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Citations for BioLegend :
901 - 950 of 8202 citations for 4 2 Hydroxyimino 2 4 methoxy phenyl ethyl piperazine 1 carboxylic acid tert butyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... cells were re-stimulated for 4 hr with PMA/Ionomycin and Brefeldin A (Biolegend), according to manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... After 4 days of co-culture cells were collected stained for CD8-PECy5 (BioLegend) and assessed for cell proliferation with flow cytometry ...
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bioRxiv - Immunology 2024Quote: ... The PE-conjugated antibody for murine TLR-4 antibody (145404) was purchased from BioLegend. anti-TNFR1 (Armenian hamster IgG ...
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bioRxiv - Immunology 2023Quote: ... Cells were placed in 4 ml of 1X annexin binding buffer (Biolegend, cat#: 422201) with biotinylated annexin V (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... and cultured for 4 days prior to staining with anti-CD8-APC (SK1, BioLegend) and proliferation measured by flow cytometry ...
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bioRxiv - Cancer Biology 2022Quote: ... blocked with TruStain FcX (α-mouse CD16/32; 10 min, 4°C; #101320, BioLegend) and stained (20 min ...
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bioRxiv - Immunology 2023Quote: ... Activation was measured by the following markers: CD137 APC (4:100; Biolegend Cat# 309810) and OX40 PE-Cy7 (2:100 ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4°C with the anti-mouse IFNγ AN18 antibody (Biolegend) at 2 μg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... HA-TAP-Gb1 and endogenous Gb1-4 were detected with anti-HA (16B112, BioLegend) and anti-Gb1-4 (sc-378 ...
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bioRxiv - Immunology 2023Quote: ... tissue sections were stained O/N 4°C with either anti-CD11c (N418, Biolegend) and anti-CD63 (NVG-2 ...
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bioRxiv - Biophysics 2024Quote: ... blots were incubated overnight at 4°C with anti-ASIC1 primary antibody (BioLegend #833501) at a 1:1000 dilution ...
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bioRxiv - Immunology 2024Quote: ... Capture and biotin conjugated detection anti-mouse IL-4 antibodies were purchased from BioLegend. Anti-mouse IL-13 antibody pair were purchased from Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: WT mice were retro-orbitally injected with 4 μg CD45-FITC (Cat. #103108; Biolegend). Mice were then allowed to recover ...
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bioRxiv - Cancer Biology 2024Quote: ... for 4 minutes before stopping the reaction with 25 µl of Stop Solution (BioLegend). We analyzed the plates on an Infinite m200 microplate reader at a wavelength of 450 nm (Tecan) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then stained in FC buffer (2% FCS in PBS) with anti-CD3-APC conjugated antibodies (1:80; BioLegend) and LIVE/DEAD Fixable Near-IR Dead Cell Stain (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... CD18 (clone CBR LFA-1/2, 366310), ITGB7 (clone FIB504, 121006), and CD68 (clone Y1/82A, 333814) were acquired from Biolegend.
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bioRxiv - Bioengineering 2022Quote: ... Splenic cells at 1 × 106 were co-cultured with target cells at 1:2 effector to target ratio in the presence of anti-CD107a (Biolegend), brefeldin A and monensin and a serial dilutions (6x ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained at 4°C for 30 min in Hanks Balanced Salt Solution-2% FBS with the following markers: anti-THY1 AF700 1:100 (Biolegend), anti-PDGFRalpha PE 1:100 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... for 30’ at RT in PBS containing 2% FCS and 1 mM EDTA prior to secondary staining with streptavidin-BrilliantViolet 510 (Biolegend) for 15’ on ice ...
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bioRxiv - Immunology 2021Quote: ... The antigenicity and the immunogenicity of the purified recombinant spike protein was validated (Supplementary Fig 1) against verified available recombinant spike protein (BEI Resources, NR52397) and anti-SARS-CoV-2 spike antibody clone A20085C (BioLegend).
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bioRxiv - Immunology 2021Quote: ... or total combined splenocytes plus bone marrow from WT or c-Rel-/- mice were stimulated for 48 hours in complete RPMI containing 10% fetal bovine serum (FBS) and 1% pen strep with either rmIL-2 (1000 U/ mL; BioLegend), rmIL-15 (100 ng/mL ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were cultured alone or co-cultured with either of the B-cell populations at a ratio of 3:1 for 60 h at 37°C and 5% CO2 on anti-CD3 (2 μg/mL) (ULTRA-LEAF Biolegend) coated flat bottomed 384 CellCarrier Ultra microplates (PerkinElmer ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with FACS staining buffer (PBS containing 2% fetal bovine serum [FBS] and 1 mM EDTA) and incubated with an Fc receptor blocker (TrueStain fcX; BioLegend) for 5 minutes at 4℃ ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mM EDTA in PBS) and incubated with Fc-blocking reagent (1:200, TruStain FcX, anti-mouse CD16/32, BioLegend). After washing ...
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bioRxiv - Immunology 2024Quote: ... were cultured for 3 days in 96-well U bottom plates coated with 2 μg/mL anti-CD3 (eBioscience) and 1 μg/mL anti-CD28 (eBioscience) supplemented with 10ng/mL of recombinant mIL-2 (Biolegend) to allow Treg survival ...
