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Citations for BioLegend :
7251 - 7300 of 9316 citations for 8 11 Methano 10a 3 6a 1 propanyl 3 ylidene 8H indeno 2 1 b azocine 12 14 dione 5 acetyloxy 4 benzoyloxy dodecahydro 1 3 dimethyl 9 methylene 3R 4S 5R 6aR 6bR 8S 10aR 11R 11aS 15S 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Samples were then washed twice with 1 mL of Intracellular Staining Perm Wash buffer (Cat: 421002, BioLegend, San Diego, CA, USA); after the second wash ...
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bioRxiv - Cell Biology 2021Quote: ... The fixed cells are permeabilized with 0.1% Triton-X 100 for 10min at RT and stained with anti-vimentin (1:200, Biolegend, CA, USA) for 30 min in PBS containing 5% normal goat serum (RT) ...
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bioRxiv - Cancer Biology 2021Quote: Wildtype RMC219 and RMC2C cells were harvested and 1 x 106 cells were resuspended in buffer A (PBS 1X, EDTA 2mM, inactivated FCS 1%) and 5uL of Human TruStain FcX (Biolegend, 422301) was added for 10 min at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... Antigen-independent activation-marker expression of CAR-T cells was evaluated by antibody staining for CD137 (PE/Cy7, clone 4B4-1, BioLegend #309818) and CTLA-4 (PE/Cy7 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell sorting was repeated on cultured CAFs and PVL cells using the previously described experimental conditions with anti-PDGFRα (1:100; BioLegend #323507), anti-CD146 (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... mouse monoclonal anti-DnaK 4RA2 at 1□:□10,□000 dilution (Enzo, ADI-SPA-880) and HRP Goat anti-mouse IgG (BioLegend, 405306) at 1□:□10,□000 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... PBMCs were collected and stained using APC/Fire anti-CD4 and APC anti-CD8 (Supplemental Table 1) (Biolegend, San Diego CA). PBMCs were first washed and stained with Zombie Yellow (Supplemental Table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... CD59 (PE, 143103), PD-1 (BV605, 135220), CD44 (BV711, 103057), CD69 (PE-Cy7, 104512), IFN-γ (APC, 505810) (all from Biolegend). The Foxp3 Fix/Perm Kit was used for intracellular staining (421403 ...
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bioRxiv - Microbiology 2021Quote: We performed western blotting as in (Polino et al., 2020) with primary antibodies mouse anti-HA diluted 1:1000 (clone 16B12; Biolegend #901501), rabbit anti-PfAldolase diluted 1:2000 (abcam #ab207494 ...
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bioRxiv - Systems Biology 2021Quote: Single nuclei from each PDX condition were labeled with 1 μl condition-specific hashtag oligonucleotide-labeled antibodies (BioLegend, TotalSeq A0541-A0545) according to manufacturer’s protocol prior to pooling and loading on a single lane of the 10X Chromium v2 system ...
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bioRxiv - Microbiology 2019Quote: ... Cells were harvested using Trypsin-EDTA and resuspended in FACS buffer (PBS containing 10% FBS and 2mM EDTA) and stained with anti-CD300lf PE clone Tx70 (1:100; BioLegend, #132704) or anti-CD300ld PE clone Tx69 (1:100 ...
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bioRxiv - Cell Biology 2021Quote: ... non-adherent cells were moved to bacteriological Petri dishes the next day and differentiated over 1 week in cDMEM containing 20 ng/mL recombinant murine M-CSF (Biolegend, 576404). Generation of murine bone-marrow derived dendritic cells (BMDC ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was removed and approximately 106 cells were resuspended in FACS buffer containing an anti-HA AF488 conjugate (1:1000, BioLegend, 901509) and an eFluor 780 fixable viability dye (Thermo Fischer Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed once in Cyto-Fast Wash Buffer and incubated in Cyto-Fast Wash Buffer containing an anti-HA AF647 conjugate (1:1000, BioLegend, 682404) and an anti-FLAG AF549 conjugate (for cells expressing FLAG-tagged M ...
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bioRxiv - Molecular Biology 2022Quote: ... Whole-lung suspension was then repelleted and resuspended in SB + 1:50 TruStain FcX™ antibody (anti-mouse CD16/32, BioLegend) for a 10-minute blocking period at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for removal of mouse stromal cells included: anti-mouse H-2Kd-biotin (BioLegend, 116604, clone SF1.1, dilution 1:20) and anti-mouse Cd45-PE/Cyanine5 (BioLegend 103110 ...
