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Citations for BioLegend :
601 - 650 of 7268 citations for Estradiol Serum ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Plates were washed three times and incubated ∼7min with 50 µl of TMB substrate (BioLegend). The reaction was stopped with 25µl of 1M phosphoric acid (Sigma ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were stimulated with plate bound αCD3 and αCD28 (both 1μg/ml, Biolegend). After 12 hours ...
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bioRxiv - Immunology 2021Quote: ... Cells were activated in a 24-well plate coated with anti-CD3 (clon: 500A2, Biolegend) and soluble anti-CD28 (clon ...
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bioRxiv - Immunology 2020Quote: ... tissue-culture plates were coated with 5 μg/mL purified anti-CD3 (clone HIT3a, Biolegend) at 4°C overnight and subsequently washed twice with 1X PBS ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed and 50 μl/well of TMB High Sensitivity substrate solution (BioLegend, UK) was added ...
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bioRxiv - Cancer Biology 2021Quote: ... and cultured in 96-well plates coated with 1µg/ml anti-CD3 (BioLegend, Clone UCHT1) or control mIgG (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were activated on plates coated with anti-CD3 (1mg/ml, Biolegend, #317347, clone: OKT3) and anti-CD28 (1mg/ml ...
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bioRxiv - Immunology 2022Quote: ... The plate was washed five times with PBST and developed with tetramethylbenzidine substrate (Biolegend, 421101). After 20 min at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Plates were washed 3X with PBST before adding HRP goat anti-mouse antibody (BioLegend 405306) at a 1:10,000 dilution in PBST ...
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bioRxiv - Immunology 2023Quote: ... 24-well plates have been coated with 1μg purified human TCRγ/δ antibody (331202, BioLegend) per well overnight at 4 °C ...
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bioRxiv - Immunology 2024Quote: Plates for CD8+ T-cell activation were coated with 5μg/mL α-CD3 (Biolegend, 100239) in phosphate buffered saline (PBS ...
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bioRxiv - Systems Biology 2023Quote: ... Plates were coated with 3.33 μg/mL α-CD3 (HITa3) and α-CD28 antibodies (Biolegend), in 100 μL PBS at 4°C overnight ...
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bioRxiv - Bioengineering 2024Quote: ... plates were coated with 2Lμg/ml of a mouse anti-human CD4 antibody (BioLegend, 300502) diluted in PBS overnight at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... and stimulated with plate bound anti-human CD3 Antibody (clone OKT3, 5ng/mL, #317326, Biolegend) and aCD28 (clone CD28.2 ...
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bioRxiv - Immunology 2021Quote: Naïve CD8+ T cells (OT-1 or Pmel-1) were isolated from pooled spleen and lymph nodes using MojoSort™ mouse CD8 T cell isolation kit (Biolegend) following manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... and then stained at room temperature for 15 min with a 1:200 dilution of the LIVE/DEAD Zombie Aqua™ Fixable Viability Kit (Biolegend) reagent to exclude dead cells ...
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bioRxiv - Immunology 2020Quote: Supernatants of stimulated T cells were harvested on day 6 of culture and analyzed using the LEGENDplex™ human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Bioengineering 2021Quote: ... and IL-10 were analyzed using a human COVID-19 Cytokine Storm Panel 1 (13-plex) kit (Cat. No. 741091; BioLegend, USA) according to the manufacturer’s instructions.
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: ... an aliquot of 3×105 cells in 1ml MACS buffer was incubated at RT for 15 minutes with 1:500 dilution of Zombie UV™ Fixable Viability Kit (Biolegend). Cells were stained with 1 µl of anti-GFP antibody (Santa Cruz ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated in 200 μl FACS buffer containing 1 μl Zombie NIR fixable dye for 30 min to determine cell viability (Zombie Green™ Fixable Viability Kit, BioLegend). Cells were fixed for 15 min in 200 μl PFA 2 % ...
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bioRxiv - Immunology 2024Quote: Cells were stained with 20 µL of surface antibody cocktail with Zombie NIR fixable viability kit (1:400-1000, Biolegend, 423105) in PBS for 20 minutes on ice ...
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bioRxiv - Systems Biology 2019Quote: ... Stained cells were washed with HBH (Hank Balanced Salt Solution (HBSS) with 0.1% bovine serum albumin (BSA) and 10mM HEPES and stained with streptavidin-PerCP/Cy5.5 (BioLegend). Stained cells were washed ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were then washed with HBH (Hank Balanced Salt Solution (HBSS) with 0.1% bovine serum albumin (BSA) and 10mM HEPES) and stained with streptavidin-PerCP/Cy5.5 (BioLegend).
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bioRxiv - Immunology 2020Quote: ... Human trachea was stained for 72 hours in PBS serum and the following antibodies: hCD103-AlexaFluor 594 (Biolegend) 1:40 ...
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bioRxiv - Immunology 2020Quote: ... The cells were stained in FACS buffer (PBS containing 0.5% bovine serum albumin and 0.05% sodium azide) with fluorescently labelled antibodies from BioLegend (CD11c-APC/Cy7 ...
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bioRxiv - Immunology 2024Quote: ... and blocked using 3% FCS or mouse serum in Facs buffer and/or Tru block monocyte blocker (Biolegend) as required ...
