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Citations for BioLegend :
551 - 600 of 641 citations for 6 Methoxy 3 methyl 1H indazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... mice were injected into the retro-orbital sinus with a total of 3 mg of PE/Cy7-CD45.2 (104, BioLegend) in 200 μl sterile DPBS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... For the HashTag library TotalSeq™-B0303 anti-mouse Hashtag 3 Antibody (Clone M1/42; 30-F11; 155835; BioLegend) was used to mark single cells of DMSO treated mice and TotalSeq™-B0304 anti-mouse Hashtag 4 Antibody (Clone M1/42 ...
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bioRxiv - Immunology 2024Quote: ... about 3-4 million CD8+ T cells were blocked with Fc receptor blocking solution (Human TruStain FcX, BioLegend, #422302) for 10 minutes on ice and stained with BEAM-T assemblies for 15 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... with the addition of either DCs (1 cell:3 Tresp) with 0.5 μg/ml purified anti-CD3 (145-2C11, Biolegend), or Dynabeads Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation (Gibco ...
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bioRxiv - Genetics 2021Quote: ... production from LPS- or OL-treated BMDM were performed using Mouse TNF-α ELISA MAX Deluxe kit and Mouse IL-6 ELISA MAX Deluxe kit (BioLegend, San Diego, CA, USA). Follow-up cytokine (IL1β ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 min) and resuspended in 100 μl of serum medium supplemented with 0.5 μl SSEA-I (BioLegend, cat. no. 125607) and 0.15 μl PE-CD61 antibodies (BioLegend ...
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bioRxiv - Neuroscience 2020Quote: ... Permeabilization was followed with blocking step by incubating the samples for 2 hr in PBS containing 3% normal goat serum (NGS, 927503, BioLegend) and 3% BSA and then stained overnight at 4°C with the primary monoclonal antibody (mouse anti-radixin ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue samples were blocked/permeabilized using 10% normal goat serum in 0.3% Triton-X in PBS for 3 hours at room temperature followed by additional avidin biotin blocking using a kit according to manufacturer’s instructions (BioLegend, 927301). Primary immuno-staining was performed using antibodies ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The suspended cells were then transferred to a fresh flask and activated in fresh RPMI-1640/10% FBS/PSG by 3:20,000 anti-CD3 (BioLegend 317303), 3:20,000 anti-CD28 (BioLegend 302913) ...
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bioRxiv - Systems Biology 2021Quote: ... deeply anesthetized mice (5% isoflurane) were retro-orbitally injected with 3 μg of anti-CD45 antibody (PerCP, clone 30-F11, Biolegend) 3 min prior to euthanasia [46,81] ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... Slides were again washed 3 times with 1x TBS and stained with FITC–anti-IgD mAb (clone 11-26c.2a; 405713, BioLegend), PE goat-anti-mouse-IgM mAb (406507 ...
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bioRxiv - Immunology 2021Quote: ... Cells were stimulated for 3 hours at 37 °C with complete IMDM medium with 1x Cell Activation Cocktail (with Brefeldin A, Biolegend). After stimulation ...
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bioRxiv - Cell Biology 2022Quote: ... BSA- and IgG-coated dynabeads for pre-clearing were prepared by mixing dynabeads slurry (40 μl per sample) with 500 μl PBS/3% BSA or 500 μl PBS with 2.5 μg mouse IgG1 (BioLegend, 400102) respectively ...
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bioRxiv - Immunology 2020Quote: Recipient mice were infused with 2-3 x 106 purified donor T cells that were labeled with 5 μM CFSE (BioLegend) as previously described63 ...
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bioRxiv - Immunology 2020Quote: ... Enriched cells were then stained for the indicated cell surface antigens (Supplementary Table 3) in conjunction with TotalSeq-A oligonucleotide-conjugated antibodies (BioLegend) to allow for barcoding and pooling of different TEC subpopulations and subsequently sorted into 4 subpopulations ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were washed again 3 × 5 minutes in PBS and incubated overnight with rat anti-phospho-TDP primary antibody (1:100, 829901, BioLegend), in a dilution buffer (0.05% Tween ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 2 was used to identify DE cells and contained 3 μl of anti-human CD5-APCeF780 antibody (clone L17F12, Biolegend), 2 μl of anti-human CD19-PECy7 antibody (clone HIB19 ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 1 was used to identify CD3+CD14+ cells and contained 3 μl of anti-human CD3-AF700 antibody (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE antibody (clone 61D3 ...
