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Citations for BioLegend :
751 - 800 of 1059 citations for Zinc Finger Protein 50 ZNF50 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The following antibodies and dilutions were used: anti-mouse CD11b-APC (BioLegend, 101212, dilution 1:50), anti-mouse CD169-AlexaFluor488 (BioLegend ...
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bioRxiv - Immunology 2023Quote: Extracellular staining was performed using the following antibodies: anti-human CD3ε BV785 (1:50, OKT3, BioLegend), anti-human CD19 BV605 (1:20 ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were allophycocyanin (APC)-conjugated monoclonal rat anti-mouse IgG1 RMG1–1 (Biolegend 1:50) or goat anti mouse IgG poly4053 (Biolegend 1:50) ...
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bioRxiv - Microbiology 2023Quote: ... The cells were differentiated into macrophages with 50 ng/ml macrophage colony–stimulating factor (#576404, BioLegend) and maintained by incubation in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Cancer Biology 2023Quote: ... single cell suspensions were labelled with TotalSeqA anti-human hashtag oligonucleotide (HTO) antibodies (Biolegend, 1:50) to allow downstream de-multiplexing in silico as previously described9 ...
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bioRxiv - Pathology 2024Quote: ... , and 1.25μL Cd34- PE/CF594 (Cat# BDB562449) and 3μL Cd31-AF700 (Biolegend Cat# 50-207-2950), for 30 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... 1 mL 50 mg/mL APC-mouse-anti-human CD56 (BioLegend; Cat. No. 318310; clone HCD56), 0.5 mL 200 mg/mL APC/Cy7-mouse-anti-human CD3 (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... Protein detection using anti-HA (Covance 16B12, BioLegend, Dedham, MA, USA) or antiactin antibodies (Cell Signaling ...
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bioRxiv - Bioengineering 2021Quote: ... expanded and screened for IGF2R protein by anti-IGF2R antibody (Biolegend) by flow cytometry.
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bioRxiv - Cancer Biology 2022Quote: Protein expression measurements were performed using TotalSeq-A reagents from Biolegend according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Bound biotinylated protein was detected by adding Avidin-HRP (405103, BioLegend) diluted 1:2000 in TSM buffer ...
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bioRxiv - Immunology 2020Quote: ... Bound proteins were detected by staining with fluorescently conjugated antibodies (BioLegend) and measured by flow cytometry ...
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bioRxiv - Biochemistry 2021Quote: ... USA)-conjugated anti-mouse CD206 and activated protein C (APC, BioLegend, USA)-conjugated anti-mouse CD86 at room temperature for 1 h without light ...
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bioRxiv - Genomics 2022Quote: Antibody-derived DNA tags for membrane proteins were purchased from Biolegend and listed in Table S5 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins on blots were detected using anti-RpoB (Biolegend; 663905; AB_2566583), anti-RecA (Pocono Rabbit Farm & Laboratory ...
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TNFR1/p38αMAPK signaling in Nex+ supraspinal neurons regulates sex-specific chronic neuropathic painbioRxiv - Neuroscience 2023Quote: ... p38 mitogen-activated protein kinase (p38MAPK, mouse, 1:2000, Biolegend 602651), and phosphorylated-p38MAPK (phospho-p38MAPK ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were treated with TNF-α recombinant human protein (Biolegend) at a concentration of 75 ng/ml for 24 h in combination with cycloheximide (CHX ...
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bioRxiv - Synthetic Biology 2023Quote: ... Secreted proteins were measured using the ELISA MAX Deluxe kits (Biolegend). Absorbance was measured at 450 nm and 570 nm using a Synergy H1 plate reader (BioTek) ...
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bioRxiv - Immunology 2024Quote: ... peridinin chlorophyll protein-cyanin 5.5 (PerCP-Cy5.5)-anti-CD16 (3G8; BioLegend), Pacific Blue-anti-CD45 (HI30 ...
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bioRxiv - Neuroscience 2022Quote: ... Pre-centrifuged 50 μL of hashtag antibody solution containing 2 uL of TotalSeq-A Hashtag antibody (BioLegend), with a unique barcode sequence for each iPS cell line ...
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bioRxiv - Immunology 2020Quote: ... Intracellular antibodies were added during permeabilization and included 1:50 each of: anti-CD3 AlexaFluor700 (UCHT1, BioLegend), anti-IFN-γ PE-Dazzle594 (4S.B3 ...
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bioRxiv - Cell Biology 2023Quote: ... Alexa-Fluor®-488 SSEA1 (CD15) (1:50) and Alexa-Fluor®-647 SSEA5 (1:200) (BioLegend). In all experiments ...
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bioRxiv - Immunology 2023Quote: ... rhIL-2 (50 Uml-1, Novartis) and anti-mIFN-γ (5 µg ml-1, clone XMG1.2, Biolegend) in the presence or absence of 30 mM NaCl ...
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bioRxiv - Immunology 2024Quote: ... 1 mL 50 mg/mL PE/Dazzle594-mouse-anti-human CD16 (BioLegend; Cat. No. 302054; clone 3G8), 1 mL 100 mg/mL PE/Cy7-mouse-anti-human CD39 (BioLegend ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-dephosphorylated neurofilament protein (clone smi32; Biolegend, San Diego, CA, USA); rabbit anti-Caspr1 (clone EPR7828 ...
