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Citations for BioLegend :
7551 - 7600 of 8294 citations for 1 6 ANHYDRO β D GLUCOSE 2 3 4 TRI O ACETATE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Cells were first washed with PBS then resuspended in Zombie Aqua Viability Dye (1:500 in PBS, Biolegend). For extracellular staining ...
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bioRxiv - Cell Biology 2023Quote: ... and a 1:500 dilution of Alexa Fluor™ 647 anti-murine Siglec-F (BioLegend, Cat. No. 155519). Depending on the fluorophores selected in each panel ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were then blocked by incubating cells in TruStain Fcx plus Fc block (1:100; BioLegend, 156604) in FACS buffer (DPBS containing 2% FBS and 2mM EDTA ...
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bioRxiv - Immunology 2023Quote: ... cells were re-suspended in PBS and treated with anti-CD16/CD32 (Clone 93, Biolegend, 101301, 1:200) for 15 min on ice ...
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bioRxiv - Immunology 2023Quote: ... cells were centrifuged and resuspended in 1 ml of −20°C cold TruePhos™ Perm Buffer (BioLegend, USA) and incubated at −20°C for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The cell suspension was stained with a panel of antibodies against CD45-Alexa 700 (1:1200; Biolegend; #147716) and CD11b-PE dazzle (1:800 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Dead cells were excluded using the live/dead stain Zombie NIR fixable viability dye (1:2000; Biolegend; #423105). The samples were run on a Cytek® Northern Lights™ cytometer (Cytek biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... The following antibodies were used to assess post-transplant BM reconstitution: CD45.1 FITC (A20, BioLegend 110706; 1:200), CD45.2 Pacific blue (104 ...
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bioRxiv - Immunology 2023Quote: ... and ST2 were determined by flow cytometric analyses using FITC-labeled anti-mouse FcεRIα (clone MAR-1, BioLegend), APC-labeled anti-mouse CD117 (clone 2B8 ...
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bioRxiv - Immunology 2023Quote: ... was added to the wells for 1 hour at RT prior to developing with 3,3’,5,5’-tetramethylbenzidine (TMB) substrate solution (BioLegend). The reaction was stopped by adding 0.1M sulfuric acid and the optical density was determined at 450 nm using a SpectraMax iD5 spectrophotometer.
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bioRxiv - Immunology 2023Quote: ... samples were incubated in 1:200 dilution of donkey antirabbit IgG antibody with Alexa Fluor 488 conjugation (BioLegend) at RT for 30 min (protected from light) ...
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bioRxiv - Immunology 2023Quote: ... Cells were next resuspended in 50 µl (per mouse) lavage buffer containing 0.5 µg biotinylated anti-mouse FcεRIα (clone MAR-1; Biolegend) and incubated 20 minutes at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... pelleted cells were resuspended in MACS buffer containing anti-CD45-FITC (1:1000, clone 30-F11, Biolegend, 103108), PI (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... magnetic activated cell sorting (MACS) was applied using the mouse IgM BG-1 antibody (BioLegend, San Diego, CA) coupled to α-mouse IgM microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted again and resuspended in 100 μl of 1:10 dilution of Human TruStain FcX (Biolegend) in MACS buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were labeled with an antibody cocktail containing Anti-CD14-PE/Cy7 (Clone 63D3, 367111, BioLegend, 1:100), Anti-CD80-APC (Clone 2D10 ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were washed with PBS containing 1% FBS and incubated with APC anti-His Tag Antibody (BioLegend) for 20 minutes on ice ...
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bioRxiv - Bioengineering 2023Quote: ... The following antibodies were mixed and added directly to the cell suspension: BV421 CD86 (1:200, Biolegend, 105031), PacBlue Ly6G (1:150 ...
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bioRxiv - Immunology 2023Quote: ... cells were washed with FACS buffer and stained with goat-anti-mouse IgG-Alexa Fluor647 (1:400, Biolegend) in FACS buffer for 20 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Surface markers were subsequently stained on ice for 20 minutes: anti-mouse CD3-PE (1:200, 17A2, Biolegend), anti-mouse CD4-APC (1:200 ...
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bioRxiv - Genetics 2023Quote: ... proteins were transferred onto nitrocellulose membranes and probed with a 1:2,000 dilution of anti-RpoS antibody (Biolegend). For secondary antibody ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% BSA and 0.07% NaN3 in PBS) with additional Zombie Aqua™ (1:1,000 Fixable Viability Kit, BioLegend) to stain for vital cells ...
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bioRxiv - Developmental Biology 2023Quote: ... then for 1 hour at room temperature (RT) with biotinylated secondary antibodies and finally with fluorochrome-labeled (BioLegend) or peroxidase-labeled streptavidin (Beckman Coulter) ...
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bioRxiv - Immunology 2023Quote: ... stained with cell surface antibodies at 1:1200 (anti-CD8: PerCP-Cyanine5.5, eBioscience, # 45-0081-82, or Biolegend, #100734 ...
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bioRxiv - Cell Biology 2023Quote: ... neurons were incubated live with 10 µg/ml of β1-integrin antibody clone HM β1-1 (102202, Biolegend) for 15 min at 37 °C ...
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bioRxiv - Bioengineering 2022Quote: ... 25 μL of fluorescent counting beads (1 million/mL, 424902, Precision Count Beads, BioLegend, San Diego, California USA) were mixed with 100 μL of cell suspension for each condition ...
