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Citations for BioLegend :
901 - 950 of 1002 citations for 6 fluoro 5 nitroquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref. C15410056; anti-HA.11 BioLegend ref ...
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bioRxiv - Immunology 2024Quote: ... FL or bone marrow HSPC were cultured on OP9-DL1 cells in the presence of 1 ng/mL IL-7 and 5 ng/mL FLT3L (BioLegend) as previously described 70 ...
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bioRxiv - Immunology 2024Quote: ... Mice CD4+ T cells (4x105/well in 96-well plate) were activated with plate-coated αCD3 (5 µg/ml, Biolegend) and αCD28 (10 µg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were immunolabeled in blocking solution for 1 hour at RT with antibodies against the following proteins: ALIX at 5 μg/ml (mouse; no. 634502, Biolegend); IST1 at 0.4 μg/ml (rabbit ...
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bioRxiv - Bioengineering 2024Quote: T-cells were cultured in RPMI-1640 media supplemented with 5 µg/mL brefeldin A (BioLegend, San Diego, CA, USA). After 24 hours of cultivation ...
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bioRxiv - Immunology 2024Quote: ... for 40 min on ice or with biotinylated anti-MR1 antibody 26.5 (BioLegend, custom conjugation, used at 5 μg/ml) for 40 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... 106 of the CTV labeled T cells were stimulated in vitro for 3 or 5 days in cultures containing 100ng/ml IL-15 (BioLegend) or a combination of 100ng/ml IL-7 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... isolated naïve splenic B cells were cultured at a density of 0,5 x 106 cells/ml in the presence of 25 μg/ml LPS and 5 ng/ml IL-4 and stained with anti-CD138 (BioLegend). B cell proliferation was assessed by CellTrace Violet (Thermofisher ...
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bioRxiv - Immunology 2022Quote: ... and SCT Dd or SCT Dd-CD4 cell surface expression was regularly assessed by flow cytometry (clone 34-5-85 and 34-2-12, Abcam and Biolegend, respectively). Transfectants were FACS-sorted (FACSDiva and FACSARIAIIIU cell sorter ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were first blocked with 5% BSA for 30 min and stained with Alexa Fluor® 647 anti-human CD161 antibody (clone HP-3G10; BioLegend) at 10 μg/ml in blocking solution for 60 min ...
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bioRxiv - Molecular Biology 2020Quote: Freshly isolated CD3+ T cells were labeled using CFSE working solution (5 μM) using the CFSE Division Tracker Kit (Biolegend, USA). T cells were incubated for 20 min at 37 ◻ and quenched staining with culture medium ...
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bioRxiv - Systems Biology 2019Quote: ... blocked in TBST buffer supplemented with 5% bovine serum albumin (BSA) and incubated overnight with primary anti-XBP1s antibody (Biolegend Poly6195) at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... or double knockout cells were harvested and washed with PBS containing 1% bovine serum albumin (BSA) and incubated with 5 μL of PE-conjugated EphA2 antibody (Biolegend, SHM16) in 50 μL PBS containing 1% BSA for 30 minutes at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Membrane was blocked in 5% milk followed by probing with either α-HA Mouse Monoclonal (Catalogue #HA.11; Biolegend, SanDiego, CA) or α-Rsp5 Rabbit Polyclonal [84] antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... resuspended in 100 µl of FACS buffer and incubated in 5 µl of Human TruStain FcX™ (Fc receptor blocking solution, BioLegend) for 10 minutes at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples that were stained for cell surface staining only were incubated for 5 min on ice in 7-AAD (Biolegend, #420404). Samples that were stained for intracellular markers were fixed and permeabilized using the eBioscience™ FoxP3/Transcription Factor Staining Buffer Set (Thermofisher ...
