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Citations for BioLegend :
701 - 750 of 7620 citations for 14 Nonylphenoxy 3 6 9 12 tetraoxatetradecan 1 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Sections were then blocked in blocking buffer (3% normal serum block (Biolegend, 927503), and 0.4% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... and SCT Dd or SCT Dd-CD4 cell surface expression was regularly assessed by flow cytometry (clone 34-5-85 and 34-2-12, Abcam and Biolegend, respectively). Transfectants were FACS-sorted (FACSDiva and FACSARIAIIIU cell sorter ...
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bioRxiv - Immunology 2020Quote: The serum levels of a total of 12 virus-related cytokines were measured by a bead-based immunoassay panel (Mouse Anti-Virus Panel, Cat No: 740622, Biolegend, USA). The BALF levels of a total of 12 inflammatory cytokines were measured by a bead-based immunoassay panel (Mouse Inflammation Panel ...
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bioRxiv - Immunology 2020Quote: ... The BALF levels of a total of 12 inflammatory cytokines were measured by a bead-based immunoassay panel (Mouse Inflammation Panel, Cat No: 740446, Biolegend, USA) on CytoFLEX Flow Cytometer (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: Splenocytes from intramuscularly vaccinated mice were incubated in culture with a pool of 253 overlapping 15-mer SARS-CoV-2 S peptides (Grifoni et al., 2020) for 12 h at 37°C before a 4 h treatment with brefeldin A (BioLegend, 420601). Following blocking with FcγR antibody (BioLegend ...
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bioRxiv - Microbiology 2023Quote: Splenocytes from intramuscularly vaccinated mice were incubated in culture with a pool of 253 overlapping 15-mer SARS-CoV-2 S peptides(25) for 12 h at 37°C before a 4 h treatment with brefeldin A (BioLegend, 420601). Following blocking with an anti-FcψR antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Ce3D™ Wash Buffer was removed completely and samples were placed in Ce3D™ Tissue Clearing Solution (12 hr, room temperature, with gentle shaking; 427704, Biolegend). Images were taken with Leica SP8 Confocal System (Leica Microsystems).
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bioRxiv - Genomics 2022Quote: ... 0.2×106 naïve CD4+ T cells were cultured in presence of 10 ng/mL of recombinant IL-12 (BioLegend, Cat#577002), 1 ng/mL of recombinant IL-2 (BioLegend ...
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bioRxiv - Systems Biology 2021Quote: ... The levels of interleukin (IL)-6 and tumor necrosis factor (TNF)-α were detected using ELISA kits (Biolegend) following the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: ... HTO staining was carried out with 0.5μg of β2M and CD298 antibody-conjugated oligonucleotides (Total-seq B Hastags 4, 6 and 8; BioLegend) with ∼500,000-750,000 isolated single cells ...
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bioRxiv - Immunology 2020Quote: ... mouse TNF-α (430904) and IL-6 (431304) enzyme-linked immunosorbent assay (ELISA) kits were purchased from BioLegend; Griess Reagent System (G2930 ...
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bioRxiv - Bioengineering 2021Quote: ... FITC anti-mouse H-2Kb/H-2Db Antibody (Clone 26-8-6) were from Biolegend (San Diego, CA). Primary antibodies of STING/TM173 (D2P2F) ...
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bioRxiv - Immunology 2022Quote: ... and two hashtag antibodies were built by combining the antibodies MHC I-Biotin (Biolegend, clone 28-8-6) and CD45-Biotin (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... CD45-labelled cells were mixed (maximum 6 samples together) and stained with APC-conjugated FCRL5 antibodies (Biolegend #340305)(for panel #2) ...
