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Citations for BioLegend :
8551 - 8600 of 8754 citations for Recombinant Human Interleukin 1 Beta since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... we incubated sequential de-paraffinized sections with anti-2’,3’-cyclic-nucleotide 3’-phosphodiesterase (CNPase) (mouse CNPase: 1:200 dilution; Biolegend, Inc.), anti-CD3 (rabbit ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were at 8 h post-infection and used fresh or stored at -20°C for up to 1 week and TNF was quantified using mouse TNF-α ELISA MAX kit (BioLegend) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Tyrosine hydroxylase was visualized using an Alexa Fluor® 594 conjugated mouse monoclonal anti-TH antibody at a 1:500 concentration (818004; Biolegend). Glutamine synthetase was visualized using a mouse monoclonal anti-GS antibody at a 1:100 concentration (MA5-27750 ...
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bioRxiv - Immunology 2023Quote: The following list of antibodies and their concentration against mouse proteins were employed: αCD45 (1:400; BioLegend Cat# 103147, RRID:AB 2564383), αCD3ε (1:300 ...
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bioRxiv - Cancer Biology 2023Quote: Cells were stimulated with IFNγ for 4h and the trypizinized cells were immunostained with VCAM1 antibody (1:100 dilution, BV421; BioLegend #305815). eBioscience™ Fixable Viability Dye eFluor™ 780 was used for discriminating dead cells ...
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bioRxiv - Bioengineering 2023Quote: ... Cells pre-treated with anti-mouse TfR-1 antibodies (human constant regions) were incubated with 50 nM of a second TfR-1 antibody for detection (R17, rat anti-mouse antibody; BioLegend, 113802). Cells pre-treated with anti-mouse CD98hc antibodies (human constant regions ...
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bioRxiv - Cancer Biology 2023Quote: ... single cell suspensions of samples incubated with myeloid antibody cocktail were washed and resuspended in PBS for flow cytometry run whereas lymphoid antibody cocktail-stained cells were incubated overnight with 1 mL perm buffer (Biolegend; 421402) containing 10 μL of anti-FoxP3 (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... cell suspensions were stained for 30 min at 4°C in the dark with CD45-BV605 (1:50, BioLegend #304042, RRID:AB_2562106), ITGA6-PeCy7 (1:100 ...
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bioRxiv - Immunology 2022Quote: ... 4°C) and preincubated for 15min with anti-CD16/CD32 Fc-block antibody (10μg/ml; BioLegend, San Diego, CA, 1:50) in FACS buffer followed by a 30min incubation with fluorescence dye-conjugated ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were then incubated in 1×PBST with either rabbit anti-flagellin (kindly given by Dr Monica Rolando) or mouse anti-RNAPα (BioLegend #663104) diluted 1/5,000 for 1h at room temperature with agitation ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed 3 times for 5 minutes each with 1xPBS + .03% Triton before incubating for 2 hours in secondary solution containing 2% NDS + DAPI (1:1000; Biolegend 422801) + secondary antibodies (Alexafluor 647 donkey anti-chicken ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Following incubation on ice for 30 min., cells were pelleted, washed, and resuspended in PBS/BSA containing streptavidin-APC (1:2000 dilution in PBS/BSA, (BioLegend #405207)) and incubated for 30 min on ice prior to washing ...
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bioRxiv - Immunology 2024Quote: ... in FACS buffer and stained with anti-hCD19-PE-Cy7 and anti-hCD138-PE-Cy7 (Biolegend, #302216 and #356513, 1:100). After washing ...
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bioRxiv - Cell Biology 2024Quote: ... all input solutions were supplemented with 1 μg/mL of PE-conjugated rat anti-mouse TNFα antibody (MP6-XT22, BioLegend, UK).
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bioRxiv - Immunology 2023Quote: ... 6 x 106 cells were stained with the appropriate cocktail of cell-surface conjugated antibodies in 200µL FWB with the fixable viability dye Zombie Red (BioLegend; 1:1000) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2023Quote: ... in phenol-red free DMEM-F12 in combination with live-dead stain Zombie NIR (1: 10,000 dilution; Biolegend, cat. no 423106) and incubated for 25 minutes at 37 °C and 5% CO2 conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... About 1*106 live cells were then blocked with TruStain FcXTM anti-mouse CD16/32 antibody (Clone 93; Biolegend; Cat #101320) on ice for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... and incubated in 100 μl of Cell Staining Buffer with TruStain FcX™ (anti-mouse CD16/32) Antibody (1:100; 101320, Biolegend) and True-Stain Monocyte Blocker™ (1:20 ...
