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Citations for BioLegend :
6001 - 6050 of 6161 citations for Ectonucleotide pyrophosphatase phosphodiesterase family member 7 ENPP7 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... cellular suspensions isolated from bone marrow were stained at 4°C in the dark in FACS buffer with lineage markers including phycoerythrin (PE) anti–mouse antibodies directed against directed against B220 (BioLegend, clone RA3–6B2), Cd11b (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... was used to detect T cells and the following anti-human antibodies were used to identify T cell subsets: anti-CD137-PeCY7 (BioLegend Cat# 309818, RRID:AB_2207741), anti-CD103-BV605 (BioLegend Cat# 350218 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated for 30 min at 4°C with pre-conjugated fluorescent labeled antibodies with the following combinations: CD45 (BioLegend, 368532 (PE/Cyanine7)) ...
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bioRxiv - Neuroscience 2020Quote: ... anti-neurofilament to a phosphorylated heavy chain neurofilament (NF-H; mouse anti-human monoclonal antibody SMI-34, dilution 1:100, epitope retrieval time 10 min, Biolegend 835503; Biolegend, San Diego, CA). Hemosiderin deposition was evaluated on a section with only counterstain ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated with Fc-blocking anti-CD16/32 anti-bodies followed by cell hashing antibody (1 μg/ml, BioLegend Cell Hashing A) (Stoeckius et al. ...
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bioRxiv - Immunology 2020Quote: Mice were bled from the submandibular vein and the erythrocytes in the blood were lysed with ACK lysis buffer and stained with the following antibodies: mouse-CD45-PECy7 (BioLegend; clone 30-F11), human-CD45-Pacific Orange (Thermo Fisher ...
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bioRxiv - Genomics 2021Quote: ... Sections were incubated with the following primary antibodies at the indicated dilution in blocking buffer overnight at 4°C: NeuN (BioLegend 1B7, cat #834502), p65 (BioLegend Poly6226 ...
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bioRxiv - Immunology 2021Quote: ... Cells were recovered via centrifugation and stained for flow cytometric analysis as described above using the following fluorochrome-conjugate monoclonal antibodies: TIM4 (BioLegend, clone: F31-5G3), CD45.1 (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... stained for 20 minutes on ice with the following antibodies at the following concentrations: anti-mouse CD49b 1:500 (#103511 Biolegend, San Diego, CA), anti-human CD49b 1:750 (#359310 Biolegend) ...
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bioRxiv - Cancer Biology 2022Quote: CAR-T cells were co-cultured with tumor at ratio of 2:1 for 6 hours at 37o C in CO2 incubator with CD107a antibody (Biolegend, 328620, clone-H4A3) and golgistop (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then resuspended in 50 µl of flow buffer (1x PBS, 2% FBS, 10mM EDTA, 25mM HEPES) containing anti-CD16/CD32 antibody for Fc receptor blockade (Biolegend, 101320, 1:200) and incubated on ice for 5 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... a sequential gating strategy was used to analyze cancer cells by staining with the following FACS antibodies (Figure S3F): CD45-Pacific Blue (BioLegend 103126, 1:100), CD31-Pacific Blue (BioLegend 102422 ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were incubated with Live/Dead fixable violet dead cell stain [Thermo Fisher Scientific (Waltham, MA)] for the live cell gating and with the following antibodies from Biolegend (San Diego, CA): CD16/32 ...
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bioRxiv - Immunology 2022Quote: Activated γδT cells were seeded in round-bottom plates with FITC conjugated anti-mouse CD107a (1D4B) antibody or anti-human CD107a (H4A3) (Biolegend, San Diego, CA). γδT cells were co-cultured with A20 or K562 at a ratio of 1:1 ...
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bioRxiv - Immunology 2022Quote: ... Circulating maternal neutrophils were depleted at days 5 (E4.5) and 8 (E7.5) of pregnancy using a monoclonal neutralizing antibody (Biolegend, clone 1A8; 50 μg i.v.) or isotype control (Biolegend clone RTK2758 ...
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bioRxiv - Microbiology 2022Quote: ... Positive clones were isolated by limiting dilution and integration of the U1 construct was confirmed with immunofluorescence microscopy and western blotting using an anti-HA antibody (Biolegend cat. no. 901501). Parasite lysate was not boiled prior to western blotting ...
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bioRxiv - Neuroscience 2023Quote: ... blood cells were lysed using VersaLyse buffer and blood and CSF cells were stained using the following anti-human antibodies (Biolegend; clone names indicated): CD3 (UCHT1) ...
