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Citations for BioLegend :
851 - 900 of 7971 citations for 5 Pyrimidinecarbonitrile 1 2R 2 3 dihydroxypropyl 1 2 3 4 tetrahydro 3 methyl 2 4 dioxo 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... then incubated for 30 minutes at 4°C with pre-chilled 4°C PBS (200 uL) containing 4 uL FITC-conjugated anti-CD63 (clone H5C6, Biolegend) and 4 uL APC conjugated anti-CD9 (clone H19a ...
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bioRxiv - Genomics 2020Quote: ... cells were resuspended in PBS complemented with 2% fetal bovine serum and stained with FITC anti-human CD45 antibody (BioLegend #304006CD45, 1:200 dilution) and PE anti-human EPCAM antibody (Miltenyi Biotech #130-113-264 ...
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bioRxiv - Immunology 2023Quote: ... pDCs were gated for flow cytometry with 1:20 dilution commercial mouse anti-human BDCA-2-Alexa488 (stock 200 ug/mL, BioLegend, San Diego, CA, USA) and 1:20 dilution mouse anti-human CD123 (IL-3)-PE (stock 200 ug/mL ...
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bioRxiv - Immunology 2023Quote: ... TCR-transduced CD8+ T cells were rapidly expanded for 5 days using irradiated feeder PBMCs and medium containing 60U/ml IL-2 (Proleukin, Novartis) and Ultra-LEAF™ Purified anti-human CD3 Antibody (clone OKT3, BioLegend 317326, 1:2000). After 5 days ...
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bioRxiv - Immunology 2021Quote: ... CD11a (M17/4, Biolegend), CD27 (LG-7F9) ...
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bioRxiv - Immunology 2021Quote: ... and IL-4 (BioLegend) were prepared in PBS at concentrations of 1000 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... CD11a (M17/4; Biolegend), and CD69 (H1.2F3 ...
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bioRxiv - Immunology 2022Quote: ... IL-4 (BioLegend, 574004), IL-13 (BioLegend ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were stained for 30 min at +4°C with APC or PE-conjugated anti-CD133 antibodies (diluted at 1/200 or 1/400 respectively; Biolegend, San Diego, USA) or corresponding control Immunoglobulin G1 (IgG1 ...
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bioRxiv - Molecular Biology 2024Quote: ... according to manufacturer’s recommendations and stained for 1 hour at 4°C with the following antibodies: NGFR (345110; 1:600, BioLegend, San Diego, CA, USA), α-tubulin (NB100-69AF405 ...
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bioRxiv - Immunology 2023Quote: ... and stained for 5 min at 4°C with biotinylated anti-CD3 antibody (17A2; BioLegend) to exclude CD3-positive T cells in the cell suspension using anti-biotin microbeads (Miltenyi Biotec ...
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bioRxiv - Microbiology 2020Quote: ... The blot was then washed 3 times in PBS-T and probed with goat anti-rat HRP (Biolegend) and goat anti-mouse HRP (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... 96-well ELISA plates were coated with 3 μg/ml GPI-RBD-GM-CSF in coating buffer (BioLegend) overnight at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed 3 times in FACS buffer and incubated in Cyto-Fast Fix/Perm Buffer (BioLegend, 426803) for 20 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... frozen PBMC were thawed and stained with 3 μl of anti-human CD5-APCeF780 antibody (clone L17F12, Biolegend), 2 μl of anti-human CD19-PECy7 antibody (clone HIB19 ...
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bioRxiv - Immunology 2021Quote: ... The first surface marker panel included: BV421 and PerCP/Cy5.5 anti-TIM-3 (clones B8.2C12 and RMT3-23, BioLegend), BV510 anti-PD-1 (EH12.1) ...
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bioRxiv - Physiology 2022Quote: ... 3 106 cells were then incubated with Fc receptor blocking solution (Human Trustain FcX, Biolegend, San Diego, USA) for 5 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 3× before being incubated with APC-conjugated rat anti-CD83 antibody (1.25 μL/10×106 cells; Biolegend, 121510) for 30 minutes on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells were again washed 3 times before adding 50 µL per well of HRP Streptavidin (#405210, BioLegend, USA) diluted 1:2000 in 1% SureBlock solution and incubated 1 h at RT ...
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bioRxiv - Immunology 2023Quote: ... Cells were plated in a 96-well round-bottom plate coated with 3 μg/ml anti-CD3 (Biolegend), uncoated well or with PMA (1 ng/ml ...
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bioRxiv - Synthetic Biology 2024Quote: ... and stained for the intracellular accumulation of IFNγ and TNFα (Supp. Table 3) in 1x Permeabilization Buffer (Biolegend). Samples were analyzed using a Fortessa LSR flow cytometer (BD ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated with secondary antibody diluted in 3% BSA PBS (Alexa Fluor 488 Goat anti-mouse IgG, BioLegend, 405319) in the dark for 1 hour at room temperature and washed 3x with PBST ...
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bioRxiv - Immunology 2024Quote: ... and blocked using 3% FCS or mouse serum in Facs buffer and/or Tru block monocyte blocker (Biolegend) as required ...
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bioRxiv - Immunology 2022Quote: ... or DC in vitro culture (5×105) were washed with PBS at 400 g for 5 min at 4°C and blocked with FcX (BioLegend) for 15 min ...
