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Citations for BioLegend :
801 - 850 of 863 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 500,000 cells were resuspended in PBS + 1% FBS and spun at 300g for 10 minutes at 4°C and then resuspended in 25 μL staining buffer (BioLegend #420201). 2.5 μL human TruStain FcX (BioLegend #42230 ...
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bioRxiv - Immunology 2023Quote: ... For the memory B cell EliSpot, PBMC were stimulated with 1 µg/ml R848 (SigmaAldrich, SML0196) and 10 ng/ml recombinant human IL-2 (Biolegend, 791904) for 5 days before harvesting and plating of 50-100.000 cells/well ...
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bioRxiv - Bioengineering 2023Quote: ... Cells pre-treated with anti-mouse CD98hc antibodies (human constant regions) were incubated with 10 nM of a second CD98hc antibody for detection (RL388, rat anti-mouse antibody; BioLegend, 128202). Non-treated cells were also incubated with the TfR-1 (R17 ...
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bioRxiv - Immunology 2023Quote: ... stained for 10 minutes at room temperature with Ghost Dye Violet 510 and Fc receptor blocking solution (Human TruStain FcX™, BioLegend), and washed once in FACS buffer ...
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bioRxiv - Immunology 2024Quote: Production of IFNγ and IL-10 from T cell culture supernatant was measured using Legend Max ELISA Kit Mouse IFNγ (BioLegend, 430807) and Mouse IL-10 (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... Stimulated PBMCs were maintained in RPMI-1640 supplemented media with 100 ng/ml CD3 and 10 ng/ml recombinant human IL-2 (Biolegend, 589104).
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Langerhans cells regulate immunity in adulthood by regulating postnatal dermal lymphatic developmentbioRxiv - Immunology 2024Quote: ... The media was changed with new serum free media and incubated with his tagged recombinant mouse VEGF-C (10 μg/mL) for 30 min before staining in PBS with anti-his PE (1:100, BioLegend, #362603) and cell surface markers.
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bioRxiv - Immunology 2024Quote: ... Cells were stained with Zombie NIR viability dye for 10-15 minutes at room temperature (Biolegend, Cat. 423106, 1:1000 dilution), followed by quenching and washing with FACS buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were permeabilized for intra-cellular staining with a saponin-based buffer (1% saponin, 10% BSA in PBS) and stained with an antibody for Cytochrome C AlexaFluor 647 (Biolegend, 612310) used at 1:2000 dilution and DAPI ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were permeabilized for intra-cellular staining with a saponin-based buffer (1% saponin, 10% BSA in PBS) and stained with an antibody for Cytochrome C AlexaFluor 647 (Biolegend, 612310) used at 1:2000 dilution and DAPI ...
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bioRxiv - Molecular Biology 2024Quote: ... and resuspended in a cocktail of FACS buffer with anti-FCR (made in house; 1:10) and fluorochrome-conjugated antibodies against B220 (RA3-6B2-BV605; 1:200; BioLegend #103244), TCRβ (H57-597-PE-Cy7 ...
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bioRxiv - Cancer Biology 2024Quote: ... Half of the medium was removed on day 2 and replenished with warmed complete RPMI medium with 40 ng/ml GM-CSF and 10 ng/ml IL-4 (Biolegend, 574304). The culture medium was entirely changed on day 3 and replaced by fresh warmed medium with 20 ng/ml GM-CSF and 5 ng/ml IL-4 ...
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bioRxiv - Cell Biology 2024Quote: ... were thawed in a 37°C water bath for 2 mins and resuspended in 10 ml of warm Staining Buffer (SB; Biolegend #420201). Cells were washed twice at 300 g for 10 mins ...
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bioRxiv - Cell Biology 2024Quote: ... were thawed in a 37°C water bath for 2 mins and resuspended in 10 ml of warm staining buffer (SB; Biolegend #420201) or media buffer (Thermo Fisher Scientific DMEM #12634010 or RPMI #61870010 ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were incubated for 10 min at RT in 1.0 μg anti-mouse CD16/32 antibody (BioLegend, San Diego, CA, USA) containing 500 μl FACS buffer for Fc receptor blocking ...
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bioRxiv - Microbiology 2020Quote: ... approximately 2-10 million total human lung cells were resuspended in MACS buffer and blocked with Human TruStain FcX (Biolegend cat# 422032) for 15min at 4°C followed by HTII-280 antibody (Terrace Biotech ...