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bioRxiv - Cancer Biology 2023Quote: ... and stained overnight at 4°C in blocking solution with the following primary antibodies and dilutions: anti-Nestin (1:150, Biolegend, #656802, clone 10C2), anti-EGFR (1:300 ...
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bioRxiv - Microbiology 2023Quote: ... A combination of selected antibodies (anti-mouse) was used depending on the purpose of each study: CD45 APC-Cy7 (Biolegend, clone 30-F11), CD8a FITC (Tonbo ebioscience ...
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bioRxiv - Molecular Biology 2023Quote: ... and spleen were counted on a Pentra ES60 Haematology Analyser (Horiba) and stained with anti-mouse antibodies against the following antigens as appropriate (all from BioLegend unless otherwise stated) in FACS buffer (PBS with 2% BSA and 0.025% sodium azide) ...
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bioRxiv - Molecular Biology 2024Quote: ... according to manufacturer’s recommendations and stained for 1 hour at 4°C with the following antibodies: NGFR (345110; 1:600, BioLegend, San Diego, CA, USA), α-tubulin (NB100-69AF405 ...
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bioRxiv - Cell Biology 2024Quote: ... utilizing two distinct antibody panels with or without PE-anti-cMPL (Miltenyi Biotech) or PE-anti-cKIT (BioLegend, San Diego, CA, USA, 105807). The human HSC panel included FITC-anti-CD34 (BD ...
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bioRxiv - Immunology 2024Quote: ... Cell samples were stained with the following antibodies and stains (in multiple sets to prevent fluorophore overlap): B220-FITC (BioLegend, clone RA3-6B2), CD11c-PacBlue (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... Aliquots were thawed and incubated with the panel of antibodies following the blockade of non-specific binding of the immunoglobulin to the Fc receptors by TruStain FcX (BioLegend, San Diego, CA). LIVE/DEAD Fixable Near-IR Dead Cell Stain dye (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The following monoclonal antibodies were used for flow cytometry analyses at a dilution of 1/700: anti-CD45 (BioLegend, clone 30-F11, 103147), anti-CD11b (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... in 6-well plates in a total volume of 3mL of medium and collected after exposure/drug treatment via trypsin and trypsin-neutralizer and according to manufacturer protocol using FITC anti-BrdU antibody (Biolegend, San Diego, CA). Flow cytometric measurements were performed on a FACS Fortessa (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... All cytokine antibodies were used at 1:100 dilution in 1x BD Perm/Wash buffer (anti-IFN-γ (APC/Cyanine7 or PE, Biolegend, #505849, #505808) and anti-TNF-ɑ (BV711 Biolegend ...
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bioRxiv - Molecular Biology 2023Quote: Populations of NDFs or HT1080 cells expanded after electroporation with approximately 50% of 53-positive and p53-negative cells were treated with 1 μM CBL0137 or 500 μg/ml bleomycin for 24 hr and then labeled with hashtag antibody oligonucleotide conjugate (HOT) from Biolegend (San Diego, CA) as follows ...
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bioRxiv - Immunology 2023Quote: Purified and biotinylated IL-6 (504502 and 504601, respectively) and IL-10 (505002 and 504906, respectively) antibodies were purchased from Biolegend (San Diego, CA). Purified antibodies were used to coat an ELISA plate ...
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bioRxiv - Neuroscience 2023Quote: ... Extracellular deposits of Aβ peptide were immunostained with the mouse anti-β-amyloid 17-24 antibody (1:5000, clone 4G8, BioLegend, Koblenz, Germany). Alpha-synuclein pathology was detected with the anti-syn-1 antibody (1:2000 ...
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bioRxiv - Immunology 2023Quote: ... prior to the end of the CD45- sort one vial of TotalSeq-C Human Universal Antibody Cocktail v1.0 (Biolegend, San Diego, CA, USA) was equilibrated to room temperature for 5min and then spun at 10,000g for 30 seconds ...
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bioRxiv - Immunology 2023Quote: ... peripheral blood or BM were incubated for 20 min at 4°C with the following anti-mouse conjugated antibodies: anti-CD45-PerCP/Cy5.5 (BioLegend, clone 30-F11, #103132), anti-CD45.1-PE/Cyanine7 (BD Biosciences ...