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bioRxiv - Developmental Biology 2020Quote: Cells were resuspended in 5ml CMF-PBS with 3% BSA (MilliporeSigma, A9576-50ml) and plated on anti-GD3 (Biolegend, 917701) 10cm plates (untreated plates coated overnight at 4°C with 13μg GD3 antibody in 10ml 50mM Tris-HCl pH9.5 ...
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bioRxiv - Immunology 2020Quote: ... mice were anesthetized with Ketamine/Xylazine (Patterson Veterinary) and injected intravenously with 3 μg of fluorophore-labelled anti-CD45 (30-F11, Biolegend) monoclonal antibody through the retro-orbital sinus to label intravascular leukocytes ...
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bioRxiv - Genomics 2020Quote: Cell and nuclei labelling were performed according to an adapted Biolegend cell hashing protocol (TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 3.1 Protocol, Biolegend). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were blocked in 10% BSA in PBS for 1 h at room temperature and then incubated with a MAP2 antibody diluted in 3% BSA (MAP2 chicken, 1:10000, Biolegend) for 60 min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... The MNC fraction was depleted of red blood cells by lysis steps involving 3 incubations at room temperature for 20 mins/10 mins/10 mins respectively with RBC lysis buffer (BioLegend). Tissue lymphocytes were isolated from Peyer’s patches in intestinal mucosa using laser capture microdissection and subjected to protein lysis as outlined above ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell viability was assessed by flow cytometry after 3 days of drug or vehicle treatment using Helix NP NIR viability dye (BioLegend). Dose-response curves were fitted through interpolation using GraphPad Prism v.7 nonlinear regression fit ([inhibitor] versus normalized response–variable slope).
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bioRxiv - Synthetic Biology 2021Quote: ... incubated at a concentration of 0.1 µg/mL in HF for 3 minutes at room temperature or Zombie NIRTM (BioLegend, 423106) incubated in PBS at a 1:500 dilution for 15 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit) at 1:100 dilution (for immunodepletion) or 3 μg/ml of each 4G8 and 6E10 anti-Aβ antibodies (Biolegend) (for brain-derived Aβ enrichment ...
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bioRxiv - Microbiology 2023Quote: ... for 5 minutes at 4°C and then fixed using commercial fixation buffer for 3 minutes (ref no. 420801, BioLegend). For intracellular staining of Granzyme B and Perforin ...
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bioRxiv - Immunology 2023Quote: ... Sip-T or single cell suspensions of tumor-infiltrating lymphocytes (TILs) were resuspended in PBS as 1-3×106 cells/ml and stained with fixable Zombie Aqua viability dye (BioLegend) as per manufacturer’s recommendation ...
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bioRxiv - Immunology 2023Quote: ... Panels 2 and 3 incorporated anti-Digoxygenin (RD System, #611621), anti-FITC (Standard BioTools, #3174006B) and anti-Biotin (Biolegend, #409002) metal-tagged antibodies to detect the RNAscope staining output.
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bioRxiv - Immunology 2023Quote: ... Pooled cells from n = 3 - 5 mice/cohort were stained with PE- or APC-conjugated antibodies (from either BD or BioLegend) against murine F4/80 (clone ...
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bioRxiv - Immunology 2023Quote: ... Cells were activated for 3 h in a round-bottom 96-well plate using Cell Activation Cocktail (with Brefeldin A) (BioLegend). Plates were then centrifuged at 300 x g for 5 min and the pellets resuspended in 50 µL of Cell-ID™ Cisplatin-195Pt viability reagent (Standard BioTools) ...
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bioRxiv - Immunology 2023Quote: ... 106 of the CTV labeled T cells were stimulated in vitro for 3 or 5 days in cultures containing 100ng/ml IL-15 (BioLegend) or a combination of 100ng/ml IL-7 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8+ T cells were cultured in RPMI (10% FCS) and stimulated with plate-bound mCD3 mAbs (3 µg ml-1, clone 145-2C11, Biolegend), soluble mCD28 (0.5 µg ml-1 ...
