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Citations for BioLegend :
851 - 900 of 5311 citations for Hepatitis E ORF2 protein mouse Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Blast cells (0.1-0.2 x 106) were washed and blocked with Human TruStain Fc blocker (BioLegend) for 15 minutes at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... 10 mg of protein G agarose beads were crosslinked to 5 μL of mouse anti-HA antibody (BioLegend, 901501, 1:1000, RRID: AB_2565006) using an effective concentration of 6.5 mg mL-1 DMP ...
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bioRxiv - Immunology 2019Quote: Antibodies used for FACS surface and intracellular staining were as follows: IA/E-PE (Biolegend, M5/114.15.2), Bst-2-biotin (in-house conjugated ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed twice with CSM before pooling and staining with E-Cadherin-AF647 (clone 67A4, Biolegend) and EpCAM-FITC (clone 9C4 ...
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bioRxiv - Immunology 2024Quote: ... and MHC Class II (MHCII) I-A/I-E (stains MMs, 1:100, clone MS/114.15.2, Biolegend) were used ...
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bioRxiv - Immunology 2022Quote: ... Proliferation was determined by labeling T cells with the Tag-IT Violet Proliferation Cell Tracking Dye (Biolegend, #425101), according to manufacturer instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Western blot analysis was performed using anti-HA.11 epitope tag antibody (BioLegend, 901501, 1:1000, RRID: AB_2565006), anti-FLAG (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Washed cells were stained with two fluorescently labeled epitope tag-specific antibodies (anti-FLAG PE, Biolegend, cat# 637309), anti-HA Alexa 647 ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were washed with PBS containing 1% FBS and incubated with APC anti-His Tag Antibody (BioLegend) for 20 minutes on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... transgenic T cells were labeled with Tag-it Violet Proliferation and Cell Tracking Dye (Cat No. 425101 Biolegend). The frequency of SMARTA cells that proliferated during culture was determined as described by Gett et al ...
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bioRxiv - Microbiology 2023Quote: ... gonorrhoeae (1 x 108 CFU) was incubated with 1.5 µl Tag-IT® Violet (TIV) (Biolegend, Catalog # 425101) in 100 µl 1X PBS + 5 mM MgSO4 ...
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bioRxiv - Immunology 2023Quote: ... or MCC:I-Ek (cognate) tethered pMHCII complexes were labeled with Tag-it Violet according to the manufacturer’s instructions (BioLegend). M12 cells and 58α-β- cells were then chilled on ice for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... for 15 minutes at 4°C followed by incubation with AntiHA.11 Epitope Tag Affinity Matrix (BioLegend 900801) for 4 hours at 4°C under constant rotation ...
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bioRxiv - Cancer Biology 2019Quote: ... and mouse TruStain FcX (anti-mouse CD16/32, Biolegend) and incubating at 4C for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: ... and AF700-conjugated mouse anti-mouse CD45.2 (104; BioLegend) along with eF780 viability dye (BioLegend ...
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bioRxiv - Microbiology 2022Quote: ... PE-Cy5-conjugated mouse anti-mouse NK1.1 (PK136; BioLegend), PE-Cy5.5-conjugated rat anti-mouse CD4 (RM4-5 ...
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bioRxiv - Immunology 2024Quote: ... mouse anti-mouse amyloid beta (Clone 6E10, Biolegend, 803004), and rabbit anti-mouse Iba1 (Fujifilm ...
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bioRxiv - Immunology 2023Quote: ... we cultured mouse T cells in mouse RP10 with recombinant mouse TGF-β1 (Biolegend Cat # 763104), rIL-12 ...
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bioRxiv - Cell Biology 2020Quote: 100µl bone marrow were blocked with 2µl Fc Receptor Blocking Solution (Human TruStain FcX™, (422301, Biolegend) for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5µg of TotalSeq™-A (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... then incubated with 0.5 ml of human TruStain FcX Fc receptor blocking solution (Biolegend, San Diego, CA) for 10 minutes.
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bioRxiv - Immunology 2021Quote: ... cells were washed twice in MACS buffer and incubated with PE–anti-human FC antibody (Biolegend, M1310G05) in MACS buffer for 30 min on ice ...
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bioRxiv - Neuroscience 2021Quote: ... centrifuged at 420 x g for 5 minutes and resuspended in anti-CD16/CD32 (Fc Block; Biolegend) diluted 1:50 in FACS buffer (PBS with 2% BSA and 1 mM EDTA ...
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bioRxiv - Immunology 2021Quote: ... The nonspecific binding of Fc receptors was blocked using anti-CD16/32 antibody (BioLegend, catalog no. 101302) on ice for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5μg of TotalSeq-A (Biolegend ...
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bioRxiv - Genomics 2022Quote: ... and staining was performed in the presence of Fc block (eBioscience/ThermoFisher, True-Stain Monocyte Blocker (Biolegend), and Brilliant Stain Buffer (BD Bioscience) ...