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bioRxiv - Developmental Biology 2023Quote: ... 10 mg of protein G agarose beads were crosslinked to 5 μL of mouse anti-HA antibody (BioLegend, 901501, 1:1000, RRID: AB_2565006) using an effective concentration of 6.5 mg mL-1 DMP ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were used to validate siRNA knock-down in fibroblasts: anti-α2-spectrin (mouse IgG2b clone D8B7, Biolegend #803201, 1:1000), anti-β2-spectrin (BD Biosciences #612563 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were suspended at 3×106 cell/ml and incubated for 30 min at 4C with 1:100 dilutions of the following antibodies: [APC dump channel: Ter-119 (Biolegend cat no. 116212), CD45 (Biolegend cat no ...
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bioRxiv - Immunology 2024Quote: ... Cells were resuspended by mild vortexing and 50 ul of 2.5 µg/ml final (meaning 1/200 dilution of the stock) Fc-block (anti-mouse anti-CD16/32 antibodies, TruStain FcX from Biolegend, San Diego, CA) were added with the following addition of 1/200 dilution of antibodies for surface marker staining and Aqua Live/Dead dye (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... and activated for 72h with 10 µg/mL of plate-bound Ultra-LEAF™ purified anti-human CD3 monoclonal antibody (clone OKT3, BioLegend, cat. 317325) and 5 µg/mL of soluble Ultra-LEAF™ purified anti-human CD28 monoclonal antibody (clone 28.2 ...
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bioRxiv - Cell Biology 2024Quote: ... non-specific Fc receptor binding was blocked using 1:100 dilution of TruStain FcX anti-mouse CD16/32 antibody (BioLegend, RRID:AB_1574975, Cat#101320). 5 min after Fc blocker treatment ...
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bioRxiv - Immunology 2024Quote: ... Cell pellets were resuspended in 100 µL of Fc-blocking medium (5 µg/ml Purified anti-mouse CD16/32 Antibody (Biolegend, San Diego, CA) in FACS buffer) ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the NK-92 staining panel: anti-CD107a (LAMP-1) FITC (BioLegend catalog #328606, clone H4A3), anti-CD56 (NCAM ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the PBMC staining panel for T cells: anti-CD4 FITC (BioLegend catalog #317408, clone OKT4), anti-CD26 PerCP/Cy5.5 (BioLegend catalog #356112 ...
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bioRxiv - Physiology 2024Quote: ... collected into FACS tubes (Falcon, cat no. 352052) and incubated with the following antibodies: AF700-labeled CD54 (1:100, BioLegend, cat no. 353126) and PE/Cy7-labeled CD62E (1:100 ...
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bioRxiv - Microbiology 2024Quote: ... Slides were blocked with 5% normal goat serum in PBS supplemented with 0.3% v/v Triton X-100 for 1 hr at room temperature and stained overnight at 4 °C with primary antibody (LAMP1 – Cell Signaling Technologies [CST] D2D11; CTSB – BioLegend 948001) diluted in antibody dilution buffer (PBS with 1% w/v bovine serum albumin and 0.3% v/v triton X-100) ...
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bioRxiv - Immunology 2024Quote: ... a portion of cells were stained with Ghost Dye Red 780 Live/Dead stain (Tonbo, catalog no. 13-0865-T500) and antibodies targeting CD4 (Biolegend, catalog no. 344634), FOXP3 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Cells were counted and stained with Ghost Dye Red 780 Live/Dead stain (Tonbo, catalog no. 13-0865-T500) and antibodies targeting FOXP3 (Biolegend, catalog no. 320116) and HELIOS (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... a portion of cells were stained with Ghost Dye Red 780 Live/Dead stain (Tonbo, catalog no. 13-0865-T500) and antibodies targeting CD4 (Biolegend, catalog no. 344620), CD25 (Tonbo ...
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bioRxiv - Immunology 2024Quote: ... and stained with Ghost Dye Red 780 Live/Dead stain (Tonbo, catalog no. 13-0865-T500) and antibodies targeting CD4 (Biolegend, catalog no. 344634), FOXP3 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... anti-IgD (follicular B cell marker, Alexa Fluor® 647 anti-mouse IgD antibody, 11-26c.2a, catalog #405708, Biolegend, San Diego, CA), anti-B220 (B cell marker ...
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bioRxiv - Microbiology 2024Quote: ... The bottom half of the membrane was then incubated with purified rat anti-DYKDDDDK Tag antibody (anti-FLAG tag; BioLegend #637319 clone L5) diluted to 1:5,000 in 5% milk in TBST rocking overnight at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Coverslips were washed three times with PBS before incubating with secondary antibody AF488-goat anti-rabbit (Thermo A11034, 1:1000) and AF594-conjugated mouse anti-HA (BioLegend 901511, 1:400) diluted in blocking buffer ...