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bioRxiv - Microbiology 2020Quote: ... 5 × 105 cells/mL of CD14+ enriched cells were stimulated using recombinant human (rh) GM-CSF and rhIL-4 (BioLegend, USA) at a concentration of each 500 IU/mL in RPMI1640 media supplemented with 10% FCS at 37°C in 5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: ... The suspensions were then spun at 300 x g for 5 minutes and resuspended in red blood cell (RBC) lysis buffer (Biolegend, 420302) for 5 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were harvested for 5 min at 400g at RT and stained with Pacific Blue™ anti-human Ki-67 Antibody (Biolegend) according to eBioscience(™ ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were centrifuged at 1800 rpm for 5 mins and then incubated in 2 μg/mL solution of anti-CD11b antibody (Biolegend 101257) and anti-GR1 antibody (Biolegend 108422 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The membrane was next added to 5% nonfat milk in TBST containing horseradish peroxidase-conjugate goat anti-mouse IgG1 secondary antibodies (Biolegend 405306) diluted at 1:5000 and incubated on a horizontal rocker for 60 minutes ...
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bioRxiv - Immunology 2020Quote: ... once a week every week with IL-15 Receptor alpha Fc Chimera (R&D; 2.5μg/mouse) and recombinant human IL-15 (Biolegend, 5 μg/mouse). For immune blockade of PD-1 ...
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bioRxiv - Immunology 2020Quote: ... cells were pelleted for 5 minutes at 500xg and resuspended in 50 µL Intracellular Staining Perm Wash Buffer supplemented with 5 µL of TruStain FcX (Biolegend, 422302) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... The frozen tissue sections were treated with blocking solution (5% normal donkey serum in PBS) and then stained with FITC-conjugated anti-Ly6G antibody (BioLegend, 1A8). Nuclei were stained by DAPI ...
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bioRxiv - Immunology 2020Quote: ... were resuspended in 100μl protein blocking solution with 5 μl fluorescent conjugated antibodies, CD8 (Biolegend Cat# 100714, CD45 (Biolegend Cat# 103130), CD68 (Biolegend Cat# 137004) ...
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bioRxiv - Microbiology 2022Quote: ... bacteria were labeled with 5 µg ml−1 carboxylfluorescein diacetate succinimidyl ester (CFSE) or Tag-IT Violet™ proliferation and cell tracking dye (Biolegend) in PBS containing 5 mM MgSO4 ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were stimulated in presence of self-made human IL-2 on α-CD3 (5 µg/ml) (145-2C11, BioLegend), α-CD28 (5 µg/ml ...
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bioRxiv - Immunology 2022Quote: ... single-cell spleen suspensions from C57BL/6J mice were cultured at 5 million cells/ mL in complete RPMI with 1 μg/mL anti-CD3 (BioLegend, #100340) and 1 μg/mL anti-CD28 (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated in primary antibodies diluted in 5% skim milk in TBST: mouse anti-brevican (1:1000, BioLegend, cat# 820101), sheep anti-neurocan (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... samples were pre-incubated for 5 minutes at 4°C with TruStain FcX™ (anti-mouse CD16/32) Fc block (clone 93; BioLegend). Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Then the live microglial cells were incubated with PBS supplemented with 5% BSA + HLA-DR mouse monoclonal antibodies pre-conjugated with FITC (1:100, BioLegend, Inc.) + Hoechst 33342 (1:5000 ...
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bioRxiv - Synthetic Biology 2022Quote: ... The membrane was incubated with 5% nonfat milk in TBST for 1 hour followed by the addition of primary antibodies (BioLegend, 652505). After 1 hour of the primary antibody incubation ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated with their respective primary antibodies diluted in 5% skim milk in PBST at 4 °C overnight: purified mouse anti-βIII-tubulin (1:1,000) (BioLegend, cat. 801202), monoclonal mouse anti-GAPDH (1:50,000 ...
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bioRxiv - Cancer Biology 2022Quote: ... spun at 700 g for 3 minutes at 4°C and washed in MACS Buffer 3× before being incubated with the following antibodies: APC-conjugated rat anti-CD3 (5 μL/10×106 cells; Biolegend, 100408), PE-conjugated rat anti-CD8b (2.5 μL/10×106 cells ...