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bioRxiv - Cancer Biology 2022Quote: The following primary antibodies with indicated dilution in blocking buffer were used: Rabbit anti-Tuj1 (Covance, BioLegend, MRB-435P, 1:1,000), Mouse anti-Tuj1 (Covance ...
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bioRxiv - Neuroscience 2020Quote: ... The following antibodies were then added to cell suspensions at 1:200 concentrations: CD11b-PE (BioLegend Cat#: 101208; Clone: M1/70), CD45-APC/Cy7 (BioLegend Cat# ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed three times with PBS-T (PBS with 0.1% Tween-20) and 50μl of mouse IgG-specific secondary antibody (BioLegend #405306, 1:10,000) diluted in dilution solution added to each well ...
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bioRxiv - Developmental Biology 2021Quote: ... The following antibodies were added at the concentration of 1:50 and incubated on ice for 45 minutes: APC/Cy7 Cd45 (Biolegend #103116), APC Pecam1 (Biolegend #102410) ...
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bioRxiv - Cell Biology 2021Quote: ... the cell pellets washed twice with 10% FCS in PBS (100μl/well) and then resuspended in 10% FCS in PBS (100μl/well) containing APC/Cyanine7 Goat anti-mouse IgG (Biolegend; 1:250) and incubated on ice for 30mins ...
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bioRxiv - Immunology 2022Quote: Cryopreserved PBMCs were thawed and washed twice with 10 ml of FACS buffer (1 x PBS containing 2% FBS and 1 mM EDTA) and resuspended in 100 μl of 1x PBS containing Zombie UV live/dead dye at 1:200 dilution (BioLegend, 423108) and incubate at room temperature for 15 minutes ...
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bioRxiv - Immunology 2022Quote: ... with the following modifications: Approximately 1 million cells per sample were resuspended in 95 ul of cell staining buffer (Biolegend, USA) with 5 ul of Fc receptor Block (TrueStain FcX ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were then treated with 25 μl of APC-antiHA antibody with 1:75 dilution (~0.07 μg/well, BioLegend catalog # 901523) for 1 hour on ice in the dark ...
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bioRxiv - Biophysics 2019Quote: ... siRNA mediated knockdown was further confirmed by imaging cells labelled with Alexa Fluor 488 anti-human FcƐRIɑ antibody AER-37 (CRA-1) (BioLegend Products, Singapore).
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bioRxiv - Cancer Biology 2019Quote: ... Purity of isolated cells was validated by FACS by using the cell surface markers EpCAM-APC (Biolegend, 118213, 1:50 dilution) and CD45-PE (Biolegend ...
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bioRxiv - Cell Biology 2019Quote: ... blocked with 1 mg/mL human IgG (Privigen, Behring CSL) and stained with mAb 7–239 α-Siglec-1-PE or matched isotype-PE control (Biolegend) at 4°C for 30 min ...
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bioRxiv - Genomics 2019Quote: ... The following antibodies were added (1:100) to each sample and incubated for 30 minutes at room temperature: Alexa Fluor 700 anti-mouse CD8a (Biolegend, 100730), PE/Dazzle-594 anti-mouse CD4 (Biolegend ...
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bioRxiv - Neuroscience 2021Quote: ... All slides were processed at the same time using the purified mouse monoclonal anti-beta-amyloid 1-16 antibody (6E10) (Biolegend, 803001) at 1/500 for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... TnT cells and Jurkat-derived cell lines were stimulated overnight with either 10 μg ml−1 plate-bound anti-human CD3e antibody (clone OKT3, BioLegend, #317326) or 1X eBioscience Cell Stimulation Cocktail (81 nM PMA ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed again and then incubated with horse radish peroxidase (HRP) conjugated anti-mouse IgG secondary antibody (Biolegend; 1:3000) for 1 hour at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... and IL-10 were analyzed using a human COVID-19 Cytokine Storm Panel 1 (13-plex) kit (Cat. No. 741091; BioLegend, USA) according to the manufacturer’s instructions.