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bioRxiv - Neuroscience 2023Quote: ... and SMI-32 (using mouse Purified anti-Neurofilament H nonphosphorylated antibodies, 1:12000 dilution; Biolegend, San Diego, CA) immunohistochemistry (note that only sections adjacent to every 3rd Nissl-stained sections were processed for calbindin or SMI-32 ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 million cells were incubated for 20 min at room temperature with Zombie viability dye (Biolegend ref 77143). Then cells were incubated in PBS + 10% FBS to block Fc receptors ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies that were used in this study included rabbit anti- human CD271 (1:100, HPA004765, Biolegend), mice anti-human CXCL12 (1:50 ...
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bioRxiv - Immunology 2022Quote: ... The c-kit+ fraction was stained with antibodies against CD117 (c-kit APC, clone 2B8, Biolegend, 1/100), Sca-1 (Pacific Blue ...
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bioRxiv - Microbiology 2023Quote: ... gonorrhoeae (1 x 108 CFU) was incubated with 1.5 µl Tag-IT® Violet (TIV) (Biolegend, Catalog # 425101) in 100 µl 1X PBS + 5 mM MgSO4 ...
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bioRxiv - Immunology 2024Quote: ... sections were stained for 3h at room temperature with anti-CD3 PE-Dazzle594 (Biolegend, Cat: 100245, 1:50), anti-CD4 PE (Biolegend ...
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bioRxiv - Physiology 2024Quote: ... followed by 30-min incubation with the following diluted antibodies: BV605-anti-mouse CD45 (BioLegend #103139, 1:200), BV421-anti-mouse Ly6G (BioLegend #127627 ...
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bioRxiv - Immunology 2023Quote: ... and 1:20 dilution mouse anti-human CD27-PE (stock 50 ug/mL, BioLegend, San Diego, CA, USA), which has been reported as a marker for juvenile B cells ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were pre-stained with Fc blocker for 10 min in ice followed by staining at 1:300 with anti-CD64 (139304, PE anti-mouse CD64, clone:X54-5/7.1, BioLegend) and anti-Pdgfra (25-1401-82 ...
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bioRxiv - Immunology 2023Quote: ... anti-mouse CD279 (PD-1) (clone: RMP1-30, PE, Biolegend, cat# 109104; clone: RMP1-30, PE-Cy7, Biolegend, cat# 109110 ...
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bioRxiv - Biochemistry 2023Quote: ... using the following reagents: anti-HA (HA.11 Clone 16B12 Monoclonal antibody, BioLegend 901514, used at 1:1,000), Alexa Fluor 680 donkey anti-rabbit IgG(H+L ...
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bioRxiv - Bioengineering 2023Quote: ... were prepared in 1× Annexin V Binding Buffer (100 mM HEPES, 25 mM CaCl2, 1.4 M NaCl; BioLegend). To analyze cells ...
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bioRxiv - Cancer Biology 2023Quote: ... The single-cell suspensions were incubated in mouse Fc block (anti-CD16/32) antibody (Biolegend, 101335, 1:300) and then stained with the appropriate antibodies in FACS Buffer as described previously [33] ...
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bioRxiv - Immunology 2023Quote: ... kidneys were washed three times for 1 hr at room temperature in Ce3D™ Wash Buffer (427710, Biolegend), then cut in half and transferred to Ce3D™ Permeabilization/Blocking Buffer (48 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... A far-red BST2 antibody was used to label BST2-expressing cells (diluted 1:40, BioLegend, cat. 348416). Cell viability was determined by Sytox Blue (diluted 1:1000 ...
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bioRxiv - Immunology 2023Quote: ... sorted TN and TEMRA cells were stimulated with plate-bound anti-human CD3 (OKT3; 1 μg/ml; BioLegend) and anti-human CD28 (CD28.2 ...
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bioRxiv - Developmental Biology 2023Quote: ... The grids were incubated with mouse anti-HA.11 epitope tag antibody (BioLegend, 901501, 1:1000, RRID: AB_2565006) for 30 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Viability staining was performed using Zombie NIR for 10 minutes at room temperature (1:500 in PBS, BioLegend). Cells were pelleted and resuspended in anti-CD16/32 antibody (1:200 ...
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bioRxiv - Immunology 2023Quote: ... to block Fc receptors followed by staining with fluorochrome-conjugated anti–CD11b (1:200 dilution, BioLegend, no. 101226), anti–Ly6C (1:200 dilution ...
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bioRxiv - Immunology 2023Quote: ... approximately 1.0×106 PBMCs were firstly incubated with LIVE/Dead Zombie Aqua Dye (77143, BioLegend; 1 μL/well) for 15min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with PBS prior to staining with Zombie NIR Fixable live/dead dye (1:500) (Biolegend) for 20 min at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The level of CTLA4 was measure by intracellular staining using anti-CTLA4 (Biolegend, Cat#106311, 1:1000 dilution) and the Transcription Factor Staining Buffer Set (eBioscience ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 100 μL Zombie NIR (1:1000 in PBS, Zombie NIR™ Fixable Viability Kit, Biolegend, #423106), transferred into a 96-well plate and incubated for 20 min at 4 °C in the dark ...
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bioRxiv - Immunology 2024Quote: ... 5 μl PE-labeled mouse anti-human CD11b fluorescent antibody (cat. no. 301306; 1:1,000 dilution; BioLegend, Inc.) or FITC-labeled mouse anti human CD14 fluorescent antibody (cat ...