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bioRxiv - Microbiology 2021Quote: ... were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) in the presence of GM-CSF (20 ng/ml, BioLegend, Cat# 576304) for 9-12 days as described in (Dai et al. ...
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bioRxiv - Microbiology 2021Quote: ... To assess ligands-receptor specificity to this interaction we performed these assays using anti-Cd11b antibody (Biolegend Cat # 101211, 10 μl/ml) and a non-PfEMP1-blocking anti-ICAM-1 antibody (Biolegend Cat # 322702 ...
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bioRxiv - Cell Biology 2021Quote: ... and then resuspended in 10% FCS in PBS (100μl/well) containing TruStain FcX™ (anti-mouse CD16/32) antibody (Biolegend; 1:200) and incubated on ice for 20mins ...
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bioRxiv - Cell Biology 2021Quote: ... The fixed cells were permeabilized with 0.1% Triton-X 100 for 10 min at RT and then incubated with chicken anti-vimentin (1:200, 919101, Biolegend, CA, USA) for 30 min in phosphate buffered saline (PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μL of CytoC stain buffer (2% Tween20, 10% BSA (w/v) in PBS) + 1:400 Alexa Fluor 647 anti-cytochrome c antibody (Biolegend, cat#612310) + 1:100 DAPI (1 mg/mL ...
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bioRxiv - Immunology 2020Quote: ... Stamps were then incubated for 1 hour with either 1) 10 μg/mL antibodies against LFA-1 (αLFA-1; BioLegend, clone HI111) or 2 ...
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bioRxiv - Immunology 2020Quote: ... cytokine staining mixture (containing anti-IFN-γ, anti-IL-2, anti-IL-4, anti-IL-10 and anti-TNF-α antibodies, Biolegend) was added to each well ...
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bioRxiv - Microbiology 2023Quote: ... Nonspecific antigen binding to Fc receptors was inhibited via 10 minutes of blocking with Human TruStain FcX (BioLegend, San Diego, CA, USA). Cells were subsequently stained with mouse anti-human CD20 (Alexa-700) ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were centrifuged at 300×g for 5 min and washed with 10 mL cell staining buffer (#420201, Biolegend, San Diego, CA). Human FLRT2 antibody (#AF2877 ...
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bioRxiv - Immunology 2023Quote: Cytokine production was quantified from cell supernatants or serum using the following ELISA kits: ELISA MAX Deluxe Set Mouse IL-10 (BioLegend, Cat# 431414), ELISA MAX Deluxe Set Human IL-10 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... IL-1b, IL-6, IL-10, IL-17A, IL-12p70, GM-CSF, IL-23, IFN-b, MCP-1, IL-27; BioLegend Cat# 740446), Mouse TH Panel v4 (TNF-α ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in 90 μl of 1% BSA in PBS and Fc blocked with 10 μl Human Trustain FcX (Biolegend cat. 422302) for 10 minutes on ice then stained on ice for 45 minutes with a pool of 268 antibodies in 100 μl ...
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bioRxiv - Neuroscience 2023Quote: ... sections were immerged in a 100% formic acid for 10 min followed by a brief wash in DI H2O and PBS and an incubation with anti-Ab (800701, Biolegend – 1/500) for 24h at 4°C ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated for the period of activation expansion with 20µg/ml of anti IL-10 antibody (JES5-2A5; Biolegend catalog No. 504908) or corresponding isotype control (Biolegend catalog No ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and incubated in FACS buffer (1xPBS, 2%FCS) with the following anti-human antibodies: CD19-BV785 (BioLegend, 1:10), CD3-APC-eFluor 780 (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were then plated as described above and human IL-10 was measured in the cell supernatants by ELISA (Biolegend, Cat. # 430604).
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bioRxiv - Biochemistry 2023Quote: ... The fixed cells were permeabilized with 0.1% Triton-X 100 for 10 min at RT and then incubated with chicken anti-vimentin (1:200, 919101, Biolegend, CA, USA) for 30 min in phosphate buffered saline (PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Then cells were fixed with 4 % methanol-free formaldehyde in HBSS for 10 min prior to staining with either 4 μg/ml anti-ACE2 (Biolegend, clone A20069I) or anti-NRP-1 (Biolegend ...
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Most L1CAM is not associated with extracellular vesicles in human biofluids and iPSC–derived neuronsbioRxiv - Neuroscience 2024Quote: ... EBs were plated on Geltrex™-coated 6-well plates (10 EBs/well) in APEL™2 medium supplemented with VEGF (40 ng/ml) (BioLegend), SCF (50 ng/ml ...
