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Citations for BioLegend :
1101 - 1150 of 7971 citations for 5 Pyrimidinecarbonitrile 1 2R 2 3 dihydroxypropyl 1 2 3 4 tetrahydro 3 methyl 2 4 dioxo 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Naive P14 Thy1.1+ Il2ra−/− cells were isolated from 4-5 week old mice by depleting CD44hi cells (Biolegend biotin anti-mouse/human CD44 ...
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bioRxiv - Cancer Biology 2024Quote: Pre-coat a 12-well plate overnight at 4 °C using CD3 antibody (5 μg/ml, 317326, Biolegend), and before inoculation ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight at 4°C with 8µg of biotinylated anti-CD4 antibody (clone RM4-4, BioLegend). Prior to immunoprecipitation (IP) ...
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bioRxiv - Immunology 2022Quote: ... Cell surface expression of CTLA-4 was measured by adding anti-CTLA-4-PE-Dazzle594 mAb (BioLegend) in culture during restimulation for 24 h ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4°C with 8μg of biotinylated anti-CD4 antibody (clone RM4-4, BioLegend). Prior to immunoprecipitation (IP) ...
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bioRxiv - Immunology 2019Quote: ... and stained with antigen presenting cells conjugated anti-ERK1/2 pThr202/Thy204 (Biolegend, clone 6B8B69) overnight at 4°C in FOXP3 perm buffer (Biolegend) ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... resuspended in 1x DAPI solution (in PBS plus 0.5% BSA and 2 mM EDTA; BioLegend) at 5×106 cells/ml ...
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bioRxiv - Immunology 2021Quote: ... Cells were subsequently blocked with 2 μL of TruStain FcX Fc receptor blocking antibody (BioLegend) for 10 minutes on ice ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then stained with Brilliant Violent 421-conjugated ms α-EGFR (Biolegend; Table 2). Samples were gated for intact GFP+ cells and geometric mean of fluorescence intensity (geoMFI ...
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bioRxiv - Immunology 2021Quote: ... Lung cells were plated at 2 million cells/well and incubated with Zombie UV (Biolegend) for dead cell discrimination ...
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bioRxiv - Microbiology 2023Quote: ... we administered 2 μg of APC/Fire750-labeled anti-CD45 mAb (Biolegend, clone 30-F11) in mice anesthetized with an overdose of ketamine and xylazine ...
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bioRxiv - Immunology 2023Quote: ... The 96-well ELISA plates were pre-coated with purified anti-mouse IL-2 (BioLegend) using coating buffer (0.1 M Na2HPO ...
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bioRxiv - Biophysics 2023Quote: ... and 6.25 μg/mL activation antibodies against Integrin β2 (clone m24 and LFA1/2, Biolegend) were added in cell culture medium at the start of the culture.
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bioRxiv - Cancer Biology 2023Quote: ... and Jagged1/2 blocking antibodies and IgG isotype control (Biolegend, cat#s 130902, 131001, 400902) were used at 20 µM/ml.
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bioRxiv - Microbiology 2023Quote: ... and 0.1% mercaptoethanol) supplemented with 2 μL Cell Activation Cocktail with Brefeldin A (Biolegend, #423304) and incubated for 4 h at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... The cells were then incubated overnight with rat anti-CD14 IgG (clone Sa14-2; BioLegend) and goat anti-GST IgG (Rockland ...
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bioRxiv - Genomics 2023Quote: ... cells were incubated in the antibody mix (2 μg/ml rat anti-CD326 (BioLegend 312502), 1 μg/ml rabbit anti-RAD51-AF647 (Bioacademia 70-012) ...
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bioRxiv - Immunology 2024Quote: ... The cells were then stained with a cocktail of cell surface antibodies (Biolegend, Table 2) in 0.5%BSA/PBS and analyzed on a LSR II flow cytometer (BD Biosciences) ...
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bioRxiv - Systems Biology 2024Quote: ... Staining was performed with FITC-coupled mouse monoclonal anti-ERK1/2 phospho (Thr202/Tyr204, Biolegend) antibody (diluted 1:200 in staining buffer ...
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bioRxiv - Bioengineering 2024Quote: ... Each tube of cells then received 2 µL of Fc block (BioLegend, San Diego, CA), 10 µL of Brilliant Stain Buffer (Becton Dickinson) ...
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bioRxiv - Immunology 2022Quote: ... Cytokine staining of splenocytes after attLm-OVA infection was carried out after re-stimulation of CD8 T cell enriched splenocytes with 50 nM SIINFEKL peptide for 3 hours with Brefeldin A (Biolegend, cat#:420601).
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected 3 days after coculture initiation and used to quantify T cell activation levels with ELISA (BioLegend, Cat no. 430801) following the manufacturer’s protocols.
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bioRxiv - Immunology 2024Quote: ... Primary lymphocytes isolated from skin draining lymph nodes from adult C57BL/6 mice were cultured on plates precoated with anti-mouse α-CD3 (3 μg/ml, Biolegend, #100340) and anti-mouse α-CD28 (3 μg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Slides were incubated with the following primary antibodies diluted in blocking buffer overnight at 4°C – chicken anti-keratin 14 (1:800, Biolegend 906001), rabbit anti-SCD1 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated with the following primary antibodies overnight at 4 : mouse anti-C3 (1:200 dilution; Biolegend cat. no. 846302) + rabbit anti-GFAP (1:500 dilutio L gilent Dako cat ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocking of Fc receptors was performed by incubation at 4°C for 20 minutes with an unlabelled anti-CD16/32 (1 μg/million cells; BioLegend – 101320). The cells were then incubated for one hour at 4°C in the dark with a mix of rat anti-mouse CD41 (labelled with Phycoerythrin ...
