Labshake search
Citations for BioLegend :
601 - 650 of 2015 citations for 4 Chloro 2 fluoro 3' 4 methylpiperazinomethyl benzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: ... Primary antibodies were incubated overnight at 4°C in a humidified chamber: mouse anti-acetylated tubulin (1:400, BioLegend, 802001) and rabbit anti-GFP (1:500 ...
-
bioRxiv - Neuroscience 2024Quote: ... centrifuged at 1500 rpm for 5 min at 4°C and resuspended in 500µl Cell Staining buffer (BioLegend, Cat#: 420201) containing 1:4000 dilution of SYTOX Blue (ThermoFisher ...
-
bioRxiv - Synthetic Biology 2024Quote: ... high-binding 96-well plates were coated with 20 ng/well of RBD protein overnight at 4°C in coating buffer (421701, BioLegend), and the plates were blocked with 1% BSA in PBST for 1 h at 37°C ...
-
bioRxiv - Immunology 2024Quote: ... cells were centrifuged at 400 g for 5 minutes at 4°C and resuspended in cell staining buffer (CSB; Biolegend). The number of cells in the suspension was established using the CountessTM II cell counter (Invitrogen).
-
bioRxiv - Genomics 2024Quote: ... Primary antibody staining was carried out in the dark at 4 C for 30min with the following antibody dilutions in FACS buffer: 1:200 ICAM-1 (353102, Biolegend), 1:500 VCAM-1 (14-1069-82 ...
-
bioRxiv - Cancer Biology 2024Quote: ... ∼1×10.6 cells were stained with antibody cocktail at 4 °C for 20 min: Pacific Blue™ (PB) anti-mouse CD45.2 (Biolegend), APC anti-mouse B220 (Biolegend) ...
-
bioRxiv - Immunology 2024Quote: ... The cells were then stained for 30 mins at 4°C in the dark with anti-human antibodies specific for anti-CD14 [APC/Cyanine-7] (301819, BioLegend), anti-CD16 [Fluorescein isothiocyanate (FITC)] (360715 ...
-
bioRxiv - Neuroscience 2020Quote: ... Permeabilization was followed with blocking step by incubating the samples for 2 hr in PBS containing 3% normal goat serum (NGS, 927503, BioLegend) and 3% BSA and then stained overnight at 4°C with the primary monoclonal antibody (mouse anti-radixin ...
-
bioRxiv - Immunology 2020Quote: Recipient mice were infused with 2-3 x 106 purified donor T cells that were labeled with 5 μM CFSE (BioLegend) as previously described63 ...
-
bioRxiv - Immunology 2020Quote: ... Cocktail 2 was used to identify DE cells and contained 3 μl of anti-human CD5-APCeF780 antibody (clone L17F12, Biolegend), 2 μl of anti-human CD19-PECy7 antibody (clone HIB19 ...
-
bioRxiv - Cancer Biology 2022Quote: ... protein expression (ADT) and hashtag (HTO) libraries were generated using Chromium Single Cell 3’ version 2 reagents (10x genomics and Biolegend) per manufacturers’ protocols ...
-
bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were cultured alone or co-cultured with either of the B-cell populations at a ratio of 3:1 for 60 h at 37°C and 5% CO2 on anti-CD3 (2 μg/mL) (ULTRA-LEAF Biolegend) coated flat bottomed 384 CellCarrier Ultra microplates (PerkinElmer ...
-
bioRxiv - Immunology 2022Quote: ... resuspended in 50 μl FACS-buffer containing the respective fluorochrome-coupled Abs (anti-TACI/CD267 PE, clone eBio8F10-3, eBioscience, anti CD138 PECy7, clone 281-2, Biolegend, anti CD19 BV510 ...
-
bioRxiv - Immunology 2023Quote: ... Panels 2 and 3 incorporated anti-Digoxygenin (RD System, #611621), anti-FITC (Standard BioTools, #3174006B) and anti-Biotin (Biolegend, #409002) metal-tagged antibodies to detect the RNAscope staining output.
-
bioRxiv - Evolutionary Biology 2022Quote: ... Cells were centrifuged at 500× g at 4 °C and resuspended in 1/3 strength artificial seawater containing 2 μg/mL calcein AM (Enzo) and 100 nM sytox blue (BioLegend) to monitor viability ...
-
bioRxiv - Immunology 2024Quote: ... and APC anti-ligand (B7-H6: R&D, clone 875001; CD48: Biolegend, clone; BJ40; Nectin-2: Biolegend, clone TX31; LFA-3: Biolegend, cloneTS2/9 ...
-
bioRxiv - Cell Biology 2020Quote: ... Slides were incubated with the following primary antibodies diluted in blocking buffer overnight at 4°C – chicken anti-keratin 14 (1:800, Biolegend 906001), rabbit anti-SCD1 (1:500 ...
