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Citations for BioLegend :
6101 - 6150 of 6202 citations for NADH Cytochrome B5 Reductase 2 CYB5R2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Cell pellets were resuspended in 100 µL of Fc-blocking medium (5 µg/ml Purified anti-mouse CD16/32 Antibody (Biolegend, San Diego, CA) in FACS buffer) ...
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bioRxiv - Cell Biology 2024Quote: ... non-specific Fc receptor binding was blocked using 1:100 dilution of TruStain FcX anti-mouse CD16/32 antibody (BioLegend, RRID:AB_1574975, Cat#101320). 5 min after Fc blocker treatment ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the NK-92 staining panel: anti-CD107a (LAMP-1) FITC (BioLegend catalog #328606, clone H4A3), anti-CD56 (NCAM ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the PBMC staining panel for T cells: anti-CD4 FITC (BioLegend catalog #317408, clone OKT4), anti-CD26 PerCP/Cy5.5 (BioLegend catalog #356112 ...
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bioRxiv - Immunology 2024Quote: ... Cells were resuspended by mild vortexing and 50 ul of 2.5 µg/ml final (meaning 1/200 dilution of the stock) Fc-block (anti-mouse anti-CD16/32 antibodies, TruStain FcX from Biolegend, San Diego, CA) were added with the following addition of 1/200 dilution of antibodies for surface marker staining and Aqua Live/Dead dye (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... and activated for 72h with 10 µg/mL of plate-bound Ultra-LEAF™ purified anti-human CD3 monoclonal antibody (clone OKT3, BioLegend, cat. 317325) and 5 µg/mL of soluble Ultra-LEAF™ purified anti-human CD28 monoclonal antibody (clone 28.2 ...
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bioRxiv - Physiology 2024Quote: ... collected into FACS tubes (Falcon, cat no. 352052) and incubated with the following antibodies: AF700-labeled CD54 (1:100, BioLegend, cat no. 353126) and PE/Cy7-labeled CD62E (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated with primary antibody that recognizes an epitope within the humanized Aβ sequence using Purified Anti-B-Amyloid (BioLegend, Cat#803012, Lot#B251533) directly conjugated with horseradish peroxide (HRP ...
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bioRxiv - Immunology 2020Quote: ... ASC was stained using a primary antibody anti-ASC (polyclonal anti-PYD ASC, Santa Cruz or monoclonal anti-CARD ASC, Biolegend; 1:1000 dilution) or anti-Luciferase (Abcam ...
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bioRxiv - Microbiology 2019Quote: ... Cells were permeabilized with ice-cold methanol at −20°C for at least 20 min and incubated with anti-Pol II antibody (Biolegend, 8WG16 at 1:200), anti-PABPC antibody (Abcam ab21060 at 1:200 for mouse cells and Santa Cruz sc32318 at 1:25 for human cells ...
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bioRxiv - Cancer Biology 2019Quote: ... Sigma)for one hour at room temperature and incubated overnight at 4 °C with the following primary antibodies: anti-CD63 (BioLegend, San Diego, CA, USA), anti-Cytochrome c ...
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bioRxiv - Cell Biology 2020Quote: ... for 10 minutes at 4°C followed by staining with antibody cocktail containing Pacific-Blue-conjugated anti-mouse CD45 (clone 30-F11; Biolegend, San Diego, CA, USA), PE-conjugated anti-mouse Ly-6G (Gr-1 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated for 1 hour at 37 °C with different primary antibodies: anti-β-III-tubulin (1:4000, 801202, Biolegend, San Diego, CA), anti-neurofilament SMI312 (1:1000 ...
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bioRxiv - Bioengineering 2021Quote: The Zombie Aqua™ Fixable Viability dye and all primary antibodies used for flow cytometry analysis were obtained from BioLegend (San Diego, CA, USA) (Table 1).
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bioRxiv - Cell Biology 2021Quote: ... followed by the addition of 1:20 (1:40 final) primary antibodies against LAMP1 (AF488- or BV605-conjugated; BioLegend cat. no. 328610 and 328634), and/or TFRC (FITC-conjugated ...
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bioRxiv - Bioengineering 2022Quote: ... differentiated cells were again lifted off their culture surfaces using TrypLE Select and subjected to fluorescence activated cell sorting (FACS) after being stained with an erbB3 antibody (Biolegend, San Diego, CA, USA) conjugated to phycoerythrin ...
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bioRxiv - Cancer Biology 2022Quote: ... was analyzed and monitored longitudinally by weekly bleeding to quantify hCD45+ cells in the peripheral blood by flow cytometry with human CD45-PE and anti-mouse CD45-APC-Cy7 antibodies (Biolegend, San Diego, CA, USA). Mice were monitored closely to detect disease onset and treatment started when hCD45+ cells were detectable in the peripheral blood ...
