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Citations for BioLegend :
651 - 700 of 4942 citations for Rat Neural Stem Cell Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... for 24h at 4°C on a shaker and then stained with antibodies (rat anti-CD3 [clone 17A2, cat#100202, Biolegend] ...
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bioRxiv - Molecular Biology 2022Quote: ... Alexa Fluor® 488-conjugated or PE/Cy7-conjugated rat anti-mouse CD326 (EpCAM) antibody (1:200, BioLegend, clone G8.8), Alexa Fluor® 647-conjugated rat anti-mouse CD104 (integrin β4 ...
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bioRxiv - Physiology 2022Quote: ... Samples were incubated with anti-c-kit rat monoclonal antibody (1:500 v/v, ACK2, BioLegend, San Diego, CA, USA) overnight (4ºC) ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were washed again 3 × 5 minutes in PBS and incubated overnight with rat anti-phospho-TDP primary antibody (1:100, 829901, BioLegend), in a dilution buffer (0.05% Tween ...
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bioRxiv - Immunology 2020Quote: ... Randomized NOD/SCID mice were then administered the following treatments for two weeks post-adoptive transfer: (i) PBS and rat isotype control IgG2b antibodies (RTK4530; BioLegend), (ii ...
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bioRxiv - Immunology 2020Quote: ... the cremaster muscles were stained by using primary antibodies including rabbit anti-mouse laminin α5 (antibody 405) or anti-mouse laminin α4 (antibody 377) and Brilliant Violet 411-conjugated rat anti-mouse MHC II (Biolegend); monocytes were identified by CX3CR1GFP expression ...
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bioRxiv - Cell Biology 2021Quote: ... Unfractionated or c-Kit-enriched BM cells were then incubated with purified rat anti-mouse lineage antibodies (CD3, BioLegend, 100202; CD4, eBioscience, 16-0041-82; CD5, BioLegend, 100602; CD8, BioLegend, 100702 ...
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bioRxiv - Cell Biology 2021Quote: ... Unfractionated or c-Kit-enriched BM cells were then incubated with purified rat anti-mouse lineage antibodies (CD3, BioLegend, 100202; CD4, eBioscience, 16-0041-82; CD5, BioLegend, 100602 ...
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bioRxiv - Immunology 2020Quote: ... washed with FACS buffer and then stained with a secondary goat anti-rat IgG DyLight 649 (BioLegend Cat# 405411, RRID:AB_1575141) antibody and then washed with FACS buffer and blocked with 5% normal rat serum ...
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bioRxiv - Immunology 2021Quote: ... and finally with a mixture of AlexaFluor 647-conjugated rat anti-mouse Ly6C antibody (BioLegend, clone HK1.4; diluted 1:500) and DAPI (Molecular Probes ...
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bioRxiv - Cell Biology 2022Quote: ... were performed after confirming full differentiation into mature MCs using flow cytometric analysis of lineage-specific c-KIT (rat anti-CD117 Brilliant Violet 421-conjugated, 1:200, Biolegend) and FcεRI (armenian hamster anti-FcεRIα Alexa Fluor 647-conjugated ...
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bioRxiv - Cell Biology 2022Quote: ... Full differentiation into mature MCs was confirmed by expression of lineage-specific c-KIT (rat anti-CD117 Brilliant Violet 421- conjugated, 1:200, Biolegend) and FcεRI (armenian hamster anti-FcεRIα Alexa Fluor 647- conjugated ...
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bioRxiv - Neuroscience 2023Quote: ... For further experiments viable CD45lowSiglec-H+ microglia were labeled with rat anti-ICAM1 BV711 (1:100, catalog no. 116143; BioLegend), rat anti- Ly6A/E FITC (1:100 ...
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bioRxiv - Physiology 2023Quote: ... single cell suspension from mouse esophagi was incubated at 37°C for 30 minutes with Integrin β1 antibody (PE anti-mouse/rat CD29; 102207; Biolegend) at 1:200 dilution ...
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bioRxiv - Physiology 2023Quote: ... the resulting single cell suspension was incubated at 37°C for 30 minutes with Integrin β1 antibody (APC anti-mouse/rat CD29; 102216; Biolegend) at 1:100 dilution ...
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bioRxiv - Immunology 2024Quote: ... and a secondary antibody goat anti-mouse antibody conjugated with APC or rat anti-mouse antibody conjugated with Alexa Fluro 700 (BioLegend). These stained cells were fixed with fixation buffer (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... and HRP-conjugated goat anti-mouse secondary antibody (1:3750 dilution) or a purified rat anti-SARS-CoV-2 spike protein S1 antibody (1:1000 dilution, BioLegend) and HRP-conjugated goat anti-rat secondary antibody (1:3000 dilution ...
