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Citations for BioLegend :
801 - 850 of 1059 citations for Zinc Finger Protein 50 ZNF50 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Wells were again washed 3 times before adding 50 µL per well of HRP Streptavidin (#405210, BioLegend, USA) diluted 1:2000 in 1% SureBlock solution and incubated 1 h at RT ...
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bioRxiv - Immunology 2024Quote: ... sections were stained for 3h at room temperature with anti-CD3 PE-Dazzle594 (Biolegend, Cat: 100245, 1:50), anti-CD4 PE (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... and 1:20 dilution mouse anti-human CD27-PE (stock 50 ug/mL, BioLegend, San Diego, CA, USA), which has been reported as a marker for juvenile B cells ...
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bioRxiv - Immunology 2024Quote: ... TOYOBO) supplemented with 50 μg/mL of FITC anti-mouse I-A/I-E (clone M5/114.15.2, BioLegend) for 1 h at room temperature in the dark following pretreatment with Ultra-LEAF purified anti-mouse CD16/32 Ab (clone 93 ...
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bioRxiv - Immunology 2024Quote: ... Single cell suspensions were washed in PBS and then incubated with 50 μl Zombie Aqua viability dye (BioLegend) for 10 minutes at room temperature in the dark ...
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bioRxiv - Immunology 2024Quote: ... all cells were blocked with 1:50 dilution of TruStain FcX anti-mouse CD16/CD32 (Clone 93, Biolegend) and Fixable Viability Dye (eBioscience ...
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bioRxiv - Immunology 2020Quote: ... Recombinant mouse (rm) M-CSF and GM-CSF proteins were obtained from BioLegend. MOG35-55 peptide (MEVGWYRSPFSRVVHLYRNGK ...
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bioRxiv - Systems Biology 2021Quote: ... surface proteins were stained with a cocktail of 53 TotalSeq-C antibodies (Biolegend). Antibody concentrations were either predetermined by titration (Kotliarov et al. ...
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bioRxiv - Immunology 2022Quote: ... Antibodies with specificity for the following cell surface proteins were purchased from Biolegend with different fluorochrome labels ...
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bioRxiv - Microbiology 2020Quote: ... Protein G agorose beads that were pre-adsorbed to either anti-HA (BioLegend) or anti-FLAG (Millipore ...
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bioRxiv - Immunology 2022Quote: ... CD46 APC (targets MCP protein) clone TLA-2-10 (cat# 352405, BioLegend®), CD49d APC (targets α4 integrins ...
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bioRxiv - Immunology 2022Quote: ... Protein concentrations of inflammatory cytokines were determined by LegendPlex™ Multiplex Assay (BioLegend) using the pre-defined mouse inflammation panel according to the manufactureŕs instructions ...
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bioRxiv - Immunology 2019Quote: ... Type III IFN proteins were measured using a custom-designed LEGENDplex kit (BioLegend). IFN-β was measured using the Verikine ELISA (PBL) ...
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bioRxiv - Immunology 2021Quote: ... and cells were stained with antibodies against surface proteins CD3 (APC-fire, BioLegend), CD11b (APC Cy7 ...
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bioRxiv - Immunology 2022Quote: ... Antibodies to detect immunoprecipitated proteins were as follows: Eos (1:500, Biolegend, W15169A), STAT5 (1:5000 ...
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bioRxiv - Immunology 2023Quote: ... whereas ADT libraries (cell surface protein libraries) were processed following instructions from Biolegend. 3’ gene expression and ADT libraries were quantified by fragment analyzer and pooled for multiplexed sequencing on a NovaSeq 6000 instrument by the Deep-sequencing Facility at the Max Planck Institute for Immunobiology and Epigenetics ...
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bioRxiv - Immunology 2024Quote: ... biotinylated gE proteins (Acro Biosystems) were conjugated with BV421- or APC-streptavidin (Biolegend) at a molar ratio of 4:1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Following two washes with PBS (centrifuged at 700 x g for 5 minutes), cells were stained in 200 μL PI staining solution (0.1% Triton X-100, 50 μg propidium iodide (BioLegend), 0.2 mg/mL RNase ...
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with the CD45-PerCPCy5.5 antibody (1:50) (Biolegend) and the EpCAM-FITC antibody (G8.8 clone ...
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with a cocktail of fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50) (Biolegend), Ly51-PE (1:800 ...
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bioRxiv - Genomics 2019Quote: ... The resuspended cells were incubated for 20 min at 4 °C with the fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50) (Biolegend), Ly51-PE (1:800 ...
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bioRxiv - Immunology 2022Quote: ... neutrophils were incubated with autologous activated platelets (1:10 ratio) (65) for 2.5h and human recombinant CCL28 (50 nM) (BioLegend), the CCR3 antagonist SB328437 (10 mM ...
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bioRxiv - Immunology 2022Quote: ... and anti-CD49a APC (1:50; clone HMα1) and anti-CD11b BV570 (1:100; clone M1/70) from BioLegend. Cells were fixed for 30 min at 4°C using eBioscicencesTM FoxP3/Transcription factor staining buffer set (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... The cells were differentiated into macrophages by incubation with 50 ng/ml macrophage colony–stimulating factor (M-CSF) (BioLegend) in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were: Syn303 [a mouse monoclonal antibody that recognizes residues 1-5 of human α-synuclein (50)] (BioLegend) at 1:4,000 ...
