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Citations for BioLegend :
751 - 800 of 863 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Tregs were split and incubated at a concentration of 1 x 106 cells/mL cRPMI for 30 minutes at 37°C in cRPMI with 10 µg/mL anti-CD226 mAb (BioLegend) or IgG2a isotype control (BioLegend) ...
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bioRxiv - Immunology 2024Quote: ... Cells were cultured at a density of 0.5x10^6 cells/ml in complete RPMI medium and stimulated with plate-bound αCD3 (10 μg/ml, clone 145-2C11, BioLegend) and soluble αCD28 (2 μg/ml ...
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bioRxiv - Immunology 2024Quote: Naïve CD4+ T cells isolated from WT mice spleen and LN were treated with or without 10 µg/ml anti-β2 integrin non-function blocking antibody (BioLegend) for 1 hour and added onto 96 well plate coated with anti-CD3/CD28 along with either 2.5µg/ml of rLum or rBgn or rDcn or 1µg/ml of ICAM-1 for 30 minutes to check phospho-Akt-1 expression or 6 hours to check activation CD69 expression or 72 hours to check proliferation (CD4+ T cells were pre-labeled with CFSE for proliferation assay) ...
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bioRxiv - Immunology 2024Quote: ... and/or a combination of murine recombinant mIL-12 (10 ng/mL) and mIL-18 (50 ng/mL) together with Monensin (1X) and Brefeldin A (1X) (BioLegend) to detect cytotoxicity and IFNγ production ...
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bioRxiv - Immunology 2024Quote: ... Fc Receptors were blocked for 10 min before staining with fluor-conjugated antibodies (for FACS) and TotalSeq-A antibodies (Biolegend) in D-PBS 2% FBS for 25 min on ice ...
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bioRxiv - Immunology 2024Quote: Data was analyzed using FlowJo software (Version 10; FlowJo LLC, Ashland, OR, USA) and cytokine data was analyzed using LEGENDplex Data Analysis Software (BioLegend).
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bioRxiv - Immunology 2024Quote: ... cells were treated with 10 µg/ml of Ultra-LEAFTM purified anti-mouse IFNγ antibody (clone R4-6A2, Biolegend, #505709), 10 µg/ml of Ultra-LEAFTM purified anti-mouse TNF antibody (clone MP6XT22 ...
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bioRxiv - Microbiology 2024Quote: ... the NPCs were blocked by 1% anti-mouse CD16/CD32 for 10 min and then stained with AF647 anti-F4/80 (1:200, BioLegend) for 20 min ...
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bioRxiv - Pathology 2024Quote: PFA-fixed tissue sections were blocked with normal donkey serum (10% vol/vol) and incubated at 4 °C overnight with primary antibodies anti-F4/80 (123102, BioLegend), anti-Col1a1 (NB600-408 ...
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bioRxiv - Bioengineering 2024Quote: Proapoptotic protein and cytokine release was measured in cell-free supernatants from 16h co-cultures of CAR-T and tumor cell lines at 1:1 E:T ratio and from 48h co-cultures of CAR-T and PDO at 10:1 E:T ratio using the Legendplex™ multi-plex bead-based immunoassay (cat. # 741187, Biolegend). For cytokine measurement ...
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bioRxiv - Genomics 2024Quote: ... Cells were then cultured for 12-18h in DMEM/10%FBS supplemented with 100ng/mL mSCF/mIL-3/mIL-6 (BioLegend) at 37°C at 5% CO2 [Step Four] ...
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bioRxiv - Cancer Biology 2024Quote: ... Mouse IL-10 levels were measured using a commercial ELISA quantification kit according to the manufacturer’s instructions (BioLegend, Cat# 431411).
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bioRxiv - Systems Biology 2024Quote: ... after which the supernatant was discarded and 100µL of MojoSortTM Streptavidin Nanobeads (1:10 diluted in FACS buffer, Biolegend, 480016) was added to the mixture ...
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bioRxiv - Immunology 2021Quote: ... cells were split into 2 new 96-well plates and incubated with freshly prepared supplemented RPMI media with IL-2 (10 ng/ml, BioLegend, #575402). Cells were cultured for a total of 5 days before injection ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were pelleted by centrifugation and then resuspended in 100 μL FACs buffer (PBS with 10 % FCS) with PE-conjugated anti-CD61 (RRID:AB_313084, Biolegend, 104307, 1:200) and Alexa Fluor 647-conjugated anti-SSEA1 (RRID:AB_1210551 ...