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... cells from both upper and lower chambers were collected and stained with BV421-conjugated CD4 (1:100, Clone RPA-TA, BioLegend, 300501) and APC/CY7-conjugated CD8 (1:100 ...
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bioRxiv - Biochemistry 2023Quote: ... FoxP3-transduced CD4+ T cells were stained with antibodies targeting the cell surface antigens CD25 (Biolegend, Cat#102022, 1:1000 dilution) and Thy1.1 (Biolegend ...
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bioRxiv - Immunology 2024Quote: Cells were stained with 20 µL of surface antibody cocktail with Zombie NIR fixable viability kit (1:400-1000, Biolegend, 423105) in PBS for 20 minutes on ice ...
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bioRxiv - Developmental Biology 2024Quote: Single-cell suspensions were obtained at day 2.5 of differentiation Cells were first labeled with Zombie NIR fixable viability dye (BioLegend, 1:500) for the exclusion of dead cells ...
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bioRxiv - Immunology 2024Quote: The Fc receptors were blocked on 5X105 neutrophils resuspended in PBS with 5% FBS and TruStain FcX (1:50, BioLegend, 101320). Neutrophils were incubated with FITC conjugated anti-mouse Ly6G (1:50 ...
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bioRxiv - Cell Biology 2024Quote: ... The live mCherry+ or mCherry- Epcam+ miPSC or GFP+ hiPSC were sorted using the following staining: anti-Epcam antibody (1:50) (Biolegend; 118225). Cells were loaded on a 10x Genomics Chromium controller.
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bioRxiv - Microbiology 2024Quote: ... each sample was counterstained with the desired concentration (0.5ul/1 × 106 cells) of 7AAD viability staining solution (Biolegend; Cat. No. 420404) before analysis ...
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bioRxiv - Developmental Biology 2024Quote: ... or with an anti-integrin ß4 antibody coupled to R-phycoerythrin (ITGß4-PE, 1:500, clone 58XB4, BioLegend, San Diego CA) for isolation of CTB ...
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bioRxiv - Cancer Biology 2024Quote: ... single cells isolated from tissue were labeled for 30 min at room temperature with Alexa Fluor 647-conjugated anti-EpCAM (1:50, Biolegend 324212), PE-conjugated anti-CD49f (1:100 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were pelleted by centrifugation (2,000 rpm 5 min) and then treated with 1 mL 1X red blood cell (RBC) Lysis Buffer (Biolegend; cat# NC9067514) to lyse red blood cells ...
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bioRxiv - Cell Biology 2024Quote: ... Cell pellets were gently resuspended in 100 µl FACS buffer and stained with antibodies (CD38-APC, Clone: HIT2 (Biolegend, 1:100), CD45RA-PE-Cy7 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were permeabilized for intra-cellular staining with a saponin-based buffer (1% saponin, 10% BSA in PBS) and stained with an antibody for Cytochrome C AlexaFluor 647 (Biolegend, 612310) used at 1:2000 dilution and DAPI ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then stained with the following conjugated cell-surface marker antibodies at 1:50 dilutions: CD326 (EpCAM) PE (Biolegend, 324206) and CD45 BV786 (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were permeabilized for intra-cellular staining with a saponin-based buffer (1% saponin, 10% BSA in PBS) and stained with an antibody for Cytochrome C AlexaFluor 647 (Biolegend, 612310) used at 1:2000 dilution and DAPI ...
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bioRxiv - Neuroscience 2024Quote: ... 0.3% triton in PBS) for 3 hours then incubated with Alexa Fluor 594 anti-β-amyloid (Clone 6E10; 1:1,000; BioLegend; 803019), Olig2 (1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were washed with DPBS three times and stained with 1 µg/mL Streptavidin-Alexa Fluor 647 (AF647) (BioLegend, #405237) for another 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated for 25 m on ice in a cocktail of fluorochrome-conjugated antibodies (B220 (RA3-6B2-BV605; 1:200; BioLegend #103244), TCRβ (H57-597-PE-Cy7 ...