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bioRxiv - Cell Biology 2022Quote: ... - β1 (clone HMb1-1, 1:200) (Biolegend), were also employed ...
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bioRxiv - Immunology 2023Quote: ... we employed the LEGENDplex™ Human Inflammation Panel 1 (13-plex) flow cytometry-based assay kit (#740809, BioLegend, San Diego, CA, USA). This panel allowed us for simultaneous quantification of 13 human inflammatory cytokines and chemokines (IL-1β ...
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bioRxiv - Immunology 2023Quote: Serum BAFF quantitation was performed on plasma samples from 12-14 week-old Sh2b3E372K mice using the LEGENDplex Mouse B cell Panel – S/P (1-plex) w/VbP kit (BioLegend; cat # 740983) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... cells were washed twice with PBS and stained using the Zombie NIR Fixable Viability Kit (Biolegend, 423106, diluted 1:500 in PBS) for 30 min at 4°C prior to Fc blocking and staining of surface antigens ...
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bioRxiv - Bioengineering 2024Quote: ... were first stained with Zombie Aqua™ fixable viability kit (1:500 dilution in PBS) and Fc-blocked with TruStain FcXTM (anti-mouse CD16/32) antibody (BioLegend, CA, U.S.A.) (1:20 dilution in PBS (1% BSA) ...
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bioRxiv - Immunology 2022Quote: Serum samples were screened for neutralization using LEGENDplex SARS-CoV-2 neutralizing antibody assay (BioLegend, San Diego, CA, USA) following manufacturer recommendations ...
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bioRxiv - Immunology 2019Quote: The serum immunoglobulin levels were measured with LEGENDplex Mouse Immunoglobulin Isotyping Panel according to the manufacturer’s protocol (Biolegend, 740493).
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bioRxiv - Neuroscience 2022Quote: ... Muscle sections were incubated overnight at 4 °C in blocking solution (2 % BSA, 10 % Normal Goat Serum, 0.1 % Triton and PBS) with chicken anti-NFM (#822701, BioLegend, USA ...
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bioRxiv - Microbiology 2022Quote: Serum cytokine levels were determined using a beads-based immunoassay with a custom-designed mouse cytokine panel (LEGENDplex, BioLegend) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... dilutions of the serum were mixed with a constant 3 nM of an HRP conjugated anti-OVA antibody (BioLegend) and incubated for 2 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: IL-1β release was measured in murine serum using the LEGENDplex™ mouse inflammation panel bead-based immunoassay (BioLegend) and flow cytometry as described above except shaking was performed at 800 rpm.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... They were incubated in high glucose Dulbecco’s’ modified Eagle medium (DMEM) (Capricorn) supplemented with 10% fetal bovine serum (Biolegend), 100 U/mL of penicillin (Lonza ...
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bioRxiv - Immunology 2020Quote: ... followed by 10-minute incubation on ice with blocking buffer containing 10% mouse serum with 0.25 μg/ml anti CD16/CD32 (BioLegend). Cells where incubated with indicated antibodies (Supplementary Table 5 ...
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bioRxiv - Immunology 2020Quote: ... Bacteria were opsonised in heat-inactivated human serum for 30 minutes at 37°C and labelled via incubation with 10μM carboxyfluorescein succinimidyl ester (CFSE) (Biolegend) for 30 minutes at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... kidney epithelial) cells consisted of high glucose Dulbecco’s modified Eagle medium (DMEM) (Capricorn) with 10 % fetal bovine serum (FBS) (Biolegend), 100 U/mL of penicillin (Lonza ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently resuspended in staining buffer (PBS with 0.5% bovine serum albumin and 2 mM EDTA) and Fc-blocked using TruStain FcX reagent (BioLegend). Cells were then stained with the following fluorophore-conjugated anti-mouse antibodies for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... IL-5 and IL-13 were measured in control subject and COPD patients’ serum and SP or EP-stimulated MDMs supernatants using LEGENDplex HU Th Panel according to manufacturer’s instructions (BioLegend). Aquisition was performed on Accuri C6 Flow Cytometer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Th2 cells were cultured for 4 days with plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-4 (4 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... Th17 cells were differentiated in the presence of plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-6 (20 ng/ml ...
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bioRxiv - Immunology 2021Quote: 96-well U-bottomed plates were coated with 100μL of 0.5μg/mL streptavidin (BioLegend Cat#270302) at room temperature (18-25°C ...
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bioRxiv - Immunology 2022Quote: ... coated flat bottomed 96 well plates with soluble anti-CD28 (2 μg/mL) (ULTRA-LEAF Biolegend), and with or without human recombinant soluble BAFF at 500 ng/mL ...
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bioRxiv - Microbiology 2022Quote: ... the plates were incubated with biotinylated detection antibodies against IL-12 (Biolegend Cat#505302, clone C17.8) and IL-1β (Biolegend Cat#515801 ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed 4 times with PBS and 40μl of HRP anti-rabbit IgG antibody (Biolegend) 1:1500 was added to each well and incubated for 2 hours at room temperature ...
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bioRxiv - Immunology 2023Quote: ... 96-well plates were pre-coated with 5 μg/ml anti-hCD3 (BioLegend, Cat# 317326, RRID:AB_11150592), 1 μg/ml anti-hCD28 (Thermo Fisher Scientific ...