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Citations for BioLegend :
751 - 800 of 7620 citations for 14 Nonylphenoxy 3 6 9 12 tetraoxatetradecan 1 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... streptavidin-conjugated MBs were washed once by centrifugation and incubated with 6 µg biotin anti-human αvβ3 antibody (304412, BioLegend) on ice for 30 min ...
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bioRxiv - Immunology 2024Quote: ... Splenocytes and ascitic cells were isolated and re-stimulated with SIINFEKL peptide (immunodominant ovalbumin peptide) for 12 hours in the presence of 2 uM Monensin (BioLegend, San Diego, CA) with the following intracellular staining of IFN-γ and measurement of IFN-γ-producing CD8+ T cells (meaning ovalbumin-specific T cells ...
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bioRxiv - Cancer Biology 2024Quote: ... CD90-2-APC (17-0902-82, eBioscience™), CD44-PE (12-0441-82, eBioscience™), CD45-APC (17-0454-82, eBioscience™) and CD19-APC-Cy7 (115501, Biolegend). Mouse isotype-matched IgG served as a negative control ...
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bioRxiv - Cancer Biology 2021Quote: NK cells were isolated from C57BL/6 mice (male and female) with the MojoSort mouse NK cell isolation kit (BioLegend) (purity >85 % based on the flow cytometric analysis with NK1.1 and CD49b antibodies) ...
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bioRxiv - Cancer Biology 2021Quote: ... The following anti-mouse antibodies we used for surface staining: Alexa Fluor 647-labeled H-2Kb/H-2Db – MHC Class I (clone 28-8-6, Biolegend); PE-labeled I-A/I-E – MHC Class II (Clone M5/114.15.2 ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL were then stained with 2.5 μL FITC anti-human CD19 antibody (BioLegend, 392508), 2.5 μL FITC anti-human CD20 antibody (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Phenotypes of B cells before and after 6 days of culture were determined by flow cytometric analysis using mouse monoclonal antibody clones: CD19 PECy7 HIB19 (Biolegend), CD27 BV421 O323 (Biolegend) ...
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bioRxiv - Genomics 2020Quote: ... 1.25 × 10^6 PBMCs were spun down and resuspended in 25 μl CITEseq antibody cocktail (TotalSeq™-A Antibodies, BioLegend) containing among others one hashing antibody per each sample ...
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bioRxiv - Microbiology 2021Quote: ... prior to staining cells in 100 µL perm/wash with 0.5 µL APC anti-human IL-6 antibody (Cat# 501112, Biolegend®) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... The sections were then rinsed in a washing solution twice before nuclei were stained with 4’,6-diamidino-2-phenylidole (DAPI; BioLegend). The sections were rinsed with washing solution one final time and then mounted on slides for examination under an Olympus FV1000 laser-scanning 4-channel confocal microscope (Olympus).
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bioRxiv - Immunology 2024Quote: ... Flow cytometry analysis of CTLA4-mediated trans-endocytosis was performed as previously described [6] using the True-NuclearTM Transcription Factor Buffer Set (BioLegend) and the following intracellular antibody panel ...
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bioRxiv - Immunology 2023Quote: ... with recombinant IL-15/IL-15RA complex (100 ng/ml; Thermo Fischer, Waltham, MA) or recombinant mouse IL-6 (50 ng/ml, BioLegend), TGF- β1 (1ng/mL) ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were then tagged with mouse Total-Seq anti-mouse hashtags C0306 (Brafi: Hashtag 6) and C0307 (Brafi + C-Me: Hashtag 7) (BioLegend) according to manufacturers’ protocols.
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bioRxiv - Developmental Biology 2023Quote: ... NPCs were we pelleted and washed once with AutoMACS buffer before resuspending AutoMACS with in DAPI (dead cell dye DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate, 422801, BioLegend) and DRAQ5 (DRAQ™ Live cell dye ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... CD3+ T cells were purified from C57BL/6 spleen cells with MojoSort mouse CD3 T cell isolation kit (Biolegend, USA), while CD8+T cells were isolated by adding biotin-labeled anti-CD4 antibody into MojoSort mouse CD3 T cell isolation kit ...
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bioRxiv - Physiology 2023Quote: ... IL-6 and IL-10 in the homogenates were measured using LEGENDplexTM Mouse Custom Panel (BioLegend, San Diego, CA, USA). Assay was performed in the Attune NxT flow cytometer (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... from uninfected or MCMV-infected WT C57BL/6 mice were sorted as described and stained with barcoded antibodies (Total-Seq B, BioLegend). After hash-staining ...