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bioRxiv - Immunology 2024Quote: ... and IL-6 were measured by LEGENDplex flow-based 13-plex mouse inflammation panel kit from Biolegend (740446) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Cell supernatant was collected after 24 h to measure IL-6 and TNF-α levels using ELISA (BioLegend) according to manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Release of IL-6 and IL-8 were measured using commercial ELISA kits (Biolegend, San Diego, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... injections of a TH17 priming cocktail (n=8; 0.5μg rmIL-6 [575704, BioLegend, USA] and 1μg rmTGF-β1 [763104, BioLegend, USA]) or rest (n=8 ...
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bioRxiv - Microbiology 2024Quote: ... IL-6 and TNF-α ELISA kits at proper dilutions according to the manufacturer’s instructions (BioLegend, CA, USA). For the detection of cytokines in serum and peritoneal fluid ...
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bioRxiv - Immunology 2024Quote: ... the Siglec+ population was sorted by staining the cells with anti-Siglec-7 APC (BioLegend, clone 6-434) or anti-Siglec-9 APC (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... the individual antibody hit at 5 μg/ml and/or ACE2-mFc was incubated with 1-2 million 1D8 spike+ cells and detected by anti-human-Fc PE (invitrogen, 12-4998-82) and FITC Goat anti-mouse-IgG (Biolegend, cat#405305) or APC Goat anti-mouse-IgG (Biolegend ...
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bioRxiv - Bioengineering 2024Quote: ... Culture supernatants were collected and processed for cytokine analysis using a mouse Th cytokine panel (12-plex) cytometric bead assay (BioLegend, Amsterdam, Netherlands). Flow cytometry was performed on a MACSQuant II Flow cytometry instrument (Miltenyi) ...
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bioRxiv - Cell Biology 2024Quote: ... were coated overnight at 4 °C with 100 μl Tau-12 capture antibody per well (2 μg/ml; Biolegend, cat. no 806502) in 20 mM NaH2PO4 pH 7.4 ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were analyzed using the Legendplex mouse T helper cytokine panel version 3 (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were suspended in 3 mL of RBC lysis buffer (Biolegend, CA, USA), allowed to stand at room temperature for 3 minutes ...
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bioRxiv - Immunology 2023Quote: ... B cells were stimulated in vitro with anti-CD40 antibody (clone HM40-3, Biolegend) overnight to fully activate HEL+ B cells ...
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bioRxiv - Cell Biology 2022Quote: ... or alternatively with Brilliant Violet 421 anti-mouse/human Galectin-3 antibody (125416, Biolegend) for 30 min at RT ...
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bioRxiv - Immunology 2024Quote: ... with 3 µg of APC-labeled anti-CD8b.2 antibody (clone 53-5.8, Biolegend) 5 min prior to euthanasia to mark vasculature-associated cells in vivo.
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bioRxiv - Neuroscience 2023Quote: ... The sections were washed 3 times with PBS and counter stained with DRAQ5 (BioLegend) to stain DNA for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... and Purified anti-Tubulin β 3 (TUBB3) Antibody (#801201, RRID:AB_2313773; BioLegend, San Diego, CA).
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bioRxiv - Immunology 2022Quote: A 3-plex SARS-CoV-2 Serological IgG Panel (BioLegend LEGENDplex, Cat. No. #741132) was used for measuring IgG titre of the 3 key COVID-19 coronavirus derived proteins ...
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bioRxiv - Biochemistry 2024Quote: ... and 3 μg/mL of conjugated H3 pSer10 antibody (Alexa Fluor® 594, BioLegend), which served as a marker for M phase ...
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bioRxiv - Cancer Biology 2024Quote: MUTZ-3 and UCSD-AML1 cells were stained with anti-CD34-APC (BioLegend, 343607). MUTZ-3 cells were also stained with anti-CD14-PE-Cy7 (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... were co-incubated with 6×105 target cells expressing mCherry at 37°C for 2 hours in the presence of 2µM monensin (BioLegend). The cells were centrifuged ...
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bioRxiv - Microbiology 2020Quote: ... cells were counted and plated on 6 well plates in RPMI + 5% FBS + 20 ng/ml GM-CSF (BioLegend). Cells were maintained at 37° C 5% CO2 and supplemented on day 3 ...
