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Citations for BioLegend :
451 - 500 of 641 citations for 6 Methoxy 3 methyl 1H indazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... and suspended in 3 ml red blood cell lysis buffer (Biolegend, San Diego, CA, USA) for 90 s to remove the remaining red blood cells and diluted in 9 mL PBS after incubation ...
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bioRxiv - Microbiology 2023Quote: ... lysed in 1X red blood cell lysis buffer for 3 minutes (ref no. 420301, BioLegend), and passed successively through 70μm and 30μm strainers (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated with anti-SSEA-3 rat IgM antibody (1:1000, BioLegend, 330302) at 4°C for 1 h following incubation with fluorescein (FITC ...
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bioRxiv - Immunology 2024Quote: ... and 3 µg of Ultra-LEAFTM purified anti-mouse CD28 antibody (clone 37.51, Biolegend, # 102116) per well ...
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bioRxiv - Microbiology 2021Quote: ... and resuspended in RPMI with 10% FBS medium and were stimulated for 6 h with a cell activation cocktail containing PMA and ionomycin (Biolegend, # 423302) in the presence of protein transport inhibitor ...
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bioRxiv - Immunology 2020Quote: Supernatants of stimulated T cells were harvested on day 6 of culture and analyzed using the LEGENDplex™ human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Developmental Biology 2021Quote: ... Surface staining was done by adding the flow cytometry antibody cocktail outlined in Extended Data Table 6 containing F4/80 (BioLegend, 123120), CD11b (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were induced to differentiate by culturing in the absence of β-Estradiol for 6 days before cells were stained for CD11b (Biolegend) and analyzed on the LSRII flow cytometer (BD Biosciences) ...
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bioRxiv - Microbiology 2023Quote: ... and subsequently with biotinylated α- CADM1 antibody (MBL, Cat. No. CM004-6) and streptavidin-conjugated secondary antibody (BioLegend, Cat. No. 405226). Cells were resuspended in 4◦C PBS 2% FCS before cell sorting.
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bioRxiv - Physiology 2023Quote: The levels of TNF-α and IL-6 in the serum and the supernatant of the crushed muscle tissues were measured using a commercial kit (mouse TNF-α and IL-6 ELISA max standard set, BioLegend). The values of TA and GA muscles were revised to the protein concentration measured by BCA kit and expressed as pg/mg.
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bioRxiv - Cancer Biology 2023Quote: For inflammatory activation colon fibroblasts were plated in 6-wells and after 24 hours treated with 1 ng/ml murine recombinant IL-1α (Biolegend, 575002) in PBS for the indicated time periods ...
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bioRxiv - Cancer Biology 2023Quote: Plasma Ig were analysed using the LEGENDplex Mouse Immunoglobulin Isotyping panel (6-plex) by flow cytometry (Cat #740493, BioLegend, CA, USA). Plasma sample and standards was prepared using assay buffer as per the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2024Quote: ... Splenocytes from naive or tumor-bearing AST;Cre-ERT2 mice were mixed with 2×106 C57BL/6 splenocytes and incubated in RPMI-10 for 4 hours at 37°C in the presence of brefeldin A (Biolegend 420601) and TAG peptide (SAINNYAQKL [0.5 μM] ...
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bioRxiv - Immunology 2023Quote: ... CD3+ T cells were isolated from spleens of 6-to-8-week-old OT-II mice by MojoSort mouse CD3+ T cell isolation kit (Biolegend 480024). Stimulated FLS were washed with PBS and co-cultured with CD3+ T cells at a concentration of 5-10×105 cells/mL in 10% RPMI for 5 days with either the cognate OVA323-339 antigen (2μg/mL ...
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bioRxiv - Immunology 2024Quote: ... Lysis reaction was stopped by adding 10ml of PBS/2mM EDTA + 2% FBS and the resuspended solution was centrifuged 5min at 350g.Spleen was taken from 8- to 16-week-old mice and pressed in a 6 wells plate containing 2ml of red blood lysis buffer (Biolegend #420302) 2 more ml were added and then incubated 5min at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... Serum IL-6 and TNF-α concentrations were determined with enzyme-linked immunosorbent assay (ELISA) kits (Legend Max Mouse IL-6 ELISA Kit, BioLegend, and Mouse TNF Alpha Uncoated ELISA ...