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bioRxiv - Immunology 2024Quote: ... diluted 1:500 in 1x Permeabilisation buffer with 5% Normal Donkey Serum at +40C and 1 h staining with DyLightTM 649 Donkey anti-rabbit IgG (BioLegend, #406406) diluted 1:500 in 1x Permeabilisation buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Around 900 μl of plasma from each mouse was used to stain for T cell (1:150, APC-CD3e/clone 145-2C11, Biolegend Cat. 100312), B cell (1:300 ...
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bioRxiv - Immunology 2021Quote: ... clone: NKI-SAM-1), αL (CD11a, clone: HI111), αM (CD11b, clone: M1/70), αX (CD11c, clone: 3.9) (all Biolegend, San Diego, CA), or α9 (CD49i ...
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bioRxiv - Immunology 2021Quote: ... five groups of cells were pre-labelled with mouse HashTag oligos (HTO) on cell surface: spleen T cell control group (SPT ctrl) with hashtag 1 (BioLegend, Cat# 155801); spleen T cell IL-1β/IL-23 stimulation group (SPT stim ...
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bioRxiv - Immunology 2019Quote: ... against intracellular antigens of interest were added and incubated for 90 minutes at 4°C (IFNγ: PE-Cy7, clone XMG1.2; IL2: PE, clone JES6-5H4; TNFα: APC, clone MP6-XT22; all Biolegend. all 1:200). Next ...
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bioRxiv - Immunology 2019Quote: PBMCs were seeded in a 24 well plate at 2 × 106 cells/well in RPMI-1640 with anti-CD107a antibody (Biolegend, 1:200). Then ...
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bioRxiv - Neuroscience 2021Quote: Nrxn1 neuron cKO-T brain slices (coronal and sagittal) were labeled for Nrxn1 (Fig. 1c-d) using purified HA-647 (mouse, 1:500, Biolegend, Cat# 682404) as described above ...
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bioRxiv - Biophysics 2020Quote: The antibodies used for these experiments were mouse purified anti-HA.11 Epitope Tag monoclonal (clone 16B12) (Biolegend 901502; 1:500 dilution), rabbit affinity-isolated anti-FLAG polyclonal (Sigma-Aldrich F7425 ...
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bioRxiv - Biochemistry 2020Quote: ... Cells at a density of 2×107 cells/mL were incubated for 1 hour in flow cytometry buffer with 5 μL of Pacific Blue™ conjugated anti-CD235a antibody (BioLegend #349108) and 4 μg of unconjugated mouse IgG2a (Merck #M5409 ...
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bioRxiv - Systems Biology 2021Quote: Cells were stained overnight at 4°C in blocking solution with the following antibodies: AlexaFluor® 488 anti-Vimentin (1:1000, Biolegend, #677809), AlexaFluor® E-cadherin (1:200 ...
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bioRxiv - Cancer Biology 2020Quote: ... catalog number L100-L115-01) in PBS with 1% BSA and incubated the beads with 3.5 µg/ml biotinylated anti-CD81 antibody (BioLegend, clone Eat-2) at the room temperature for 40 minutes ...
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bioRxiv - Cell Biology 2022Quote: Whole lung single cell suspensions were prepared as above and then blocked in SB containing 1:50 TruStain FcX™ (anti-mouse CD16/32) Antibody (BioLegend, #101319) for 10 min at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 5×105 cells were stained for 30 min using the Zombie Aqua fixable viability dye (BioLegend Cat# 423101, 1:500 dilution), then fixed for 1 h in Foxp3/Transcription Factor Fixation/Permeabilisation Solution (ThermoFisher Cat# 00552300) ...
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bioRxiv - Molecular Biology 2022Quote: ... samples P008-016 and P048-119 (Suppl. Fig. 4D) were labelled with TotalSeq B Hashtag antibodies 1-4 (Biolegend, clone LNH-94) during the staining procedure described above and pooled in groups of four.
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bioRxiv - Immunology 2020Quote: ... OVA and HEL were biotinylated in house to ∼1 biotin per antigen molecule and tetramers constructed as described [14] using streptavidin-PE (Biolegend, CA, USA), streptavidin-APC (Biolegend ...