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Citations for BioLegend :
7001 - 7050 of 9316 citations for 8 11 Methano 10a 3 6a 1 propanyl 3 ylidene 8H indeno 2 1 b azocine 12 14 dione 5 acetyloxy 4 benzoyloxy dodecahydro 1 3 dimethyl 9 methylene 3R 4S 5R 6aR 6bR 8S 10aR 11R 11aS 15S 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... CD3 localization was detected using FITC conjugated streptavidin (CALTAG Laboratories) and PSGL-1 localization was detected using anti-Rat IgG-PE (BioLegend) following a 30 minute incubation at 4°C ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were enriched via negative selection using biotinylated antibodies at the concentration indicated in Table 1 and using MojoSort Streptavidin Nanobeads (BioLegend). Enrichments were completed in 1X HBSS (Ca2+ and Mg2+ free ...
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bioRxiv - Immunology 2021Quote: Cells were stained with the following surface antibodies: Anti-human CD8a FITC (1:100, clone RPA-T8, BioLegend, cat # 301050), Anti-human TCRgd FITC (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Nunc ELISA plates were coated with 1 μg/ml Spike S1+S2ECD-His recombinant protein (SinoBiologicals) in ELISA coating buffer (Biolegend) overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... isolated single cells were co-stained in FACS buffer with FITC-conjugated mouse anti-human CD45 antibody (1:25; clone HI30, Biolegend), PE-conjugated mouse anti-human CD31 antibody (1:25 ...
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BET protein inhibition regulates macrophage chromatin accessibility and microbiota-dependent colitisbioRxiv - Immunology 2021Quote: ... LPMCs and splenocytes were washed with cold 1x PBS and stained for viability followed by cell-surface marker staining for CD45 (1:100; Clone 30-F11, BioLegend), CD3ε (1:300 ...
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bioRxiv - Immunology 2021Quote: ... containing fluorescently labelled pMHC tetramers and/or fluorophore-conjugated antibodies (Table) at previously titrated working concentrations (usually 1:200 for commercially available antibodies from BioLegend). After 20 min ...
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bioRxiv - Immunology 2019Quote: ... The slides were blocked with 5% FBS in PBS for 1 hour at room temperature before surface staining with APC conjugated anti-CD45 (clone 30-F11; Biolegend) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... Human B cells were incubated in human Fc receptor blocking antibody then stained with Zombie Aqua fixable viability dye (1:500 dilution) (BioLegend), according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2019Quote: ... MCF-7 cells in EPB were stained with a 1:20 dilution of FITC Anti-human CD326 (Biolegend, Cat.no. 324204) for 30 min at 4 °C to allow for population tracking in flow cytometry ...
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bioRxiv - Immunology 2019Quote: ... (IRF4:rat monoclonal, eBiosciences, UK, mouse monoclonal: IRF8, eBiosciences, BATF, R&D Systems, JUN Millimark, UK, PU.1 Biolegend, UK). IRF1 staining was done using rabbit polyclonal anti-human IRF1 antibody (Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... LT-HSCs were stained with conjugated γH2AX antibody (FITC-conjugated anti-phospho-H2A.X antibody, Biolegend, clone 2F3, 1:50 dilution) for 2 hrs at 37°C ...
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bioRxiv - Biophysics 2020Quote: ... Platelets were fixed with 1% formalin in phosphate buffered saline and incubated with phycoerythrin-labelled anti-CD62P (P-selectin; Biolegend) antibody following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Wells were cultured for 1 hour in a 10:1 ratio prior to being washed and stained with fluorochrome-conjugated Abs: anti-CD41 (clone MWReg- Biolegend) and anti-CD8 (clone 53.67- Biolegend) ...
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bioRxiv - Immunology 2021Quote: CD3+ T cells from lung single-cell suspensions (1×107 cells) were isolated by negative selection using the MojoSort mouse CD3+ T-cell Isolation kit (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... A maximum of 1×106 cells/donor were stained by oligo-barcoded antibodies (TotalSeq-A or TotalSeq-C; Biolegend, Expedeon) for 30 min on ice ...
