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Citations for BioLegend :
451 - 500 of 7268 citations for Estradiol Serum ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Conditioned medium was collected and IL-1β levels were assessed with ELISA MAXTM Deluxe Set Mouse IL-1β (Biolegend, 432604) following manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... and GM-CSF (purified anti-mouse GM-CSF; biotin anti-mouse GM-CSF) ELISA antibodies and standards were purchased separately from Biolegend.
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bioRxiv - Immunology 2023Quote: ... Tgf-β concentration was measured using ELISA (Total Tgf-β1 Legend MAX and Free Active Tgf-β1 Legend MAX, Biolegend). The limit of detection was 8 pg/mL for Tgf-β1 ...
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bioRxiv - Microbiology 2024Quote: ... TNF-α production was quantified in the supernatant of RAW 264.7 cells using a sandwich ELISA Max Deluxe Set (Biolegend) according to the manufacturers protocol.
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bioRxiv - Immunology 2022Quote: ... Cells were plated in a 24-well plate at a concentration of 500,000 cells/mL on either plate-bound α-CD3ε (2 μg/mL; 145-2C11; BioLegend) and soluble α-CD28 (1 μg/mL ...
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bioRxiv - Immunology 2024Quote: ... Mice CD4+ T cells (4x105/well in 96-well plate) were activated with plate-coated αCD3 (5 µg/ml, Biolegend) and αCD28 (10 µg/ml ...
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bioRxiv - Immunology 2019Quote: PBMCs were seeded in a 24 well plate at 2 × 106 cells/well in RPMI-1640 with anti-CD107a antibody (Biolegend, 1:200). Then ...
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bioRxiv - Bioengineering 2023Quote: ... T-cells that have been incubating with conditioned media were carefully transferred to the CD3-coated plates and further stimulated with 1 µg/mL anti-CD28 (BioLegend, clone: CD28.2) for another 48 hours ...
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bioRxiv - Microbiology 2023Quote: ... the plates were incubated with TMB substrate (BioLegend) for 15 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... they were stained with Zombie Violet™ Fixable Viability Kit (1:1000, BioLegend) for 30 minutes on ice during secondary antibody staining ...
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bioRxiv - Developmental Biology 2022Quote: ... were incubated with Zombie Aqua™ Fixable Viability Kit (Biolegend, #423101, 1:1000) for 15 min at room temperature ...
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PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiationbioRxiv - Developmental Biology 2023Quote: ... PE/Cy7-conjugated anti-mouse CD117 (c-Kit) antibody (1:200, Biolegend, 105814) and FITC-conjugated anti-mouse CD9 antibody (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... To exclude dead cells Zombie Aqua Fixable Viability Kit (#423102, 1:100, BioLegend) was added ...
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bioRxiv - Immunology 2022Quote: The concentration of IFN-γ was determined in the supernatant of 6×106 cells from each donor (6 healthy donors) using the Human IFN-γ ELISA deluxe set (BioLegend) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... TNFα and IL-23 expression in the culture medium was examined using an ELISA (Invitrogen for TNFα Biolegend for IL-23).
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bioRxiv - Cancer Biology 2023Quote: ... and IL-2 were investigated with an enzyme-linked immunosorbent assay (ELISA MAXTM Deluxe Set, Biolegend®, San Diego, CA, USA) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... that were pulsed with epitope (10 ng/mL) was collected and IFN-γ production was measured with an enzyme-linked immunosorbent assay (ELISA, BioLegend) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... serum was subjected to the LEGENDplex Mouse Inflammation Panel assay (BioLegend). Technical duplicates were averaged and compared to a standard curve according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... NK cells from HD or MM patients were stimulated in 96 wells plates with plate bound anti-CD16 (clone 3G8 Biolegend, 10ug/ml) or with IL-12 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... or co-stimulated with plate-bound CD3 (Biolegend #300333) and soluble CD28 (Biolegend #302943 ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were removed from their plates with Accutase (BioLegend) and counted ...
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bioRxiv - Immunology 2021Quote: ... AMs were lifted from plates using Accutase™ (BioLegend) and seeded for experiments.
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bioRxiv - Cell Biology 2021Quote: ... Azide-Free (LEAF) plate-bound anti-mouse CD3 (Biolegend) was used at 2 μg/ml and soluble anti-mouse CD28 (Biolegend ...
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bioRxiv - Biochemistry 2022Quote: Flat-bottom 96 well microtitre plates (Nunc MaxiSorp, Biolegend) were coated overnight at 4°C with either r3CLpro-RBD chimer (2 μg/mL) ...
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bioRxiv - Biochemistry 2022Quote: Flat-bottom 96 well microtitre plates (Nunc MaxiSorp, Biolegend) were coated with r3CL-pro ...
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bioRxiv - Immunology 2022Quote: ... Plates were again washed and TMB substrate solution (BioLegend) was added for 15 minutes at room temperature prior to addition of stop solution (2M hydrochloric acid) ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed and developed using TMB substrate (BioLegend). Reaction was stopped using 50μL of H2SO4 ...
