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Citations for BioLegend :
5601 - 5650 of 10000+ citations for Goat anti mouse HRP secondary antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... were isolated ex vivo from mouse peritoneal cavity by intraperitoneal lavage followed by magnetic bead-based enrichment for c-Kit+ cells using anti-CD117 (c-Kit)-APC antibody (Biolegend) and APC microbeads (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2024Quote: ... mesothelioma cell lines were further pre-treated with anti-MHC class I antibody (10 μg/ml) (Clone W6/32, Biolegend) for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... NK cells were analyzed by flow cytometry for expression of NKG2A using above mentioned protocol but with anti-human NKG2A PECy5 antibody (Biolegend).
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bioRxiv - Cell Biology 2023Quote: ... Cell surface expression level of syndecan-1 was evaluated by incubating cells with anti-syndecan-1 antibody (10 µg/ml, B280312, Biolegend) for 1h at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... 100 uL/well) were restimulated with coated anti-CD3 antibody (1 ug/mL, 100 uL/well, 4 °C overnight, Clone: OKT3, Biolegend) and soluble anti-CD28 antibody (2 ug/mL ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were precooled for 20 min at 4 °C and then incubated with HDL-SOG for 40 min at 4 °C with 50 μg HDL per ml PBS or with anti-SCRB1 antibody-SOG (BioLegend, 363201 ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit) at 1:100 dilution (for immunodepletion) or 3 μg/ml of each 4G8 and 6E10 anti-Aβ antibodies (Biolegend) (for brain-derived Aβ enrichment ...
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bioRxiv - Immunology 2023Quote: CAR-NK92 cells and NK92 cells were suspended with fresh culture medium supplied with 2 nM monensin and anti-CD107a-PE antibody (BioLegend) (1:1000 dilution) ...
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bioRxiv - Biochemistry 2023Quote: ... The NMM/DMSO was incubated for 40 hours at 4°C while the primary antibody anti-tubulin beta- III (#802001, BioLegend), diluted at 1:2000 in 0.15% Triton-X in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... When staining for SLAM markers the same lineage cocktail was used (FITC) with the addition of the following antibodies: anti-CD48 (FITC, clone HM48-1, BioLegend), anti-CD41 (FITC ...
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bioRxiv - Cancer Biology 2023Quote: ... and then incubated for one hour in PE anti-human CD71 Antibody in the dark at 4°C (1:100; Biolegend). Cells were washed twice with PBS and then and then analysed using a flow cytometer (FACsFortessa ...
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bioRxiv - Microbiology 2023Quote: ... for 1 hour at 25°C and then transferred to TBST supplemented with purified anti-HA antibody clone 16B12 (Biolegend) at a 1:2000 dilution for 1h at 25°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with the following fluorochrome-conjugated antibodies: APC/Fire™ 750 anti-human CD324 (E-Cadherin) (Biolegend 324122), PE anti-human CD326 (EpCAM ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with cold PBS and then stained with a cocktail of fluorescently labeled antibodies including: anti-human CD3-PE-Cy7 (Clone: OKT3, Biolegend), Ghost viability dye (Tonbo Biosciences),TCR a/b-BV421 (Clone ...
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bioRxiv - Synthetic Biology 2023Quote: ... OD600 of induced cultures was taken and used to determine approximate volumes of culture to stain two million cells with fluorescently labeled antibodies (anti-FLAG PE, Biolegend, cat# 637309 ...
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bioRxiv - Immunology 2023Quote: ... We stably expressed these plasmids in OCI-Ly8 cells and used fluorescence activated cell sorting (FACS) to enrich for cells that stained negative with PE anti-human CD20 antibodies (clone 2H7, Biolegend). This sorted CD20-negative cell population were the OCI-Ly8 CD20KO cells.
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bioRxiv - Cancer Biology 2023Quote: ... Jurkat transfectants were activated for 5 minutes at 37°C in serum-free RPMI-1640-sodium bicarbonate by crosslinking the anti-CD3 antibody (1μg/ml, clone UCHT1, BioLegend #300402) with secondary anti-mouse antibodies for immunoblot analysis of GSK-3 α/β and Akt phosphorylation.