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bioRxiv - Bioengineering 2020Quote: ... the single cell pellets were resuspended in 1mL FACS buffer containing 1:300 dilution of anti-EpCam-AF467 (Biolegend, 118211) (for lung and kidney epithelial cell ...
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bioRxiv - Bioengineering 2020Quote: ... Staining buffer used was a 1:1000 dilution of stock antibody solution (APC anti-mouse CD45; Alexa Fluor 488 anti-mouse Ly6G, Biolegend) into FACS buffer ...
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bioRxiv - Neuroscience 2021Quote: ... CD8+ T cells and CD45/CD11b+ cells (macrophages/microglia) was performed using the following antibodies in a 1:200 dilution: anti-CD3e (clone 145-2C11, BioLegend), anti-CD4 (clone GK 1.5 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with 1.5% normal serum followed by incubation with the primary antibody (biotinylated anti-Aβ – 1:100, BioLegend, USA) for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysates were cleared by centrifugation and β1 integrin was immunoprecipitated with anti-β1 integrin (clone HMb1-1, BioLegend, #102202) and Dynabeads Protein G (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Tissue sections were then washed and stained with an AF647-directly conjugated antibody towards CCR6 (1:200, clone G034E3, BioLegend) at 37 ° C for one hour ...
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bioRxiv - Immunology 2020Quote: A total of 1 x 106 mononuclear cells suspended in PBS were stained using fixable viability dye (Zombie NIR-Biolegend), and incubated for 15 minutes at room temperature following manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2020Quote: ... Immunostaining of specific receptors was performed by incubating cells overnight with AlexaFluor 647 conjugated primary antibodies (human CD4: OKT4, dilution: 1:100, source: Biolegend; human CD45 ...
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bioRxiv - Cell Biology 2020Quote: ... Measurements were compared to the isotopic control (APC-conjugated anti-mouse IgG1, Biolegend clone MOPC-21 #BLE400122, 1/100, Ozyme) to determine background and positivity thresholds ...
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bioRxiv - Cancer Biology 2021Quote: ... For the assessment of CD71 expression in TF-1 and DAMI cells were labeled with the phycoerythrin (PE)-cyanine 7 (Cy7)-conjugated CD71 (BioLegend) (#113811) ...
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bioRxiv - Immunology 2022Quote: ... the processed fecal samples are incubated with autologous diluted serum and stained as previously described with the following fluorophore-conjugated antibodies: goat anti-mouse PE-IgA and PE-Cy7-IgG1 (clone RMG1-1; Biolegend). Flow cytometry analysis on stained bacteria populations was performed on the LSRFortessa and data analysis was performed on FlowJo ver ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were next incubated in 1x BD Perm/Wash buffer containing AF674-conjugated anti-Ki67 antibody (16A8; Biolegend; 1:100) at 4°C overnight ...
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bioRxiv - Immunology 2022Quote: ... T cells were washed in PBS/1% FCS and stained using the following mAbs in appropriate combinations: anti-OX40-PE-Cy7 (BioLegend), anti-GITR- BV421 (BioLegend) ...
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bioRxiv - Biophysics 2022Quote: ... were established by transfection of pIRES-HA-CXCR4 and pIRES-HA-CXCR4-W1955.34L and subsequent cell sorting for equivalent surface expression levels using 1:1000 Alexa Fluor 488-labeled anti-HA antibody (clone 16B12, Biolegend). U87 cells were grown in Dulbecco’s modified Eagle medium (Themo Fischer Scientific ...
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bioRxiv - Biophysics 2022Quote: ... Equivalent surface expression level was verified using an Alexa Fluor 488-conjugated anti-HA-tag mAb (clone 16B12, 1:1000 Biolegend). Flow cytometry data were analyzed using FlowJo V10 software.
