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Citations for BioLegend :
6401 - 6450 of 7730 citations for Insulin ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The antibodies used for IFA were: mouse anti-HA antibody (clone 16B12, BioLegend; 1:200), rabbit polyclonal anti-HA SG77 (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... BMMs were first stained with fluorochrome-conjugated anti–CD11b (1:200 dilution, BioLegend, no. 101208), and anti–Ly6C (1:200 dilution ...
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bioRxiv - Immunology 2023Quote: ... the BMMs were stained with fluorochrome-conjugated anti– CD11b (1:200 dilution, BioLegend, no. 101226), anti–Ly6C (1:200 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... in the presence of Human TruStain FcX™ Fc Receptor Blocking Solution (1:400, BioLegend), and fixed with 0.5 mL methanol-free paraformaldehyde 4% (Thermo Scientific™ Pierce™ ...
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bioRxiv - Pathology 2024Quote: ... and T-cells were visualized by staining with anti-mouse Ly6G (Biolegend, dilution 1:100), anti-mouse CD68 (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... Single cell suspensions were then stained with 1 ul Zombie live/dead staining dye (Biolegend) in 500ul pf PBS at 4°C for 15 min ...
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bioRxiv - Genetics 2023Quote: ... and pSTAT3 AlexFlour488 (2.5 µL per 100 µL stain, Biolegend, cat#651006, clone 13A3-1) for 30 minutes prior to acquisition on a BD 5-laser Fortessa flow cytometer.
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bioRxiv - Cancer Biology 2023Quote: ... Samples were stained with the following antibodies: anti-CD38-BV421 (1:20, Biolegend, clone HIT2), anti-CD10-BV605 (1:40 ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were stained with the following antibodies: anti-CD38-BV421 (1:20, Biolegend, clone HIT2), anti-CD10-BV605 (1:40 ...
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bioRxiv - Cancer Biology 2023Quote: Cells were stained with the following antibodies: anti-CD34-PE (1:160, Biolegend, clone 581), anti-CD3-PE/Cy7 (1:100 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were stained for CD69 (clone FN50, BioLegend, dilution 1:500 in PBS + 2% FBS) expression after 4 - 24 hr at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse monoclonal anti α2s-spectrin (clone D8B7, 1 :100 for IF) was from Biolegend (#803201). Rabbit polyclonal anti 480-kDa ankyrin G (residues 2735–2935 of rat 480-kDa ankyrin G ...
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bioRxiv - Bioengineering 2023Quote: ... 1.5×106 cells were stained in PBS with 1:200 CD16/CD32 Fc Block (Biolegend) and 1:500 Live/Dead fixable dye (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... a 1:400 dilution of PE-Cyanine7 anti-murine F4/80 (BioLegend, Cat. No. 139314), a 1:500 dilution of Alexa Fluor™ 700 anti-murine CD86 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... Donor 1 PBMCs were stained with a cocktail of anti-human CD3 PerCP-Cy5.5 (Biolegend), CD8 PerCP-Cy5.5 (Biolegend) ...
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bioRxiv - Immunology 2024Quote: Cells were stained with 1:2000 in Zombie red fixable viability dye (Biolegend, Cat#423109) 20 min on ice ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-HA clone 16B12 (1/3000 dilution for HEK-293 cell lysates, Biolegend # 901513), rabbit anti-HA clone C29F4 (1/1000 dilution for brain membrane protein preparations ...
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bioRxiv - Immunology 2023Quote: ... and different TotalSeq-C Anti-Mouse Hashtags (TotalSeq-C Anti-Mouse Hashtags 1-10 Biolegend #155861-155879 (Supplemental Tables 1 and 2) ...
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bioRxiv - Cell Biology 2024Quote: Antibodies and staining reagents used in this study: CD45-BV421 (30-F11, Biolegend, 1:100), Lrig1 (R&D Systems AF3688 ...
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bioRxiv - Cell Biology 2024Quote: ... Then cells were then incubated with primary antibody against vimentin (Chicken, Biolegend #919101, 1:2000) for 45 minutes at room temperature after blocking with 2.5% BSA ...
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bioRxiv - Cell Biology 2024Quote: ... cells were stained with cell surface marker antibodies (CD38-V450 Clone: HIT2 (Biolegend, 1:100), CD45RA-PE-Cy7 ...
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bioRxiv - Cancer Biology 2024Quote: ... The following antibodies were used: anti-CD45-BV605 (BioLegend, 103139, clone 30-F11, 1:100), anti-CD11b-PE-Cy7 (Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... Dissociated cells were stained with Allophycocyanin (APC)-conjugated antibodies against CD133 (1:400; 141207; BioLegend). Cells were sorted on a FACS Aria fusion cell sorter (Becton Dickinson ...
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bioRxiv - Neuroscience 2024Quote: ... Immunodetection was achieved with the primary antibodies (1:1000 in blocking solution): anti-HA (BioLegend), anti-Flag (Sigma-Aldrich) ...
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bioRxiv - Genomics 2024Quote: ... Fc receptor blocking was performed using a 1:50 dilution of Human TruStain FcX (Biolegend) in cell staining buffer for 10 minutes at 4 degrees ...
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bioRxiv - Immunology 2024Quote: ... For immunophenotyping of Tregs cells (1×106/sample) splenocytes were stained with CD4 (PerCP; BioLegend), CD25 (APC ...
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bioRxiv - Immunology 2024Quote: ... Brilliant Violet 711™ anti-mouse/human CD11b antibody (1:200; Biolegend, Clone M1/70), and FITC anti-mouse/human CD45R/B220 antibody (1:200 ...