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bioRxiv - Genomics 2022Quote: ... The custom oligos were synthesized by Integrated DNA Technologies and conjugated to anti-Nuclear Pore Complex Proteins antibody (Clone Mab414, BioLegend) by BioLegend ...
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bioRxiv - Immunology 2023Quote: ... cells were washed three times with FACS buffer (PBS+0.5% BSA+2% FBS+0.1% sodium azide) and stained with the following antibodies for 30 minutes: anti-CD4 PerCP/Cy5.5 (BioLegend Cat: 100540), anti-CD8a Brilliant Violet 510 (BioLegend Cat ...
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bioRxiv - Genomics 2023Quote: ... The cells were then incubated on ice for another 30 min after adding 0.5 μl of biotin anti-human CD15 antibody (BioLegend, 301913). After immunostaining ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies used to distinguish the different cell populations included Brilliant violet 421 anti-human CD19 (Biolegend, San Diego, CA, USA), PE anti-human CD45 (Biolegend) ...
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bioRxiv - Immunology 2023Quote: ... Lung resident cells were distinguished from those in the vasculature by exclusion from staining with fluorescently labeled anti-CD45 antibody (PerCP, clone 30F-11, BioLegend) that was injected retro-orbitally into each mouse approximately 3 minutes prior to euthanasia65 ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated for 30 minutes on ice with the following antibody: anti-CD44 APC/Fire 750 (1:100, Biolegend, 103061), anti-CD24 PE (1:20 ...
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bioRxiv - Molecular Biology 2023Quote: ... The percentage of purified CD34+ cell was analysed by flow cytometry staining with anti-human CD34 PE antibody (BioLegend, UK). The cells were cultured in StemSpan SFEM II medium (StemCell Technologies ...
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bioRxiv - Genetics 2023Quote: ... and 72 h by immunofluorescent staining with a PE-conjugated anti-human GPA primary antibody (BioLegend, San Diego, CA, USA) using flow cytometric analysis.
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bioRxiv - Immunology 2023Quote: ... thawed PBMCs were FACS-sorted to isolate CD8+ CD4- CD56- cells (using BV785-conjugated anti-CD8 antibody, clone SK1 (Biolegend); PerCP-Cy5.5-conjugated anti-CD4 antibody ...
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bioRxiv - Immunology 2023Quote: ... For comparison of resident vs non-resident populations tonsil samples were divided into CD69+ and CD69- subsamples using biotinylated anti-human CD69 antibody (FN40, Biolegend), and separation was performed using anti-Biotin Microbeads (Milenyi ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were injected intraperitoneally with three hundred micrograms of Ultra-LEAF purified anti-Asialo GM1 rabbit polyclonal antibody (clone Poly21460, BioLegend) every four days for the duration of the experiment ...
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bioRxiv - Neuroscience 2024Quote: ... This process was repeated once and then cells were resuspended in the antibody solution containing APC anti-rat CD45RA (Cat# 202313, Biolegend) and FITC anti-rat CD3 (Cat# 201403 ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated for 30 min at 4°C with 1 µL of PE anti-human FasL antibody (clone NOK-1) from BioLegend. Nonspecific background signals were measured by incubating cells with the appropriate isotype-matched antibody ...
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bioRxiv - Cancer Biology 2024Quote: Single cell suspensions from tumors were washed with HBSS containing 2% FBS and 1 mM EDTA and stained with the following antibody cocktails for detecting lymphoid populations: anti-CD45-PerCP/Cy5.5 (BioLegend 103132), anti-NK1.1-FITC (BioLegend 108706) ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then stained with Brilliant Violet (BV) 421TM anti-human CD138 (Syndecan-1) antibody (BioLegend catalog #356516, clone MI15), FITC anti-human HLA-A ...
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bioRxiv - Microbiology 2024Quote: ... and used to transduce K562 cells as described below in “Lentiviral production and transduction.” Transduced cells were stained using anti-DCSIGN antibody (Cat #330105, Biolegend) and cell populations highly expressing both DCSIGN and BFP were bulk sorted (Sony MA900).
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bioRxiv - Immunology 2024Quote: ... LAD2 cells were washed and incubated with the reaction products or serum-purified C3 or C3a (100 nM) along with a PE-conjugated anti-LAMP1 antibody (H4A3, Biolegend) for 1 h at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... Sections were saturated with BSA 2% PBS and then incubated overnight with an APC-conjugated anti-CD45 antibody (clone 30F11, Biolegend) at 4°C ...
