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Citations for BioLegend :
5401 - 5450 of 10000+ citations for Goat anti mouse HRP secondary antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Engineered T-cells were stained with increasing concentrations of AF647-conjugated anti-human TCR α/β antibody (IP26: Biolegend), HLA.A2/9V-AF647 or CD19-AF647 to determine the concentration of the staining agent leading to label saturation for CMV-specific RA14 TCR T-cells ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated with the L5 clone of Alexa Fluor® 594 anti-Flag antibody (BIOLEGEND INC, 637314), diluted 1:200 in PBS buffer containing 2% BSA ...
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bioRxiv - Immunology 2022Quote: ... enriched single-cell suspensions from tumor lysates were labelled with biotinylated anti-CD11b antibody (BioLegend, San Diego, CA, USA) and separated on MACS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... Slides were then blocked and incubated overnight at 4°C with anti-Lysozyme antibody diluted 1:400 (Biolegend, #860001). Slides were washed and incubated with anti-rabbit Alexa Fluor 555 diluted 1:500 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... IP of HA-positive ribosomes was performed by incubating with Anti-HA.11 Epitope Tag Antibody (Biolegend, Cat# 901515) for 4 hours in a microcentrifuge tube on a microtube rotator kept at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... At the end of the incubation cells were stained with PE anti-CD8 antibody (clone RPA-T8, Biolegend #301008) and the degranulation extent was measured among the GFP+ population as the percentage of CD8+ T cells expressing LAMP1 on their surface by flow cytometry analysis.
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bioRxiv - Cancer Biology 2023Quote: ... were coated with 50 µL per well of Purified anti-human IFNγ Antibody (clone MD-1; #507502, BioLegend, USA) diluted 1:200 in 1X PBS (#5460-0023 ...
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bioRxiv - Molecular Biology 2023Quote: ... Penicillin-Streptomycin and IL-2 (100 IU/ml) and activated for 24 h in the presence of plate-bound anti-CD3 antibody (2.5 µg/mL; BioLegend) and soluble anti-CD28 antibody (2.5 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in the dark for 15 min at 4°C with fluorochrome-conjugated antibodies in PBS containing anti-CD16/CD32 (2.4G2; BioXcell or BioLegend). Cells were washed and ...
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bioRxiv - Immunology 2023Quote: ... Primary and conjugated antibodies used in this study were: Armenian hamster anti-CD11c pacific blue (Biolegend, 1:100, #117321), goat anti-HIF-1α (R&D Systems ...
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bioRxiv - Bioengineering 2023Quote: ... The 5x diluted streptavidin-coated nanovial suspension was then incubated with an equal volume of 71.5 μg/mL of biotinylated anti-human VEGF antibody (Biolegend) for 30 minutes at room temperature on a tube rotator and washed ...
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bioRxiv - Bioengineering 2023Quote: ... This suspension was incubated with an equal volume of 71.5 μg/mL of oligo-barcoded anti-VEGF-A detection antibody (Biolegend) for 30 minutes at 37°C with gentle vortexing every 10 minutes before washing excess antibody with one high-dilution wash (>100x nanovial volume) ...
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bioRxiv - Microbiology 2023Quote: ... for 1h at 25°C and then transferred to TBST supplemented with purified anti-HA antibody clone 16B12 (Biolegend) at a 1:2000 dilution for 1h at 25°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then fixed in 3.7% formaldehyde for 6 min and PM HA-GLUT4-GFP detected by an anti-HA antibody (901503, BioLegend). HA staining was visualized by Cy3-goat anti-mouse secondary antibody (115-165-062 ...
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bioRxiv - Cell Biology 2023Quote: ... The slides were incubated with a primary rabbit anti-MMP2 antibody (cat# 87809, BioLegend, San Diego, CA; 1:100) overnight ...
