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Citations for BioLegend :
6001 - 6050 of 6202 citations for NADH Cytochrome B5 Reductase 2 CYB5R2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Sections were stained using a three-colour protocol with 1:100 of each of the following antibodies in IF blocking buffer at 4 °C O/N: Alexa Fluor 488® anti-mouse GL-7 (GL-7; Biolegend, #144612), PE anti-mouse CD3 (145-2C11 ...
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bioRxiv - Biophysics 2023Quote: ... 100 μL of PRP was incubated with 1 μg/mL AF647-conjugated CD42b rabbit anti-human antibody (BioLegend, San Diego, CA, USA) to label the platelets ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were incubated with antibody cocktails in blocking buffer overnight at 4°C in a moisture chamber: Amyloid-β (6E10) (1:100; Biolegend, 803001), anti-CHMP2B (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... were rehydrated in distilled H2O then stained with Nissl or immunohistochemical labelling was performed as above with anti-mouse Ly6G primary antibody (BioLegend, London, UK) and goat anti-rabbit secondary antibody.
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies were resuspended in 0.1% Tween-20 in PBS supplemented with 5% BSA at the following dilutions: TUJ1 (BioLegend, 801202, 1:400), SOX2 (Invitrogen ...
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bioRxiv - Immunology 2023Quote: Freshly sorted CD8+ T lymphocytes of four healthy volunteers were labelled with anti-human Hashtag antibodies TotalSeq-C (Biolegend, San Diego, CA) and were pooled together ...
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bioRxiv - Genetics 2023Quote: ... were isolated by 1X Red Blood Cell lysis Buffer (eBioscience) and stained with a combination of fluorochrome-conjugated antibodies: c-Kit (BD Biosciences, BioLegend clone 2B8), Sca-1 (BioLegend clone D7) ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated for the period of activation expansion with 20µg/ml of anti IL-10 antibody (JES5-2A5; Biolegend catalog No. 504908) or corresponding isotype control (Biolegend catalog No ...
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bioRxiv - Cancer Biology 2023Quote: ... Single-cell suspension from mouse tumors or spleens were stained with Alexa Fluor 700 anti-mouse CD45 Antibody (Biolegend, USA, Cat#147716), APC anti-mouse F4/80 Antibody (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... This additional step removes the likelihood of TCR α-chain and β-chain mispairing and allows us to evaluate TCR-transduction efficiency by staining with anti-murine TCR constant region antibody (Clone H57-597; BioLegend Cat. #109208). Flow sorting was performed to isolate highly-transduced CD8+ T-cells by selecting for cells stained strongly with anti-CD3 and anti-murine TCR constant region antibody ...
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bioRxiv - Cell Biology 2023Quote: ... The protein mixture was then added to 800-nm diameter carboxylated polystyrene beads that were coated with a rabbit polyclonal anti-GFP antibody (BioLegend MMS-118P), and incubated for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... and stained overnight with Alexa 488-conjugated Alexa Fluor® 488 anti-tubulin β3 and Alexa Fluor® 647 anti-mouse/human CD324 (E-cadherin) antibodies (#801203 and #147308, BioLegend). Each batch of anti-tubulin β3 antibody was titrated before use to minimize background staining ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 30 minutes at 4 °C using antigen probe (1.5 μL/well) and the following conjugated antibodies: APC anti-human CD3 (300412, BioLegend; 1 μL/well), PerCP/Cyanine 5.5 anti-human CD19 (302230 ...
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bioRxiv - Cancer Biology 2023Quote: ... the tumor organoids were treated with the immune checkpoint inhibitor Nivolumab (Selleckchem, Cat#A2002) and the Ultra-LEAF Purified Human IgG4 Isotype Control Recombinant Antibody (Biolegend, Cat#403701) at a concentration of 10 μg/mL ...
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bioRxiv - Microbiology 2024Quote: Suspensions of ∼106 HIV-infected TMCs in PBS with 1% FBS were incubated with BV711 anti-human CD3 antibody (Biolegend, catalog # 300464) at 4°C for 30 min ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the PBMC staining panel for purity: anti-CD4 FITC (BioLegend catalog #317408, clone OKT4), anti-CD20 PerCP/Cy5.5 (BioLegend catalog #375510 ...
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bioRxiv - Immunology 2024Quote: ... and PBS-57/CD1d-tetramers (NIH Tetramer Facility) for 20 mins and subsequently stained with the surface antibodies against CD3 (Biolegend cat #: 300324), CD4 (BD cat #:611887) ...
