegend Cat. 101320) and incubated at 4°C for 5 minutes before staining ...
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bioRxiv - Immunology 2019Quote: ... an Fc block step was performed by resuspending cells in 50μl of Fc block prepared in PBS/5%BSA (room temperature) using 5μl of Human Trustain FcX (Biolegend) per sample i.e ...
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bioRxiv - Cancer Biology 2019Quote: ... Fc receptors were blocked by incubation with 2.5 μg/mL anti-CD16/32 antibody (clone 93; 101320, BioLegend) in staining buffer (25 mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... Fc-blocked cells were initially stained with FITC-conjugated anti-CD4 and BV421-conjugated anti-CD25 (102034; BioLegend) antibodies ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation with 50 ng/mL anti-Fc-γ III/II (CD16/32) receptor Ab (93, BioLegend) in 100 μl of FACS Buffer (1% FBS and 1 mM EDTA in PBS) ...
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bioRxiv - Microbiology 2022Quote: ... 107 cells per sample were then incubated with 10 μl of Fc-blocker (anti-CD16/CD32 antibody, Biolegend) for 30 min ...
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bioRxiv - Immunology 2023Quote: ... to block Fc receptors followed by staining with fluorochrome-conjugated anti–CD11b (1:200 dilution, BioLegend, no. 101226), anti–Ly6C (1:200 dilution ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated for 10 min at 4 °C with Fc receptor block (TruStain FcX, BioLegend, 1:10). Cells were washed and incubated in FACS buffer (1xPBS ...
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bioRxiv - Immunology 2024Quote: ... Cells were blocked for 15 min with 10 µL of human Fc receptor blocking solution (TruStain FcXTM; BioLegend). To minimize the interaction of polymeric dyes and improve discrete fluorochrome readout ...
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bioRxiv - Biochemistry 2023Quote: ... Fc-receptors were first neutralized by incubating cells on ice for 5-10 minutes with TruStain FcX (BioLegend) at 2 μg/mL in 100 μL of blocking buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... The pooled barcoded sample was stained for 10 minutes at room temperature with Fc receptor blocking solution (BioLegend) followed by 1h incubation with the antibody staining cocktail at room temprature ...
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bioRxiv - Neuroscience 2023Quote: ... FCS files were evaluated with the LEGENDplex™ Qognit 53790 Data Analysis Suite (Biolegend, San Diego, CA, USA). A five-parameter logistic curve-fitting method was used to calculate concentrations from Median Fluorescence Intensity values ...
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bioRxiv - Immunology 2023Quote: ... and unspecific binding of antibodies to Fc receptors was blocked by incubating the cells with TruStain FcXTM (Biolegend) diluted 1:50 in FACS buffer for 15 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Tissue samples being directly analysed ex vivo were Fc-blocked by incubation with 5μg/ml αCD16/32 (BioLegend) for 10 minutes prior to viability and surface staining ...
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bioRxiv - Cancer Biology 2022Quote: ... 2% FCS and 0.09% NaN3) and stained with fluorophore-conjugated antibodies (CD146-APC, SHM-57 or P1H12, BioLegend), (EPCAM-FITC ...
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bioRxiv - Cancer Biology 2023Quote: ... Resuspended cells were then pooled into a single tube and incubated with Tru-Stain Fc blocking solution (Biolegend) for 10 mins at RT ...
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bioRxiv - Immunology 2023Quote: ... Individual samples were then combined and stained with intracellular antibodies in CyTOF buffer containing Fc receptor blocker (BioLegend) overnight at 4C ...
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bioRxiv - Immunology 2023Quote: ... Cells were next treated with human 5µl/tube TruStain FcX™ (Fc receptor blocking solution, Cat# 422302, BioLegend) and incubated for 30 min at RT ...
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bioRxiv - Immunology 2023Quote: ... The cells were washed with cold PBS and PE-labeled anti-human IgG Fc secondary antibody (Biolegend, 41070) was added and incubated for 30 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... 107 cells per sample were then incubated with 10 μl of Fc-blocker (anti-CD16/CD32 antibody, Biolegend) for 30 min ...
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bioRxiv - Immunology 2023Quote: ... P008_108)52 for 1h at 4°C followed by PE-rat anti-human IgG Fc (1:200, Biolegend) for 45 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... then stained with the I-A/E-APC antibody (MHCII) (1:1,200) (eBioscience) and positively selected for I-A/E-positive cells using anti-APC Microbeads (Biolegend). The obtained final cell fraction consisted of total mTECs (CD45-EpCAM+Ly51-I-A/E+).
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bioRxiv - Biophysics 2020Quote: ... The membrane was probed with an anti-Aβ antibody targeted to residues 1-16 (1:1000, #E 610, Biolegend) and a secondary anti-mouse IgG HRP-conjugated antibody (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... anti-mouse mouse MHC H-2 hashtag antibodies (Biolegend TotalSeq™) were used to label separate FACS-purified subsets which were washed ...
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bioRxiv - Microbiology 2021Quote: ... mouse monoclonal α-GFP and/or mouse monoclonal α-RpoA (BioLegend) primary antibodies ...
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bioRxiv - Immunology 2021Quote: ... and mouse recombinant PE anti-human/mouse TLR2 (clone QA16A01; Biolegend) were used.
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bioRxiv - Immunology 2021Quote: ... LEGENDPlex assays (Mouse Proinflammatory Chemokine Panel and Mouse Inflammation Panel; BioLegend) were used ...
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bioRxiv - Immunology 2024Quote: ... into the mouse tail vein (5µg/25g mouse, Clone 1A8, Biolegend). 48 hours after the first FITC anti-mouse Ly-6G antibody injection ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse primary cells were treated with anti-mouse CD16/32 (Biolegend) and stained with fluorescent antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... a mouse biotinilated anti-mouse CD45 antibody (clone 30-F11, Biolegend) was used ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubation (VMAT2, 1:50, EB06558, Everest Biotech, Ramona, USA, HA-tag: 1:50, 682404, Biolegend, San Diego, USA) was performed for 60 min ...
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bioRxiv - Biophysics 2022Quote: ... Equivalent surface expression level was verified using an Alexa Fluor 488-conjugated anti-HA-tag mAb (clone 16B12, 1:1000 Biolegend). Flow cytometry data were analyzed using FlowJo V10 software.
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bioRxiv - Microbiology 2021Quote: ... Binding of His-tagged S1-RBD was detected with Alexa Fluor 488 anti-his tag antibody (clone J095G46, Biolegend, UK) 1:5000 in 25 μL PBST + 0.1% BSA per well ...
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bioRxiv - Immunology 2020Quote: Alexa fluor 488 or Alexa fluor 594 or FITC or Alexa fluor 648 labeled anti-His-tag (MBL or Biolegend),
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bioRxiv - Genomics 2021Quote: ... Sample from each donor is also stained with unique hash-tag barcoded antibody (Biolegend totalseq-C, cat# 394661, cat# 39466310). After two washes ...
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bioRxiv - Immunology 2021Quote: ... washed twice in buffer (PBS + 0.5% BSA) and stained with the anti-FLAG-phycoerythrin (PE) antibody (anti-DYKDDDDK Tag, BioLegend 637310) for confirmation of scFv expression and RSV-F protein conjugated with Alexa Fluor 647 (AF647 ...