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Citations for BioLegend :
8601 - 8650 of 8754 citations for Recombinant Human Interleukin 1 Beta since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... and anti-mouse CD44(CD44-APC - cat. 137607, clone 29A1.4, LOT B152186, dilution 1:200) were purchased from BioLegend (BioLegend, London, UK). Antibody dilutions in cell staining buffer containing 1 % TruStain fcX™ (anti-mouse CD16/32 ...
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bioRxiv - Cell Biology 2021Quote: ... and then resuspended in 10% FCS in PBS (100μl/well) containing TruStain FcX™ (anti-mouse CD16/32) antibody (Biolegend; 1:200) and incubated on ice for 20mins ...
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bioRxiv - Immunology 2019Quote: ... TCRβ (H57-597, BV605), NK-1.1 (PK136, BV650), and PD-1 (RPM1-30, PE/Dazzle 594) were purchased from BioLegend (San Diego, CA). Antibodies against CD4 (GK1.5 ...
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bioRxiv - Immunology 2020Quote: ... the cells were washed with 1 ml of PBS and finally resuspended in 100 µl of FACS buffer (1% BSA in PBS with Ca2+/Mg2+) with anti-HMGB1 antibody (10µg/ml, BioLegend, catalogue number-651402). The cells were incubated at 4 °C for 30 minutes on ice and were washed with 1 ml PBS (containing Ca2+/Mg2+) ...
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bioRxiv - Plant Biology 2021Quote: ... Biotin labeled DNA was detected with 1:5000 solution of an anti-biotin HPR-conjugated antibody (BioLegend, San Diego, California, United States) in TBST with 5% BSA ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: In order to separate cells of different origin we further stained the cell suspension with antibodies against CD45 (Biolegend #103107; 1:200), FAP (Abcam #ab28244 ...
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bioRxiv - Neuroscience 2020Quote: ... Cell suspensions were incubated for 20 minutes on ice with anti-CD16/CD32 to block Fc receptors (1:50; BioLegend Cat #: 553141) and with a viability dye ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μL of CytoC stain buffer (2% Tween20, 10% BSA (w/v) in PBS) + 1:400 Alexa Fluor 647 anti-cytochrome c antibody (Biolegend, cat#612310) + 1:100 DAPI (1 mg/mL ...
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bioRxiv - Bioengineering 2020Quote: ... cells were washed 3x with PBS and incubated with a cocktail of primary antibodies to intracellular compartment markers - clathrin (1:500, Biolegend MMS-423P), caveolin (1:200 ...
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bioRxiv - Immunology 2021Quote: ... a set of plated moDCS were treated with an anti-HLA-A/B/C antibody (40 μg mL−1, Clone W6/32, Biolegend Cat 311428) or anti-class II (10 μg mL−1 ...
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bioRxiv - Immunology 2021Quote: ... infarct macrophages as CD45+/CD11b+/Ly6G−/F4/80+ cells and infarct neutrophils as CD45+/CD11b+/F4/80−/Ly6G+ cells.7–9 The following antibodies were used: anti-CD45 (clone 104, 1:100, BioLegend/BD Biosciences); anti-F4/80 (clone BM8 ...
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bioRxiv - Cell Biology 2020Quote: ... a 6-colour flow cytometry panel was performed on a Bio-Rad ZE5 Cell Analyzer using antibodies for CD56 (Clone HCD56, Brilliant Violet 605, BioLegend, 1:200), CD3 (Clone SK7 ...
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bioRxiv - Neuroscience 2021Quote: ... that recognizes residues 17–24 of Aβ and the same sequence in APP full length (BioLegend San Diego, California, USA; 1:1000); mouse C-term APP Y188 (Abcam ...
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bioRxiv - Bioengineering 2022Quote: ... The samples were treated with 3% bovine albumin in PBS containing 0.3% Triton X-100 and then stained with CD31 antibody conjugated with Alexa 647 (1:100 dilution, Biolegend, San Diego, CA) and EMCN antibody conjugated with PE (1:50 dilution ...
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bioRxiv - Immunology 2022Quote: ... lymph node FNA samples collected 2 or 8 weeks post-boost were stained for 30 min on ice with purified CD16 (3G8, BioLegend, 1:100), CD32 (FUN-2 ...
