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Citations for BioLegend :
701 - 750 of 4942 citations for Rat Neural Stem Cell Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... The slides were then incubated in the primary antibodies (rat anti-PECAM-1, clone 390, EMD Millipore; rabbit anti-NG-2 chondroitin sulfate, EMD Millipore; rabbit anti-Keratin-14, BioLegend) diluted 1:100 in the blocking solution overnight at 4 °C ...
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bioRxiv - Immunology 2019Quote: ... 2 ml medium/well) were cultured in growth media supplemented with GM-CSF (100 ng/ml, BioLegend, San Diego), TGF-β (10 ng/ml ...
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bioRxiv - Immunology 2019Quote: TNF-α and IL-6 were measured in the media of activated BMDMs using ELISA kits purchased from Biolegend per manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... They were cultured in Th1-polarising media consisting of R10 with 1 μg/mL anti-mouse CD28 (102101, BioLegend), 5 μg/mL anti-mouse IL-4 (504102 ...
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bioRxiv - Immunology 2024Quote: ... purified ILC2 isolated from IL-33-challenged mice were cultured overnight with 10μM TOFA in complete media supplemented with 20 ng/ml IL-7 (Biolegend). Cells were washed with assay medium supplemented with 10 mM glucose ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PE-Cy7 conjugated anti-rat CD11b/c (clone OX-42, Cat#201818), APC-Cy7 conjugated anti-rat CD4 (clone W3/25, Cat#201518) from Biolegend (San Diego, USA), and AF647-conjugated anti-rat RT1B (clone OX-6 ...
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bioRxiv - Immunology 2019Quote: ... Cell suspensions were incubated for 10 minutes at 4°C with 10μg/ml Fc Block (purified rat anti-mouse CD16/CD32, Biolegend TruStain FcX anti-mouse) to block non-specific binding of antibodies to Fc Receptors and then incubated at 4°C for 30 minutes with anti-CD45-PE (1:300 ...
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bioRxiv - Immunology 2019Quote: Cells were incubated for 10 minutes at 4°C with 10μg/ml of purified rat anti-mouse CD16/CD32 (Biolegend TruStain FcX anti-mouse) to block non-specific binding of antibodies to Fc Receptors and then incubated at 4°C for 30 minutes with Fixable Viability Staining e780 and fluorochrome/biotin conjugated antibodies (purchased from BD Bioscience ...
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bioRxiv - Cell Biology 2022Quote: ... Control antibodies were biotin-conjugated rat immunoglobulin (Ig) G1 (5 μg/mL; eBioscience, San Diego, CA) and PE/Cy7-conjugated rat IgG1 (5 μg/mL; BioLegend, San Diego, CA). Flow cytometry data were collected using Fortessa LSRII (Becton Dickinson ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were then stained overnight at 4 °C in a humidified chamber with rat monoclonal anti-mouse CD68 (1:400, BioLegend, clone FA-11) and rabbit polyclonal anti-mouse IL-10 (1:25 ...
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bioRxiv - Immunology 2022Quote: ... Frozen PBMC of post-CA patients were thawed and incubated in media (RPMI1640 + 2% FBS) with recombinant human Nectin-2 (Biolegend) or isotype control (ENZO ...
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bioRxiv - Bioengineering 2022Quote: ... Cytokine-primed BMDMs were cultured in RPMI growth media + 20 ng/mL M-CSF + 20 ng/mL IFNγ (BioLegend 575302) or 20 ng/mL IL-4 (BioLegend 574302 ...
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bioRxiv - Genetics 2023Quote: ... Lin-Sca-1+ c-Kit+ Flt3-CD150+ CD48-on a FACSAria into StemSpan SFEM II media with SCF (100 ng/ml, BioLegend), TPO (50 ng/ml ...
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bioRxiv - Neuroscience 2020Quote: The cerebellar slices were treated either 3 times 24 hours apart with combinations of recombinant TNF (recombinant rat TNF, Biolegend, San Diego, CA, USA), LTα (recombinant human LT-α ...
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bioRxiv - Neuroscience 2022Quote: ... APC-Cy7 rat anti-mCD45 (1:200, Becton Dickinson, Franklin Lakes, NJ, USA) and APC-Cy7 anti-mTer119 (1:100, Biolegend, San Diego, CA, USA). In order to sort neural stem and precursor cells ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were stained with unconjugated rat anti-mouse lineage-specific antibodies (Ter-119, Mac1, Gr-1, B220, CD5, CD3, CD4, CD8, and CD127, Biolegend, San Diego, CA, USA), followed by incubating with Dynabeads anti-rat IgG (Thermo Fisher) ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... The slides were washed with PBS and incubated for 4 hours at room temperature with rat anti- mouse Ly6G (cone 1A8, BioLegend, San Diego, CA, USA). Slides were washed with PBS and incubated separately with Alexa Fluor 568 goat anti-rabbit (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Sections were then stained with primary antibody diluted in 1% BSA in PBS for 1 hour with rat anti CD68 (BioLegend 137002 1:400 dilution), rabbit anti Lyve1 (abcam ab14917 1:200 dilution) ...
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bioRxiv - Bioengineering 2024Quote: ... The sections were subsequently stained with a rat anti-human YAP antibody conjugated with PE (dilution: 1:500; clone W19260A; Biolegend, San Diego, CA, USA) for 2 hours at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... mice were intravenously injected with 15 μg of rat anti-mouse CD31 coupled to Alexa-Fluor-647 (clone 390, Biolegend, San Diego, CA, United States) approximately 2 hr prior to imaging.