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bioRxiv - Immunology 2021Quote: Mouse B cells were bulk sorted from mixed chimeras into Eppendorf tubes containing cold sterile PBS 10% FCS and incubated for 20 min at 4°C with TotalSeq™ DNA-barcoded anti-mouse ‘Hashing’ antibodies (BioLegend) at a 1/100 final dilution ...
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bioRxiv - Microbiology 2021Quote: ... and resuspended in RPMI with 10% FBS medium and were stimulated for 6 h with a cell activation cocktail containing PMA and ionomycin (Biolegend, # 423302) in the presence of protein transport inhibitor ...
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bioRxiv - Biophysics 2021Quote: ... The chambers were then incubated with 10 μg/ml purified Anti-HA 11 Epitope tag antibody (BioLegend, San Diego, CA, USA) in BIB at room temperature for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... mouse monoclonal anti-DnaK 4RA2 at 1□:□10,□000 dilution (Enzo, ADI-SPA-880) and HRP Goat anti-mouse IgG (BioLegend, 405306) at 1□:□10,□000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... WT and CD56 KO NK92 and YTS cells were incubated for 20 mins at 37°C and 5% CO2 on .5 glass coverslips that had been pre-coated with 10 μg/ml anti-CD18 (clone IB4) and anti-NKp30 (clone P30-15, Biolegend, NK92) or anti-CD28 (clone CD28.2 ...
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bioRxiv - Immunology 2021Quote: ... permeabilized with saponin 0.2% for 20 min and stained with PE-Cy7-anti-IFN-γ mAb and/or PE-anti-IL-10 mAbs (Biolegend). For TF and GzB/PRF staining ...
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bioRxiv - Microbiology 2021Quote: ... followed by washing and incubated with a solution of AlexaFluor® 647 conjugated anti-His antibody (BioLegend #652513, 10 µg/mL). After washing and drying ...
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bioRxiv - Cancer Biology 2020Quote: ... were treated with 10 Gy (as described above) and either PMX205 or vehicle and either IL-10 blocking antibody (LEAF Purified clone JES5-16E3, Biolegend #505012) (50 μg 24 hours before irradiation ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL solution were then stained with 3 μL FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μL Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... were added to the tumor cells and incubated for 2 hours with CD20-TCB (200 ng/ml) and +/- αLFA1 antibody (Clone: TS 1/18, 10 μg/ml, BioLegend, 302116). Cells were then gently washed ...
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bioRxiv - Microbiology 2021Quote: ... were injected intravenously into the tail vein with 10-15 μg of Alexa Fluor™ 647-coupled anti-CD31 antibody (clone 390, Biolegend) to label blood vessels ...
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bioRxiv - Microbiology 2020Quote: ... Splenic cell suspensions were treated additionally with red blood cell lysis buffer at room temperature for 10 min (Sigma-Aldrichor BioLegend Inc) for removing RBCs to obtain single cell suspensions ...
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bioRxiv - Cell Biology 2021Quote: ... the cell pellets washed twice with 10% FCS in PBS (100μl/well) and then resuspended in 10% FCS in PBS (100μl/well) containing APC/Cyanine7 Goat anti-mouse IgG (Biolegend; 1:250) and incubated on ice for 30mins ...
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bioRxiv - Developmental Biology 2020Quote: Cells obtained from embryos and stained with Epha1 antibodies as described above were then washed twice with PBS/10% DS and processed for staining using True-NuclearTM transcription factor Buffer Set (BioLegend #424401) according to the manufacturer’s instructions and following the protocol described in (Aires et al. ...
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bioRxiv - Immunology 2020Quote: ... TnT cells and Jurkat-derived cell lines were stimulated overnight with either 10 μg ml−1 plate-bound anti-human CD3e antibody (clone OKT3, BioLegend, #317326) or 1X eBioscience Cell Stimulation Cocktail (81 nM PMA ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained for 10 minutes at room temperature with Ghost Dye Violet 510 and Fc receptor blocking solution (Human TruStain FcX™, BioLegend) and washed once in FACS buffer ...
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bioRxiv - Immunology 2021Quote: ... Monocyte isolation was determined with the following flow cytometry antibody panel after 10 min incubation with anti-human CD32 (StemCell – 18520): APC anti-human CD14 (Biolegend – 301808), PE-Cy7 anti-human CD3 (Biolegend – 344186) ...
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bioRxiv - Immunology 2021Quote: ... were co-cultured with 1×105 relevant target cells (10:1 E:T ratio) overnight in the presence of Brefeldin A (BFA) (Biolegend, CA, USA), and the next day ...
