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Citations for BioLegend :
7751 - 7800 of 8294 citations for 1 6 ANHYDRO β D GLUCOSE 2 3 4 TRI O ACETATE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... cells were resuspended in 1mL of PBS and live/dead dye used at 1:500 (Zombie NIR, BioLegend #423106). Cells were washed with 5mL of FACS buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Two million viable cells were resuspended in 100µl FACS buffer with 1 µl of Fc Block (anti-mouse Fc block CD16/32, Cat# 101302, Biolegend) for 20 minutes at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... C57BL/6J mice bearing orthotopic KPC tumors were treated with 10 μg g−1 anti-TNF-α antibody (BioLegend) in combination with 100 mg/kg gemcitabine (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed with ice cold PBS and surface stain with primary anti-PD-L1 (1:50) antibody (BioLegend) on ice for 90 min ...
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bioRxiv - Cancer Biology 2023Quote: ... immunohistochemistry (IHC) and flow-cytometry (FC): Pacific Blue anti-mouse Ly-6A/E (Biolegend, Cat#108120, 1:50 FC), Sca-1/Ly6A/E (E13 161-7 ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were dissolved in 5% BSA (Biofroxx, 4240GR005) and the dilutions were: Streptavidin-Cy3 (1:200, BioLegend, 405215), RL2 (1:200 ...
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bioRxiv - Bioengineering 2023Quote: ... Successful Differentiation was confirmed by flow cytometry using CD41 (APC, clone HIP8, Biolegend Cat. No. 303710, Dilution 1:200) and CD42b (PE ...
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bioRxiv - Microbiology 2023Quote: ... T lymphocytes were evaluated using the following antibodies: rat anti-mouse IL-17A (TC11-18H10.1, 1/50; Biolegend, #506922), rat anti-mouse RORγt (AFKJS-9 ...
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bioRxiv - Genomics 2023Quote: ... Cells were stained on ice in the dark for 30 mins with CD34-APC (clone 581; Biolegend; 1:100), CD38-PE/Cy7 (clone HB7 ...
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bioRxiv - Immunology 2023Quote: ... were given by tail-vein intravenous (i.v.) injection in 200 μL of PBS with 1 μg/mL of anti-CD3 antibody (OKT3, Biolegend). Monthly post HPC transplant ...
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bioRxiv - Cancer Biology 2023Quote: ... FACS gates were set based on CD45 (Alexa Fluor® 594 anti-CD45, 1:20, Biolegend, #368520, clone 2D1), SYTOX Blue and DRAQ5 intensities to isolate live CD45+ and CD45-populations as shown in Supplementary Fig ...
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bioRxiv - Cell Biology 2024Quote: ... The sliced sections were incubated with an anti-HA monoclonal antibody (1:1000; BioLegend, San Diego, CA, USA, #901514), followed by anti-mouse Alex Fluor 488-conjugated immunoglobulin (1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were resuspended in cell staining buffer (PBS + 5 mM EDTA + 1% BSA + 1.6 mM NaOH + 0.01% sodium azide) and incubated in 1:100 diluted Zombie UV viability dye (Biolegend, 423107) or Zombie Violet viability dye (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: The Chemokine concentrations in cell culture media were assessed using the 13-plex LEGENDplex Human Inflammation Panel 1 (Biolegend), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Retinas were then placed in primary antibody solution containing 1:1000 mouse anti-SMI-32 (BioLegend, Catalog #: 801701, RRID:AB_509997) in 3% normal donkey serum in 0.3% Triton PBS for 2-4 days at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: MDA-MB-436 cells were resuspended in cell staining buffer (PBS + 5 mM EDTA + 1% BSA + 1.6 mM NaOH + 0.01% sodium azide) and incubated in 1:100 diluted Zombie Violet viability dye (Biolegend, 423113) for 5 minutes on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were first incubated with True Stain fcXTM antibody (anti-mouse CD16/32, BioLegend, #101319, clone 93, 1:100) in PBS containing 0.5% BSA and 2 mM EDTA before staining with antibodies directly conjugated to fluorophores for flow cytometry ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was blocked with 5% milk in Tris-buffered saline with 0.1% Tween (TBST) and incubated overnight with primary antibodies: mouse anti-STAT1 (1:800, Cat# 603702, Biolegend), mouse anti-STAT1 phospho (Ser727 ...
