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Citations for BioLegend :
5901 - 5950 of 6089 citations for Piggybac Transposable Element Derived 3 PGBD3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... followed by flow cytometric analysis of the uptake of fluorescent OVA and the presentation of the OVA257-264 (SIINFEKL) by mouse MHC-I (H2Kb) using a PE-conjugated anti-H2Kb-SIINFEKL antibody (Biolegend; clone 25-D1.16). To further confirm the role of S100A4 in promoting antigen cross-presentation ...
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bioRxiv - Genomics 2020Quote: ... Cells were blocked with 1% BSA/PBS for 30 minutes and then incubated with antibodies for HA.11 (BioLegend MMS-101P, 1:2000) and BIP (Abcam ab21685 ...
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bioRxiv - Immunology 2021Quote: The following antibodies were used for the analysis of human synovial cells with cell sorting: APC anti-CD3 (Clone OKT3, BioLegend Cat# 317317, RRID:AB_1937213), PE anti-CD45RO (Clone UCHL1 ...
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bioRxiv - Microbiology 2021Quote: ... 20 μl cells were removed for flow analysis and remaining cells were incubated for 30 min on ice with 1 μg each of TotalSeq™-A0441 anti-mouse CD71 Antibody (Biolegend, Cat# 113824) and TotalSeq™-A0073 anti-mouse/human CD44 Antibody (Biolegend ...
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bioRxiv - Bioengineering 2021Quote: ... Anti-FLAG epitope (DYKDDDDK, catalog# 637301) and horseradish peroxidase (HRP) Donkey anti-human IgG antibody (catalog #410902) were purchased from Biolegend (San Diego, CA). The secondary antibody anti-rat IgG HRP (catalog# 7077 ...
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bioRxiv - Bioengineering 2021Quote: ... 22 and 28 before differentiation of mast cells was assessed by flow cytometry with antibody markers for CD117 APC (2B8, Biolegend, San Diego, CA) and FcεRI PE (MAR-1 ...
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bioRxiv - Microbiology 2020Quote: ... After 48h of co-infection the cells were harvested and processed as above using an anti-MHC-II FITC-conjugated antibody (Biolegend San Diego, CA). Treatment with PMA (50 ng/ml ...
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bioRxiv - Neuroscience 2020Quote: ... 0.05% Sodium Azide in PBS) and incubate overnight at 4°C with the monoclonal anti-amyloid beta antibody 6E10 (Biolegend, 803001, lot. B228658) at a concentration of 3μg/ml (Antibody buffer ...
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bioRxiv - Microbiology 2021Quote: ... incubated 15 minutes on ice and then stained at RT in the dark for 20 minutes with the following mixture of fluorescent-labeled antibodies: CD3-APC (Biolegend, San Diego, CA), CD4-BV510 (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: ... Aβ peptides were extracted from 250 µL human plasma using immunoprecipitation with anti-β-Amyloid 17-24 (4G8) and anti-β-Amyloid 1-16 antibodies (6E10, both Biolegend®) coupled to Dynabeads™ M-280 Sheep Anti-Mouse IgG magnetic beads (Thermofisher ...
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bioRxiv - Cancer Biology 2020Quote: ... coated with intercellular adhesion molecule (ICAM-1, ICAM-2) and vascular cell adhesion molecule (VCAM-1) antibodies conjugated to Alexa Fluor 680 (BioLegend, San Diego, CA). Sorted cells were cultured in Endothelial Growth Media (EGM-2 ...
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bioRxiv - Cancer Biology 2020Quote: ... We split our single cell suspension in half and stained half of the uncultured HCI010 and HCI002 cells for FACS using fluorescent labeled antibodies for human specific CD298 (Biolegend, cat. no. 341704) and mouse-specific MHC-I (eBioscience ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were stained for 30 min at +4°C with APC or PE-conjugated anti-CD133 antibodies (diluted at 1/200 or 1/400 respectively; Biolegend, San Diego, USA) or corresponding control Immunoglobulin G1 (IgG1 ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were stained for 20 minutes on ice with 1µg of BioLegend TotalSeq-A anti-human hashtag oligo antibody (BioLegend TotalSeq-A0251 to A0258). Cells were washed 3 times then pooled at equal ratios for sequencing ...
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bioRxiv - Neuroscience 2022Quote: ... Splenocytes were then filtered through a 40μM filter and stained with an antibody cocktail [Pacific Blue anti-CD45.1 (Biolegend no. 110722; 1:100 dilution), APC anti-CD45.2 (Biolegend no ...