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bioRxiv - Cancer Biology 2019Quote: ... and stained with the following antibodies in isolation or in combination for 30-60 minutes in the dark at 4°C: ICAM-1 PE (BioLegend, #353106), CCR6 PE/Dazzle (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained with the following primary mouse antibodies (1:100 dilution) for 30 minutes at 4°C: CD45-BV750 (clone 30-F11, Biolegend, 103157), CD31-PE-Cy5.5 (clone MEC 13.3 ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were incubated overnight at 4 °C with primary antibody (Biotin anti-β-Amyloid, 1-16 Antibody, clone 6E10 from BioLegend) dissolved in a carrier solution made with 1% HS ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used in this study: Alexa Fluor 488 anti-human SSEA-4 (#330412, clone MC-813-70, Mouse IgG3 κ, dilution 1:100, Biolegend); Alexa Fluor 647 anti-human SSEA-4 Antibody (#330408 ...
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bioRxiv - Immunology 2022Quote: ... Immunostaining was performed by incubating the sections overnight at 4°C with the SMI-32 primary antibody (Biolegend: Suppl. Table 1) in incubation buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... for 10 min at 4°C followed by an incubation with CD45-BV421 (Biolegend 103133, clone 30-F11, 1:200 dilution) and Ter119-BV421 (Biolegend 116233 ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue sections were stained with monoclonal mouse anti-βIII-tubulin primary antibody (Biolegend, Cat. 801202; 1:100 overnight at 4 °C) and goat anti-mouse AF647 (abcam ...
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bioRxiv - Immunology 2023Quote: ... surface markers were stained with following antibodies at 1:200 dilution at 4°C for 30 min: CD11b- Pacific Blue (BioLegend, 101224), CD45-BV650 (BioLegend ...
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bioRxiv - Cell Biology 2022Quote: ... The digested tissue was resuspended in 100 μL of 4% FBS in PBS containing an FITC antibody to CD11b (1:100; BioLegend, 101206) and PE antibody to CD45(1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... cell suspensions were stained for 30 min at 4°C in the dark with CD45-BV605 (1:50, BioLegend #304042, RRID:AB_2562106), ITGA6-PeCy7 (1:100 ...
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bioRxiv - Immunology 2022Quote: ... 4°C) and preincubated for 15min with anti-CD16/CD32 Fc-block antibody (10μg/ml; BioLegend, San Diego, CA, 1:50) in FACS buffer followed by a 30min incubation with fluorescence dye-conjugated ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells transfected with pMAX-GFP and either pcDNA3.1-Rai or empty vector were added in a 10:1 ratio and incubated for 4 h in presence of APC anti-LAMP1 antibody (clone H4A3, Biolegend #328620). Monensin (2mM ...
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bioRxiv - Neuroscience 2023Quote: ... After incubation, cells were centrifuged (350rcf, 7min, 4°C) and resuspended in permeabilization buffer containing Streptavidin (PerCP, Biolegend #405213, 1:600) and antibodies directed against intracellular targets Foxp3 (APC ...
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bioRxiv - Bioengineering 2024Quote: ... were coated with 100 µL of 4 µg/mL polyclonal anti-lysostaphin antibody (AICBIOTECH-PAb102) in 1×ELISA coating buffer (BIOLEGEND-421701). Wells were washed with 0.05% Tween 20 (SIGMA-P9416-50ML ...
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bioRxiv - Immunology 2022Quote: ... culture media was removed and replaced with media containing either 50 ng/mL interleukin 4 (IL-4) (BioLegend) for 48 hr to generate M2 macrophages or 50 ng/mL interferon-gamma (IFN-γ ...
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bioRxiv - Cancer Biology 2023Quote: ... and supplemented with sCTLA-4 conditioned media or murine CTLA-4-Fc fusion protein (10 µg/mL, BioLegend). After 4 days ...
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bioRxiv - Cancer Biology 2022Quote: ... or TFH mimicking signals (including 0.02 μg/mL recombinant human IL-4, Peprotech; 0.05 μg/ml recombinant human IL-21, Peprotech, and 5 μg/mL anti-human CD40, Biolegend). Complete RPMI medium contained RPMI 1640 (Gibco ...
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bioRxiv - Immunology 2020Quote: ... Th1 cells were differentiated for 4 days in the presence of plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-12 (25 ng/ml ...
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bioRxiv - Microbiology 2021Quote: ... Primary CD4+ T cells were activated for 4-5 days on T25 flasks coated with 5μg anti-CD3 antibody (clone OKT3, Biolegend) in the presence of 2μg/ml soluble anti-CD28 antibody (clone CD28.2 ...
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bioRxiv - Immunology 2020Quote: The following antibodies and reagents were used for flow cytometry: CD4 clone RM-4-5 (BD Biosciences, BioLegend, Tonbo), CD8α clone53-6.7 (BD ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse anti-TNFRSF12A (clone ITEM-4, Biolegend) was utilized for (Fig ...
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bioRxiv - Developmental Biology 2020Quote: ... 4 µg of anti-RNA polymerase II CTD repeat YSPTSPS antibody [8WG16] from Abcam (ab817) and 4 µg from BioLegend (664912) were used for each sample ...
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bioRxiv - Cell Biology 2021Quote: ... and 10 ng/ml IL-4 (Biolegend) for 9 days ...
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bioRxiv - Microbiology 2020Quote: ... anti-CD4 (2µg/ml, RM4-4; Biolegend), anti-CD3 (2µg/ml ...