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bioRxiv - Genetics 2022Quote: ... two-week C57BL/6 c-Kit+ bone marrow cells were cultured in media with recombinant mouse interferon alpha (Biolegend) as indicated with media changes every 2-3 days ...
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer’s instructions and stained with PE anti-IL-6 and PE-Cy7 anti-IL-10 (JES3-9D7) Abs (Biolegend).
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bioRxiv - Immunology 2021Quote: T cells were enriched from single cell suspensions of splenocytes isolated from naïve C57BL/6 mice as for flow cytometry using a mouse CD3 T cell negative selection kit (Biolegend). Enriched CD3+ T cells were cell trace stained and cultured at 1x106 cells/mL and stimulated with mouse T-activator anti-CD3/CD28 Dynabeads (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: Human and mouse IL-6 and TNF-α protein were measured by an enzyme-linked immunosorbent assay (ELISA) (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Human Th17 differentiation assays were performed similarly except the concentrations of recombinant human IL-6 (30 ng/ml, BioLegend), TGF- β1 (2.25ng/mL) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then fixed in 3.7% formaldehyde for 6 min and PM HA-GLUT4-GFP detected by an anti-HA antibody (901503, BioLegend). HA staining was visualized by Cy3-goat anti-mouse secondary antibody (115-165-062 ...
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bioRxiv - Immunology 2023Quote: Appropriate ELISA kits were used to quantify human or murine IL-6 and/or TNF concentrations according to manufacturer’s recommendations (Biolegend). The 450 nm absorbance was measured by a SpectraMax® plate reader.
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bioRxiv - Immunology 2022Quote: ... male mice were isolated 6 dpi with NP-OVA/alum and stained with anti-CD4 Alexa Fluor 647 (GK1.5, BioLegend), anti-TCRβ PE-Cy7 (H57-597 ...
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bioRxiv - Biochemistry 2024Quote: ... the BMMs were seeded into 6-well plates and incubated with macrophage colony-stimulating factor (M-CSF) (20_ng/ml, Biolegend) and Receptor activator of nuclear factor kappa-Β ligand (RANKL ...
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bioRxiv - Immunology 2023Quote: ... both endometriosis and sham mice were treated with intraperitoneal (i.p.) injections of 1μg of rmIL-23 (n=6/group; 589006, BioLegend, USA), or PBS for controls (n=6/group) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cat #126404), and PE anti-mouse H2-Kb/H2Db antibody (28-8-6, cat#114607) were purchased from BioLegend. Anti-VHL (VHL40 ...
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bioRxiv - Immunology 2022Quote: ... For intracellular staining cells were either permeabilized with 0.5% Saponin or 0.1% Triton and stained for TNF-α and/or IL-6 (BioLegend). Cells were washed with 2% serum supplemented 1X PBS and subsequently fixed with 1% PFA ...
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bioRxiv - Cancer Biology 2022Quote: Cells cultured in 2D were seeded at 5 × 105 cells/well in 6-well plates and incubated with recombinant human interferon gamma (IFNγ) (0.5 ng/mL) (Biolegend) and/or recombinant tumour necrosis factor alpha (TNFα ...
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bioRxiv - Immunology 2023Quote: IL-6 secretion was quantified using an ELISA MAX Deluxe Set Mouse IL-6 according to the manufacturer’s instructions (431304, Biolegend). Total and NP-specific antibody levels and IL-2 secretion were measured by ELISA on half-area 96-well plates (Greiner Bio-One ...
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Lipid Nanoparticles from L. meyenii Walp Mitigate Sepsis through Multimodal Protein Corona FormationbioRxiv - Bioengineering 2024Quote: ... Then the supernatant was collected to measure IL-6 and TNF-α levels using ELISA (Biolegend, San Diego, CA).