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Citations for BioLegend :
5351 - 5368 of 5368 citations for Mouse Relaxin 3 RLN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: The metabolic activity of HDF cells cultured exposed to the films was analysed using a resazurin assay (Deep Blue Cell Viability Kit (BioLegend, San Diego, USA)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then stained for viability using Zombie NIR™ fixable viability kit for 15 min at room temperature (Cat # BioLegend, CA, USA), followed by fluorescently labeled high- or low-molecular weight HA (2.5mg/ml in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... naïve CD4 and CD8 T-cells were isolated from syngeneic cryopreserved PBMC aliquots using the MojoSort magnetic negative selection kits (BioLegend, 480041 and 480045). Naïve T-cells were incubated in complete medium supplemented with 10 IU/mL recombinant human IL-2 (R&D Systems ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Determination of the cytokine levels in the supernatants was carried out using commercial ELISA kits for human IL-6 and TNF (BioLegend, San Diego, US). ELISA was carried out according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: Cytokine concentrations in supernatants harvested from CAR T cell: target cell co-cultures were determined by ELISA utilizing IFNγ and IL2 Deluxe kits (Biolegend; 431806 and 430106) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... were determined using bead based immunoassays (CBA kit, Cat. 560484, BD PharMingen, San Diego, CA, USA; and LEGENDplex, Cat. 740003, BioLegend, San Diego, CA, USA). Log-transformed data were used to construct standard curves fitted to 10 discrete points using a 4-parameter logistic model ...
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bioRxiv - Cancer Biology 2021Quote: ... Further preparation for FACS measurement was following the protocol of the BioLegend APC Annexin V Apoptosis Detection Kit and DNA-staining with DAPI Reagent (25 μg/mL) (Biolegend, San Diego, CA, USA). 20 000 cells were assayed using a flow cytometer FACSCantoII (Becton Dickinson ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were resuspended in PBS with 2% FBS containing anti-mouse CD16/CD32 antibody (table 1) and 1:1000 Fixable Viability Dye eFluor™ 506 (eBioscience, Cat#65-0866) or Zombie UV™ Fixable Viability Kit (BioLegend, Cat#423108). Staining with primary fluorophore-conjugated antibodies directed against cell surface markers (Additional file 4 ...
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bioRxiv - Immunology 2024Quote: ... Single cell suspensions were labelled with antibodies against cell surface antigens as described above and then prepared using a Cyto-Fast Fix/Perm kit (BioLegend, San Diego, CA, US), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... cells were washed with DPBS and harvested with trypsin-EDTA and the viability analyzed by flow cytometry using the Apoptosis Detection Kit with propidium iodide (PI, BioLegend, San Diego, CA, USA) in a Cytoflex flow cytometer (Beckman Coulter ...
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bioRxiv - Cancer Biology 2023Quote: Flow cytometry viability staining was conducted by suspending murine spleen and tumor single cell suspensions in Zombie Violet fixable viability kit (Cat # 423114, BioLegend, San Diego, CA, USA) according to manufacturer instructions for 30 minutes at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... Transduced T cells were positively selected with a PE selection kit (EasySep, ref: 17684) and an anti-HA PE-conjugated antibody (Biolegend, ref: 901518, clone: 16B12) at a 1:50 dilution to enrich transduced T cells ...
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bioRxiv - Immunology 2023Quote: Plasma and culture supernatants were collected and frozen at -80°C until cytokines measurement by commercial ELISA kits (human IL-1ß and IL-18 R&D Systems; human TNF-α and IFN-γ, Biolegend).
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bioRxiv - Cell Biology 2020Quote: ... Brilliant Violet 421 anti-human CD45 and CD31 combined with a panel of cell-surface proteins targeting 332 PE-conjugated monoclonal antibodies (Biolegend, LEGENDScreen Human PE Kit, 700007) and 10 isotype controls (Biolegend ...
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bioRxiv - Genomics 2023Quote: ... Test wells and the Zombie-only control wells were incubated with Zombie Violet Fixable Dye diluted in PBS (Zombie Violet™ Fixable Viability Kit (423113, BioLegend, San Diego, CA, USA) for 15 min at 4°C in the dark ...
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bioRxiv - Bioengineering 2024Quote: ... supernatant solutions were collected for assessment of secreted tumor necrosis factor-α (TNF-α) content using an enzyme-linked immune sorbent assay (ELISA) kit (Biolegend, San Diego, CA). Cells were washed with PBS to delaminate them from the bottom of the non-treated 24-well plates after which a second wash with PBS was performed to ensure the collection of all cells ...
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bioRxiv - Cancer Biology 2022Quote: ... and double knockdown cells were treated with the IC50 value of cisplatin and viability was assessed via Annexin V and propidium iodide staining by flow cytometry (Biolegend APC Annexin V Apoptosis Detection Kit).
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bioRxiv - Immunology 2024Quote: ... cells were centrifuged at 900 x g for 5 minutes at room temperature and stained with Zombie Aqua Fixable viability kit (Biolegend, 1:1000 diluted in PBS, 100µL) for 20 minutes at room temperature in the dark to enable exclusion of dead cells ...