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bioRxiv - Cancer Biology 2022Quote: ... Human tissues were incubated for 25 min at 4 °C with the following primary antibodies: CD24-PE (BioLegend, clone ML5, Cat, #: 311105, 1:100), CD44-FITC (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... Staining for human immunoglobulins was performed in 100 μL with 1:50 (v/v) of the detection antibodies: anti-human IgM Brilliant Violet 650 (clone: MHM-88, Biolegend® Cat. No. 314526), anti-human IgG PE/ Dazzle™ 594(clone ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in FACS buffer and stained on ice for 30 minutes with an Alexa647-labelled anti-human IgG-Fc antibody (Biolegend, San Diego, USA, #409320). Alexa647 mean fluorescence intensities were determined for GFP-positive HEK-293T cells with a FACS Attune Nxt and analyzed with the software Flow Logic™ (IInivai Technologies Mentone ...
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bioRxiv - Immunology 2020Quote: ... IgM (PE), SIRPα (APC or PerCP 710) (all antibodies purchased from eBioscience, San Diego, CA, USA) and CD43 (APC Cy7, BioLegend, San Diego, CA, USA). Expression of proteins was measured using FACS Canto II HTS (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Staining was then performed at 4°C for 30 minutes with a surface stain cocktail containing the following antibodies: CD4 APC (BioLegend, cat. 100412, clone GK1.5), CD8 Alexa Fluor 700 (BioLegend ...
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bioRxiv - Cancer Biology 2019Quote: ... blocked with 10% BSA in PBS and incubated with 1μg of an Alexa-Fluor® 488-conjugated anti-phospho-histone H3 (Ser10) antibody (Cat# 650804, BioLegend, San Diego, CA, USA). After washing ...
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bioRxiv - Genomics 2019Quote: ... The following antibodies were used for these studies: anti-human CD47-APC (1µl/106 cells in 100µl; clone CC2C6, BioLegend, Cat. #323123/4, RRID: AB_2716202/3), anti-human CD47-FITC (5µl/106 cells in 100µl ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained in the dark on ice for 30 min with 0.5 μl anti-CD62L and anti-CD69 antibodies conjugated to PE and APC respectively (BioLegend, clones DREG-56 and FN50) in 100 μl FACS buffer per well ...
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bioRxiv - Cell Biology 2020Quote: ... Surface marker staining was performed for 30 min on ice with the following antibodies: CD34-eFluor 660 (eBioscience, clone RAM34, 1:100) and ITGA6-PE/Cy7 (Biolegend, clone GoH3, 1:1000) for sorting or CD34-eFluor 660 (eBioscience ...
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bioRxiv - Immunology 2020Quote: ... and incubated with FC block and subsequently stained with a combination of the following anti-mouse antibodies (all from BioLegend, eBioscience, or BD Biosciences): anti-CD8α (53-6.7) ...
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bioRxiv - Immunology 2021Quote: ... Cells were suspended in FACS buffer and stained for 30 min at 4 °C with the desired antibody cocktails (BioLegend, San Diego, CA, US) of APC-Fire 750-anti CD11b (Cat ...
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bioRxiv - Genomics 2021Quote: ... 5% normal goat serum in PBS for 1 hour and incubated at 4°C overnight with primary antibody (Biolegend, cat. 613402, dilution 1:500), then at room temperature for 3 hours with secondary antibody (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2021Quote: ... CD8+ T cells isolated at each time point of interest were directly stained in round-bottom 96-well plates using the following surface-marker antibodies (either from eBioscience, BioLegend, or produced in house): anti-mouse CD8a FITC (clone 53.6.7 ...
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bioRxiv - Immunology 2021Quote: ... The resulting whole-splenocyte single-cell suspensions were directly stained in round-bottom 96-well plates using the following surface-marker antibodies (either from eBioscience, BioLegend, or produced in house): anti-mouse CD8a FITC (clone 53.6.7 ...
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bioRxiv - Cancer Biology 2022Quote: ... the antibody or IgG1 excess was rinsed and pellets were resuspended in APC-conjugated Annexin V at 1:20 in Annexin V buffer solution plus Zombie Violet dye at 1:400 (all antibodies and probes are from Biolegend, San Diego, CA, USA). Samples were transferred into FACS tubes and incubated for 20 minutes at room temperature protected from light prior to their analysis by FACS Canto II (BD ...
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bioRxiv - Immunology 2022Quote: Antibody staining was done in the dark for 30 minutes at 4°C in 30 μL of FACS buffer with antibodies and anti-CD16/32 (TruStain FcX™ PLUS; BioLegend, #156604; 1:100). Acquisition was done on a BD FACSCanto™ II or BD LSRFortessa (both BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... Staining was then performed at 4°C for 30 minutes with CR3022 or 4A8 and a surface stain cocktail containing the following antibodies: CD169 PerCP/Cy5.5 (BioLegend (San Diego, CA], cat. 142410), SIGN-R1 PE (R&D Systems [Minneapolis ...