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bioRxiv - Immunology 2024Quote: ... 500ul of fresh cell culture media for each respective cell type were combined with 10 µl of 0.5ug/µl purified anti-human ICAM-1 blocking antibody (Clone HA58, BioLegend), added to the wells ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked in 5% BSA in Tris-buffered saline/0.05% Tween-20 and proteins were detected by specific primary antibodies against MBP (#SMI 99, 1:1000; Biolegend), MOBP (bs-11184R ...
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bioRxiv - Developmental Biology 2024Quote: ... and with biotinylated antibodies against a panel of lineage markers (CD19, CD11b, CD11c, NK.1.1, Ter119, CD3ε, Gr-1 and B220 (BioLegend)) ...
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bioRxiv - Cancer Biology 2024Quote: ... washed and re-suspended in the following primary antibodies: a rat monoclonal (IgG2a) anti-mouse FRβ (BioLegend,1:100) or a sheep polyclonal anti-mouse FRα (R&D Systems ...
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bioRxiv - Immunology 2024Quote: ... they were stained with 1 uL each of the following TotalSeqC surface antibodies: anti-human IgM (BioLegend, Cat#314547), anti-human IgD (BioLegend ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were then resuspended in 50uL of PBS containing fluorophore-conjugated antibodies for CD11b (1:100, Biolegend, 101216), MHCII (1:100 ...
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bioRxiv - Cell Biology 2024Quote: Antibodies used in this study were from the following sources: ALIX (634502, IF 1:100) was purchased from BioLegend. IST1 (51002-1-AP ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were washed with FACS buffer (PBS, 1% BSA, 0.1% NaN3) and incubated with Zombie Green viability dye (BioLegend) to exclude dead cells ...
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bioRxiv - Cell Biology 2024Quote: ... cells were labeled with antibodies detecting the following cell-surface markers: CD133-APC (Biolegend, clone 7, dilution 1:20), CD19-PECy7 (Biolegend ...
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bioRxiv - Synthetic Biology 2021Quote: ... The knock-out of the T cell receptor (TCR) was assessed with the absence of signal after staining 1:200 with APC-labeled anti-CD3ε (Biolegend). Each CAR construct contained a Strep tag which allowed for a two-step staining to validate successful knock-in ...
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bioRxiv - Cell Biology 2020Quote: ... Primary NK cells from lineage-depleted apheresis or NK92 cells were incubated on cells or cell-free matrix for 30 minutes in the presence of directly conjugated antibodies to surface antigens CD56 (1:50, clone HCD56, Biolegend) and CD29 (1:50 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell suspensions were incubated with the peptides for 1 hour following fixation with formaldehyde and staining with cytochrome c antibody (BioLegend, Alexa Fluor® 647 anti-Cytochrome c - 6H2.B4 ...
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bioRxiv - Immunology 2021Quote: ... The membrane was washed 3 times in TBST and incubated in TBST containing 5% non-fat dry milk and 1:1000 mouse anti-His (Biolegend) overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Unlabeled cells were then centrifuged at 300 x g and labeled with a cocktail of 1 μg/ml of PerCP-Cy5.5 anti-EPCAM antibody and Zombie Aqua viability stain (Biolegend 423101) diluted 1:400 from stock concentration in FACS buffer.
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bioRxiv - Cell Biology 2020Quote: ... followed by incubation on ice with labeling cocktail consisting of 1 μg/ml of PerCP-Cy5.5 anti-EPCAM antibody and Zombie Aqua viability stain (Biolegend 423101) diluted 1:400 from stock concentration in FACS buffer ...
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bioRxiv - Immunology 2021Quote: PBMCs were stained with the following antibodies and proteins for flow sorting: anti-CD19-APC (1:10 dilution, clone HIB19, cat. no. 982406, BioLegend), anti-IgD-FITC (1:20 dilution ...
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bioRxiv - Immunology 2021Quote: ... the cell suspensions were stained with the following antibodies: PE anti-mouse Ly-6C (BioLegend, 128008, clone HK1.4, 1:200), PerCP/Cyanine5.5 anti-mouse/human CD11b (BioLegend ...