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bioRxiv - Immunology 2022Quote: ... media was supplemented with 2 μg/ml of mouse IgG1 K anti-human PD-1 antibody (Biolegend) either alone or in combination with 1 μg/ml mouse IgG2 B anti-human TIGIT (R&D systems ...
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bioRxiv - Biochemistry 2020Quote: Conditioned media samples were analyzed using commercially available ELISAs as per manufacturer guidelines (BioLegend, San Diego, CA), at 2X and 8X dilutions for IL-6 ...
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bioRxiv - Cancer Biology 2022Quote: ... 600 μL of normal growth media containing 0 or 250 ng/mL CCL4 (BioLegend, cat no. 554602) was added to the lower chamber ...
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bioRxiv - Cell Biology 2019Quote: ... EPO level was measured in the conditioned media using the LEGEND MAX Human Erythropoietin ELISA kit (Biolegend), according to manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... aliquots of conditioned media were collected for measuring IL-2 and IFNγ by ELISA (ELISA MAX, BioLegend). Cells were washed and stained with anti-mouse CD3ε-FITC (145-2C11 ...
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bioRxiv - Physiology 2023Quote: ... then seeded in 2mL of conditioned media with macrophage colony-stimulating factor (mCSF, 25 ng/mL, BioLegend). Five days later ...
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bioRxiv - Developmental Biology 2021Quote: ... The following primary antibodies were used for immunostaining: rat anti-EpCAM-AF647 (Biolegend, clone G8.8, 1 µg ml−1, 1:250); rabbit anti-K5 (Covance ...
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bioRxiv - Immunology 2019Quote: ... from BD Sciences (San Jose, CA); Alexa Fluor® 647-conjugated anti-rat Thy1 (OX-7, #202508; RRID:AB_492884) from BioLegend (San Diego, CA). Ghost Dye™ Violet 450 was from Tonbo Biosciences (#13-0863-T100 ...
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bioRxiv - Cell Biology 2021Quote: ... and the secondary Alexa Fluor donkey 488-conjugated anti-mouse IgG pAb (Thermo Fischer Scientific, USA) and the Alexa Fluor 667-conjugated rat anti-mouse CD31 mAb (clone MEC133, Biolegend, USA).
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bioRxiv - Cell Biology 2020Quote: ... day-17 organoids were dissociated as described above and incubated with Alex Fluor 647-conjugated monoclonal rat anti-CD49f (BioLegend, CA) and FITC-conjugated monoclonal mouse anti-CD26 (BioLegend ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: Approximately 1×106 cells were incubated with the following antibodies in order to characterize spleen immune subpopulations: Alexa Fluor® 488-conjugated-anti rat CD3 (IF4, Biolegend), PE Cy5-conjugated-anti rat CD4 (biolegend) ...
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bioRxiv - Genetics 2020Quote: Immunoprecipitation and immunoblotting were carried out according to standard protocols with the following antibodies: rat anti-Flag L5 antibody (BioLegend; 637301), rabbit anti-GFP antibody (Novus ...
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bioRxiv - Cell Biology 2022Quote: ... 45-5981-82), hamster anti-mouse CD48 APC (eBioscience, 17-0481-82) and rat anti-mouse CD150 PE-Cy7 (Biolegend, 115914). To examine macrophages ...
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bioRxiv - Immunology 2023Quote: ... PE-anti-PD-1 (clone RMP1-30, 1:20) and its isotype control PE-Rat IgG2b,κ (clone RTK4530, 1:20) were purchased from Biolegend. PE-anti-PD-L1 (clone MIH5 ...
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bioRxiv - Cancer Biology 2023Quote: The antibody mix for detection of MDSCs contained a rat anti-mouse CD45 (30-F11) antibody conjugated to PE (Biolegend; 103106), a rat anti-mouse Ly-6G/Ly-6C (Gr1 ...
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bioRxiv - Cancer Biology 2023Quote: The antibody mix for CTC detection of human CTCs in animal models contained a rat anti-mouse CD45 (30-F11) antibody conjugated to PE (Biolegend; 103106) and mouse anti-human HLA conjugated to AlexaFluor 647 (Biolegend ...
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bioRxiv - Bioengineering 2023Quote: ... Cells pre-treated with anti-mouse TfR-1 antibodies (human constant regions) were incubated with 50 nM of a second TfR-1 antibody for detection (R17, rat anti-mouse antibody; BioLegend, 113802). Cells pre-treated with anti-mouse CD98hc antibodies (human constant regions ...
