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Citations for BioLegend :
6601 - 6650 of 7730 citations for Insulin ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... cultures were stained for flow cytometry with anti-CD69-PE/Cy7 (1:150) (BioLegend, Cat.#310912) (Y780/60-A) ...
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bioRxiv - Neuroscience 2023Quote: ... the pellets were resuspended in 90 µL FACS buffer with Fc block (4G2, 1:90, BioLegend) and transferred to a 96 well-plate ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FBS in presence of GM-CSF (20 ng ml−1, BioLegend Cat# 572903) and IL-4 (20 ng ml−1 ...
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bioRxiv - Immunology 2023Quote: ... and purified anti-mouse IgM (RMM-1) antibodies were purchased from BioLegend (San Diego, CA, USA). Purified anti-mouse CD19 (MB19-1) ...
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bioRxiv - Immunology 2023Quote: Cells were harvested and surface stained with the following antibodies: CD57-FITC (HNK-1) from BioLegend; KIR3DL1S1-APC (Z27.3.7) ...
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bioRxiv - Microbiology 2023Quote: ... Alexa Fluor 488 goat anti-mouse TER-119 (cat. no. 116215, BioLegend; IFA, 1:500 dilution), Alexa Fluor 488 conjugated streptavidin (cat ...
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bioRxiv - Immunology 2023Quote: Extracellular staining was performed using the following antibodies: anti-human CD3ε BV785 (1:50, OKT3, BioLegend), anti-human CD19 BV605 (1:20 ...
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bioRxiv - Immunology 2023Quote: ... cells were instead stained with a 1:1000 dilution of Zombie NIR Viability Dye (Biolegend, #423105) in PBS on ice for 10 minutes prior to surface antibody staining.
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-human CD274 (B7-H1, PD-L1) (BioLegend, cat. no. 329707; 1:1000 for WB); mouse anti-DAAM1 (Santa Cruz Bio ...
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bioRxiv - Cell Biology 2023Quote: ... before being immunoblotted with mouse anti-GFP (1:3000 dilution, 902601, BioLegend, San Diego, CA, USA) and mouse anti-Cdc28 (1:3000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... a 1:500 dilution of Alexa Fluor™ 700 anti-murine CD24 (BioLegend, Cat. No. 101835), and a 1:500 dilution of Alexa Fluor™ 647 anti-murine Siglec-F (BioLegend ...
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bioRxiv - Cell Biology 2023Quote: ... a 1:400 dilution of APC/Fire™ 750 anti-murine CD11c (BioLegend, Cat. No. 117351), a 1:400 dilution of PE-Cyanine7 anti-murine CD144 (BioLegend ...
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bioRxiv - Cell Biology 2023Quote: ... a 1:400 dilution of Brilliant Violet™ 510 anti-murine Ly6C (BioLegend, Cat. No. 128033), a 1:400 dilution of Brilliant Violet™ 605 anti-murine MHC-II (BioLegend ...
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bioRxiv - Cell Biology 2023Quote: ... a 1:400 dilution of Brilliant Violet™ 605 anti-murine CD4 (BioLegend, Cat. No. 100548), a 1:400 dilution of PerCP-Cyanine5.5 anti-murine CD3 (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... and then stained with 1:66 PE-Cy7 anti-human CD71 (TFRC) (BioLegend; Cat. No. 334112) for 30 minutes in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... a 1:400 dilution of APC/Fire™ 750 anti-murine CD115 (BioLegend, Cat. No. 135536), a 1:400 dilution of Brilliant Violet™ 421 anti-murine CD11b (BioLegend ...
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bioRxiv - Cell Biology 2023Quote: ... a 1:400 dilution of Brilliant Violet™ 421 anti-murine CD11b (BioLegend, Cat. No. 101251), a 1:400 dilution of Brilliant Violet™ 510 anti-murine Ly6C (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µl of TotalSeqB Hashtag (anti-Nuclear Pore Complex Proteins Hashtag at 0.5g, Biolegend 682239-682243) was added to each sample for subsequent identification in downstream analysis ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were incubated with Human TruStain FcXTM (FC Receptor Blocking Solution, 1:250, BioLegend catalog #422302) for 5 minutes at room temperature at a 1:250 dilution in DPBS with 1% BSA ...
