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Citations for BioLegend :
551 - 600 of 1002 citations for 6 fluoro 5 nitroquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Serum samples were diluted 1:5 in ELISA diluent buffer (Biolegend).
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bioRxiv - Immunology 2023Quote: ... in the presence of brefeldin A (5 µg ml-1; Biolegend) for 4 h ...
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bioRxiv - Immunology 2024Quote: T cells were stained before seeding with 5 μM CFSE (BioLegend) for 20 min at 37°C according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Antibodies for flow cytometry were PerCPCy5-5-CD15 (HI98, Biolegend #301922), AF488-CD11b (ICRF44 ...
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bioRxiv - Neuroscience 2023Quote: ... and 5 µg AlexaFluor647 conjugated to anti-CD8 antibody (100724; Biolegend) 40 minutes before imaging ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-Keratin 5 (PRB-160P, BioLegend, Dedham, MA, USA, 1:500); anti-CC10 H-75 (sc-25554 ...
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bioRxiv - Cancer Biology 2021Quote: NK cells were isolated from C57BL/6 mice (male and female) with the MojoSort mouse NK cell isolation kit (BioLegend) (purity >85 % based on the flow cytometric analysis with NK1.1 and CD49b antibodies) ...
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bioRxiv - Cancer Biology 2021Quote: ... The following anti-mouse antibodies we used for surface staining: Alexa Fluor 647-labeled H-2Kb/H-2Db – MHC Class I (clone 28-8-6, Biolegend); PE-labeled I-A/I-E – MHC Class II (Clone M5/114.15.2 ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL were then stained with 2.5 μL FITC anti-human CD19 antibody (BioLegend, 392508), 2.5 μL FITC anti-human CD20 antibody (BioLegend ...
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bioRxiv - Neuroscience 2019Quote: ... slides were incubated for 1.5 hrs at RT with a cocktail of secondary fluorescence-conjugated antibody Alexa488 and DAPI (4’,6-diaidino-2-phenylindole, dilactate; BioLegend) to visualize nuclei ...
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bioRxiv - Immunology 2020Quote: ... Phenotypes of B cells before and after 6 days of culture were determined by flow cytometric analysis using mouse monoclonal antibody clones: CD19 PECy7 HIB19 (Biolegend), CD27 BV421 O323 (Biolegend) ...
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bioRxiv - Genomics 2020Quote: ... 1.25 × 10^6 PBMCs were spun down and resuspended in 25 μl CITEseq antibody cocktail (TotalSeq™-A Antibodies, BioLegend) containing among others one hashing antibody per each sample ...
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bioRxiv - Microbiology 2021Quote: ... prior to staining cells in 100 µL perm/wash with 0.5 µL APC anti-human IL-6 antibody (Cat# 501112, Biolegend®) overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... with recombinant IL-15/IL-15RA complex (100 ng/ml; Thermo Fischer, Waltham, MA) or recombinant mouse IL-6 (50 ng/ml, BioLegend), TGF- β1 (1ng/mL) ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were then tagged with mouse Total-Seq anti-mouse hashtags C0306 (Brafi: Hashtag 6) and C0307 (Brafi + C-Me: Hashtag 7) (BioLegend) according to manufacturers’ protocols.
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bioRxiv - Developmental Biology 2023Quote: ... NPCs were we pelleted and washed once with AutoMACS buffer before resuspending AutoMACS with in DAPI (dead cell dye DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate, 422801, BioLegend) and DRAQ5 (DRAQ™ Live cell dye ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... CD3+ T cells were purified from C57BL/6 spleen cells with MojoSort mouse CD3 T cell isolation kit (Biolegend, USA), while CD8+T cells were isolated by adding biotin-labeled anti-CD4 antibody into MojoSort mouse CD3 T cell isolation kit ...
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bioRxiv - Physiology 2023Quote: ... IL-6 and IL-10 in the homogenates were measured using LEGENDplexTM Mouse Custom Panel (BioLegend, San Diego, CA, USA). Assay was performed in the Attune NxT flow cytometer (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... from uninfected or MCMV-infected WT C57BL/6 mice were sorted as described and stained with barcoded antibodies (Total-Seq B, BioLegend). After hash-staining ...
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bioRxiv - Immunology 2024Quote: ... The sections were then rinsed in a washing solution twice before nuclei were stained with 4’,6-diamidino-2-phenylidole (DAPI; BioLegend). The sections were rinsed with washing solution one final time and then mounted on slides for examination under an Olympus FV1000 laser-scanning 4-channel confocal microscope (Olympus).
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bioRxiv - Immunology 2024Quote: ... Flow cytometry analysis of CTLA4-mediated trans-endocytosis was performed as previously described [6] using the True-NuclearTM Transcription Factor Buffer Set (BioLegend) and the following intracellular antibody panel ...
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bioRxiv - Cancer Biology 2019Quote: ... at concentration 2×10^6/ml in IMDM containing 10% FBS and 100U/ml of recombinant mouse IL-2 (BioLegend, CA). Cells were cultured for up to 7 days and used for functional experiments in vitro and in vivo.
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bioRxiv - Microbiology 2021Quote: ... and resuspended in RPMI with 10% FBS medium and were stimulated for 6 h with a cell activation cocktail containing PMA and ionomycin (Biolegend, # 423302) in the presence of protein transport inhibitor ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL solution were then stained with 3 μL FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μL Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Immunology 2020Quote: Supernatants of stimulated T cells were harvested on day 6 of culture and analyzed using the LEGENDplex™ human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Developmental Biology 2021Quote: ... Surface staining was done by adding the flow cytometry antibody cocktail outlined in Extended Data Table 6 containing F4/80 (BioLegend, 123120), CD11b (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were induced to differentiate by culturing in the absence of β-Estradiol for 6 days before cells were stained for CD11b (Biolegend) and analyzed on the LSRII flow cytometer (BD Biosciences) ...