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bioRxiv - Molecular Biology 2024Quote: ... the antibody (CD8 clone RPA-T8; Biolegend Cat: 301002; CD4 clone RPA-T4; Biolegend Cat: 300502; CD3 clone UCHT1; Biolegend Cat: 300402) is incubated with DBCO-Peg5-NHS Ester (linker ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with the following antibody cocktail diluted in flow cytometry staining buffer: mouse anti-human CD3 BV750 (BioLegend Cat 344846), mouse anti-human CD4 AF700 (BioLegend Cat 300526) ...
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bioRxiv - Immunology 2024Quote: ... mice were injected twice with 250 µg of either the isotype control IgG (Ultra-LEAFPurified Rat IgG1, λ Isotype Ctrl Antibody no. 401916, Biolegend, CA) or PC61 anti-CD25 monoclonal antibody (Ultra-LEAF Purified anti-mouse CD25 Antibody no ...
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bioRxiv - Neuroscience 2024Quote: ... cell preparation and cell surface marker staining was carried out as previously described.20 The following antibodies were used for staining: mouse anti-CD11b (Biolegend, Cat# 101257), mouse anti-CD19 (Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies and channels were used to differentiate leukocyte populations CD4 BV510 (Biolegend Cat. No 100553, Clone RM4-5 0.038ng/µl), CD45 APC (Biolegend Cat ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies were used for flow cytometry: Brilliant Violet 510-conjugated anti-CD19 (clone RA3-6B2, BioLegend, San Diego, CA, USA), phycoerythrin (PE)-Cy7-conjugated anti-CD3ε (clone 145-2C11 ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa Fluor® 700 anti-human CD41 antibody and Zombie NIR® fixable dye were obtained from BioLegend (San Diego, CA, USA). Anti-human CD62P conjugated PE antibody was obtained from Beckman Coulter (Brea ...
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bioRxiv - Neuroscience 2024Quote: ... Fixation and cryostat sectioning of 3D organoids was performed as previously described.47 Immunostaining was done using antibodies to PAX6 (BioLegend, 1:200), SOX2 (Santa Cruz ...
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bioRxiv - Immunology 2021Quote: ... cells were stained in PBS at 4°C for 30 minutes with the fixable viability dye eFluor 780 (1:1000, 65-0865-14 eBioscience) and a combination of the following antibodies (1:200, all from BioLegend unless stated otherwise): anti-mouse CD45 BV650 (30-F11 ...
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bioRxiv - Immunology 2021Quote: ... Nonspecific binding was blocked by incubation for 10 min at 4 °C with anti-CD16/CD32 antibody (Biolegend, clone 93, 5 ng/ μL) antibody and stained with the appropriate antibodies for 15 min at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... blotted membranes were blocked with 5% milk in TBST for 30 minutes and probed overnight (4°C) with primary antibody (anti-β-actin [Sigma-Aldrich, A5441], anti-HA [Biolegend, 901501]). After 3 washes with TBST ...
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bioRxiv - Immunology 2022Quote: ... followed by flow cytometric analysis of the uptake of fluorescent OVA and the presentation of the OVA257-264 (SIINFEKL) by mouse MHC-I (H2Kb) using a PE-conjugated anti-H2Kb-SIINFEKL antibody (Biolegend; clone 25-D1.16). To further confirm the role of S100A4 in promoting antigen cross-presentation ...
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bioRxiv - Genomics 2020Quote: ... Cells were blocked with 1% BSA/PBS for 30 minutes and then incubated with antibodies for HA.11 (BioLegend MMS-101P, 1:2000) and BIP (Abcam ab21685 ...
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bioRxiv - Immunology 2019Quote: ... The stain solution contained a 1:200 dilution of an antibody against H-2Kb bound to SIINFEKL conjugated to PE (#141603, Biolegend, San Diego, CA) to detect SIINFEKL peptide being presented at the cell surface ...
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bioRxiv - Genetics 2019Quote: ... The cells were stained with the following antibody cocktail: anti-CD4-APC (30 µl/ml final volume, clone OKT4, Cat. no. 317416, BioLegend, San Diego, U.S.), anti-CD127-FITC and (30 µl/ml ...
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bioRxiv - Immunology 2019Quote: ... Platelet count and platelet activation were determined by flow cytometry (FACS Calibur, Becton Dickinson, Franklin Lakes, NJ) using hamster anti-CD61-APC monoclonal antibody (BioLegend, San Diego, CA) and anti-CD62p-FITC (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: The following antibodies were used for the analysis of human synovial cells with cell sorting: APC anti-CD3 (Clone OKT3, BioLegend Cat# 317317, RRID:AB_1937213), PE anti-CD45RO (Clone UCHL1 ...