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bioRxiv - Cell Biology 2024Quote: ... resuspended in 100µL DPBS and incubated with antibodies (1/100, all from BioLegend®, otherwise indicated) directed against human CD19 (HIB19) ...
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bioRxiv - Molecular Biology 2024Quote: ... and myeloid cells were stained with Brilliant Violet 421-conjugated anti-CD11b antibody (1:100; Biolegend) for 1 hour in the dark with gentle agitation ...
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bioRxiv - Immunology 2024Quote: ... Supplementary Table 1) and 1ul/staining volume of hashtag TotalSeqC antibody mastermix (Biolegend, Supplementary Table 2) was added to each sample and cells were incubated for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... live CD45+ cells were stained with the anti-mouse lineage negative cocktail (1:200; 133303 Biolegend), CD25 (1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... anti-hyperphosphorylated neurofilament heavy chain (p-NF, mouse monoclonal, clone SMI 31P, from Biolegend, 1:100), Purified anti-Neurofilament H (NF-H) ...
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bioRxiv - Cancer Biology 2024Quote: ... incubated in FC-blocking mix (PBS containing human anti-CD16/CD32/CD64, 1:100 dilution, BioLegend) for 10 min at 4 °C and subsequently stained for surface markers in antibody mix in PBS for 30 min at 4 °C in the dark ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies were used for staining: PerCP anti-mouse CD45 (Biolegend, 30-F11, 1:100) or BUV496 anti-mouse CD45 (BD ...
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bioRxiv - Bioengineering 2024Quote: Hydrogel full antibody stain included anti-I-A/I-E (1:800 dilution; FITC; BioLegend, 107605), anti-CD45 (1:800 ...
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bioRxiv - Immunology 2024Quote: ... for 30 mins and permeabilized with 1 × Intracellular Staining Permeabilization Wash Buffer (Cat No: 421002 Biolegend). The optimized concentrations of directly conjugated primary antibodies for intracellular antigen detection were added to samples and incubated for 30 minutes at 4 °C in the dark ...
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bioRxiv - Immunology 2024Quote: ... each knock-out was stained with a unique TotalSeqTM-A hashtag oligo (HTO) 1-10 (BioLegend) combination ...
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bioRxiv - Immunology 2024Quote: ... or FITC-labeled mouse anti human CD14 fluorescent antibody (cat. no. 301804; 1:1,000; BioLegend, Inc.) was added into each tube ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated in a primary antibody cocktail containing anti-SMI312 (Biolegend, 837904, 1:500 dilution), anti-MAP2 (Cell Signaling Technology ...
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bioRxiv - Immunology 2024Quote: ... Single-cell suspensions were then incubated with anti-mouse CD16/32 (1:100; clone 93; BioLegend) diluted in 2 mM EDTA/PBS to block Fc receptors at 4°C for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... 1 mM EDTA and FC block (Purified anti-mouse CD16/32, clone 93, BioLegend, Cat Nr101302) at 1:200 dilution at room temperature ...
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bioRxiv - Immunology 2021Quote: ... were determined using bead based immunoassays (CBA kit, Cat. 560484, BD PharMingen, San Diego, CA, USA; and LEGENDplex, Cat. 740003, BioLegend, San Diego, CA, USA). Log-transformed data were used to construct standard curves fitted to 10 discrete points using a 4-parameter logistic model ...
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bioRxiv - Cancer Biology 2021Quote: ... Further preparation for FACS measurement was following the protocol of the BioLegend APC Annexin V Apoptosis Detection Kit and DNA-staining with DAPI Reagent (25 μg/mL) (Biolegend, San Diego, CA, USA). 20 000 cells were assayed using a flow cytometer FACSCantoII (Becton Dickinson ...