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bioRxiv - Cancer Biology 2024Quote: Spleen was isolated from C57BL/6 mice and CD8 T cells were isolated using MojoSort™ Mouse CD8 T Cell Isolation Kit (Biolegend). The cells were stimulated with Dynabeads™ Mouse T-Activator CD3/CD8 kit (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... BMDCs were harvested 3 days later and stained with antibodies specific for CD11c (clone N418, BioLegend) and MHC-I (H-2Kb ...
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bioRxiv - Immunology 2021Quote: ... Live bone marrow cells were subject to FcR-receptor blocking (1:3; BioLegend; Cat. No. 101319) and viability staining (1:500 ...
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bioRxiv - Physiology 2020Quote: ... Live bone marrow cells were subject to FcR-receptor blocking (1:3; BioLegend; Cat. No. 101319) and viability staining (1:500 ...
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bioRxiv - Immunology 2021Quote: ... mice received IV injection of 3 μg of a Brilliant Violet 570-conjugated CD45 antibody (BioLegend) and sacrificed 3 min later and the fraction of IV-labeled cells was quantified.
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bioRxiv - Genetics 2020Quote: ... Cells were stimulated for 3 hours with 2.5 μg/ml of anti-CD3 (Biolegend, catalog 317315) and anti-CD28 (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... infected or stimulated cells using a LEGENDplex Human Type 1/2/3 Interferon Panel assay (BioLegend) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: Cytokine concentration in mouse plasma samples were quantified by a 3-plex LEGEND-plex assay (Biolegend) that contained capture beads and detection antibodies for CCL2 (monocyte chemoattractant protein-1 ...
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bioRxiv - Immunology 2024Quote: ... and CD3 (BD Biosciences, clone SK7, Cat No. 340663, used at 3 μl/test or BioLegend, clone OKT3 ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-Tubulin β 3 (TUJ1) antibody (1:1000, PRB-435P; Biolegend, San Diego, CA, USA), rabbit anti-PROX1 antibody (1:500 ...
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bioRxiv - Immunology 2023Quote: ... for 3 days at 37°C in the presence of 10 ng/ mL IL-12 (Biolegend).
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bioRxiv - Immunology 2024Quote: Cytokine measurements of serum were performed using LEGENDplex murine Th1/Th2 8 -plex Version 3 (BioLegend) as per kit instructions with data acquired using a Fortessa (BD ...
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bioRxiv - Immunology 2024Quote: ... B cells were stained with 3 μL FITC anti-human CD20 antibody (BioLegend, 302304, clone: 2H7), 3.5 μL Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used in this study: Mouse-anti-Tubulin beta-3 (TUBB3) antibody (BioLegend), chicken-anti-MAP2 antibody (ThermoFisher) ...
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bioRxiv - Immunology 2020Quote: ... KC and IL-6 in the cell culture supernatants and lung homogenates were determined by commercialized ELISA kit (IL-1β and KC were from R&D Systems BD, IL-6 was from Biolegend) and SAA3 in the lung homogenates and plasma were determined by ELISA kit (MilliporeSigma ...
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bioRxiv - Microbiology 2021Quote: ... Intracellular P-STAT5 staining was then performed in PBS with wash steps performed at 1800 rpm for 6 min at 4°C.The following antibody clones and fluorochromes were used: CD3 (UCHT1, Biolegend; Bv510, Bv711, FITC), CD8 (SK1 ...
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bioRxiv - Immunology 2020Quote: ... naïve CD4+ T cells were isolated from the spleens of C57BL/6 mice or IL17-GFP mice using a MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit (BioLegend cat. 480040). T cells from C57BL/6 mice were further labeled with CellTrace™ Violet (Invitrogen cat ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells were isolated from the spleens of C57BL/6 mice using the MojoSort™ Mouse CD8+ T Cell Isolation Kit (Biolegend, #480035), followed by 1X Red Blood Cell lysis buffer (eBioscience ...
