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Citations for BioLegend :
651 - 700 of 7287 citations for 7H Pyrazolo 4 3 d pyrimidin 7 one 1 4 dihydro 3 b D ribofuranosyl oxime monohydrochloride 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used in this study: Alexa Fluor 488 anti-human SSEA-4 (#330412, clone MC-813-70, Mouse IgG3 κ, dilution 1:100, Biolegend); Alexa Fluor 647 anti-human SSEA-4 Antibody (#330408 ...
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bioRxiv - Immunology 2022Quote: ... Immunostaining was performed by incubating the sections overnight at 4°C with the SMI-32 primary antibody (Biolegend: Suppl. Table 1) in incubation buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... for 10 min at 4°C followed by an incubation with CD45-BV421 (Biolegend 103133, clone 30-F11, 1:200 dilution) and Ter119-BV421 (Biolegend 116233 ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue sections were stained with monoclonal mouse anti-βIII-tubulin primary antibody (Biolegend, Cat. 801202; 1:100 overnight at 4 °C) and goat anti-mouse AF647 (abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated with their respective primary antibodies diluted in 5% skim milk in PBST at 4 °C overnight: purified mouse anti-βIII-tubulin (1:1,000) (BioLegend, cat. 801202), monoclonal mouse anti-GAPDH (1:50,000 ...
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bioRxiv - Immunology 2023Quote: ... surface markers were stained with following antibodies at 1:200 dilution at 4°C for 30 min: CD11b- Pacific Blue (BioLegend, 101224), CD45-BV650 (BioLegend ...
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bioRxiv - Cell Biology 2022Quote: ... The digested tissue was resuspended in 100 μL of 4% FBS in PBS containing an FITC antibody to CD11b (1:100; BioLegend, 101206) and PE antibody to CD45(1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... cell suspensions were stained for 30 min at 4°C in the dark with CD45-BV605 (1:50, BioLegend #304042, RRID:AB_2562106), ITGA6-PeCy7 (1:100 ...
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bioRxiv - Immunology 2022Quote: ... 4°C) and preincubated for 15min with anti-CD16/CD32 Fc-block antibody (10μg/ml; BioLegend, San Diego, CA, 1:50) in FACS buffer followed by a 30min incubation with fluorescence dye-conjugated ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells transfected with pMAX-GFP and either pcDNA3.1-Rai or empty vector were added in a 10:1 ratio and incubated for 4 h in presence of APC anti-LAMP1 antibody (clone H4A3, Biolegend #328620). Monensin (2mM ...
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bioRxiv - Neuroscience 2023Quote: ... After incubation, cells were centrifuged (350rcf, 7min, 4°C) and resuspended in permeabilization buffer containing Streptavidin (PerCP, Biolegend #405213, 1:600) and antibodies directed against intracellular targets Foxp3 (APC ...
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bioRxiv - Bioengineering 2024Quote: ... were coated with 100 µL of 4 µg/mL polyclonal anti-lysostaphin antibody (AICBIOTECH-PAb102) in 1×ELISA coating buffer (BIOLEGEND-421701). Wells were washed with 0.05% Tween 20 (SIGMA-P9416-50ML ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then washed with suspension buffer (PBS + 0.5% BSA + 2 mM EDTA + 4.5 mg/mL D-glucose) and resuspended in RBC Lysis Buffer (Biolegend 420301) for one minute at room temperature to remove red blood cells ...
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bioRxiv - Genomics 2022Quote: ... anti-human CD82-PE/Cyanine 7 monoclonal antibody (BioLegend, #342109, 1:500 dilution) and anti-human CD56-PE/Cyanine 7 monoclonal antibody (eBioscience ...
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bioRxiv - Cancer Biology 2022Quote: ... PE/Cyanine 7 anti-mouse CD31antibody (Biolegend, 102418, clone:390, 1:100 dilution), and PE/Cyanine 7 anti- mouse CD45 antibody (Biolegend ...
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bioRxiv - Physiology 2023Quote: ... Cell suspensions were additionally incubated with 7-AAD Solution (1:100, BioLegend, 420404) for 5 minutes to detect dead cells and passed through cell strainer tubes (Falcon ...
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bioRxiv - Immunology 2024Quote: ... and only in the presence of 1 ng/mL IL-7 thereafter (BioLegend). Cultures were analyzed and passaged every 3 days.
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bioRxiv - Developmental Biology 2021Quote: ... then for 1 hour at 4°C in the dark with a 1:1 mix of rat anti-mouse CD41 (labelled with BV421) (BioLegend – 133911; 0.25 µg per million cells) and rat anti-mouse CD31 (labelled with AF647 ...
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bioRxiv - Immunology 2021Quote: ... were independently stained with TotalSeq™-B0303 anti-mouse Hashtag 3 Antibody (Biolegend, cat: 155835) according to the manufacturer’s instructions and with fluorophore-conjugated antibodies for cell sorting (anti-CD45.1-PB ...
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bioRxiv - Immunology 2019Quote: ... anti-mouse CD86 (GL1) and anti-mouse CD40 (Clone 3/23) were all from Biolegend.
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bioRxiv - Cancer Biology 2019Quote: ... filtered and resuspended in MEMα medium or FACS buffer containing 3 μg/ml DAPI (BioLegend). Cells were sorted on BD FACSAria1 or FACSAria2 machines and collected in 150 μl Arcturus PicoPure Extraction Buffer ...