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bioRxiv - Bioengineering 2024Quote: ... were first stained with Zombie Aqua™ fixable viability kit (1:500 dilution in PBS) and Fc-blocked with TruStain FcXTM (anti-mouse CD16/32) antibody (BioLegend, CA, U.S.A.) (1:20 dilution in PBS (1% BSA) ...
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bioRxiv - Cancer Biology 2024Quote: ... Pelleted nuclei were suspended in Nuclear Stain Buffer (Sucrose Buffer containing 1:4,000 Alexa Fluor® 647 anti-Nuclear Pore Complex Proteins Antibody (BioLegend, Cat# 682204) plus 4µM ethidium homodimer (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... GAPDH was used as an internal control (1:1000, Direct-Blot™ HRP anti-GAPDH antibody, 607903; Biolegend, San Diego, CA, USA). Signals were visualized using Luminata Forte (Merck Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... the pellets were resuspended and incubated for 15 min on ice in 0.5 ml MEM containing CD24-APC antibody (1:500, 101814, Biolegend, San Diego, CA). After centrifugation for 5 min at 200 × g at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... and then surface labelled for 30 min at 4°C with TotalSeq-C0182 anti-mouse CD3 (clone 17A2, dilution 1:50, Biolegend, cat # 100263) and TotalSeq-C0002 anti-mouse CD8α (clone 53-6.7 ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were incubated with the following antibodies for 30 min at 4°C: CD45.1-PerCP-Cy5.5 (BioLegend, Catalog No. 110728, 1/500), CD45.2-BV650 (BioLegend ...
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bioRxiv - Developmental Biology 2024Quote: ... The APC depleted fraction was collected and used for staining with the following antibodies: Ter119-FITC (BioLegend, Catalog No. 116205, 1/800), Cd45R-FITC (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... The CD11c-APC-depleted fraction was further incubated with anti-biotin beads (Milteniy Biotec, cat# 130-090-485) and streptavidin-PE/Cy7 (BioLegend, 1:500), followed by another MACS enrichment using MS columns ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated 72h at 4°C on a rotating wheel with the secondary antibody (1:500, Alexa-Fluor-555, 405324 P4U/BioLegend UK Ltd) diluted in 100% CAS-Block ...
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bioRxiv - Immunology 2023Quote: ... IL-1b, IL-6, IL-10, IL-17A, IL-12p70, GM-CSF, IL-23, IFN-b, MCP-1, IL-27; BioLegend Cat# 740446), Mouse TH Panel v4 (TNF-α ...
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bioRxiv - Cell Biology 2023Quote: ... the pellet was resuspended in PBS and incubated for 30 min at 4 °C with rat FITC-conjugated mAb against CD11b (1:200, BioLegend, CA, USA). After incubation ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies used and the dilutions were as follows: for neurons: anti-Tubulin-βIII Rabbit mAb (1:200, BioLegend, 802001, clone TUJ1); anti-Synapsin-1 Mouse mAb (1:200 ...
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bioRxiv - Bioengineering 2023Quote: ... T-cells that have been incubating with conditioned media were carefully transferred to the CD3-coated plates and further stimulated with 1 µg/mL anti-CD28 (BioLegend, clone: CD28.2) for another 48 hours ...
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bioRxiv - Microbiology 2023Quote: ... Tissue was rinsed 3 times in 1X PBS and then incubated with 1:5000 dilution rabbit polyclonal anti-Keratin-14 antibody (Biolegend, Clone Poly19053) for 1 hour and then detected with 1:10000 dilution goat anti-Rabbit IgG Secondary Antibody conjugated with Alexa Fluor™ 488 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted down at 1,500 rpm at 4°C for 5 min and cell pellets were resuspended in 100 uL FACS buffer (PBS + 4% FBS +0.5 mM EDTA) containing Fc block (BioLegend, 101302, 1:200). After 15 min incubation at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... the CNP labeled cells were spin washed and incubated with 10μgꞏml−1 of AF488 conjugated anti-CD3 antibodies (Biolegend, Cat # 300415, clone UCHT1) in 2% BSA/PBS on ice for 30min and then spin washed ...
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bioRxiv - Cancer Biology 2023Quote: ... we triple-washed samples with 0.1% PBS-Tw and labeled them with streptavidin-BV510 (Biolegend, 405234; 1% BSA in 0.1% PBS-Tw). After a 30-min incubation ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor single-cell suspensions were stained with Alexa Fluor 700 anti-CD326 (Ep-CAM) antibody (Biolegend, 118239, clone: G8.8, 1:100 dilution), PE/Cyanine 7 anti-mouse CD31antibody (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... then were resuspended in FACS (PBS + 3% FBS + 1mM EDTA + 10mM HEPES) supplemented with TruStain FcX (1:50; BioLegend; San Diego, CA) and TruStain Monocyte Blocker (1:20 ...