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bioRxiv - Bioengineering 2023Quote: ... Cells pre-treated with anti-mouse CD98hc antibodies (human constant regions) were incubated with 10 nM of a second CD98hc antibody for detection (RL388, rat anti-mouse antibody; BioLegend, 128202). Non-treated cells were also incubated with the TfR-1 (R17 ...
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bioRxiv - Cell Biology 2024Quote: ... all input solutions were supplemented with 1 μg/mL of PE-conjugated rat anti-mouse TNFα antibody (MP6-XT22, BioLegend, UK).
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bioRxiv - Cancer Biology 2023Quote: The antibodies used for flow staining in this study are: rat anti-mouse/human CD11b conjugated to BV605 (clone M1/70, Biolegend, 101257), hamster anti-mouse CD3e conjugated to BV510 (clone 145-2C11 ...
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bioRxiv - Immunology 2024Quote: Naïve or PR8 infected B6 mice were administered daily 20µg anti-CCR2 depleting antibodies (MC-21 generously provided by Prof. Matthias Mack, Universität Regensburg) or rat IgG2b,κ isotype control (Biolegend, #400644) intraperitoneally (i.p.) ...
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bioRxiv - Immunology 2022Quote: ... The concentration of CXCL10 in the media was determined using a Human CXCL10 ELISA MAX kit (BioLegend 439904).
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bioRxiv - Physiology 2022Quote: ... Organoids were harvested in cold Serum-Free Defined Medium media and incubated with Zombie (BioLegend 423107, 1:100) for 15 minutes in the dark at room temperature ...
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bioRxiv - Immunology 2023Quote: ... and plated in TRM-like culture media with 10 ng/mL mouse IL-7 (Biolegend Catalog No. 577804) and 10 ng/mL human TGFβ1 (Fisher Scientific PHG9214 ...
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bioRxiv - Cancer Biology 2023Quote: ... and supplemented with sCTLA-4 conditioned media or murine CTLA-4-Fc fusion protein (10 µg/mL, BioLegend). After 4 days ...
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bioRxiv - Cancer Biology 2024Quote: ... Aliquotes of the same media were used for quantification of cytokines using the Legendplex cytometric bead assay (Biolegend) as described above.
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bioRxiv - Neuroscience 2021Quote: ... The exception is that secondary labeling was accomplished using anti-rat DyLight 405 (1:5000, Novus, NBPI-72983) or anti-mouse DyLight 405 (1:1000, BioLegend, Poly-24091). Fixed cells were then imaged on a Nikon Eclipse TE2000 inverted microscope using a 60x 1.49NA oil-immersion TIRF objective and 405nm and 560nm solid lasers using a CoolSNAP HQ2 CCD camera ...
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DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: To measure the calprotectin/MRP 8/14 from the fecal samples, S100A8/S100A9 ELISA kit rat/mice (#K6936, Immundiagnostik AG, Germany) as well as LEGENplex™ (#740150 or #740446, Biolegend, Germany) mouse inflammation panel (13-plex ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... A custom rat multi-analyte LEGENDplex bead-based immunoassay kit was used to examine cytokine concentrations in plasma samples (LEGENDplex; BioLegend, CA, USA) that targeted six cytokines concurrently (IL-6 ...
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bioRxiv - Cell Biology 2023Quote: ... the pellet was resuspended in PBS and incubated for 30 min at 4 °C with rat FITC-conjugated mAb against CD11b (1:200, BioLegend, CA, USA). After incubation ...
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bioRxiv - Immunology 2023Quote: ... PE rat IgG2a-isotype control antibody and PE mouse IgG2b-d κ isotype control antibody were purchased from BioLegend (Sandiego, CA, USA) and PE mouse IgG1-κ Isotype control antibody from BD Biosciences ...
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bioRxiv - Bioengineering 2023Quote: ... the serum samples collected at 6 hours and 48 hours after administration were diluted 2-fold for ELISA analysis using the LEGEND MAX™ Rat IL-6 ELISA Kit with pre-coated plate (Biolegend, USA) according to the manufacturer’s recommendations with a little modification as described below ...
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bioRxiv - Bioengineering 2023Quote: ... The slides were then incubated in the primary antibodies (rat anti-PECAM-1, clone 390, EMD Millipore; rabbit anti-NG-2 chondroitin sulfate, EMD Millipore; rabbit anti-Keratin-14, BioLegend) diluted 1:100 in the blocking solution overnight at 4 °C ...