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bioRxiv - Genomics 2022Quote: ... CD45+ tumor-infiltrating leukocytes were stained with 2.5 μg/ml of Totalseq anti-mouse Hashtag-A antibodies (A0301-A0314, BioLegend, San Diego, CA, USA) at 4°C for 25 minutes and washed thrice with Cell Staining Buffer (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... extracellular staining was done in ice-cold buffer (phenol red–free DMEM, 2% FBS) for 30 min using antibodies against CD45 (30-F11; BioLegend, dilution 1:200), CD4 (RM4-5 ...
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bioRxiv - Immunology 2022Quote: ... 2×107 cells resuspended in 0.5 ml PBS were incubated with PE-conjugated mouse anti-human CD15 antibody (BioLegend, San Diego, CA, USA) diluted 1:40 ...
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bioRxiv - Genomics 2022Quote: ... Bone marrow mononuclear cells were stained for 30 minutes on ice with the following antibodies: anti-CD19(APC/Cy7) (clone HIB19; Biolegend; Cat. num. 302217) in a 1:20 dilution ...
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bioRxiv - Immunology 2022Quote: ... CD11bhi, F4/80hi) were isolated by FACS using antibodies against CD11b (eBioscience, #11-0112, 1:1600) and F4/80 (Biolegend, #123114, 1:200), and total RNA was extracted using RNeasy Plus Mini Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed in PBS/1% BSA buffer and incubated for 30 minutes on ice with the following antibodies from Biolegend (San Diego, USA): FITC-anti-human CD38 (#303503) ...
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bioRxiv - Cell Biology 2022Quote: ... single cell suspensions of WT intestinal epithelium were stained with CytoID as detailed above and incubated with anti-mouse Hashtag antibodies (BioLegend Cat #155839, 155841,155843,155845). CytoID high and low cells were then sorted and processed separately for sequencing library generation ...
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bioRxiv - Neuroscience 2020Quote: ... and were stained at 20 μl of FACS buffer per 1×106 cells containing the first set of following antibodies (all from Biolegend, unless specified otherwise): biotinylated hematopoietic lineage antibodies against NK1.1 (clone PK136) ...
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bioRxiv - Molecular Biology 2020Quote: Proteins were resolved by SDS-PAGE and detected by Western blotting using mouse monoclonal antibodies against σ70/σA (clone name 2G10, Biolegend, cat. No. 663208), against the β subunit of RNAP (clone name 8RB13 ...
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bioRxiv - Cell Biology 2021Quote: ... Nerves were harvested at 7 days post-injury to assess vascularization and axon regeneration via antibody reactivity to mouse anti-TUJ1 for microtubules in axons (Biolegend Catalog No. 801202) and rat anti-PECAM-1 for endothelial cells within vessels (Biosciences ...
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bioRxiv - Immunology 2022Quote: ... This is followed by secondary staining for 30 min at 4°C with APC-conjugated mouse anti-His antibody (BioLegend, #362605, 1:50) or isotype control ...
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bioRxiv - Immunology 2022Quote: ... the cells were incubated for 15 minutes on ice with an Alexa Fluor 647-anti-IgG antibody (clone M1301G05, Biolegend, San Diego, CA). Control experiments were performed by skipping the incubation with TIM-4 ...
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bioRxiv - Developmental Biology 2022Quote: ... MGs for whole mount samples and cryosections were stained with a rabbit anti-keratin 14 (K14) polyclonal antibody (1:1000, 905301, BioLegend, San Diego, CA). MG whole mounts and cryosections were imaged with a Zeiss LSM880 confocal microscope ...
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bioRxiv - Biochemistry 2022Quote: ... KO clones were obtained by limiting dilution and KO was confirmed by sequencing the target sites in the genomic DNA and by flow cytometric analysis of CD59 expression [staining with mouse anti-hCD59 antibody (5H8) followed by phycoerythrin-conjugated anti-mouse IgG (Biolegend, San Diego, CA)] ...
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bioRxiv - Immunology 2020Quote: Single live cells from individual mice were isolated by flow cytometry and labeled with TotalSeq-B cell hashing antibodies (Biolegend, San Diego, CA). Labeled cells were subjected to 3’ version 3.1 transcriptome library preparation using the Chromium 10X system (10X Genomics ...
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bioRxiv - Cell Biology 2021Quote: ... IHC staining of mast cell tryptase was carried using mouse anti-human Mast Cell Tryptase Antibody (catalog # 369402, RRID:AB_2566541, BioLegend®, San Diego, CA, USA) [performed by the Northwestern University Mouse Histology and Phenotyping Core] ...
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bioRxiv - Biochemistry 2020Quote: ... or biotinylated anti-repebody antibody (1 μg/mL, AbClon, Seoul, Korea) and HRP-conjugated streptavidin (1:1000 dilution, BioLegend, San Diego, CA, USA). For trastuzumab (Herceptin) ...