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bioRxiv - Immunology 2023Quote: ... Cells were suspended in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302, 1:400) and stained with antibodies at a 1:400 dilution against the following mouse markers ...
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bioRxiv - Immunology 2023Quote: Serum BAFF quantitation was performed on plasma samples from 12-14 week-old Sh2b3E372K mice using the LEGENDplex Mouse B cell Panel – S/P (1-plex) w/VbP kit (BioLegend; cat # 740983) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were incubated with antibody cocktails in blocking buffer overnight at 4°C in a moisture chamber: Amyloid-β (6E10) (1:100; Biolegend, 803001), anti-CHMP2B (1:100 ...
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bioRxiv - Immunology 2022Quote: ... CD3ε clone 145-2C11; Ly-6G/Ly-6C clone RB6-8C5; CD11b clone M1/70; CD45R/B220 clone RA3-6B2; TER-119, Biolegend, 1/200). The c-kit− fraction was stained with antibodies against CD11b (PercPCy5.5 ...
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bioRxiv - Immunology 2023Quote: ... cells were washed twice with PBS and stained using the Zombie NIR Fixable Viability Kit (Biolegend, 423106, diluted 1:500 in PBS) for 30 min at 4°C prior to Fc blocking and staining of surface antigens ...
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bioRxiv - Neuroscience 2023Quote: ... sections were immerged in a 100% formic acid for 10 min followed by a brief wash in DI H2O and PBS and an incubation with anti-Ab (800701, Biolegend – 1/500) for 24h at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in PBS and stained at 4°C with 100 µL 1:500 Zombie Aqua viability dye (BioLegend, Cat# 423102) and 1:200 mouse FC block (TruStain FcX ...
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bioRxiv - Bioengineering 2023Quote: ... The following antibodies were mixed together at 2x concentration in 50 μL and added directly to the cell suspension: BV605 CD45 (1:500, Biolegend, cat#103139), AF700 CD11b (1:300 ...
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bioRxiv - Bioengineering 2023Quote: ... The slides were then incubated in the primary antibodies (rat anti-PECAM-1, clone 390, EMD Millipore; rabbit anti-NG-2 chondroitin sulfate, EMD Millipore; rabbit anti-Keratin-14, BioLegend) diluted 1:100 in the blocking solution overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies were resuspended in 0.1% Tween-20 in PBS supplemented with 5% BSA at the following dilutions: TUJ1 (BioLegend, 801202, 1:400), SOX2 (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... for 10 min at RT then stained for the following cell surface markers for 30 min at 4°C: CD3 (T cell marker, mouse anti-CD3-BV510, BioLegend, 1:50), CD14 (monocyte marker ...
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bioRxiv - Immunology 2024Quote: ... Cells were harvested and stained for 30min at 4°C with the following antibodies: anti-CD25 BV785 (Biolegend, Cat: 356139, 1:50), anti-CD127 PE (Biolegend ...
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bioRxiv - Evolutionary Biology 2024Quote: ... or TRIM5α-HA expression (using the CytoFix/Cytoperm kit [BD] according to manufacturer’s instructions and staining with anti-HA-PE [Biolegend] diluted 1:200). Cells were gated on size (FSC vs ...
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bioRxiv - Immunology 2024Quote: ... cells were sensitized with mouse IgG2a cell-type specific antibodies (HUVEC and THP-1: CD31, HEC7, Invitrogen, 2 μg; Fibroblasts: HLA-A,B,C, W6/32, Biolegend, 2 μg) or a mouse IgG2a isotype control (MG2a-53 ...
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bioRxiv - Neuroscience 2024Quote: ... Fixation and cryostat sectioning of 3D organoids was performed as previously described.47 Immunostaining was done using antibodies to PAX6 (BioLegend, 1:200), SOX2 (Santa Cruz ...
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bioRxiv - Immunology 2021Quote: Isolated cells were stained at 4°C in FACS buffer (PBS supplemented with 2.5% bovine serum albumin) with and hematopoietic lineage markers including Ly6G (BioLegend, clone 1A8, 1:600), CD11b (BioLegend ...