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bioRxiv - Immunology 2024Quote: ... Antibodies used for flow cytometry included: anti-mouse Ly108 (330-AJ, Biolegend, 134608, 1:200), anti-mouse Tim3 (RMT3-23 ...
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bioRxiv - Immunology 2024Quote: ... Neutrophils and macrophages were stained using Alexa647 conjugated rat anti-Ly6G (Biolegend #127609, 1:100) and Alexa561 conjugated rat anti-F4/80 (Invitrogen #505-4801-80 ...
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bioRxiv - Neuroscience 2024Quote: ... incubated with primary antibody in block (anti-β-amyloid clone 6E10; 1:2,000; BioLegend; 803001) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... TF and ICS was performed following permeabilization using True-Nuclear buffer kit according to the recommended protocol (True-Nuclear Transcription Factor Buffer Set; BioLegend, San Diego, CA). Samples were acquired on a Cytek Aurora (Cytek ...
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bioRxiv - Neuroscience 2019Quote: ... Red blood cells were osmotically lysed and CD4+ T-cells were isolated using a MojoSort Mouse CD4+ T Cell Isolation Kit (Biolegend, San Diego, CA). Purified CD4s were labeled with Cell Proliferation Dye eFluor450 (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... and subjected to the staining of 332 surface antigens using LEGENDScreen Human Cell Screening PE Kit (cat.no. 700001; Biolegend, San Diego, CA, USA). Data acquisition was performed on FACSVerse (Becton Dickinson (BD) ...
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Antigen-dependent inducible T cell reporter system for PET imaging of breast cancer and glioblastomabioRxiv - Synthetic Biology 2022Quote: ... CD4+ T cells and CD8+ T cells were separated using Biolegend MojoSort Human CD4 T Cell Isolation Kit (Biolegend #480130, San Diego, CA) following manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: Apoptotic cells were analyzed by flow cytometry using the FITC Annexin V Apoptosis Detection Kit with propidium iodide (BioLegend, San Diego, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... and proceeded to Annexin V-fluorescein isothiocyanate (FITC)/PI staining according to the manufacturer’s instructions (FITC Annexin V Apoptosis Detection Kit with PI; BioLegend, San Diego, CA, USA). Cells stained with Annexin V (both Annexin V+- and Annexin V+/PI+-cells ...
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bioRxiv - Immunology 2020Quote: ... DCs were isolated from spleens and lymph nodes of C57BL/6 mice using a MojoSort™ Mouse Pan Dendritic Cell Isolation Kit (BioLegend cat. 480097). DCs were cultured with supernatants of necrotic keratinocytes generated by an overnight culture of UV-irradiated (2 J/m2/s for 40 min ...
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bioRxiv - Microbiology 2022Quote: ... The levels of serum cytokines and chemokines were determined by a bead-based multiplex assay using the LEGENDplex mouse anti-virus response panel (13-plex) kit (#740622, Biolegend, San Diego, USA), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: The metabolic activity of HDF cells cultured exposed to the films was analysed using a resazurin assay (Deep Blue Cell Viability Kit (BioLegend, San Diego, USA)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then stained for viability using Zombie NIR™ fixable viability kit for 15 min at room temperature (Cat # BioLegend, CA, USA), followed by fluorescently labeled high- or low-molecular weight HA (2.5mg/ml in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Spleenocytes were resuspended in 3 ml MojoSort™ buffer and enriched for CD8+ T cells using the MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend, 480035), following manufactureŕs instructions.
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bioRxiv - Cell Biology 2024Quote: ... naïve CD4 and CD8 T-cells were isolated from syngeneic cryopreserved PBMC aliquots using the MojoSort magnetic negative selection kits (BioLegend, 480041 and 480045). Naïve T-cells were incubated in complete medium supplemented with 10 IU/mL recombinant human IL-2 (R&D Systems ...
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bioRxiv - Immunology 2024Quote: NK cells were enriched from splenocytes from WT or Pvrig KO C57BL/6 mice by using the MojoSort™ Mouse NK Cell Isolation Kit (Biolegend, cat. 480049). Enriched NK cells were cultured and stimulated in vitro in RPMI-1640 Medium (ATCC ...
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bioRxiv - Immunology 2023Quote: CD3+ T cells were purified from BALB/c mouse spleen cells with the MojoSort mouse CD3 T cell isolation kit (Biolegend, San Diego, CA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: CD8+ cells were isolated from the spleens of four 8-week-old diabetic female NY8.3 NOD mice using a MojoSort Mouse CD8 T-cell isolation kit (Cat# 480043, BioLegend, San Diego, CA, USA) and MojoSort buffer (Cat# 480017 ...
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bioRxiv - Biochemistry 2020Quote: ... HA.11 Epitope Tag Antibody (1:1000, 901513) was from BioLegend (previously Covance catalog# MMS-101R). V5-Tag (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... prior to staining for 1 h at 4°C with the following antibodies from BioLegend (most) or Novus Biologicals (MARCO ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were washed with PBS and stained with fixable Zombie NIR dye (Biolegend, 1:500) for 20 min at RT ...
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bioRxiv - Cell Biology 2020Quote: 1×106 epithelial target cells were washed and re-suspended in the cell staining buffer (Biolegend) containing different dye-conjugated primary antibodies at 1 μg/ml or 5 μg/ml for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... anti-HLA DR (conjugated to Brilliant Violet 421, Biolegend Clone L423 or Abcam ab92511 1:100). Cells were imaged with a widefield Nikon TE200 microscope and images were processed using ImageJ ...