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bioRxiv - Microbiology 2023Quote: ... and subsequently with biotinylated α- CADM1 antibody (MBL, Cat. No. CM004-6) and streptavidin-conjugated secondary antibody (BioLegend, Cat. No. 405226). Cells were resuspended in 4◦C PBS 2% FCS before cell sorting.
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bioRxiv - Cancer Biology 2023Quote: For inflammatory activation colon fibroblasts were plated in 6-wells and after 24 hours treated with 1 ng/ml murine recombinant IL-1α (Biolegend, 575002) in PBS for the indicated time periods ...
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bioRxiv - Cancer Biology 2023Quote: Plasma Ig were analysed using the LEGENDplex Mouse Immunoglobulin Isotyping panel (6-plex) by flow cytometry (Cat #740493, BioLegend, CA, USA). Plasma sample and standards was prepared using assay buffer as per the manufacturer’s instruction ...
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bioRxiv - Physiology 2023Quote: The levels of TNF-α and IL-6 in the serum and the supernatant of the crushed muscle tissues were measured using a commercial kit (mouse TNF-α and IL-6 ELISA max standard set, BioLegend). The values of TA and GA muscles were revised to the protein concentration measured by BCA kit and expressed as pg/mg.
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bioRxiv - Immunology 2023Quote: ... CD3+ T cells were isolated from spleens of 6-to-8-week-old OT-II mice by MojoSort mouse CD3+ T cell isolation kit (Biolegend 480024). Stimulated FLS were washed with PBS and co-cultured with CD3+ T cells at a concentration of 5-10×105 cells/mL in 10% RPMI for 5 days with either the cognate OVA323-339 antigen (2μg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... Splenocytes from naive or tumor-bearing AST;Cre-ERT2 mice were mixed with 2×106 C57BL/6 splenocytes and incubated in RPMI-10 for 4 hours at 37°C in the presence of brefeldin A (Biolegend 420601) and TAG peptide (SAINNYAQKL [0.5 μM] ...
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used: CD4 (Clone RM4-5, BioLegend, Cat 100527), GMCSF (Clone MP1-22E9 ...
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bioRxiv - Immunology 2019Quote: ... cells were stained with biotin-conjugated anti-CD2 (clone RM2-5, BioLegend) antibody and negative selection was performed by using an EasySep Biotin Selection Kit (Stemcell Technologies ...
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bioRxiv - Immunology 2020Quote: ... Cells were then incubated with 5 μL APC Annexin V (Biolegend: 640920) and 10 μL PI solution (Biolegend ...
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bioRxiv - Bioengineering 2022Quote: ... 5 μL of 25 μg/mL anti-CD4 PE (Biolegend, Catalog: 344606) and 5 μL of 100 μg/mL anti-CD8 Alexa Fluor 488 (Biolegend ...
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bioRxiv - Bioengineering 2022Quote: ... and 5 μg/mL of CD40 antibody ligand (clone: HM40-3; BioLegend).
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bioRxiv - Immunology 2022Quote: ... Cells were treated with 5 µg/mL of Brefeldin A (BioLegend, 420601) and allowed to incubate for 6 hours in a 5% CO2 ...
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bioRxiv - Immunology 2019Quote: ... TNFα was blocked with 5 µg/mL anti-TNFα antibody (Mab11, BioLegend); IgG1 isotype control (MOPC-21 ...
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bioRxiv - Cancer Biology 2019Quote: ... Human CD96 blocking antibody (5 μg/ml) or isotype control (Biolegend, USA) was included in some experiments.
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bioRxiv - Genetics 2020Quote: ... We stimulated Jurkat cells with 5 μg/ml anti-CD3 (Biolegend-317315) and 100 ng/ml phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Neuroscience 2021Quote: ... and Rat IgG2a,k AF647 Isotype Control (RTK2758, 5 μg/mL, Biolegend).
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bioRxiv - Immunology 2021Quote: ... in the presence of plate- bound anti-CD3 (5 μg/mL, Biolegend), CpG (10 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... CD4 (RM4-5; BV 711) and PD-1 (J43; FITC; all BioLegend). After fixation and permeabilization (Foxp3 Transcription Factor Staining Buffer Set ...
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bioRxiv - Immunology 2022Quote: ... and blocked with 5 μg/mL anti-CD16/32 (clone 93; Biolegend) and heat inactivated normal mouse serum (1:10 ...
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bioRxiv - Immunology 2022Quote: ... single cell suspensions were incubated for 5 hours in 2μM monensin (BioLegend) to inhibit intracellular protein trafficking ...
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bioRxiv - Cancer Biology 2022Quote: ... and stimulated with 5 ug/mL Ultra-LEAF anti-human CD3 (Biolegend) and 2.5 ug/mL Ultra-LEAF anti-human CD28 (Biolegend) ...
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bioRxiv - Immunology 2023Quote: ... wells were pre-coated with 5 μg/mL ICAM-1 (Biolegend, #553006) for 2-3 hours at 37°C before being emptied for seeding ...
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bioRxiv - Microbiology 2023Quote: ... CD4 (mouse, FITC fluorochrome, clone RM4-5, BioLegend 100510, 1:200 dilution), CD8a (mouse ...