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bioRxiv - Immunology 2021Quote: ... The following flow cytometry antibody panel was used to determine monocyte differentiation and M1 polarization after 10 min incubation with anti-human CD32: APC anti-human CD14 (Biolegend – 344186) BV421 anti-human CD80 (Biolegend – 305222) ...
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bioRxiv - Immunology 2021Quote: Leukocytes from whole blood and ETA samples were incubated with oligo-conjugated Ig-A/D/G/M for 10 min at 4°C followed by the addition of Human TruStain FcX™ (BioLegend) and 10 min incubation at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... Collected cell populations were immediately seeded in tissue culture at a concentration of 2x105 cells per 1.9 cm2 in DMEM containing 5% FBS and 10 ng/mL TGFβ (Biolegend Cat 763102) or 500 nM Prostaglandin F (Cayman Chemical Cat 16010).
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bioRxiv - Cancer Biology 2023Quote: ... T cells transfected with pMAX-GFP and either pcDNA3.1-Rai or empty vector were added in a 10:1 ratio and incubated for 4 h in presence of APC anti-LAMP1 antibody (clone H4A3, Biolegend #328620). Monensin (2mM ...
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bioRxiv - Neuroscience 2023Quote: ... leptomeninges and brain cells were pelleted at 300xg for 10 minutes and incubated with anti-CD11b APC/Cy7 (BioLegend cat. #101226) and anti-CD45 BV510 (BD Biosciences cat ...
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bioRxiv - Immunology 2023Quote: ... The sample pool was washed once with 10 mL of PBS-BSA and resuspended in 150 µL of Zombie NIR viability dye (1:500 in PBS; BioLegend 423105). After 15 minutes on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... for 10 min at 4°C followed by an incubation with CD45-BV421 (Biolegend 103133, clone 30-F11, 1:200 dilution) and Ter119-BV421 (Biolegend 116233 ...
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bioRxiv - Cancer Biology 2022Quote: Oligo-labeled antibody staining was done on both melanoblasts and melanom cells using the TotalSeq™-A Antibodies and Cell Hashing with 10× Single Cell 3′ Reagent Kit (Biolegend). The HashTag Oligonucleotidess used for the analysis are mHTO01 ...
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bioRxiv - Cancer Biology 2022Quote: ... spun at 700 g for 3 minutes at 4°C and washed in MACS Buffer 3× before being incubated with the following antibodies: APC-conjugated rat anti-CD3 (5 μL/10×106 cells; Biolegend, 100408), PE-conjugated rat anti-CD8b (2.5 μL/10×106 cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Splenocytes from naive or tumor-bearing AST;Cre-ERT2 mice were mixed with 2×106 C57BL/6 splenocytes and incubated in RPMI-10 for 4 hours at 37°C in the presence of brefeldin A (Biolegend 420601) and TAG peptide (SAINNYAQKL [0.5 μM] ...
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bioRxiv - Immunology 2024Quote: ... that were pulsed with epitope (10 ng/mL) was collected and IFN-γ production was measured with an enzyme-linked immunosorbent assay (ELISA, BioLegend) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Murine DCs were differentiated from isolated bone marrow via culture in RPMI + 10% FCS 1% Pen/Strep and 1% Amino Acids containing 20 ng/ml GMCSF (Biolegend, #576306) on silicone hydrogels with different stiffnesses ...
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CD56/NCAM mediates cell migration of human NK cells by promoting integrin-mediated adhesion turnoverbioRxiv - Cell Biology 2023Quote: ... at 37°C and 5% CO2 on #1.5 glass coverslips that had been pre-coated with 10 µg/ml anti-CD18 (clone IB4) and anti-NKp30 (clone P30-15, Biolegend; 325202) or 5 µg/ml Fc-ICAM-1 (Enzo Life Science ...
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bioRxiv - Physiology 2023Quote: ... Cisplatin is washed off and non-specific binding was blocked with a 10-minute staining of cells with Fc Block (Biolegend; 1µg). Cells were stained with 0.5µg of cell surface metal-conjugated antibodies for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... BMDMs were exposed to their indicated treatment condition in complete RPMI overnight (∼18 hours): IFNγ stimulation (10 ng/mL; BioLegend, 575304); supplementation with pyruvate or nicotinamide (NAM ...
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bioRxiv - Genomics 2022Quote: ... 0.2×106 naïve CD4+ T cells were cultured in presence of 10 ng/mL of recombinant IL-12 (BioLegend, Cat#577002), 1 ng/mL of recombinant IL-2 (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... LEPs from co-cultures were separated after 10 days for gene expression qPCR analysis by FACS using anti-human CD133-PE (BioLegend, clone7) and anti-human CD271-APC (BioLegend ...