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Citations for BioLegend :
801 - 850 of 8202 citations for 4 2 Hydroxyimino 2 4 methoxy phenyl ethyl piperazine 1 carboxylic acid tert butyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... then for 1 hour at 4°C in the dark with a 1:1 mix of rat anti-mouse CD41 (labelled with BV421) (BioLegend – 133911; 0.25 µg per million cells) and rat anti-mouse CD31 (labelled with AF647 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were applied overnight at 4°C and included SMI31 (Biolegend, 801601), IBA1 (Wako ...
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bioRxiv - Immunology 2021Quote: ... MDM were suspended in 100μL of 4°C annexin V binding buffer (BioLegend) and the two pHrodo loaded sets were stained with 2μL of FITC Annexin V (BioLegend) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then fixed in 4% paraformaldehyde and permeabilized using Permeabilization buffer (Biolegend). Intracellular proteins were stained with antibodies against TNF (MP6-XT22 ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were incubated overnight at 4°C in primary antibody mixtures (HIF1α, Biolegend; EZH2 ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubation with Human TruStain FxC (10 minutes at 4°C; Biolegend, #422302) to block Fc receptor-mediated binding ...
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bioRxiv - Immunology 2020Quote: ... and cultured for 4 days +/- αIL10 antibody +/- isotype control (5μg/ml, both Biolegend). Assessment of the direct effect of IL-10 on human CD4 T cells was performed by plate bound αCD3/28 (1μg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... Remaining volume was incubated at 4°C with anti-HA antibody (Biolegend, #901513) at 1:150 dilution for 4hrs on a gentle spinner ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed for 10 min at 4°C using IC fix (Biolegend) and cells were then incubated in for 20 min at RT in Permeabilization buffer (biolegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... we added 4 μg/ml PE-labeled anti-CD63 antibody (BioLegend, clone H5C6). We imaged the nanowell using Zeiss Axio Observer Z1 microscope equipped with 20x/0.8 NA objectives and a Hamamatsu Orca Flash v2 camera.
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bioRxiv - Immunology 2023Quote: ... Cells were then washed and stained with CTLA-4-PE-Dazzle 594 (Biolegend), Ki67-PerCP-eFluor 710 (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... or Th2-polarizing condition (10 ng/mL recombinant mouse IL-4 (#574304, BioLegend), and 10 μg/mL anti-mouse IL-12/23p40 antibody (clone C17.8 ...
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bioRxiv - Developmental Biology 2023Quote: ... additional staining with anti-CD34 (4 µL CD34 APC/Cy7, clone: 581, Biolegend) and anti-CD14 antibodies (3 µL CD14 PE-CF594 ...
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bioRxiv - Immunology 2023Quote: ... 4 µL of cocktail containing PMA/ionomycin + Brefeldin A (Biolegend, San Diego, CA) was added ...
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bioRxiv - Neuroscience 2024Quote: ... coated with 4 µg 77G7 anti-tau antibody (MTBR epitope 316-355, BioLegend) per sample ...
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bioRxiv - Immunology 2024Quote: CD9 antibody (Clone: HI9a, Biolegend, Cat-no. 312102; 4 μl in 1ml PBS) was used to coat a 48-well plate ...
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bioRxiv - Biochemistry 2024Quote: ... followed by overnight incubation at 4°C with 6E10 (BioLegend, Cat. No. 803002) or 4G8 (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... monocytes were stimulated with IL-4 (50 ng/mL, BioLegend, catalog no. 574002). 50 µL of medium containing M-CSF and IL4 was replenished every other day for 7 days ...
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bioRxiv - Systems Biology 2021Quote: Cells were stained overnight at 4°C in blocking solution with the following antibodies: AlexaFluor® 488 anti-Vimentin (1:1000, Biolegend, #677809), AlexaFluor® E-cadherin (1:200 ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated overnight at 4°C with purified monoclonal mouse anti-human ASC antibodies (TMS-1) (clone HASC-71) (Biolegend, California, USA) at a final concentration of 10 µg/ml diluted in 10% NGS + 0.5% Triton X-100 in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... samples P008-016 and P048-119 (Suppl. Fig. 4D) were labelled with TotalSeq B Hashtag antibodies 1-4 (Biolegend, clone LNH-94) during the staining procedure described above and pooled in groups of four.
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bioRxiv - Immunology 2021Quote: ... Slides were incubated ON at 4°C with mouse anti-human CD66b antibody (BioLegend, 305102, 1:100 for both IHC and IF), anti-human CD8 (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Cells were harvested and stained for 30min at 4°C with the following antibodies: anti-CD25 BV785 (Biolegend, Cat: 356139, 1:50), anti-CD127 PE (Biolegend ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated 72h at 4°C on a rotating wheel with the secondary antibody (1:500, Alexa-Fluor-555, 405324 P4U/BioLegend UK Ltd) diluted in 100% CAS-Block ...
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bioRxiv - Immunology 2024Quote: ... and then surface labelled for 30 min at 4°C with TotalSeq-C0182 anti-mouse CD3 (clone 17A2, dilution 1:50, Biolegend, cat # 100263) and TotalSeq-C0002 anti-mouse CD8α (clone 53-6.7 ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were incubated with the following antibodies for 30 min at 4°C: CD45.1-PerCP-Cy5.5 (BioLegend, Catalog No. 110728, 1/500), CD45.2-BV650 (BioLegend ...