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bioRxiv - Immunology 2023Quote: ... Each B cell sample was split into two aliquots of at least 500,000 cells for labeling with different TotalSeqC anti-human cell hashing antibodies (BioLegend). Cells at a concentration of 5 million/ml were first incubated with human Fc block (BD Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... the Rpb3 level was determined as a loading control by using an anti-Rpb3 monoclonal antibody (clone 1Y26; Biolegend). ECL reagents were used for protein detection ...
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bioRxiv - Cancer Biology 2023Quote: ... C57BL/6J mice bearing orthotopic KPC tumors were treated with 10 μg g−1 anti-TNF-α antibody (BioLegend) in combination with 100 mg/kg gemcitabine (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed with ice cold PBS and surface stain with primary anti-PD-L1 (1:50) antibody (BioLegend) on ice for 90 min ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were labelled with rat-anti-Prominin-1 antibody (Brilliant Violet 421-conjugated; BioLegend, #141213, Lot# B255870, 1:1000) and Alexa Fluor 647-conjugated recombinant human EGF (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... were given by tail-vein intravenous (i.v.) injection in 200 μL of PBS with 1 μg/mL of anti-CD3 antibody (OKT3, Biolegend). Monthly post HPC transplant ...
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bioRxiv - Bioengineering 2024Quote: ... Cells from each sample were labeled with TotalSeq™-B0251-0254 anti-human Hashtag Antibodies (clone LNH-94, Biolegend, RRID ...
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bioRxiv - Immunology 2024Quote: ... 500ul of fresh cell culture media for each respective cell type were combined with 10 µl of 0.5ug/µl purified anti-human ICAM-1 blocking antibody (Clone HA58, BioLegend), added to the wells ...
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bioRxiv - Immunology 2024Quote: ... and mesenteric lymph nodes were first treated with Fc receptor block (anti-CD16/CD32 clone 2.4G2) and then stained using the following fluorochrome-conjugated antibodies purchased from Biolegend, eBioscience ...
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bioRxiv - Immunology 2024Quote: ... they were stained with 1 uL each of the following TotalSeqC surface antibodies: anti-human IgM (BioLegend, Cat#314547), anti-human IgD (BioLegend ...
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bioRxiv - Cell Biology 2024Quote: ... The sliced sections were incubated with an anti-HA monoclonal antibody (1:1000; BioLegend, San Diego, CA, USA, #901514), followed by anti-mouse Alex Fluor 488-conjugated immunoglobulin (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... P75 and PDGFRβ primary antibody were revealed with the anti-rabbit phycoerythrin fluorochrome-conjugated (PE, poly4064, BioLegend, 406408, RRID:AB_10643424) and the anti-rat Alexafluor 488 fluorochrome-conjugated antibodies (AF488 ...
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bioRxiv - Immunology 2024Quote: ... followed by another wash with PBS and staining with fluorescently conjugated monoclonal antibodies against APC conjugated anti-human CD56 (clone 5.1H11, Biolegend) for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with recombinant IL-2 (R&D Systemts) at 10ng/mL and 4µg/mL of anti-CD28 antibody (Biolegend), plated in 24-well plates coated with 2µg/mL of anti-CD3 antibody (Biolegend) ...
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bioRxiv - Neuroscience 2024Quote: ... followed by an overnight incubation with the second set of primary antibodies [1/100 anti-CD9-biotin (312112, Biolegend) and anti-P2RY12)] ...
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bioRxiv - Microbiology 2024Quote: ... and PerCP-Cy5.5-conjugated (non-Mtb experiments) or APC-conjugated (Mtb experiments) anti-HLA-DR antibody (BioLegend, clone L243) for 20 min ...
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bioRxiv - Immunology 2024Quote: ... IL-10 production was assessed also following 30 hours stimulation with immobilized anti-CD3 antibodies (5µg/ml; Biolegend, U.S.A.), supplemented with 10 ng/mL recombinant human IL-2 (rhIL-2 ...
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bioRxiv - Immunology 2024Quote: ... Cells from an individual condition (adjuvant or LM) were stained with an oligo-tagged anti-CD45 “hashtag” antibody (Biolegend). About 20,000 cells per were then loaded into Single Cell A chips (10x Genomics ...