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bioRxiv - Synthetic Biology 2022Quote: ... The membrane was incubated with 5% nonfat milk in TBST for 1 hour followed by the addition of primary antibodies (BioLegend, 652505). After 1 hour of the primary antibody incubation ...
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bioRxiv - Biophysics 2023Quote: ... The gel substrate for T cells had a Young’s modulus of 1.2 kPa and was coated with 5 μg/mL each of anti-CD3 (OKT3; 317326; BioLegend, CA, USA) and anti-CD28 (CD28.6 ...
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bioRxiv - Cancer Biology 2023Quote: ... centrifuged and 500,000 cells were stained in 200 µl 1x PBS plus 0.5 µg of indicated antibody for 30 min in the dark: APC anti-mouse CD274 (B7-H1, PD-L1, Biolegend #124311) or appropriate IgG isotype ...
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bioRxiv - Bioengineering 2023Quote: ... Successful blocking was confirmed by staining with anti-human CD44 antibody conjugated with Alexa Fluor 594 (clone: C44 Mab-5; Biolegend, USA) and quantified using the Cytek Aurora flow cytometer (4L 16V-14B-10YG-8R ...
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bioRxiv - Bioengineering 2023Quote: THP-1 cells were incubated with 2 µg/ml of anti-CD44 antibodies (clone: C44Mab-5; Biolegend, San Diego, CA, USA) for 20 minutes under standard cell culture conditions (95% humidity and 5% CO2 at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then blocked in 5% NDS in TBS for 1 hour before being incubated with 1:1000 6E10 (BioLegend 803004) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Each well was blocked using 50 μL of FACS buffer (PBS + 5% FBS) + 2.5 μL Fc receptor (BioLegend, Cat No. 422302) block per well and then 150 μL FACS buffer was added and spun down ...
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bioRxiv - Molecular Biology 2023Quote: ... Collected cell populations were immediately seeded in tissue culture at a concentration of 2x105 cells per 1.9 cm2 in DMEM containing 5% FBS and 10 ng/mL TGFβ (Biolegend Cat 763102) or 500 nM Prostaglandin F (Cayman Chemical Cat 16010).
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bioRxiv - Neuroscience 2023Quote: ... The cell pellets were harvested and resuspended in 5 mL of red blood cell (RBC) lysis buffer (BioLegend, San Diego, CA) for 5 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Cells were pelleted by centrifugation (2,000 rpm 5 min) and then treated with 1 mL 1X red blood cell (RBC) Lysis Buffer (Biolegend; cat# NC9067514) to lyse red blood cells ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed three times (300g, 5 min, 4⁰C) in cell staining buffer and kept in FluoroFix™ Buffer (422101, Biolegend) before run in a BD FACSymphony™ A5 Cell Analyzer (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... cells were washed three times (300g, 5 min, 4⁰C) in cell staining buffer and kept in FluoroFix™ Buffer (422101, Biolegend) prior to run in a BD FACSymphony™ A5 Cell Analyzer (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... followed by 5 µL/well of the antibody cocktail for extracellular staining: anti-CD3-BV421 (clone 17A2) (BioLegend, SanDiego, CA, USA) (1:200) ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 1 x 106 cells were resuspended in 10 uL of PBS supplemented with 2% FBS and 2 mM EDTA (hereafter named ‘FACS buffer’) together with 5 uL of TruStain FcX (BioLegend, 422301). Cells were incubated with the FcX block for 10 minutes at RT ...
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bioRxiv - Bioengineering 2024Quote: Resting T-cells were cultured in RPMI-1640 media supplemented with 5 µg/mL brefeldin A (BioLegend, San Diego, CA, USA). After 24 hours of cultivation ...
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bioRxiv - Genomics 2020Quote: ... The cells were centrifuged at 300xg for 5 minutes at room temperature and resuspended in 85 ul of staining buffer and incubated with 5 ul of Human TruStain FcX(Biolegend cat: 422301) for 10 minutes on ice in 5 ml FACS tubes ...