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bioRxiv - Neuroscience 2021Quote: ... or monoclonal fluorescent conjugated antibodies (rat anti-mouse CD3, clone 17A2; mouse anti-mouse CD45.2, clone 104; all from Biolegend and used at 0.4mg/kg i.v.).
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bioRxiv - Immunology 2021Quote: ... cells were split into 2 new 96-well plates and incubated with freshly prepared supplemented RPMI media with IL-2 (10 ng/ml, BioLegend, #575402). Cells were cultured for a total of 5 days before injection ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were blocked using DMEM/FBS media for 1 hour at RT followed by incubation with 1:1000 dilution mouse anti-HA (Biolegend, 901514) and rabbit anti-PCNA (Cell Signaling ...
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bioRxiv - Developmental Biology 2021Quote: ... and transferred to 2 ml tubes for incubation with the following antibodies diluted in media containing FBS: CD45-PE/Cy7 (BioLegend, 103114), ICAM2-A647 (Life tech ...
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bioRxiv - Neuroscience 2023Quote: ... Conditioned media was run in duplicate using an IL-1α ELISA (ELISA MAX Deluxe Set Mouse IL-1α, BioLegend catalog # 433404).
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bioRxiv - Microbiology 2022Quote: ... Quantification of cytokine and chemokine levels in culture media was performed using the LEGENDPlex kit from BioLegend (human anti-virus response panel), according to the manufacturer’s recommendations.
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bioRxiv - Microbiology 2020Quote: ... CD19+ and CD4− CD8− CD19− cells were resuspended in R-10 media (RPMI + 2 mM L-glutamine + 10% FBS + Penicillin/streptomycin) + 20 U/ml IL-2 (Biolegend, https://www.biolegend.com). Primary monocytes were resuspended in R-10 media ...
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bioRxiv - Bioengineering 2023Quote: ... T-cells that have been incubating with conditioned media were carefully transferred to the CD3-coated plates and further stimulated with 1 µg/mL anti-CD28 (BioLegend, clone: CD28.2) for another 48 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were passed through 70-micron cell strainer and subsequently cultured 10mL complete RPMI media supplemented with 20 ng/mL of recombinant murine M-CSF (576406; BioLegend, 315-02 PeproTech). On day 3 ...
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bioRxiv - Immunology 2019Quote: ... Samples were incubated overnight at 4°C in primary antibody diluted in blocking buffer (R&D AF-400-NA goat anti-IL-1 α, 1:50; Novus Biologicals NB600-408 rabbit anti-collagen I, 1:50; Biolegend 103101 rat anti-CD45, 1:50). The next morning ...
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bioRxiv - Immunology 2022Quote: ... cells were stimulated using Cell Activation cocktail (Biolegend) for 4h at 37°C in RPMI containing 10% FBS ...
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bioRxiv - Immunology 2021Quote: Peripheral blood mononuclear cells (Veri-Cells PBMC, BioLegend) were stained with each novel fluorochrome for 30 minutes and fixed with various fixatives according to the manufacturer’s instructions – Cell Staining Buffer (PBS + 10% Fetal Bovine Serum (FBS)) ...
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bioRxiv - Physiology 2023Quote: ... Cells were washed with cell staining buffer (Biolegend), and non-specific binding was blocked with TruStain FcX Plus (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... cells were resuspended in Cell Staining Buffer (BioLegend) containing Fc receptor blocker (Miltenyi Biotec ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed with Cell Staining Buffer (BioLegend) and stained with a cocktail of surface antibodies for 20 minutes at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... Cell pellets were resuspended in cell staining buffer with zombie aqua cell viability stain (BioLegend), were incubated at 4 °C for 15 minutes ...
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bioRxiv - Pathology 2022Quote: ... Cells (0.5–4.0 × 106 viable cells) were then resuspended in cell staining buffer (#420201, BioLegend) and incubated with TruStain FcX ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells were washed in Cell Staining Buffer (Biolegend) and stained with TotalSeq HTO antibodies for 30 min on ice ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were stained with Zombie Aqua cell dye (BioLegend) for 15 min at a 1:200 dilution in cell staining buffer (BioLegend) ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were washed with Cell Staining Buffer (Biolegend, #420201), then washed ...
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bioRxiv - Microbiology 2023Quote: ... lung cells were incubated with cell activation cocktail (Biolegend) and monensin for 4 h ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were stained with cell-hashing antibodies (TotalSeq, BioLegend) before cell capture ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were washed once with Cell Staining Buffer (Biolegend) prior to cell counting and antibody staining ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were stained for dead cells (Zombie NIR; Biolegend) and surface markers (CD4 and CD8 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed three times with cell staining buffer and resuspended in Cell Staining Buffer (BioLegend) prior to flow cytometry using a MACSQuant Analyzer (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant containing the dead cells was aspirated and cells were resuspended in Cell Staining Buffer (BioLegend).
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bioRxiv - Neuroscience 2022Quote: ... 1×106 cells from each iPS cell line were resuspended in 45 μL cell staining buffer (BioLegend) in a protein low-binding microcentrifuge tube separately and incubated with 5 μL Fc blocking reagent (BioLegend ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were harvested for cell cycle analysis using the Phase-Flow BrdU Cell Proliferation Kit (Biolegend, USA). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... and the cell pellet was treated to remove red cells (Red blood cell lysis buffer, 420301, Biolegend). After a wash ...
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bioRxiv - Cell Biology 2023Quote: ... Cell-hashing was performed by incubating 1X106 cells with Total-SeqB (B0301-B0304) cell-hashing antibodies (Biolegend) for 30 minutes ...