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bioRxiv - Bioengineering 2021Quote: ... which were incubated overnight at 4 °C with primary antibodies diluted 1:5,000 in blocking buffer (BioLegend Way ...
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bioRxiv - Cell Biology 2023Quote: ... they were incubated at 4°C overnight in primary antibodies against anti-HA (1:100; BioLegend; 901533), CA3 (1:150 ...
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bioRxiv - Neuroscience 2024Quote: ... wells were incubated overnight at 4°C with mouse anti-β-III-tubulin (1:500, Biolegend, #MMS435P). After washing with PBST ...
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bioRxiv - Immunology 2021Quote: ... 2 cell populations were sorted and barcoded separately with TotalSeq antibodies (BioLegend) before mixing and cell captures (Supplemental Data 1) ...
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bioRxiv - Immunology 2020Quote: ... antibodies with the presence of 20 ng/μl IL-2 (Biolegend #589106) for 3 days.
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bioRxiv - Biochemistry 2020Quote: ... and rat anti-mouse CD4 F(ab’)2 fragment (clone GK1.5, BioLegend, fragmented according to published procedures45 ...
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bioRxiv - Cell Biology 2021Quote: ... was used at 2 μg/ml and soluble anti-mouse CD28 (Biolegend) was used at 8 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 ΔRBM RBD-PE/Cy5.5 (streptavidin-PE/Cy5.5 from BioLegend). The panel for the cohort that received the SARS-CoV-2 spike prime followed by the RBD homotrimer cocktail boost also included SARS-CoV RBD-APC (streptavidin-APC from BioLegend ...
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bioRxiv - Microbiology 2019Quote: ... and CD8 (2 μg/ml APC/Cy7 a-mouse CD8a antibody; Biolegend) in PBS for 20 min before fixation in 2% paraformaldehyde (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2019Quote: ... and IFN-γ (2 μg/ml APC a-mouse IFN-γ, Biolegend) prior to analysis on a flow cytometer (LSR Fortessa X-20 ...
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bioRxiv - Immunology 2021Quote: ... and anti-mouse-IL-2-PE-Cy7 (Biolegend Cat# 503832, RRID: AB_2561750). Stained cells were acquired by FACSAriaIII Flow Cytometer (BD Biosciences ...
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bioRxiv - Pathology 2021Quote: ... pan aSyn (N-terminal directed, SYN303 [aa 2-12 Cat# 82401, Biolegend] ...
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bioRxiv - Cell Biology 2021Quote: ... and 2 μg/ml soluble anti-mouse CD28 antibody (102116, BioLegend, USA) in RPMI 1640 medium (21870-076 ...
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bioRxiv - Immunology 2021Quote: ... PE-anti-human-Nectin-2 (TX31) (all from BioLegend, San Diego, CA). The following antibodies were used for in vivo blocking experiments ...
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bioRxiv - Immunology 2020Quote: ... KLRG1 APC-Cy7 (clone 2FI/KLRG1, BioLegend, cat# 138426, 2:100 dilution); CD279 (PD-1 ...
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bioRxiv - Neuroscience 2022Quote: ... Fisher) along with 2 µl of live/dead cell stain (DRAQ7, BioLegend) per ml and then FACS sorted.
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bioRxiv - Immunology 2022Quote: ... Rat anti-CD138 antibody (rat anti-CD138, clone 281-2, Biolegend, 142502) was applied to each section and specific bounds were visualized using a Alexa Fluor488-conjugated anti-rat antibody (JacksonImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... or 2 ug/ml PE-conjugated anti-mouse TLR2 antibodies (BioLegend, 148603) for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Culture supernatants were collected and analyzed for IL-2 by ELISA (BioLegend).
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bioRxiv - Immunology 2023Quote: ... Cells were washed with 2× Cyto-FastTM Perm Wash solution (CFPWS; BioLegend) and incubated with PGP9.5 for 20 min at RT ...
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bioRxiv - Immunology 2024Quote: ... and with 100 U/ml IL-2 (BioLegend, San Diego, CA, USA) (referred to as “Bead activated T cells” or “nonspecific activation” ...
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bioRxiv - Immunology 2024Quote: ... the gels were incubated with 2 μg/ml Calcein-AM (BioLegend, 425201) and 1 μg/ml Propidium Iodide (MP Biomedicals ...
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bioRxiv - Immunology 2024Quote: ... Media was additionally supplemented with 10 ng/mL murine IL-2 (BioLegend) prior to resuspension and subsequent passaging ...
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bioRxiv - Cancer Biology 2022Quote: ... 50 μL of interleukin-4 (IL-4, 50 ng/mL, BioLegend, cat. 574002) and M-CSF (100 ng/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed with 2% neutral-buffered formalin diluted in FACS buffer following addition of Zombie Yellow fixable viability dye (BioLegend #423103, 1:200 in PBS).
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bioRxiv - Molecular Biology 2023Quote: ... The cells were resuspended in 10 μl of FACS Buffer and incubated with 1 μl of allophycocyanin (APC)-conjugated anti-CD46 antibody (BioLegend, clone TRA-2–10, 352405) for 30 min at 4°C with rotation ...