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bioRxiv - Cancer Biology 2022Quote: ... was used as the primary antibody and rat anti-human IgG Fc antibody conjugated to APC (BioLegend) was used as the secondary antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 1×106 cells were aliquoted in 5 ml tubes and incubated with Fc Blocking antibody (Biolegend). Antibodies used for immune profiling are detailed in Supplementary Table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... they were blocked in 1% BSA in PBS and Fc blocker (unlabeled anti-CD16/32, Biolegend 101302) for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7 ...
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bioRxiv - Immunology 2021Quote: ... Following a wash, cells were incubated (RT, 10 min) with Rhesus Fc Receptor Binding Polyclonal Antibody (Biolegend). Desired staining antibodies were added directly to this mixture at indicated dilutions (Supplemental Table 27 ...
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bioRxiv - Immunology 2022Quote: ... or plate-bound α-CD3ε (2 μg/mL) and plate-bound CD155-Fc (1 μg/mL; BioLegend) for four days ...
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bioRxiv - Developmental Biology 2022Quote: ... centrifuged and 500xg for 5 min and blocked with Fc block CD16/32 (Biolegend, #101302, 1:100), for 5-minutes on ice and stained with PECAM1-BV605 (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: ... 100,000–500,000 single cells of breast tumours were suspended in 100μl staining buffer (2% BSA, 0.01% Tween in PBS) before adding 10µl Fc-blocking reagent (FcX, BioLegend). and incubating during 10 min on ice ...
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bioRxiv - Immunology 2022Quote: ... and then incubated with Fc block TruStain FcX (CD16/32) to block nonspecific FcR binding (#422302, Biolegend). To detect MHC-I and MHC-II ...
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bioRxiv - Microbiology 2022Quote: ... 1×106 cells were plated and then incubated with Fc block (Biolegend; cat#101320; 1:100 dilution) and surface stained for 20 min at 4°C in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... Fc receptors were then blocked with an anti-CD16/CD32 antibody (BioLegend, San Diego, CA, U.S.A., #553141). Cells were washed and then incubated with conjugated antibodies (FITC-CX3CR1 ...
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bioRxiv - Immunology 2024Quote: ... Cells that were used in CITE-seq experiments were blocked with TruStain Fc block (BioLegend; Cat. 422301) for 30 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.01% NaN3 in 1X PBS) and blocked with Fc receptor antibody specific for FcyR III/II (Biolegend TruStain FcX PLUS anti-mouse CD16/32 ...
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bioRxiv - Cancer Biology 2023Quote: ... Fc-block was performed by incubating cells in a dilution of Human TruStain FcX (#422302, BioLegend, USA) and mouse TruStain FcX (#101320 ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in semi-facs buffer in the presence of Fc receptor blocking antibody (BioLegend, 101302) and stained with antibodies at a 1:200 dilution with the myeloid or lymphoid antibody cocktails ...
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bioRxiv - Genomics 2023Quote: ... The PBMC pellet was resuspended and cells were stained with Human TruStain FcX Fc Blocking Reagent (BioLegend) for 10 min at 4°C and then with fixable viability dye DAPI (Merck) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated with 2 μg/mL of phycoerythrin (PE) anti-human IgG Fc (Clone M1310G05, BioLegend). Cells were washed once ...
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bioRxiv - Immunology 2023Quote: ... the cells were blocked in 1% BSA in PBS and Fc blocker (anti-CD16/32, Biolegend 101302) for 10 min at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... CAR-M cells was prepared and the Fc receptor was blocked with anti-moCD16/32 antibody (BioLegend) for 10 minutes at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... cells were stained with live dead cell staining dye NIR and then incubated with Fc block (BioLegend). Cells were further stained with the following fluorochrome-conjugated antibodies ...
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bioRxiv - Immunology 2022Quote: ... HIV-infected cells were previously incubated with 10 μg/ml of anti-human HLA-E (clone 3D12, Biolegend) antibody for 20 mins at RT ...
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bioRxiv - Immunology 2020Quote: ... dil. 1:400), MHC class II (I-A/I-E) (M5/114.15.2; FITC, PerCP, dil. 1:800) (BioLegend). Data analysis was performed using FlowJo version 10 (TreeStar ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-Phospho-c-Jun (Ser73) (D47G9) (Cell Signalling, #3270) and Ly-6A/E (Sca-1) (Biolegend, cat#108101). Appropriate Alexa Fluor labelled secondary antibodies (ThermoFischer Scientific ...
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bioRxiv - Immunology 2022Quote: ... Cell surface MHC class II and CD86 on BMDCs were stained with anti-I-A/I-E-PerCP (clone M5/114.15.2, BioLegend) and anti-CD86-PE (clone GL-1 ...
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bioRxiv - Immunology 2024Quote: ... Surface MHC class II and CD86 on BMDCs were stained with anti-I-A/I-E-PerCP (clone M5/114.15.2, BioLegend) and anti-CD86-PE (clone GL-1 ...