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bioRxiv - Molecular Biology 2023Quote: ... Murine anti-V5-tag (clone 7/4, #680602) and rabbit anti-V5-tag (Polyclonal, #903801) antibodies were purchased from BioLegend (San Diego, CA, USA). Anti-V5-tag mAb-magnetic beads (#M167-11 ...
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bioRxiv - Immunology 2023Quote: ... Sections were then stained with primary antibody diluted in 1% BSA in PBS for 1 hour with rat anti CD68 (BioLegend 137002 1:400 dilution), rabbit anti Lyve1 (abcam ab14917 1:200 dilution) ...
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bioRxiv - Bioengineering 2024Quote: ... The sections were subsequently stained with a rat anti-human YAP antibody conjugated with PE (dilution: 1:500; clone W19260A; Biolegend, San Diego, CA, USA) for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then stained for 30 minutes at room temperature with a panel of 138 Total-Seq-C antibodies (Biolegend, Stoeckius et al 2017) and washed 3x using the HT1000 laminar wash system (Curiox) ...
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bioRxiv - Immunology 2024Quote: ... Isolated CD4+ T-cells were either maintained in a naïve state by seeding in complete growth medium alone or underwent TH1-activation in tissue culture flasks pre-coated with anti-human CD3 antibody (10 µg/mL) (BioLegend, San Diego, CA, US) in complete medium supplemented with anti-human CD28 antibody (5 µg/mL ...
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bioRxiv - Immunology 2024Quote: ... Single cell suspensions were labelled with antibodies against cell surface antigens as described above and then prepared using a Cyto-Fast Fix/Perm kit (BioLegend, San Diego, CA, US), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... peripheral blood and spleens were harvested from each mouse and the infiltrated primary cells were identified using flow cytometry by staining for human CD45 antibody (BioLegend; BLG-368522; 1:200).
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bioRxiv - Immunology 2023Quote: ... and then stained for 30 minutes at 4 °C using the following antibodies: Brilliant Violet 711™ anti-human CD4 (344648, BioLegend; 1 μL/well), APC anti-human CD3 (300412 ...
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bioRxiv - Pathology 2023Quote: ... Thereafter the sections were incubated overnight at 4°C with primary antibody (the primary antibodies for C3 and CFH were monoclonal from Santa Cruz raised in mouse and for NF-KB the monoclonal primary antibody was from Biolegend raised in mouse) diluted in 1X PBS followed by washing with 1X PBS and incubation for 60 minutes with biotinylated anti-mouse immunoglobulin ...
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bioRxiv - Immunology 2022Quote: ... Transfectants were stained for viability using a Live dead blue dye followed by anti-HLA DR DP DQ antibodies (BioRad clone Bu26, Cat. #MCA2497F; Beckman clone I3, Cat. #CO6604366; BioLegend, clone Tü39, Cat. #361704) or anti-HLA DP (Leinco ...
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bioRxiv - Immunology 2022Quote: ... 4 °C) cells were stained with the following conjugated antibodies for 20 min at RT in the dark: CD3-APC (Biolegend, clone REA613, 1:200), CD11b-APC-Cy7 (Biolegend ...
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bioRxiv - Neuroscience 2023Quote: ... were blocked with 5% BSA and 5% goat serum in PBST (PBS/0.25% Triton X-100) for 30 min and then incubated with monoclonal 6E10 antibody (BioLegend, cat# 803001, 1:1000 dilution) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.0 × 106 cells were incubated at room temperature for 20 min with 5 µL of APC-labeled anti-CD45 antibody (Clone: 2D1, BioLegend, San Diego, CA, USA). The cell lines were washed in PBS containing 2% FBS ...
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bioRxiv - Bioengineering 2023Quote: ... the slices were transferred into 30% sucrose in water for >24 hours and then sub-sectioned on a sliding microtome to 15-30 μm for immunostaining using the following antibodies: mouse anti-HA (Biolegend catalog #901513, 1:1000) and rabbit anti-NeuN (Millipore catalog #ABN78 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were stained for one hour using the following antibodies: Allophycocyanin (APC) anti-human CD274 (5 μL/test; BioLegend #329707; BioLegend, San Diego, CA, USA), APC anti-mouse CD274 (5 μL/test ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed 3 x with 1% BSA before incubating for 1 h in anti-human IgG secondary antibody labelled with either APC (BioLegend, #410712, dilution 1:100) or Phycoerythrin (Abcam ...