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bioRxiv - Immunology 2021Quote: ... 3) mouse anti-human CD196 prelabeled with Alexa Fluor 488 (CCR6 marker; clone G034E3, BioLegend) and 4 ...
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bioRxiv - Immunology 2021Quote: ... αCD366 (Tim-3) BV711(RMT3-23) and αTIGIT PE-Dazzle594 (2G9) were purchased from BioLegend, San Diego ...
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bioRxiv - Immunology 2021Quote: Macrophages (~3 x 106) were stained with anti-F4/80 (rat monoclonal FITC-conjugated, BioLegend) in a 1:200 dilution in FACS buffer for 20 minutes on ice ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated with Brilliant Violet 421 anti-CD56 (clone HCD56, Biolegend, 3 μg/ml), APC anti-NKp30 (clone P30-15 ...
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bioRxiv - Cancer Biology 2020Quote: Samples were stained with 10X Chromium 3’ mRNA capture compatible TotalSeq-A antibodies (Biolegend, USA). Staining was performed as previously described by Stoeckius et ...
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bioRxiv - Microbiology 2023Quote: ... lysed in 1X red blood cell lysis buffer for 3 minutes (ref no. 420301, BioLegend), and passed successively through 70μm and 30μm strainers (Miltenyi Biotec) ...
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bioRxiv - Pathology 2023Quote: ... and resuspended in 3 ml red blood cell lysis buffer (Biolegend, San Diego, CA, USA) for 90 s to remove the remaining red blood cells and diluted in 9 mL PBS after incubation ...
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bioRxiv - Pathology 2023Quote: ... and suspended in 3 ml red blood cell lysis buffer (Biolegend, San Diego, CA, USA) for 90 s to remove the remaining red blood cells and diluted in 9 mL PBS after incubation ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... Granzyme B (Biolegend, BV421), IL-4 (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: ... granzyme-B (GB11; Biolegend), STAT1 Phospho (Ser727 ...
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Natural killer activation for bladder cancer elimination can be achieved in vitro by heat-killed BCGbioRxiv - Immunology 2020Quote: ... Granzyme B-PE (Biolegend) for 30 min ...
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bioRxiv - Immunology 2020Quote: ... and granzyme B (BioLegend). Granzyme B was activated using Cathepsin C/DPPI (R&D Systems ...
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bioRxiv - Cancer Biology 2023Quote: ... Granzyme B (Biolegend, 515408), IFNγ (Biolegend ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were applied overnight at 4°C and included SMI31 (Biolegend, 801601), IBA1 (Wako ...
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bioRxiv - Neuroscience 2019Quote: ... were incubated with a combination of 4 µg Tau12 (Biolegend, cat no 8065), 4 µg Tau 5 (Abcam ...
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bioRxiv - Immunology 2019Quote: Neutrophils obtained after 4-day differentiation were washed and labeled using CFSE (BioLegend). Delta T dishes (Bioptech ...
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bioRxiv - Immunology 2021Quote: ... MDM were suspended in 100μL of 4°C annexin V binding buffer (BioLegend) and the two pHrodo loaded sets were stained with 2μL of FITC Annexin V (BioLegend) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then fixed in 4% paraformaldehyde and permeabilized using Permeabilization buffer (Biolegend). Intracellular proteins were stained with antibodies against TNF (MP6-XT22 ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were incubated overnight at 4°C in primary antibody mixtures (HIF1α, Biolegend; EZH2 ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubation with Human TruStain FxC (10 minutes at 4°C; Biolegend, #422302) to block Fc receptor-mediated binding ...
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bioRxiv - Immunology 2020Quote: ... and cultured for 4 days +/- αIL10 antibody +/- isotype control (5μg/ml, both Biolegend). Assessment of the direct effect of IL-10 on human CD4 T cells was performed by plate bound αCD3/28 (1μg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... Remaining volume was incubated at 4°C with anti-HA antibody (Biolegend, #901513) at 1:150 dilution for 4hrs on a gentle spinner ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed for 10 min at 4°C using IC fix (Biolegend) and cells were then incubated in for 20 min at RT in Permeabilization buffer (biolegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... we added 4 μg/ml PE-labeled anti-CD63 antibody (BioLegend, clone H5C6). We imaged the nanowell using Zeiss Axio Observer Z1 microscope equipped with 20x/0.8 NA objectives and a Hamamatsu Orca Flash v2 camera.
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bioRxiv - Developmental Biology 2023Quote: ... additional staining with anti-CD34 (4 µL CD34 APC/Cy7, clone: 581, Biolegend) and anti-CD14 antibodies (3 µL CD14 PE-CF594 ...
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bioRxiv - Immunology 2023Quote: ... 4 µL of cocktail containing PMA/ionomycin + Brefeldin A (Biolegend, San Diego, CA) was added ...
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bioRxiv - Immunology 2024Quote: ... or Th2-polarizing condition (10 ng/mL recombinant mouse IL-4 (#574304, BioLegend), and 10 μg/mL anti-mouse IL-12/23p40 antibody (clone C17.8 ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed and stained with CTLA-4-PE-Dazzle 594 (Biolegend), Ki67-PerCP-eFluor 710 (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... at 4°C for 48 hrs: goat anti-mouse Alexa Fluor 488 (Biolegend), goat anti-rabbit Alexa Fluor 594 (Abcam) ...