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bioRxiv - Biochemistry 2022Quote: ... or 0.5ng/mL of Wnt3a recombinant protein (BioLegend, San Diego, CA, #772301). Luciferase activity was measured by bioluminescence (Promega Dual-Glo Luciferase Assay System ...
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bioRxiv - Immunology 2022Quote: ... −phycoerythrin (PE) and −peridinin chlorophyll protein complex (PerCP) were purchased from Biolegend. Phospho-specific mAbs against p65 (S536 ...
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bioRxiv - Microbiology 2020Quote: ... antibodies against the following proteins and epitopes were used: HA (16B12, BioLegend), β-actin (AC-74 ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated protein was then mixed with streptavidin fluorophores (AF647, BioLegend; BV421, BioLegend), as described previously6 ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated protein was then mixed with streptavidin fluorophores (AF647, BioLegend; BV421, BioLegend), as described previously6 ...
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bioRxiv - Genomics 2022Quote: ... Proteins of interest were visualized using an anti-Flag-PE (BioLegend, 637310), an anti-HA-Alexa647 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... WT biotinylated RBD protein conjugated with TotalSeq™-C0973 Streptavidin (BioLegend, 405275) and TotalSeq™-C0974 Streptavidin (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... 1X protein transport inhibitor (Fisher Healthcare) and 1:1000 PMA/Ionomycin (Biolegend) were added ...
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bioRxiv - Bioengineering 2024Quote: The scFc protein was labeled with SNAP-Surface 488 (BG-488, Biolegend). In brief ...
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bioRxiv - Immunology 2022Quote: ... 1:50), anti-CD45.1 (APC, clone A20, 1:100), anti-C45.2 (FITC, clone 104, 1:100) from BioLegend.
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bioRxiv - Immunology 2021Quote: ... at room temperature and resuspended in 50 μL ICS antibody cocktail within Permeabilization Wash Buffer (1x) (PWB) (BioLegend) for 30 mins in the dark at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated with a mouse anti-human C3 APC conjugated antibody (1:50; Biolegend, 846106, clone 3E7iC3b) and mouse anti-Myc Alexa488 conjugated antibody (1:50 ...
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bioRxiv - Immunology 2020Quote: ... #SK-4105) and others were also incubated with an AF647 anti-neutrophil antibody (1:50, Biolegend UK #127609). Slides were scanned on a ZEISS Axio Scan.Z1 slide scanner.
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bioRxiv - Cell Biology 2019Quote: Panel 3 (fresh in vivo tissue): anti-CD45-Brilliant Violet 570 (HI30 clone, 304034, BioLegend, dilution 1:50), anti-CD235a-Pacific Blue (HI264 clone ...
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bioRxiv - Immunology 2020Quote: ... 1:50 of anti-CD8 (FITC, RPA-T8) and 1:25 of anti-CD26 PE-Cy7 (BA5b, BioLegend), as well as IFN-γ ELISPOT assay to confirm MAIT cell identify and clonal purity.
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bioRxiv - Cell Biology 2021Quote: ... 50 μl of blood was resuspended in 1 ml of red blood cell (RBC) lysis buffer (Biolegend™) for 10 min on ice followed by two washes with 1 ml phosphate buffer saline (PBS) ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.02% sodium azide) and resuspended in 25 µL 1:50 mouse FC block (TruStain FcX, BioLegend Cat# 101320), and left on ice for 5 minutes ...
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bioRxiv - Immunology 2020Quote: ... and CV05) were stained with a divergent set of antibodies: CD19-PE 1:50 (clone HIB19, BioLegend, 302207), CD38-PEcy7 1:50 (clone HIT2 ...
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bioRxiv - Immunology 2020Quote: ... 50 µL Intracellular Staining Perm Wash Buffer supplemented with 5 µL of IL-10-BV421 antibody (Biolegend, 501421) was added for an additional 20 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation with 50 ng/mL anti-Fc-γ III/II (CD16/32) receptor Ab (93, BioLegend) in 100 μl of FACS Buffer (1% FBS and 1 mM EDTA in PBS) ...
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bioRxiv - Immunology 2022Quote: ... culture media was removed and replaced with media containing either 50 ng/mL interleukin 4 (IL-4) (BioLegend) for 48 hr to generate M2 macrophages or 50 ng/mL interferon-gamma (IFN-γ ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated O/N in 50% block solution with anti-Prox1 1:500 (Biolegend, San Diego, CA; Rb), anti-ChAT 1:1K (Chemicon ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then stained with 2.5 μl of PE anti-human E-cadherin (50 μg/ml; Biolegend, 324106) or matched concentration of PE Mouse IgG1 ...
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bioRxiv - Microbiology 2022Quote: ... Cell viability was determined using Zombie-NIR Fixable Viability Dye (0.4 μL per 50 μL staining volume; Biolegend). Antibodies used for surface staining were as follows ...
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bioRxiv - Immunology 2023Quote: ... Cells were next resuspended in 50 µl (per mouse) lavage buffer containing 0.5 µg biotinylated anti-mouse FcεRIα (clone MAR-1; Biolegend) and incubated 20 minutes at 4°C ...