-
bioRxiv - Neuroscience 2021Quote: ... and then incubated with the following primary antibodies overnight at 4 : mouse anti-C3 (1:200 dilution; Biolegend cat. no. 846302) + rabbit anti-GFAP (1:500 dilutio L gilent Dako cat ...
-
bioRxiv - Immunology 2021Quote: Mouse B cells were bulk sorted from mixed chimeras into Eppendorf tubes containing cold sterile PBS 10% FCS and incubated for 20 min at 4°C with TotalSeq™ DNA-barcoded anti-mouse ‘Hashing’ antibodies (BioLegend) at a 1/100 final dilution ...
-
bioRxiv - Biochemistry 2020Quote: ... cells were incubated for 30 minutes at 4°C in PBS with a fluorochrome-conjugated antibody (mouse monoclonal APC-conjugated anti-CD69 (BioLegend 10909); mouse monoclonal PE-conjugated anti-CD25 (Pharmingen 30795X ...
-
bioRxiv - Bioengineering 2020Quote: ... anti-Pax7 (mouse monoclonal, DHSB, diluted 1:4) and anti-Neurofilament H phospho (rabbit polyclonal, BioLegend # PRB-573C, diluted 1:400). As secondary antibodies ...
-
bioRxiv - Developmental Biology 2021Quote: ... Blocking of Fc receptors was performed by incubation at 4°C for 20 minutes with an unlabelled anti-CD16/32 (1 μg/million cells; BioLegend – 101320). The cells were then incubated for one hour at 4°C in the dark with a mix of rat anti-mouse CD41 (labelled with Phycoerythrin ...
-
bioRxiv - Pathology 2022Quote: ... Cells were washed (PBS; twice), centrifuged (300g; 5min.; 4°C) and stained (10min., RT) with Streptavidin (Alexa Fluor® 647-conjugated, Biolegend, #405237 ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... The culture supernatants of stimulated cells were collected for cytokine (i.e., IL-4 and IFN-γ) assays using ELISA kits (BioLegend, CA).
-
bioRxiv - Immunology 2022Quote: ... 5ug/mL) and anti-CD28 (clone 37.51; 2ug/mL) in the presence of IL-4 neutralizing antibody (11B11, BioLegend, 5ug/mL) for 18-20 hours before the addition of cytokines as follows ...
-
bioRxiv - Microbiology 2020Quote: ... 5 × 105 cells/mL of CD14+ enriched cells were stimulated using recombinant human (rh) GM-CSF and rhIL-4 (BioLegend, USA) at a concentration of each 500 IU/mL in RPMI1640 media supplemented with 10% FCS at 37°C in 5% CO2 ...
-
bioRxiv - Neuroscience 2022Quote: ... The homogenates were first blocked in PBS with 10% rat serum with gentle rotation in a cold room and then stained for 1h protected from light at 4°C with CD206-FITC (cat no. 141704, Biolegend®) + MHCII-PE (cat no ...
-
bioRxiv - Immunology 2022Quote: ... prM-DENV2-treated PBMCs were treated (1h, 37 °C) in the presence or absence of anti-TNF-α antibody (4 µg/mL, BioLegend). The TNF-α depleted supernatants were used to stimulate HUVEC for 6 hours and the expression of activation markers were analyzed by flow cytometry ...
-
bioRxiv - Genomics 2022Quote: ... we stained cells with CITE-seq and hashing antibodies as previously described 22 (Supplementary Table 4) along with a fluorescent HLA antibody (BioLegend 311415). The 5% lowest expressing HLA cells were selected via FACS and 40,000 cells were loaded onto one 10x 3’ V3 Chromium channel ...
-
bioRxiv - Immunology 2020Quote: ... for 10 minutes at 4°C and then incubated for 30 min at room temperature with primary antibodies including CD45-BV785 (BioLegend, 103149) CD11b-PE (BioLegend ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were stained with the following primary mouse antibodies (1:100 dilution) for 30 minutes at 4°C: CD45-BV750 (clone 30-F11, Biolegend, 103157), CD31-PE-Cy5.5 (clone MEC 13.3 ...
-
bioRxiv - Microbiology 2020Quote: Splenocytes from intramuscularly vaccinated mice were incubated in culture with a pool of 253 overlapping 15-mer SARS-CoV-2 S peptides (Grifoni et al., 2020) for 12 h at 37°C before a 4 h treatment with brefeldin A (BioLegend, 420601). Following blocking with FcγR antibody (BioLegend ...