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bioRxiv - Immunology 2021Quote: ... Naïve CD8+ T cells were then negatively-selected (CD8+CD44- purity > 90%) from the resulting single-cell splenocyte suspension using the MojoSort™ Mouse CD8 Naïve T Cell Isolation Kit (BioLegend, San Diego, CA, USA). Two modifications were introduced relative to the manufacturer’s guidelines to increase purity ...
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bioRxiv - Immunology 2024Quote: ... Single cell suspensions were labelled with antibodies against cell surface antigens as described above and then prepared using a Cyto-Fast Fix/Perm kit (BioLegend, San Diego, CA, US), according to the manufacturer’s instructions ...
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The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... Individual cytokine detection kits were also used to quantify mouse IL-6 and TNF-α (Bio-techne, France) and GM-CSF (Biolegend, San Diego, USA).
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bioRxiv - Bioengineering 2022Quote: ... cells were washed with DPBS and harvested with trypsin-EDTA and the viability analyzed by flow cytometry using the Apoptosis Detection Kit with propidium iodide (PI, BioLegend, San Diego, CA, USA) in a Cytoflex flow cytometer (Beckman Coulter ...
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bioRxiv - Cancer Biology 2023Quote: Flow cytometry viability staining was conducted by suspending murine spleen and tumor single cell suspensions in Zombie Violet fixable viability kit (Cat # 423114, BioLegend, San Diego, CA, USA) according to manufacturer instructions for 30 minutes at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... Transduced T cells were positively selected with a PE selection kit (EasySep, ref: 17684) and an anti-HA PE-conjugated antibody (Biolegend, ref: 901518, clone: 16B12) at a 1:50 dilution to enrich transduced T cells ...
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bioRxiv - Immunology 2021Quote: ... 1-5 * 106 cells were stained with antibodies specific for surface markers Thy1.2 (clone 30-H12, BioLegend), Thy1.1 (clone OX-7 ...
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bioRxiv - Developmental Biology 2020Quote: ... Juvenile islet cells from SIX3-Flag pseudoislets were stained with anti-FLAG antibody-555 (1:100; Biolegend). Labeled cells were sorted on a special order 5-laser FACS Aria II (BD Biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... mouse monoclonal anti-nonphosphorylated neurofilament H (clone SMI 32, Cat. # 801701, 1:1,000, BioLegend, San Diego, CA), and goat anti-choline acetyltransferase antibody (Cat ...
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bioRxiv - Molecular Biology 2020Quote: ... The specific antibodies used were anti-CD63 conjugated with FITC (CD63-FITC; 1:150, Cat. #: 353006, Biolegend); CD9-PE (1:1500 ...
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bioRxiv - Immunology 2022Quote: ... media was supplemented with 2 μg/ml of mouse IgG1 K anti-human PD-1 antibody (Biolegend) either alone or in combination with 1 μg/ml mouse IgG2 B anti-human TIGIT (R&D systems ...
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bioRxiv - Immunology 2021Quote: ... Alexa Fluor® 700 anti-human CD4 (clone RPA-T4; dilution 1:200; Biolegend; Cat no 344808), FITC anti-human CD8a (clone RPA-T8 ...
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bioRxiv - Cancer Biology 2019Quote: ... The cells were stained labeled with CD45 PE (Leukocytes) to minimize immune contamination (Biolegend #BL304008, 1:200), EPCAM/CD326 APC (Biolegend #324208 ...
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bioRxiv - Microbiology 2019Quote: ... Sputum samples were also stained with 1 µg/ml of PE anti-human CD45RO in PBS (BioLegend) for 30 min to stain activated and memory T cells ...
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bioRxiv - Physiology 2019Quote: ... and stained with biotinylated antibodies against CD11b to identify myeloid cells (1:1000, catalog number 101204, BioLegend), CD3 to identify T cells (1:250 ...