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bioRxiv - Immunology 2022Quote: ... The following antibodies were used to identify MKs: 1:100 anti-mouse CD41-FITC+ and anti-mouse CD42d-APC+ (BioLegend); and MKPs ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with primary antibodies (anti-GFAP, DAKO, z-0334, 1:1000; SMI-99 [anti-myelin basic protein] Biolegend; 808401 ...
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bioRxiv - Immunology 2022Quote: ... The clone and dilution of antibodies used for flow cytometry were as follows: CD4-Pacific Blue (Biolegend, OKT4, 1:200), CD69-APC (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... the cells were stained with a live/dead fixable dye (Zombie Red dye, 1/800 dilution – BioLegend, San Diego, CA) for 20 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Alexa Fluor® 488-conjugated or PE/Cy7-conjugated rat anti-mouse CD326 (EpCAM) antibody (1:200, BioLegend, clone G8.8), Alexa Fluor® 647-conjugated rat anti-mouse CD104 (integrin β4 ...
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bioRxiv - Physiology 2022Quote: ... Samples were incubated with anti-c-kit rat monoclonal antibody (1:500 v/v, ACK2, BioLegend, San Diego, CA, USA) overnight (4ºC) ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for staining of human tumor cells included: anti-human EpCAM-FITC (BioLegend 324204, clone 9C4, dilution 1:30), anti-human/mouse CD49f-APC (BioLegend 313616 ...
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bioRxiv - Immunology 2022Quote: ... Samples were centrifuged at 400g for 3 minutes at room temperature and resuspended 50μL in PE-Annexin V antibody diluted 1:20 with Annexin V Binding Buffer (BioLegend, 422201), or 50uL Annexin V Binding Buffer only for controls ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were washed once with ice-cold FACS buffer and incubated with 1 μg/mL PE anti-human IgG Fc (Biolegend) at 4 °C with gentle shaking in the dark for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed once with cold PBS and then stained with 100 µL Zombie Aqua (BioLegend; 1:1000 in PBS) for 30 min at RT ...
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bioRxiv - Immunology 2022Quote: ... samples were stained with the following antibodies in 1X eBiosciences permeabilization buffer for 30 minutes at room temperature: T-bet (1:100; clone 4B10; BioLegend); Foxp3 (1:300 ...
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bioRxiv - Cell Biology 2022Quote: One million fixed BM and spleen cells were immunolabelled for one hour at room temperature with Ter119-PE (1:200, Biolegend) and 1ug/ml Hoechst 33342 (Thermofisher ...
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bioRxiv - Neuroscience 2021Quote: ... followed by Biotin-SP-conjugated AffiniPure Goat Anti-Rabbit IgG at a dilution of 1:200 for 30 min at room temperature and incubation with horseradish peroxidase streptavidin at a dilution of 1:125 (405210, BioLegend) for 30 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.4)-fixed coronal brain sections were blocked with 10% donkey serum (Jackson Immunoresearch)/PBS plus 0.3% Triton X-100 in PBS and incubated with antibodies to p-S129-α-synuclein (1:1000; Biolegend), and TH (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated with 1.5% normal serum followed by incubation with the primary antibody (biotinylated anti-Aβ – 1:100, BioLegend, USA) for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: After 7 days of shRNA-mediated knockdown 1 x 105 cells were wash once in PBS and resuspend in Annexin-V Binding Buffer (422201, BioLegend) with 3 μl of Annexin-V-APC (640941 ...
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bioRxiv - Immunology 2020Quote: ... Antibody dilutions in cell staining buffer containing 1 % TruStain fcX™ (anti-mouse CD16/32) Antibody (both BioLegend, London, UK) were used to stain according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Populations of interest based on DAPI negativity expression and lack of lineage markers (APC-Cy7; CD31, Ter119, CD45 at 1 μL/106 cells; Biolegend) expression were directly sorted into DMEM/F12 containing 10% FBS with 1% P/S/A ...
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bioRxiv - Cell Biology 2020Quote: ... washed with 0.5 % BSA/PBS and incubated with 200 µL of a 1:50 dilution of hCD4-BV711 (OKT4, BioLegend, UK) for 30 min ...