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bioRxiv - Cell Biology 2023Quote: ... the pellet was resuspended in PBS and incubated for 30 min at 4 °C with rat FITC-conjugated mAb against CD11b (1:200, BioLegend, CA, USA). After incubation ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted down at 1,500 rpm at 4°C for 5 min and cell pellets were resuspended in 100 uL FACS buffer (PBS + 4% FBS +0.5 mM EDTA) containing Fc block (BioLegend, 101302, 1:200). After 15 min incubation at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were incubated with antibody cocktails in blocking buffer overnight at 4°C in a moisture chamber: Amyloid-β (6E10) (1:100; Biolegend, 803001), anti-CHMP2B (1:100 ...
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bioRxiv - Immunology 2023Quote: ... for 10 min at RT then stained for the following cell surface markers for 30 min at 4°C: CD3 (T cell marker, mouse anti-CD3-BV510, BioLegend, 1:50), CD14 (monocyte marker ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in PBS and stained at 4°C with 100 µL 1:500 Zombie Aqua viability dye (BioLegend, Cat# 423102) and 1:200 mouse FC block (TruStain FcX ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 30 minutes at 4 °C using antigen probe (1.5 μL/well) and the following conjugated antibodies: APC anti-human CD3 (300412, BioLegend; 1 μL/well), PerCP/Cyanine 5.5 anti-human CD19 (302230 ...
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bioRxiv - Immunology 2024Quote: ... for 40 minutes at 4 °C followed by a secondary staining with anti-mouse IgG (1:200, Poly4053-PE- Cy7, Biolegend, Cat # 405315) or anti-mouse IgM (1:200 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were fixed with 4% PFA in PBS and exposed to 1:1000 anti-HA mouse monoclonal antibody (BioLegend Cat#901514, RRID:AB_2565336) or 1:1000 anti-FLAG mouse monoclonal antibody (M2 ...
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bioRxiv - Cancer Biology 2020Quote: ... coated with intercellular adhesion molecule (ICAM-1, ICAM-2) and vascular cell adhesion molecule (VCAM-1) antibodies conjugated to Alexa Fluor 680 (BioLegend, San Diego, CA). Sorted cells were cultured in Endothelial Growth Media (EGM-2 ...
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bioRxiv - Cell Biology 2023Quote: ... Between 50,000 to 100,000 cells were resuspended in 10 uL of PBS supplemented with 2% FBS and 2 mM EDTA (hereafter named “FACS buffer”) together with 5 uL of TruStain FcX (BioLegend, San Diego, CA, USA). Cells were incubated with the FcX block for 10 minutes at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... CD18 (M18/2) and CD62L (MEL-14; all from Biolegend). In some experiments ...
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bioRxiv - Bioengineering 2021Quote: ... AlexaFluor488 anti-Bcl-2 and BV711 anti-CD11b antibodies (Biolegend) were used for staining of in vivo NP-treated alveolar and peritoneal macrophages ...
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bioRxiv - Immunology 2022Quote: ... 2 μL PE/Cyanine7 anti-human IgD antibody(BioLegend, 348210), 0.0032 μg biotinylated SARS-CoV-2 BA.1 protein (His & AVI Tag ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-TNF-α BV785 and anti-IL-2 FITC (Biolegend) in PermWash buffer according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... 2 μL PE/Cyanine7 anti-human IgM antibody (BioLegend, 314532), 2 μL PE/Cyanine7 anti-human IgD antibody(BioLegend ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 ΔRBM RBD-APC (streptavidin-APC from BioLegend). Conjugations were performed as previously described (65) ...
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bioRxiv - Bioengineering 2020Quote: ... ELISA plates were coated with anti-IL-2 Ab (BioLegend) at a concentration of 0.25ug/ml overnight at 4 °C or 1 h at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... CD90-2 (alexa fluor 700 AM; clone 30-H12, BioLegend), B220 (CD45R ...
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bioRxiv - Immunology 2022Quote: ... IL-2 was also neutralized (JES6-1A12, BioLegend, 5ug/mL). Cells were cultured on stimulation for 72-96h before analysis.
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bioRxiv - Cancer Biology 2021Quote: ... suspended in culture medium containing IL-2 (10ng/ml; BioLegend). Cells were directly stained with CFSE (Cayman Chemical ...
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bioRxiv - Immunology 2020Quote: ... anti-IL-2-PE/Cy7 (clone MQ1-17H12, BioLegend, USA).
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bioRxiv - Immunology 2021Quote: ... and biotin anti-mouse IL-2 (clone JES6-5H4, BioLegend) antibody was used as the secondary antibody ...
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bioRxiv - Immunology 2021Quote: ... LEAF™ purified anti-CD18 (clone M18/2, 101409, BioLegend), LEAF™ Purified Rat IgG2a ...
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Epigenetic alterations underlie airway macrophage differentiation and phenotype during lung fibrosisbioRxiv - Immunology 2020Quote: ... and human CD206 APC-Cy7 (clone 15-2, BioLegend, USA). CD206+ cells were enriched in MACS LS magnetic separation column and MidiMACS™ magnet ...