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bioRxiv - Developmental Biology 2024Quote: ... and the resulting cDNA was amplified using as PCR oligos the partial TruSeq read 1 adaptor (provided in the 10x kit) and an additive primer (a partial TruSeq read 2, sequence provided by Biolegend and synthesized by Integrated DNA Technologies) ...
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bioRxiv - Immunology 2024Quote: ... plates were coated per well with 100 µL of anti-IgE capture antibody (2 µg/mL in PBS, clone RME-1, BioLegend) overnight at 4⁰ C ...
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bioRxiv - Immunology 2022Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...
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bioRxiv - Immunology 2023Quote: Supernatants from stimulated T cells were harvested on day 2 and day 6 depending on peak of cytokine production and analyzed using the LEGENDplexTM human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then diluted with 50 μL PBS + 2% FBS and FITC anti-CD45 (BioLegend, 304005; 1:1,000 final dilution) and incubated for 20 min on ice in the dark ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Cells were centrifuged at 500× g at 4 °C and resuspended in 1/3 strength artificial seawater containing 2 μg/mL calcein AM (Enzo) and 100 nM sytox blue (BioLegend) to monitor viability ...
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bioRxiv - Immunology 2023Quote: ... 0.5-1×106 bone marrow cells were stained with the following surface antibodies (2 panels) against: CD14 (Biolegend, M5E2, AF700), HLA-DR (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: ... Surface staining of the Macrophage panel consisted of the following primary antibodies diluted in FACS buffer (2% FBS in DPBS) with anti-mouse CD45-Brilliant Violet 650 (1:200, 103151, Biolegend), anti-mouse F4/80-Brilliant Violet 785 (1:200 ...
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bioRxiv - Microbiology 2024Quote: ... LAMP-1 was stained as described previously for LAMP-2 but using a rat-IgG anti-mouse LAMP-1 and anti-rat-IgG-AlexaFluor647 (Biolegend) [56] ...
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bioRxiv - Immunology 2024Quote: ... and fluorochrome-conjugated anti-CD86 and anti-CD32 antibodies in FACS buffer (1%BSA and 2 mM EDTA in PBS) supplemented with True Stain Monocyte Blocker solution (Biolegend) to prevent aspecific binding of fluorochrome-conjugated antibodies to myeloid cells ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were washed with PBS containing 2 mM EDTA and stained with antibodies against CD59 (1:100, anti-human CD59 PE, Biolegend) and C3c (1:25 ...
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bioRxiv - Cancer Biology 2021Quote: ... and Alexa Fluor 647-conjugated BCL-2 (BioLegend Cat# 633510, RRID:AB_2274702). PE-Cy7-conjugated Streptavidin was used to detect biotinylated CD49d (9C10).
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bioRxiv - Immunology 2021Quote: ... anti-mouse mouse MHC H-2 hashtag antibodies (Biolegend TotalSeq™) were used to label separate FACS-purified subsets which were washed ...
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bioRxiv - Immunology 2021Quote: ... The antibody against Bcl-2 (BCL/10C4) was purchased from Biolegend. The antibodies against TCF1 (C63D9 ...
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bioRxiv - Bioengineering 2021Quote: ... and APC anti-human CD46 (clone TRA-2-10, Biolegend 352405). Antibody staining was performed for 20 minutes on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... washed with cold 2 ml staining buffer (BioLegend Inc, catalog # 420201) and resuspended in cold Annexin V binding buffer at 1×106 cells per ml ...
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bioRxiv - Immunology 2022Quote: ... PMN were stained with 2 μl of Zombie Aqua dye (BioLegend) diluted 1:100 for 15 min at RT ...
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bioRxiv - Immunology 2022Quote: ... AM were imaged PE-Siglec F (2 µg, clone S17007L; BioLegend) administered intravenously 30 mins after engraftment ...
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bioRxiv - Immunology 2022Quote: ... APC-anti human 2B4/CD244 (2-69; BioLegend, San Diego, CA); or with the isotype control APC mouse IgG1 (MOPC-21 ...
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bioRxiv - Immunology 2022Quote: ... anti-CD138-PE (281-2) and PE/Cy7- Streptavidin from Biolegend, and anti-FCRL5-Alexa488 and anti-FCRL5-APC (Polyclonal ...
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bioRxiv - Bioengineering 2020Quote: All reagents for detection of IL-2 were purchased from Biolegend. Supernatants were collected after 16 hours of coculture of 5MCAR 58α−β− cells and M12 target cells ...
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bioRxiv - Immunology 2020Quote: ... CD19 Pacific Blue (clone H1B19, BioLegend cat#302224, 2:100 dilution); CD3 AF700 (clone OKT3 ...
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bioRxiv - Immunology 2020Quote: ... CD4 PerCP-Cy5.5 (clone OKT4, BioLegend, cat# 317428, 2:100 dilution); CD127 AF488 (clone A019D5 ...
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bioRxiv - Immunology 2021Quote: ... or 5 μg/ml anti-human TRAIL antibody (RIK-2, Biolegend). After 2 h of preincubation effector cells were added to target cells.