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bioRxiv - Cancer Biology 2024Quote: ... Pluripotency marker expression was assessed every 10 passages by flow cytometry (PE anti-human SSEA-4 antibody, BioLegend, #330405) or RT-qPCR for DNMT3B ...
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bioRxiv - Cancer Biology 2024Quote: ... the stained samples were then optionally incubated and stained with Alexa Fluor® 700 anti-human CD45 Antibody (BioLegend). Post staining ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed in 2% FBS 2 mM EDTA-PBS and surface stained with the following anti-human antibodies: CD45 (PerCP/Cyanine5.5, 2D1, Biolegend), CD137 (BV650 ...
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bioRxiv - Immunology 2024Quote: ... all cells were stained with the following antibodies: anti-CD3-peridinin chlorophyll (PerCP)-Cyanine5.5 (Cy5.5) (BioLegend, Amsterdam, The Netherlands), Anti-CD4-Brilliant Violet (BV ...
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bioRxiv - Immunology 2024Quote: ... or 96-well plates precoated overnight at 4°C with 2 µg/ml anti-CD3ε antibody (145-2C11, BioLegend). For Th0 and Th17 differentiation ...
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bioRxiv - Bioengineering 2024Quote: ... The bound TCE were then detected via flow cytometry using an anti-His-tag APC antibody (cat. # 362605, BioLegend).
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bioRxiv - Bioengineering 2024Quote: ... samples were washed twice with flow buffer (HBSS with 0.25% BSA) and stained with an Alexa Fluor 647-conjugated anti-human CD8 antibody (BioLegend) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were stained with Pacific Blue™ (PB)-conjugated anti-CD45 antibody (#103126, BioLegend, CA, US; dilution 1:200), PB-conjugated anti-CD31 antibody (#102422 ...
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bioRxiv - Bioengineering 2024Quote: ... CD8+ T cells were stained overnight at 4°C using Alexa Fluor 647-conjugated anti-human CD8 antibody (BioLegend). Confocal imaging was performed using an inverted laser scanning confocal microscope ...
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bioRxiv - Bioengineering 2024Quote: ... streptavidin-conjugated MBs were washed once by centrifugation and incubated with 6 µg biotin anti-human αvβ3 antibody (304412, BioLegend) on ice for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... appropriate concentrations of specific antibodies were added for 30C:min at 4°C with 1µg/ml anti-CD31-AlexaFluor 488 (Biolegend, 303110), 1:800 anti-CD45-BUV805 (BD Biosciences) ...
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bioRxiv - Cancer Biology 2024Quote: ... The complexes formed by the beads-bound EVs were then stained with anti-CD81 APC antibody (Biolegend, clone 5A6) and signal was detected using Cytoflex flow cytometry (Beckman Coulter) ...
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bioRxiv - Immunology 2023Quote: ... 4 % goat serum and 1:300-diluted TruStain FcX™ (Biolegend) for 1 h at RT in a humidified dark chamber ...
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bioRxiv - Neuroscience 2022Quote: ... Antibodies (BioLegend) used were as follows:
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bioRxiv - Immunology 2021Quote: ... and then stained with the fluorochrome-labeled anti-mouse CD3/CD4/CD8/CD44/CD62L mAbs (all purchased from Biolegend except CD44 from BD). Cells were then treated with Cytofix/Cytoperm (BD ...
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bioRxiv - Bioengineering 2021Quote: ... 1×106 P14 or pmel splenocytes were co-stained with Db-GP33 liposomes (10 μg/ml) and anti-mouse CD8-PE (Biolegend, Clone: 53-6.7) for 30 min on ice ...
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bioRxiv - Bioengineering 2021Quote: P14 CD8+ T cells were isolated as described above and incubated with DiO-labeled Db-GP33 liposomes and anti-mouse CD8 (Biolegend, Clone: 53-6.7) at 4°C or 37°C for 30 min ...