-
bioRxiv - Immunology 2020Quote: ... was incubated with 1-2 spike+ cells for 1 hour at 4°C before adding of 0.02 ug/ml ACE2-mFc followed by FITC conjugated anti-mouse IgG (Biolegend, cat#405305) and the MFI of FITC and positive percentage were collected.
-
bioRxiv - Immunology 2020Quote: ... cells were washed with PBS twice before staining with the following antibodies or reagents for another 30 min at 4°C in the dark: Streptavidin-BV650 (Biolegend, UK), IgG (H+L ...
-
bioRxiv - Immunology 2021Quote: Leukocytes from whole blood and ETA samples were incubated with oligo-conjugated Ig-A/D/G/M for 10 min at 4°C followed by the addition of Human TruStain FcX™ (BioLegend) and 10 min incubation at RT ...
-
bioRxiv - Immunology 2022Quote: ... IL-2 and IL-7 were removed from ILC2 culture and additionally cultured for 4 hr before restimulation with IL-33 (Biolegend, #580508) plus NMU (R&D systems ...
-
bioRxiv - Immunology 2022Quote: ... FcBlock was added for 10 min prior to fluorescent antibody staining for surface proteins for 30 min at 4°C using the following antibodies: BV510 anti-mouse CD45 (Biolegend 103137), FITC anti-mouse CD11b (eBioscience 11-0112-86) ...
-
bioRxiv - Neuroscience 2022Quote: ... Slices were incubated overnight at 4 °C with primary antibody (Biotin anti-β-Amyloid, 1-16 Antibody, clone 6E10 from BioLegend) dissolved in a carrier solution made with 1% HS ...
-
bioRxiv - Immunology 2022Quote: ... Cells were fixed with 4% paraformaldehyde (PFA) and stained intracellularly with IFN-γ-PE-Cy7 and Granzyme B-APC diluted in Perm-Wash buffer (Biolegend). Sample acquisition was performed on a Cytoflex-S (Beckman Coulter ...
-
bioRxiv - Immunology 2022Quote: ... Cells were fixed with 4% PFA and stained intracellularly with IFN-γ-PE-Cy7 and Granzyme B-APC diluted in Perm-Wash buffer (Biolegend). Sample acquisition was performed on a Cytoflex-S (Beckman Coulter ...
-
bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used in this study: Alexa Fluor 488 anti-human SSEA-4 (#330412, clone MC-813-70, Mouse IgG3 κ, dilution 1:100, Biolegend); Alexa Fluor 647 anti-human SSEA-4 Antibody (#330408 ...
-
bioRxiv - Immunology 2022Quote: ... Immunostaining was performed by incubating the sections overnight at 4°C with the SMI-32 primary antibody (Biolegend: Suppl. Table 1) in incubation buffer ...
-
bioRxiv - Immunology 2022Quote: ... Cells were then stained with fluorescent antibodies for intracellular proteins for 30 min at 4°C using the following antibodies: PE anti-mouse TNF (Biolegend 506306) and APC anti-mouse IL-6 (Biolegend 503810) ...
-
bioRxiv - Immunology 2022Quote: ... samples were pre-incubated for 5 minutes at 4°C with TruStain FcX™ (anti-mouse CD16/32) Fc block (clone 93; BioLegend). Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies ...
-
bioRxiv - Immunology 2022Quote: ... Cells were fixed with 4% PFA and stained intracellularly with IFN-γ-BV421 and TNFα-PE diluted in Perm-Wash buffer (Biolegend).
-
bioRxiv - Cancer Biology 2022Quote: ... for 10 min at 4°C followed by an incubation with CD45-BV421 (Biolegend 103133, clone 30-F11, 1:200 dilution) and Ter119-BV421 (Biolegend 116233 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Tissue sections were stained with monoclonal mouse anti-βIII-tubulin primary antibody (Biolegend, Cat. 801202; 1:100 overnight at 4 °C) and goat anti-mouse AF647 (abcam ...
-
bioRxiv - Cancer Biology 2022Quote: ... then incubated with their respective primary antibodies diluted in 5% skim milk in PBST at 4 °C overnight: purified mouse anti-βIII-tubulin (1:1,000) (BioLegend, cat. 801202), monoclonal mouse anti-GAPDH (1:50,000 ...
-
bioRxiv - Immunology 2024Quote: ... for 4 h and stained for intracellular expression of either IL-4 or FOXP3 after fixation and permeabilization using the Fixation Buffer (BioLegend, # 420801), the Intracellular Staining Perm Wash Buffer (BioLegend ...
-
bioRxiv - Developmental Biology 2024Quote: ... then incubated for 1 hr at 4°C with the following antibodies: CD26 (DPP4)-fluorescein isothiocyanate (FITC) (Biolegend, 137806; 1:200), anti-mouse ICAM1-phycoerythrin (PE)/Cy7 (Biolegend ...