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bioRxiv - Genetics 2021Quote: ... in 1 × phosphate-buffered saline with 0.1% Tween 20 (PBST) before being incubated with a 1:5000 dilution of anti-GAPDH-HRP (Biolegend, 649203) or 1:2000 dilution of anti-ACTIN-HRP (5125)
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bioRxiv - Microbiology 2021Quote: ... the cells were incubated in PBS-5% FCS on ice for 30 min with rabbit anti-K10 (1:1000) (Biolegend), and K10 was visualized with anti-rabbit AF488 (Life Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... and transferred to a 2 mL Eppendorf tube for immunostaining for 30 minutes on ice for the immune cells (CD45-PE/Cy7, 1:250, 103114, BioLegend), endothelial cells (ICAM2-A647 ...
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bioRxiv - Immunology 2021Quote: ... cells were washed in staining buffer (1% BGS-supplemented PBS) and incubated with anti-Flag-PE (clone L5; BioLegend #637310) and Ghost Dye Violet 510 (Tonbo Biosciences #13-0870-T100 ...
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bioRxiv - Molecular Biology 2020Quote: ... and c-Kit on mast cells were analyzed by Flow cytometry using fluorochrome labelled antibodies (PE-CY7–conjugated anti-FcεRIα, MAR-1 Biolegend #134317; allophycocyanin-CY7–conjugated anti–c-Kit, 2B8, Biolegend, #105825). The YFP+ cells were FACS-sorted and deletion of the Gata2 gene was determined by PCR to be greater than 99%.
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bioRxiv - Cell Biology 2022Quote: ... 105 - 106 cells were resuspended in FACS buffer with 1 µg/ml TruStain FcXTM anti-mouse CD16/32 antibody (BioLegend) to block for 5 minutes before adding conjugated surface antibodies in FACS buffer at a 2X concentration ...
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bioRxiv - Immunology 2022Quote: ... CD3 localization was detected using FITC conjugated streptavidin (CALTAG Laboratories) and PSGL-1 localization was detected using anti-Rat IgG-PE (BioLegend) following a 30 minute incubation at 4°C ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were enriched via negative selection using biotinylated antibodies at the concentration indicated in Table 1 and using MojoSort Streptavidin Nanobeads (BioLegend). Enrichments were completed in 1X HBSS (Ca2+ and Mg2+ free ...
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bioRxiv - Immunology 2021Quote: Cells were stained with the following surface antibodies: Anti-human CD8a FITC (1:100, clone RPA-T8, BioLegend, cat # 301050), Anti-human TCRgd FITC (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Nunc ELISA plates were coated with 1 μg/ml Spike S1+S2ECD-His recombinant protein (SinoBiologicals) in ELISA coating buffer (Biolegend) overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... isolated single cells were co-stained in FACS buffer with FITC-conjugated mouse anti-human CD45 antibody (1:25; clone HI30, Biolegend), PE-conjugated mouse anti-human CD31 antibody (1:25 ...
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bioRxiv - Immunology 2021Quote: ... 211-11A; Pepro Tech), IL-1β (1 ng/ml, 211-11B; Pepro Tech) or IFNα (100 ng/ml, 752802; Biolegend). Cytokines used in Figure 1 - figure supplement 1D were added at the concertation shown in Supplementary Table 1.
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BET protein inhibition regulates macrophage chromatin accessibility and microbiota-dependent colitisbioRxiv - Immunology 2021Quote: ... LPMCs and splenocytes were washed with cold 1x PBS and stained for viability followed by cell-surface marker staining for CD45 (1:100; Clone 30-F11, BioLegend), CD3ε (1:300 ...
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bioRxiv - Immunology 2021Quote: ... containing fluorescently labelled pMHC tetramers and/or fluorophore-conjugated antibodies (Table) at previously titrated working concentrations (usually 1:200 for commercially available antibodies from BioLegend). After 20 min ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were collected after 45 min incubation at 37 °C and 5 % CO2 and stained with antibodies to microglia cell surface markers (CD11b-PE-Cy7, 1:200, Biolegend, #101216 ...