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bioRxiv - Immunology 2022Quote: ... plate-bound antibodies (Abs) anti-NK1.1 (clone PK136; Biolegend), anti-NKG2D (clone A10 ...
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bioRxiv - Immunology 2023Quote: ... Plates were developed with 50 μl TMB (421101, BioLegend) and stopped using 50 μl 1.5 N H2SO4 ...
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bioRxiv - Immunology 2023Quote: ... stimulated with plate-bound anti-CD3 (Biolegend, 1μg/ml) and anti-CD28 (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM EDTA) and stained with Zombie Aqua Fixable Viability Kit (BioLegend, 1:200). Living ...
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bioRxiv - Immunology 2021Quote: ... fixed for 1 h at 25 °C using the True Nuclear fixation kit (BioLegend), washed twice with True Nuclear Permeabilization/Wash buffer ...
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bioRxiv - Immunology 2022Quote: ... fixed for 1 h at 25°C using the True Nuclear fixation kit (BioLegend), washed twice with True Nuclear Permeabilization/Wash buffer ...
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bioRxiv - Microbiology 2023Quote: ... Cytokine levels were determined using the human inflammation panel 1 LEGENDplex™ kit (Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... resuspended in 1 ml of PBS containing 1 µl live/dead stain (Zombie UV Fixable Viability kit (Biolegend, cat. no. 423107)) and incubated on ice for 30 min ...
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bioRxiv - Immunology 2022Quote: Supernatants were assessed for cytokine production using the LEGENDplex™ Human Inflammation Panel 1 (13-plex) in a V-bottom plate per the manufacturer’s instructions (BioLegend, San Diego, CA, USA). Flow cytometry data were acquired on a BD Fortessa analyzer (BD Biosciences ...
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bioRxiv - Biophysics 2023Quote: ... were isolated from buffy coats by density gradient centrifugation (Lymphoprep, StemCell ref: 07851) and activated on plates coated with anti-CD3 (1 mg/ml, Biolegend, ref: 317347, clone: OKT3) and anti-CD28 (1 mg/ml ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants thereof were removed and analyzed for IL-2 production as a correlate for T-cell activation using an ELISA MAX mouse IL-2 set (BioLegend, SanDiego, USA).
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bioRxiv - Immunology 2020Quote: ... The cytokine production was measured using the ELISA Deluxe Set Human IL-8/CCL20/IL-1β (Biolegend, San Diego, California, United States), following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2021Quote: Enzyme-linked immunosorbent assays were completed using and according to the instructions found with the ELISA MAX™ Deluxe Set Human IL-2 (BioLegend #431804).
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bioRxiv - Microbiology 2022Quote: Corning ELISA plate wells were coated with 100µL of 100 μg/mL FCoV2 whole virus antigen or 100 μg/mL SCoV2 RBD or FCoV RBD antigen in sodium bicarbonate ELISA coating buffer at pH 9.5 (BioLegend, San Diego, CA) and incubated overnight at RT ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected 3 days after coculture initiation and used to quantify T cell activation levels with ELISA (BioLegend, Cat no. 430801) following the manufacturer’s protocols.
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bioRxiv - Immunology 2023Quote: ... Co-culture supernatant was collected following completion of culture and analyzed for IL-2 using the ELISA MAX™ Deluxe Set for human IL-2 (Biolegend, 431804) at a dilution of 1:5.
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bioRxiv - Microbiology 2023Quote: ... PBMCs were then plated as described above and human IL-10 was measured in the cell supernatants by ELISA (Biolegend, Cat. # 430604).
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bioRxiv - Physiology 2019Quote: ... blocked with mouse serum and anti-murine CD16/32 (clone 2.4G2, Biolegend) and stained for cell surface markers (See Supplemental Table 1 for list of antibodies used) ...
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bioRxiv - Immunology 2019Quote: ... blocked with mouse serum and anti-murine CD16/32 (clone 2.4G2, Biolegend) and stained for cell surface markers (See Supplementary Table 2 for list of antibodies used) ...
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bioRxiv - Immunology 2023Quote: ... Serum cytokines were measured using the LEGENDplex Mouse Inflammation Panel (740446, BioLegend) bead-based immunoassay ...
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bioRxiv - Neuroscience 2021Quote: ... assays were performed using the in situ red starter kit according to the manufacture’s protocol with JADE1 antisera (1:20) and 0N tau antisera (1:500; BioLegend, San Diego, CA). Two control assays were also performed ...
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bioRxiv - Immunology 2021Quote: ... cells were restimulated with cognate antigens for three days and then supernatants were collected for IL-17A and IFNγ detection with ELISA (Biolegend, San Diego, CA). For determination of IL-17A+ and IFNγ+ CD45.1+ OT-II CD4+ T cell percentages ...
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bioRxiv - Immunology 2021Quote: Dead cells were stained using either Zombie UV™ Fixable Viability Kit (Biolegend, 1:1000), Fixable Viability Dye eFluor™ 506 (eBioscience ...