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bioRxiv - Immunology 2023Quote: ... were derived from a cell sort that included an additional cell hashing step (TotalSeq-C0251/C0252/C0253/C0254/C0255 anti-human Hashtag 1/2/3/4/5 antibodies, cat # 394661/394663/394665/394667/394669, Biolegend) prior to B cell enrichment and staining ...
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bioRxiv - Immunology 2022Quote: 3×106 cells from each tonsil donor (n=3) at each time point were stained with the Zombie Red fixable viability kit (Biolegend) for 10 min at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... protein expression (ADT) and hashtag (HTO) libraries were generated using Chromium Single Cell 3’ version 2 reagents (10x genomics and Biolegend) per manufacturers’ protocols ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were cultured alone or co-cultured with either of the B-cell populations at a ratio of 3:1 for 60 h at 37°C and 5% CO2 on anti-CD3 (2 μg/mL) (ULTRA-LEAF Biolegend) coated flat bottomed 384 CellCarrier Ultra microplates (PerkinElmer ...
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bioRxiv - Immunology 2022Quote: ... resuspended in 50 μl FACS-buffer containing the respective fluorochrome-coupled Abs (anti-TACI/CD267 PE, clone eBio8F10-3, eBioscience, anti CD138 PECy7, clone 281-2, Biolegend, anti CD19 BV510 ...
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bioRxiv - Immunology 2023Quote: ... Cells were sorted and purified cells were stained on ice for 30 min in PBS containing 1 % (w/v) BSA with hashtag antibodies (TotalSeq-B0301/B0302/B0303/B0304B0305 anti-mouse Hashtag 1/2/3/4/5, respectively, Biolegend). Cells were washed ...
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bioRxiv - Immunology 2023Quote: ... THP-1 and SKOV-3 cell lines and primary human T cells were labeled with fluorescent antibody against human CD70 or isotype control (Biolegend) to verify antigen expression as described before35.
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bioRxiv - Cell Biology 2023Quote: ... samples were stained for proliferation marker Ki-67 Alexa Fluor 647 (1:250 dilution in 3% BSA, Cat. No. 652407; BioLegend) for 1 hour and nuclei counterstain bisbenzimide (1:1000 dilution in 1X dPBS ...
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bioRxiv - Cell Biology 2023Quote: ... Single cells were pelleted and re-suspended in a staining solution of DMEM containing 1% FBS and anti-Ki-67 Alex Fluor 647 (1:250 dilution in 3% BSA, Cat. No. 652407; BioLegend) for 1 hour on ice ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Cells were centrifuged at 500× g at 4 °C and resuspended in 1/3 strength artificial seawater containing 2 μg/mL calcein AM (Enzo) and 100 nM sytox blue (BioLegend) to monitor viability ...
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bioRxiv - Genomics 2023Quote: ... Fluorophore conjugated antibodies against the following antigens were used for input and day 3 cells (All Biolegend unless otherwise noted): panel 1 ...
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bioRxiv - Cell Biology 2024Quote: ... the sperm smears were fixed for 10 min with 3.2 % paraformaldehyde and permeabilized with the Intracellular Staining Perm Wash Buffer for 3 x 5 min (421002, BioLegend, USA). Following permeabilization ...
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bioRxiv - Immunology 2024Quote: ... and APC anti-ligand (B7-H6: R&D, clone 875001; CD48: Biolegend, clone; BJ40; Nectin-2: Biolegend, clone TX31; LFA-3: Biolegend, cloneTS2/9 ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were fixed with cold methanol for 3 min at room temperature and incubated overnight at 4 °C with an anti-HA antibody (diluted 1:2000) (Biolegend,). Cells were then rinsed three times with PBS and incubated for 1 h at room temperature with an Alexa647-conjugated goat anti-mouse antibody (diluted 1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Residual red blood cells (RBC) were lysed incubating cell suspension for 3 minutes at 4°C with 1X RBC Lysis Buffer (BioLegend). Cell suspensions were washed with PBS ...
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bioRxiv - Immunology 2024Quote: ... Staining was performed by washing with 0.1% Triton-X 100 in PBS for 3 x 15 minutes then staining for CD3-PE (1:100, Biolegend, 100205) overnight at room temp ...