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bioRxiv - Immunology 2019Quote: ... The cell pellet was then resuspended in 50µl (1/50 dilution in FACS buffer) of TruStain FcX blocker antibody (BioLegend) and incubated at 4°C for 10minutes ...
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bioRxiv - Cell Biology 2021Quote: ... and 50 μl of conditioned complete medium containing primary antibody at concentrations of 5 μg/ml for CD59 (Biolegend) or 10 μg/ml Alexa Fluor 488–linked transferrin (Molecular Probes ...
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used for intracellular staining: Anti-human TBET FITC (1:50, clone 4B10, BioLegend, cat # 644812), Anti-human Granzyme B FITC (1:100 ...
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Bacterial Polyphosphates Induce CXCL4 and Synergize with Complement Anaphylatoxin C5a in Lung InjurybioRxiv - Immunology 2022Quote: ... preincubated for 20 min with anti-mouse CD16/CD32 antibody (TruStain FcX, BioLegend, San Diego, CA, USA, 1:50) in FACS buffer followed by 20 min incubation with fluorescent dye-conjugated ...
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bioRxiv - Immunology 2022Quote: ... samples were resuspended in 180 μl FACS buffer after which 50 μl precision count beads (Biolegend, San Diego, CA) were added to BAL and NT samples ...
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bioRxiv - Cancer Biology 2023Quote: ... were coated with 50 µL per well of Purified anti-human IFNγ Antibody (clone MD-1; #507502, BioLegend, USA) diluted 1:200 in 1X PBS (#5460-0023 ...
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bioRxiv - Immunology 2023Quote: ... The CD16/CD32 receptors were then blocked by incubating with a 1/50 dilution of TruStain FcX™ (BioLegend) in PBS at room temperature for 15 min ...
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bioRxiv - Immunology 2023Quote: ... 1:300), NK1.1 (108736, 1:150), Tbet (644810, 1:50), TCRβ (109243, 1:100), TER119 (116228, 1:200) from Biolegend, CD19 (45-0193-82 ...
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bioRxiv - Synthetic Biology 2023Quote: ... resuspended in 50 µl LIVE/DEAD staining solution (PBS with 1:500 Zombie Violet Dye, Biolegend cat. no. 423113) and incubated for 20 min at 4 °C in the dark ...
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bioRxiv - Cancer Biology 2023Quote: ... immunohistochemistry (IHC) and flow-cytometry (FC): Pacific Blue anti-mouse Ly-6A/E (Biolegend, Cat#108120, 1:50 FC), Sca-1/Ly6A/E (E13 161-7 ...
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bioRxiv - Cell Biology 2023Quote: ... an RBD - HC2.RBD.rFC (Metafora – 1:5) was used in conjunction with Alexa 647 donkey anti rabbit Poly4064 (1:50 Biolegend).
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed with ice cold PBS and surface stain with primary anti-PD-L1 (1:50) antibody (BioLegend) on ice for 90 min ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was resuspended in 50 µl of the Annexin V Apoptosis Detection Kit/7-AAD solution (BioLegend) and incubated for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... T lymphocytes were evaluated using the following antibodies: rat anti-mouse IL-17A (TC11-18H10.1, 1/50; Biolegend, #506922), rat anti-mouse RORγt (AFKJS-9 ...
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bioRxiv - Immunology 2024Quote: ... cells were harvested and stained for 30min at 4°C with anti-CD25 APC (Biolegend, Cat: 102012, 1:50), and ZombieUV (Biolegend ...
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bioRxiv - Microbiology 2022Quote: ... Endotoxin-free recombinant mouse IL-1α and IL-1β proteins were purchased from BioLegend.
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bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal anti-NPC proteins (FXFG repeat-containing nucleoporins) (1:1,000; mAb414; 902907, Biolegend), rabbit monoclonal anti-BANF1/BAF (1:200 ...
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bioRxiv - Immunology 2022Quote: ... Briefly PBMC or IDCs were cultured in presence of a protein transport inhibitor (Biolegend) in media alone (control ...
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bioRxiv - Cancer Biology 2019Quote: ... Biotinylated proteins were detected either by Alexa Fluor®594 Streptavidin (405240, Biolegend®) or Streptavidin-HRP (21130 ...
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bioRxiv - Immunology 2022Quote: ... Cells were than stained with Siglec-5 Fc chimeric protein (Biolegend, San Diego, CA) pre-incubated with anti-human IgG Fc fluorescent antibody (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Antibodies with specificity for the following intracellular and intranuclear proteins were purchased from Biolegend with different fluorochrome labels ...
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bioRxiv - Genomics 2019Quote: ... or CC2C6 (for pyro-Glu modified protein; BioLegend, Cat. #323123/4, RRID: AB_2716202/3) anti-CD47 antibodies were used with Protein-G DynabeadsTM or Biotin-binder DynabeadsTM ...
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bioRxiv - Immunology 2021Quote: ... and mouse anti-β-tubulin (1:1000; BioLegend; used as a control loading protein). Membranes were then incubated with appropriate secondary antibody (1:5000 ...
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bioRxiv - Immunology 2021Quote: ... CD28-peridinin chlorophyll protein (PerCP) Cy5.5 (clone CD28.2; Cat#302922, BioLegend, San Diego, CA), CD95-fluorescein isothiocyanate (FITC ...
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bioRxiv - Cell Biology 2020Quote: ... Anti-fibroblast specific protein (FSP1, displayed as S100A4; Cat: 370003) was purchased from Biolegend, CA ...