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bioRxiv - Immunology 2024Quote: ... derived from OT-1 mice treated with either CATR or vehicle were restimulated with 1µg/ml OVA-peptide for 6 hours and Brefeldin A (Biolegend, 420601) for 4hr prior to analysis.
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bioRxiv - Molecular Biology 2024Quote: IL-6 KO mice with cast immobilization for 24 h were subjected to intraperitoneal administration of 400 ng of rIL-6 (575702, BioLegend) in saline or of saline alone ...
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bioRxiv - Immunology 2024Quote: ... Cells were cultured at a density of 0.5x10^6 cells/ml in complete RPMI medium and stimulated with plate-bound αCD3 (10 μg/ml, clone 145-2C11, BioLegend) and soluble αCD28 (2 μg/ml ...
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bioRxiv - Immunology 2024Quote: ... naïve CD4 and CD8 T cells were isolated from the spleen and peripheral LNs of C57BL/6 mice using the MojoSort™ Mouse CD4 or CD8 Naïve T Cell Isolation Kit from BioLegend and irradiated with 254 nm-UV light (600 mJ/cm2 using a Stratalinker 2400) ...
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bioRxiv - Immunology 2024Quote: ... Fluorescently labeled anti-mouse antibodies against CD45 (104) and H-2Kb/H-2Db (28-8-6) were obtained from BioLegend.
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bioRxiv - Microbiology 2024Quote: ... BMDMs were isolated from the femurs of 6-week-old Balb/c mice (Taconic Laboratories) and differentiated by growth in (BioLegend) in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Immunology 2024Quote: ... All other cytokines were assessed using Mouse M1 Macrophage LEGENDplex flow-based assay (CXCL1, IL-1β, IL-6, IL-12p40, IL-12p70, IL-18, IL-23, TNFα; BioLegend) with BD LSRII flow cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... were independently stained with TotalSeq™-B0303 anti-mouse Hashtag 3 Antibody (Biolegend, cat: 155835) according to the manufacturer’s instructions and with fluorophore-conjugated antibodies for cell sorting (anti-CD45.1-PB ...
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bioRxiv - Immunology 2021Quote: ... 3) mouse anti-human CD196 prelabeled with Alexa Fluor 488 (CCR6 marker; clone G034E3, BioLegend) and 4 ...
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bioRxiv - Immunology 2021Quote: ... αCD366 (Tim-3) BV711(RMT3-23) and αTIGIT PE-Dazzle594 (2G9) were purchased from BioLegend, San Diego ...
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bioRxiv - Immunology 2021Quote: Macrophages (~3 x 106) were stained with anti-F4/80 (rat monoclonal FITC-conjugated, BioLegend) in a 1:200 dilution in FACS buffer for 20 minutes on ice ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated with Brilliant Violet 421 anti-CD56 (clone HCD56, Biolegend, 3 μg/ml), APC anti-NKp30 (clone P30-15 ...
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bioRxiv - Cancer Biology 2020Quote: Samples were stained with 10X Chromium 3’ mRNA capture compatible TotalSeq-A antibodies (Biolegend, USA). Staining was performed as previously described by Stoeckius et ...
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bioRxiv - Microbiology 2023Quote: ... lysed in 1X red blood cell lysis buffer for 3 minutes (ref no. 420301, BioLegend), and passed successively through 70μm and 30μm strainers (Miltenyi Biotec) ...
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bioRxiv - Pathology 2023Quote: ... and resuspended in 3 ml red blood cell lysis buffer (Biolegend, San Diego, CA, USA) for 90 s to remove the remaining red blood cells and diluted in 9 mL PBS after incubation ...
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bioRxiv - Pathology 2023Quote: ... and suspended in 3 ml red blood cell lysis buffer (Biolegend, San Diego, CA, USA) for 90 s to remove the remaining red blood cells and diluted in 9 mL PBS after incubation ...
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bioRxiv - Immunology 2024Quote: ... and 3 µg of Ultra-LEAFTM purified anti-mouse CD28 antibody (clone 37.51, Biolegend, # 102116) per well ...
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bioRxiv - Immunology 2021Quote: ... Cells harvested during the conversion period from CD34+ hematopoietic precursors to CD14+ monocytes (Days 12-21 of the differentiation protocol) were stained with αCD14-PE (BioLegend, San Diego, CA, USA) and αCD34-APC (BioLegend) ...