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bioRxiv - Microbiology 2023Quote: Cytokine levels in the serum and spleen of mice infected with SPN were measured using ELISA MAX™ Standard Set Mouse IL-6 (BioLegend 431301) and ELISA MAX™ Standard Set Mouse IL-1β (BioLegend 432601 ...
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bioRxiv - Immunology 2023Quote: ... IL-1b, IL-6, IL-10, IL-17A, IL-12p70, GM-CSF, IL-23, IFN-b, MCP-1, IL-27; BioLegend Cat# 740446), Mouse TH Panel v4 (TNF-α ...
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bioRxiv - Immunology 2023Quote: ... IL-4, IL-2, IL-6, IL-10, IL-17A, IL-17F, IL-5, IL-9, IL-13, IL-22; BioLegend Cat# 741044) and Mouse Custom696 Panel (CXCL1(KC) ...
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bioRxiv - Bioengineering 2023Quote: ... the serum samples collected at 6 hours and 48 hours after administration were diluted 2-fold for ELISA analysis using the LEGEND MAX™ Rat IL-6 ELISA Kit with pre-coated plate (Biolegend, USA) according to the manufacturer’s recommendations with a little modification as described below ...
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bioRxiv - Immunology 2023Quote: ... IFN-α and IL-6 were detected by the Mouse Anti-Virus Response Panel (13-plex) (Cat# 740622 BioLegend, San Diego, CA) and analyzed on the NovoCyte Flow Cytometer (Agilent ...
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Most L1CAM is not associated with extracellular vesicles in human biofluids and iPSC–derived neuronsbioRxiv - Neuroscience 2024Quote: ... EBs were plated on Geltrex™-coated 6-well plates (10 EBs/well) in APEL™2 medium supplemented with VEGF (40 ng/ml) (BioLegend), SCF (50 ng/ml ...
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bioRxiv - Biochemistry 2024Quote: ... in RPMI complete medium in a 6-well plate that was pre-coated overnight at 4°C with anti-CD3 antibody (BioLegend Cat# 100340) at 100 pg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... IL-6 and IL-15 was quantified in cell supernatants using a sandwich ELISA Max Deluxe Set Human (Biolegend, San Diego, CA), according to the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2024Quote: ... we extracted the blood vessels and popliteal lymphatic vessels and hashed the samples with TotalSeq™-A0306 anti-mouse Hashtag 6 Antibody (Biolegend, 155811) and TotalSeq™-A0305 anti-mouse Hashtag 5 Antibody (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... α-CCR10-APC (clone 6588-5) and α-TIM-3-PE-Cy7 (clone F38-2E2) from BioLegend. α-ULPB2/5/6 (mouse ...
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bioRxiv - Cancer Biology 2021Quote: ... and supplemented with murine SCF (10 ng/ml) and IL-3 (10 ng/ml) (Peprotech or BioLegend). The cells were serially passaged for ∼1 month ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were blocked with PBS containing 3% BSA and stained with Alexa Fluor 647-anti-DYKDDDDK (BioLegend) at 0.5 μg/mL or with Alexa Fluor 488-streptavidin (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated with secondary antibody diluted in 3% BSA (Alexa Fluor 594 Goat anti-Rat IgG, BioLegend, #405422) in the dark for 1 hr at room temperature ...
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bioRxiv - Immunology 2023Quote: ... we used Ultra-LEAF™ purified anti-human TIM-3 antibody (10μg/ml, clone F38-2E2, Biolegend) or its respective isotype Ultra-LEAF™ purified mouse IgG1 antibody (10μg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with Alexa Fluor 488 anti-mouse/human Mac-2 (Galectin-3) antibody (125410, Biolegend) or alternatively with Brilliant Violet 421 anti-mouse/human Galectin-3 antibody (125416 ...
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bioRxiv - Microbiology 2024Quote: ... and neurons were labelled with rabbit anti-tubulin beta 3 (TuJ-1) IgG at 1:500 (Biolegend), in PBS ...