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bioRxiv - Immunology 2024Quote: ... and 5 µg/mL of soluble Ultra-LEAF™ purified anti-human CD28 monoclonal antibody (clone 28.2, BioLegend, cat. 302933) in R10 medium [RPMI-1640 medium supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Immunology 2024Quote: ... cells from each well were washed thrice with PBS and stained with APC/Cyanine7 anti-human CD14 Antibody (BioLegend, #123317) to assess THP-1 cell phagocytosis of HER2-positive tumor cells ...
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bioRxiv - Immunology 2024Quote: ... It exhibits monocyte morphology and was originally derived from malignant cells obtained from the pleural effusion from a patient with histiocytic lymphoma.77 LFA-1 (CD11a/CD18) was assessed with a FITC anti-human antibody (clone m24, Biolegend), and MAC-1 (CD11b/CD18 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were resuspended in 100 µL of FACS Buffer and stained for 15 minutes at room temperature with an antibody cocktail containing anti-CD71 (1:50; PerCP/Cyanine 5.5; BioLegend, 334113), anti-CD235a (1:500 ...
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bioRxiv - Immunology 2024Quote: ... splenocyte and microglial cell Fc-receptors were blocked using a purified anti-CD16/CD32 antibody (1:200, clone 93, BioLegend) for 20 minutes at 4°C ...
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bioRxiv - Immunology 2024Quote: White blood cell and microglial cell Fc-receptors were blocked using a purified anti-CD16/CD32 antibody (1:200, clone 93, BioLegend) for 20 min at 4°C ...
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bioRxiv - Immunology 2024Quote: ... the cells were washed again and stained a second time with a Pacific-Blue-conjugated anti-CD3 antibody (clone UCHT1, Biolegend) and a LIVE/DEAD-UV dye (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... cells were centrifuged and then resuspended in FACS buffer containing an antibody cocktail consistent of anti-CD11b AF488 (101217; Biolegend), anti-CD45 APC/Cy7 (157618 ...
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bioRxiv - Pathology 2024Quote: PFA-fixed tissue sections were blocked with normal donkey serum (10% vol/vol) and incubated at 4 °C overnight with primary antibodies anti-F4/80 (123102, BioLegend), anti-Col1a1 (NB600-408 ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were fixed with cold methanol for 3 min at room temperature and incubated overnight at 4 °C with an anti-HA antibody (diluted 1:2000) (Biolegend,). Cells were then rinsed three times with PBS and incubated for 1 h at room temperature with an Alexa647-conjugated goat anti-mouse antibody (diluted 1:1000 ...
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bioRxiv - Immunology 2024Quote: Naïve CD4+ T cells isolated from WT mice spleen and LN were treated with or without 10 µg/ml anti-β2 integrin non-function blocking antibody (BioLegend) for 1 hour and added onto 96 well plate coated with anti-CD3/CD28 along with either 2.5µg/ml of rLum or rBgn or rDcn or 1µg/ml of ICAM-1 for 30 minutes to check phospho-Akt-1 expression or 6 hours to check activation CD69 expression or 72 hours to check proliferation (CD4+ T cells were pre-labeled with CFSE for proliferation assay) ...
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bioRxiv - Immunology 2024Quote: ... Isolation purity was verified by flow cytometry using antibodies against neutrophil surface markers anti-human CD11b (Alexa Fluor 594) (Biolegend) and anti-human CD15 (SSEA-1)(Alexa Fluor 488 ...
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bioRxiv - Immunology 2024Quote: ... Then cells were incubated in the dark for 20 minutes with 50µL of the antibody mixture: 1:50 each of anti-CD3-AF488 (BioLegend 100210), anti-CD4-BV510 (BioLegend 100559) ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were washed with PBS containing 2 mM EDTA and stained with antibodies against CD59 (1:100, anti-human CD59 PE, Biolegend) and C3c (1:25 ...
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bioRxiv - Bioengineering 2024Quote: ... Surface staining was carried out using a fluorochrome-labeled PE-conjugated anti-human CD11a (HI111) monoclonal antibody obtained from BioLegend.