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bioRxiv - Immunology 2021Quote: ... cellular suspensions isolated from bone marrow were stained at 4°C in the dark in FACS buffer with lineage markers including phycoerythrin (PE) anti–mouse antibodies directed against directed against B220 (BioLegend, clone RA3–6B2), Cd11b (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... was used to detect T cells and the following anti-human antibodies were used to identify T cell subsets: anti-CD137-PeCY7 (BioLegend Cat# 309818, RRID:AB_2207741), anti-CD103-BV605 (BioLegend Cat# 350218 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated for 30 min at 4°C with pre-conjugated fluorescent labeled antibodies with the following combinations: CD45 (BioLegend, 368532 (PE/Cyanine7)) ...
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bioRxiv - Neuroscience 2020Quote: ... anti-neurofilament to a phosphorylated heavy chain neurofilament (NF-H; mouse anti-human monoclonal antibody SMI-34, dilution 1:100, epitope retrieval time 10 min, Biolegend 835503; Biolegend, San Diego, CA). Hemosiderin deposition was evaluated on a section with only counterstain ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated with Fc-blocking anti-CD16/32 anti-bodies followed by cell hashing antibody (1 μg/ml, BioLegend Cell Hashing A) (Stoeckius et al. ...
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bioRxiv - Immunology 2020Quote: Mice were bled from the submandibular vein and the erythrocytes in the blood were lysed with ACK lysis buffer and stained with the following antibodies: mouse-CD45-PECy7 (BioLegend; clone 30-F11), human-CD45-Pacific Orange (Thermo Fisher ...
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bioRxiv - Genomics 2021Quote: ... Sections were incubated with the following primary antibodies at the indicated dilution in blocking buffer overnight at 4°C: NeuN (BioLegend 1B7, cat #834502), p65 (BioLegend Poly6226 ...
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bioRxiv - Immunology 2021Quote: ... Cells were recovered via centrifugation and stained for flow cytometric analysis as described above using the following fluorochrome-conjugate monoclonal antibodies: TIM4 (BioLegend, clone: F31-5G3), CD45.1 (BioLegend ...
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bioRxiv - Molecular Biology 2023Quote: Populations of NDFs or HT1080 cells expanded after electroporation with approximately 50% of 53-positive and p53-negative cells were treated with 1 μM CBL0137 or 500 μg/ml bleomycin for 24 hr and then labeled with hashtag antibody oligonucleotide conjugate (HOT) from Biolegend (San Diego, CA) as follows ...
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bioRxiv - Neuroscience 2023Quote: ... The following monoclonal antibodies were used for flow cytometry analyses at a dilution of 1/700: anti-CD45 (BioLegend, clone 30-F11, 103147), anti-CD11b (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... in 6-well plates in a total volume of 3mL of medium and collected after exposure/drug treatment via trypsin and trypsin-neutralizer and according to manufacturer protocol using FITC anti-BrdU antibody (Biolegend, San Diego, CA). Flow cytometric measurements were performed on a FACS Fortessa (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... All cytokine antibodies were used at 1:100 dilution in 1x BD Perm/Wash buffer (anti-IFN-γ (APC/Cyanine7 or PE, Biolegend, #505849, #505808) and anti-TNF-ɑ (BV711 Biolegend ...
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bioRxiv - Neuroscience 2023Quote: ... Extracellular deposits of Aβ peptide were immunostained with the mouse anti-β-amyloid 17-24 antibody (1:5000, clone 4G8, BioLegend, Koblenz, Germany). Alpha-synuclein pathology was detected with the anti-syn-1 antibody (1:2000 ...
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bioRxiv - Immunology 2023Quote: Purified and biotinylated IL-6 (504502 and 504601, respectively) and IL-10 (505002 and 504906, respectively) antibodies were purchased from Biolegend (San Diego, CA). Purified antibodies were used to coat an ELISA plate ...
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bioRxiv - Cancer Biology 2024Quote: ... Aliquots were thawed and incubated with the panel of antibodies following the blockade of non-specific binding of the immunoglobulin to the Fc receptors by TruStain FcX (BioLegend, San Diego, CA). LIVE/DEAD Fixable Near-IR Dead Cell Stain dye (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... blood cells were lysed using VersaLyse buffer and blood and CSF cells were stained using the following anti-human antibodies (Biolegend; clone names indicated): CD3 (UCHT1) ...
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bioRxiv - Molecular Biology 2024Quote: ... according to manufacturer’s recommendations and stained for 1 hour at 4°C with the following antibodies: NGFR (345110; 1:600, BioLegend, San Diego, CA, USA), α-tubulin (NB100-69AF405 ...
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bioRxiv - Molecular Biology 2023Quote: ... and spleen were counted on a Pentra ES60 Haematology Analyser (Horiba) and stained with anti-mouse antibodies against the following antigens as appropriate (all from BioLegend unless otherwise stated) in FACS buffer (PBS with 2% BSA and 0.025% sodium azide) ...