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bioRxiv - Immunology 2024Quote: Cells were incubated for 10 mins with Fc block (homemade containing 24G2 supernatant and mouse serum) surface stained with anti-CD45 FITC (Biolegend, 30-F11) or anti-CD45-PE (BD ...
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bioRxiv - Cell Biology 2024Quote: ... were each sorted in separate 1.5 ml tubes and labelled with 0.05 μg of oligo-hashed antibodies specific for MHC class I and CD45 (BioLegend, TotalSeq B1-10) for 30 minutes on ice before being washed three times with HBSS containing 2% FBS ...
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bioRxiv - Immunology 2021Quote: ... Nonspecific binding was blocked by incubation for 10 min at 4 °C with anti-CD16/CD32 antibody (Biolegend, clone 93, 5 ng/ μL) antibody and stained with the appropriate antibodies for 15 min at 4 °C ...
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bioRxiv - Bioengineering 2021Quote: ... 1×106 P14 or pmel splenocytes were co-stained with Db-GP33 liposomes (10 μg/ml) and anti-mouse CD8-PE (Biolegend, Clone: 53-6.7) for 30 min on ice ...
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bioRxiv - Microbiology 2021Quote: ... approximately 200,000 cells per sample were resuspended in staining buffer (PBS, 2% BSA, 0.01% Tween) and incubated for 10 minutes with Fc block (TruStain FcX, Biolegend; FcR blocking reagent, Miltenyi). Cells were then incubated with barcoded hashing antibodies for 30 min at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... anti-neurofilament to a phosphorylated heavy chain neurofilament (NF-H; mouse anti-human monoclonal antibody SMI-34, dilution 1:100, epitope retrieval time 10 min, Biolegend 835503; Biolegend, San Diego, CA). Hemosiderin deposition was evaluated on a section with only counterstain ...
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bioRxiv - Immunology 2021Quote: BMDMs were generated by culturing total BM cells for 7 days in complete RPMI supplemented with 10 ng/mL recombinant mouse M-CSF (BioLegend, Cat# 576406M-CSF). C8-S cells (ATCC ...
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bioRxiv - Immunology 2023Quote: Purified and biotinylated IL-6 (504502 and 504601, respectively) and IL-10 (505002 and 504906, respectively) antibodies were purchased from Biolegend (San Diego, CA). Purified antibodies were used to coat an ELISA plate ...
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bioRxiv - Microbiology 2023Quote: ... Samples were stained immediately after fixation with Alexa Fluor 647-labeled mouse-anti-human CD56 (10 μg/mL, Biolegend, San Diego, CA, USA) for 1 h and washed with PBS (Gibco ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed in PBS and then stained for 10 minutes at room temperature with Zombie Aqua fixable viability dye (Biolegend, San Diego, CA) for dead cell exclusion ...
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bioRxiv - Developmental Biology 2023Quote: ... 10 mg of protein G agarose beads were crosslinked to 5 μL of mouse anti-HA antibody (BioLegend, 901501, 1:1000, RRID: AB_2565006) using an effective concentration of 6.5 mg mL-1 DMP ...
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bioRxiv - Immunology 2023Quote: ... tumor cell suspensions were incubated 10 min at RT with Fc block (Leico; C381) and a viability dye (Biolegend; Zombie Aqua (ref # 423102) or Zombie NIR (ref # 423106) ...
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bioRxiv - Cancer Biology 2024Quote: ... OS cells were seeded into 24-well plates and incubated for 72 hours in basal medium alone or basal media supplemented with 10 ng/ml of either recombinant IL1α or IL1β (Biolegend, 570004 and 579404). Following incubation ...
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bioRxiv - Immunology 2024Quote: ... and activated for 72h with 10 µg/mL of plate-bound Ultra-LEAF™ purified anti-human CD3 monoclonal antibody (clone OKT3, BioLegend, cat. 317325) and 5 µg/mL of soluble Ultra-LEAF™ purified anti-human CD28 monoclonal antibody (clone 28.2 ...
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bioRxiv - Immunology 2022Quote: ... table 1) were diluted in blocking solution (F4/80 [Bio-Rad]: 10 µg/ml, Ly6G [abcam]: 1 µg/ml, MHC-II [BioLegend]: 5 µg/ml), CD163 [Invitrogen] ...