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bioRxiv - Microbiology 2021Quote: ... 20 μl cells were removed for flow analysis and remaining cells were incubated for 30 min on ice with 1 μg each of TotalSeq™-A0441 anti-mouse CD71 Antibody (Biolegend, Cat# 113824) and TotalSeq™-A0073 anti-mouse/human CD44 Antibody (Biolegend ...
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bioRxiv - Bioengineering 2021Quote: ... Anti-FLAG epitope (DYKDDDDK, catalog# 637301) and horseradish peroxidase (HRP) Donkey anti-human IgG antibody (catalog #410902) were purchased from Biolegend (San Diego, CA). The secondary antibody anti-rat IgG HRP (catalog# 7077 ...
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bioRxiv - Bioengineering 2021Quote: ... 22 and 28 before differentiation of mast cells was assessed by flow cytometry with antibody markers for CD117 APC (2B8, Biolegend, San Diego, CA) and FcεRI PE (MAR-1 ...
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bioRxiv - Microbiology 2020Quote: ... After 48h of co-infection the cells were harvested and processed as above using an anti-MHC-II FITC-conjugated antibody (Biolegend San Diego, CA). Treatment with PMA (50 ng/ml ...
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bioRxiv - Neuroscience 2020Quote: ... 0.05% Sodium Azide in PBS) and incubate overnight at 4°C with the monoclonal anti-amyloid beta antibody 6E10 (Biolegend, 803001, lot. B228658) at a concentration of 3μg/ml (Antibody buffer ...
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bioRxiv - Microbiology 2021Quote: ... incubated 15 minutes on ice and then stained at RT in the dark for 20 minutes with the following mixture of fluorescent-labeled antibodies: CD3-APC (Biolegend, San Diego, CA), CD4-BV510 (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: ... Aβ peptides were extracted from 250 µL human plasma using immunoprecipitation with anti-β-Amyloid 17-24 (4G8) and anti-β-Amyloid 1-16 antibodies (6E10, both Biolegend®) coupled to Dynabeads™ M-280 Sheep Anti-Mouse IgG magnetic beads (Thermofisher ...
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bioRxiv - Cancer Biology 2020Quote: ... We split our single cell suspension in half and stained half of the uncultured HCI010 and HCI002 cells for FACS using fluorescent labeled antibodies for human specific CD298 (Biolegend, cat. no. 341704) and mouse-specific MHC-I (eBioscience ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were stained for 30 min at +4°C with APC or PE-conjugated anti-CD133 antibodies (diluted at 1/200 or 1/400 respectively; Biolegend, San Diego, USA) or corresponding control Immunoglobulin G1 (IgG1 ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were stained for 20 minutes on ice with 1µg of BioLegend TotalSeq-A anti-human hashtag oligo antibody (BioLegend TotalSeq-A0251 to A0258). Cells were washed 3 times then pooled at equal ratios for sequencing ...
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bioRxiv - Neuroscience 2022Quote: ... Splenocytes were then filtered through a 40μM filter and stained with an antibody cocktail [Pacific Blue anti-CD45.1 (Biolegend no. 110722; 1:100 dilution), APC anti-CD45.2 (Biolegend no ...
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bioRxiv - Genomics 2022Quote: ... CD45+ tumor-infiltrating leukocytes were stained with 2.5 μg/ml of Totalseq anti-mouse Hashtag-A antibodies (A0301-A0314, BioLegend, San Diego, CA, USA) at 4°C for 25 minutes and washed thrice with Cell Staining Buffer (BioLegend ...
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bioRxiv - Genomics 2022Quote: ... Bone marrow mononuclear cells were stained for 30 minutes on ice with the following antibodies: anti-CD19(APC/Cy7) (clone HIB19; Biolegend; Cat. num. 302217) in a 1:20 dilution ...
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bioRxiv - Immunology 2022Quote: ... CD11bhi, F4/80hi) were isolated by FACS using antibodies against CD11b (eBioscience, #11-0112, 1:1600) and F4/80 (Biolegend, #123114, 1:200), and total RNA was extracted using RNeasy Plus Mini Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed in PBS/1% BSA buffer and incubated for 30 minutes on ice with the following antibodies from Biolegend (San Diego, USA): FITC-anti-human CD38 (#303503) ...
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bioRxiv - Cell Biology 2022Quote: ... single cell suspensions of WT intestinal epithelium were stained with CytoID as detailed above and incubated with anti-mouse Hashtag antibodies (BioLegend Cat #155839, 155841,155843,155845). CytoID high and low cells were then sorted and processed separately for sequencing library generation ...