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bioRxiv - Microbiology 2021Quote: ... and resuspended in RPMI with 10% FBS medium and were stimulated for 6 h with a cell activation cocktail containing PMA and ionomycin (Biolegend, # 423302) in the presence of protein transport inhibitor ...
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bioRxiv - Developmental Biology 2021Quote: ... Surface staining was done by adding the flow cytometry antibody cocktail outlined in Extended Data Table 6 containing F4/80 (BioLegend, 123120), CD11b (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were induced to differentiate by culturing in the absence of β-Estradiol for 6 days before cells were stained for CD11b (Biolegend) and analyzed on the LSRII flow cytometer (BD Biosciences) ...
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bioRxiv - Cancer Biology 2024Quote: ... Splenocytes from naive or tumor-bearing AST;Cre-ERT2 mice were mixed with 2×106 C57BL/6 splenocytes and incubated in RPMI-10 for 4 hours at 37°C in the presence of brefeldin A (Biolegend 420601) and TAG peptide (SAINNYAQKL [0.5 μM] ...
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bioRxiv - Microbiology 2023Quote: ... and subsequently with biotinylated α- CADM1 antibody (MBL, Cat. No. CM004-6) and streptavidin-conjugated secondary antibody (BioLegend, Cat. No. 405226). Cells were resuspended in 4◦C PBS 2% FCS before cell sorting.
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bioRxiv - Cancer Biology 2023Quote: Plasma Ig were analysed using the LEGENDplex Mouse Immunoglobulin Isotyping panel (6-plex) by flow cytometry (Cat #740493, BioLegend, CA, USA). Plasma sample and standards was prepared using assay buffer as per the manufacturer’s instruction ...
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bioRxiv - Physiology 2023Quote: The levels of TNF-α and IL-6 in the serum and the supernatant of the crushed muscle tissues were measured using a commercial kit (mouse TNF-α and IL-6 ELISA max standard set, BioLegend). The values of TA and GA muscles were revised to the protein concentration measured by BCA kit and expressed as pg/mg.
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bioRxiv - Immunology 2023Quote: ... CD3+ T cells were isolated from spleens of 6-to-8-week-old OT-II mice by MojoSort mouse CD3+ T cell isolation kit (Biolegend 480024). Stimulated FLS were washed with PBS and co-cultured with CD3+ T cells at a concentration of 5-10×105 cells/mL in 10% RPMI for 5 days with either the cognate OVA323-339 antigen (2μg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... Serum IL-6 and TNF-α concentrations were determined with enzyme-linked immunosorbent assay (ELISA) kits (Legend Max Mouse IL-6 ELISA Kit, BioLegend, and Mouse TNF Alpha Uncoated ELISA ...
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bioRxiv - Immunology 2024Quote: ... Lysis reaction was stopped by adding 10ml of PBS/2mM EDTA + 2% FBS and the resuspended solution was centrifuged 5min at 350g.Spleen was taken from 8- to 16-week-old mice and pressed in a 6 wells plate containing 2ml of red blood lysis buffer (Biolegend #420302) 2 more ml were added and then incubated 5min at RT ...
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bioRxiv - Cancer Biology 2024Quote: Spleen was isolated from C57BL/6 mice and CD8 T cells were isolated using MojoSort™ Mouse CD8 T Cell Isolation Kit (Biolegend). The cells were stimulated with Dynabeads™ Mouse T-Activator CD3/CD8 kit (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... BMDCs were harvested 3 days later and stained with antibodies specific for CD11c (clone N418, BioLegend) and MHC-I (H-2Kb ...
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bioRxiv - Immunology 2021Quote: ... mice received IV injection of 3 μg of a Brilliant Violet 570-conjugated CD45 antibody (BioLegend) and sacrificed 3 min later and the fraction of IV-labeled cells was quantified.
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bioRxiv - Genetics 2020Quote: ... Cells were stimulated for 3 hours with 2.5 μg/ml of anti-CD3 (Biolegend, catalog 317315) and anti-CD28 (Biolegend ...
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bioRxiv - Immunology 2022Quote: Cytokine concentration in mouse plasma samples were quantified by a 3-plex LEGEND-plex assay (Biolegend) that contained capture beads and detection antibodies for CCL2 (monocyte chemoattractant protein-1 ...