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Immunoresolvents Support Skeletal Myofiber Regeneration via Actions on Myeloid and Muscle Stem CellsbioRxiv - Immunology 2020Quote: ... Cells were resupsended and incubated for 30 min on ice with primary antibodies including Sca-1-APC (Biolegend [Clone D7; 108112]), CD45-AF488 (Biolegend [Clone 30-F11 ...
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bioRxiv - Immunology 2021Quote: ... PD-L1 expression in primary tumor-associated LECs was determined using CD45-PE/Cy7 (clone 30-F11, Biolegend 103114, 1:200), CD31-PerCp/Cy5.5 (clone MEC13.3 ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed three times with PBS-T (PBS with 0.1% Tween-20) and 50μl of mouse IgG-specific secondary antibody (BioLegend #405306, 1:10,000) diluted in dilution solution added to each well ...
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bioRxiv - Immunology 2021Quote: ... and a cocktail of fluorescent antibodies: 1 µL each of anti-human CD3 (Brilliant Violet 421-conjugated, Biolegend 344834, clone SK7), anti-human CD69 (PerCP-eFluor710-conjugated ...
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bioRxiv - Immunology 2021Quote: ... human AB serum and were incubated on ice for 30 minutes with the following antibodies: CD66b-FITC (1:100, Biolegend 305104), EpCAM-PE (1:100 ...
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bioRxiv - Cancer Biology 2022Quote: ... The washed samples were stained for 20 min on ice with Brilliant Violet 510™ anti-mouse CD45 Antibody (1:200, Clone: 30-F11, BioLegend), PE/Cyanine7 anti-mouse/human CD11b Antibody (1:200 ...
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bioRxiv - Bioengineering 2022Quote: ... CMs in the ORR field of view were uniquely identified by staining with CM surface maker SIRPa (1:100, Biolegend, 323802). We performed control studies to ensure that the SIRPa signal did not interfere with ORR measurement (Supplementary Fig ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with PBS supplemented with 2% FBS (PBS+) and then stained with 50µl of an antibody cocktail containing 1:50 diluted anti-human CD45-AF700 (BioLegend, clone HI30), 1:50 diluted anti-human CD3-BV605 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... Blocking and antibody solutions were further supplemented with 1:150 Human TruStain FcX™ Fc Receptor Blocking Solution (BioLegend, London, UK), to reduce non-specific binding ...
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bioRxiv - Immunology 2022Quote: ... and incubated with the following antibodies in cRPMI at 1:200 for 30 minutes: CD4-BV650 (#100469; Biolegend, San Diego, USA), CD8-BV605 (#100744 ...
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bioRxiv - Immunology 2022Quote: ... The cells were resuspended in 1 ml FACS buffer and stained with Zombie Near-IR Live/Dead (Biolegend, Cat. No. 423105), CD11b-Pacific Blue (Biolegend ...
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bioRxiv - Synthetic Biology 2022Quote: ... The blocking buffer was subsequently removed and cells were incubated for an additional hour with 1:100 diluted primary antibody (αHA antibody, Biolegend, #901502) in antibody dilution buffer (1x PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... the NHE1 CRISPR silenced organoids and Lgr5DTR-GFP organoids were first dissociated and then stained for CD44 (BioLegend, 103030, 1:250), CD24 (BD ...
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bioRxiv - Developmental Biology 2024Quote: Single-cell suspensions were obtained at day 2.5 of differentiation Cells were first labeled with Zombie NIR fixable viability dye (BioLegend, 1:500) for the exclusion of dead cells ...
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bioRxiv - Immunology 2024Quote: Cells were stained with 20 µL of surface antibody cocktail with Zombie NIR fixable viability kit (1:400-1000, Biolegend, 423105) in PBS for 20 minutes on ice ...
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bioRxiv - Cell Biology 2024Quote: ... all input solutions were supplemented with 1 μg/mL of PE-conjugated rat anti-mouse TNFα antibody (MP6-XT22, BioLegend, UK).
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bioRxiv - Immunology 2024Quote: ... in FACS buffer and stained with anti-hCD19-PE-Cy7 and anti-hCD138-PE-Cy7 (Biolegend, #302216 and #356513, 1:100). After washing ...
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bioRxiv - Immunology 2024Quote: ... Cells were next stained for 30 mins on ice with a 1:300 dilution of antibodies raised against mouse CD206 (Biolegend,141710), CD301(Biorad ...