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Citations for BioLegend :
5151 - 5200 of 5368 citations for Mouse Relaxin 3 RLN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... cells were stained with the appropriate surface markers [dead cell marker-Zombie Aqua™ Fixable Viability Kit (Biolegend)] and CD8 (APC conjugated ...
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bioRxiv - Immunology 2021Quote: ... In order to measure secreted IL-1β we used the commercial Human IL-1β ELISA MAX kit (Biolegend), according to the manufacturer’s instruction.
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bioRxiv - Immunology 2020Quote: ... Cell death was analysed 18h later by FITC Annexin V/PI Apoptosis Detection Kit with PI (640914; Biolegend).
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bioRxiv - Immunology 2021Quote: ... the isolated B cells were incubated with 1 μl Zombie UV Fixable Viability kit (Biolegend, cat. no. 423107) per 1 ml cell suspension for 30 min on ice ...
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bioRxiv - Immunology 2022Quote: ... The concentration of CXCL10 in the media was determined using a Human CXCL10 ELISA MAX kit (BioLegend 439904).
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bioRxiv - Immunology 2022Quote: CD4+ T cells (naïve or total) were enriched with naïve or total CD4+ T cell negative enrichment kits (Stemcell, Biolegend) from the spleens of female ...
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bioRxiv - Systems Biology 2023Quote: ... Dead cells were stained with Fixable Viability Dye eFluor 780 (eBioscience) or Zombie Violet Fixable Viability Kit (BioLegend). Cells were stained with the following fluorochrome-conjugated anti-human Abs ...
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bioRxiv - Neuroscience 2024Quote: Cell suspensions were first stained for viability using Zombie NIR™ Fixable Viability Kit (BioLegend; San Diego, CA) for 20 min at room temperature in phosphate buffer solution (pH 7.2S ...
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bioRxiv - Immunology 2024Quote: ... antibody or 100µL of PBS containing 0.2µg TruStain FcX™ and 0.04µL Zombie AquaTM Fixable Viability Kit (BioLegend cat#: 423102) and incubated at room temperature (RT ...
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bioRxiv - Immunology 2024Quote: ... mast cells were stained using CD117 (c-Kit)-APC or APC/Cy7 and FcεRIα-PE (all from Biolegend) at 1:200 dilution for 20 min on ice ...
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bioRxiv - Immunology 2024Quote: ... Release of IL-6 and IL-8 were measured using commercial ELISA kits (Biolegend, San Diego, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... centrifugated (500g) and IL-6 secretion analyzed using a commercial ELISA kit following the manufacturer’s instructions (Biolegend, #431304).
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bioRxiv - Immunology 2023Quote: ... The amount of secreted IFN-γ was determined the LEGEND MAX™ Human IFN-γ ELISA Kit (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Supernatant were collected and measured by ELISA kits according to the manufacturer’s instructions (catalog 438707, 440507, 431414; BioLegend). Absorbance was measured at 450 nm with a microplate reader (Molecular Devices).
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bioRxiv - Microbiology 2023Quote: ... Blood or tonsil CD4+ T cells were isolated using a human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were used without stimulation or co-stimulated with plate-bound CD3 and soluble CD28 antibodies in the presence of 20 ng/ml IL-2 for three days ...
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bioRxiv - Cancer Biology 2023Quote: ... untreated AML patients were determined by enzyme-linked immunosorbent assay (ELISA) using an IL21 Human ELISA kit (Biolegend), according to the manufacturer’s instructions.
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Fine tuning of CpG spatial distribution with DNA origami for improved therapeutic cancer vaccinationbioRxiv - Synthetic Biology 2023Quote: ... CD8 OT-I cells and CD4 OT-II were isolated by MojoSort kit purchased from BioLegend (#480035, #480033) according to the manufacturer protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were subjected to a live/dead staining with the Zombie NIR™ Fixable Viability Kit (423105; BioLegend) and incubated in the dark for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% BSA and 0.07% NaN3 in PBS) with additional Zombie Aqua™ (1:1,000 Fixable Viability Kit, BioLegend) to stain for vital cells ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected after 24h and extracellular IL-2 was measured using the Biolegend ELISAMax Deluxe Kit (Biolegend), according to manufacturer instructions.
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bioRxiv - Genomics 2023Quote: ... CD4+ T-cells were then isolated using the human CD4+ T-cell MojoSort negative magnetic selection kit (Biolegend). Enriched CD4+ T-cells were then rested overnight in a 37°C 5% CO2 incubator in complete RPMI (RPMI1640 ...
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bioRxiv - Immunology 2023Quote: ... both human and murine single cell suspensions were first stained with a viability marker (Fixable Viability Kit, Biolegend) according to manufacturer’s instructions to exclude dead cells ...
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bioRxiv - Neuroscience 2023Quote: ... Cell suspensions were first stained for viability using Zombie NIR™ Fixable Viability Kit (BioLegend, San Diego, CA) for 20 min at room temperature in PBS combined with FcR blocking antibody (1 μg ...
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bioRxiv - Molecular Biology 2023Quote: Cells were trypsinized and stained with Annexin V and 7-AAD using APC Annexin V staining kit (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Naïve B cells were isolated from spleens by immunomagnetic depletion using MojoSort Pan B cell Isolation Kit (Biolegend) and cultured in RPMI supplemented with L-glutamine ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 100 μL Zombie NIR (1:1000 in PBS, Zombie NIR™ Fixable Viability Kit, Biolegend, #423106), transferred into a 96-well plate and incubated for 20 min at 4 °C in the dark ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were subjected to staining using the Annexin V/Propidium Iodide (PI) apoptosis assay kit (BioLegend, USA) and subsequently analyzed using a BD FACSCalibur flow cytometer (USA) ...
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bioRxiv - Microbiology 2024Quote: ... IL-6 and TNF-α ELISA kits at proper dilutions according to the manufacturer’s instructions (BioLegend, CA, USA). For the detection of cytokines in serum and peritoneal fluid ...
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bioRxiv - Physiology 2024Quote: ... using the Pacific Blue Annexin V Apoptosis Detection Kit with 7-amino-actinomycin D (7-AAD) (BioLegend, #640926), according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: ... cells were washed once more with PBS and stained with the Zombie VioletTM Fixable Viability Kit (Biolegend, USA) for 15 min following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... and cell pellets were washed with PBS before being treated with the Zombie NIRTM Fixable Viability Kit (Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with PBS and stained for dead cells using Zombie UV Fixable Viability Kit (Biolegend, 423108) or Zombie NIR Fixable Viability Kit (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Single cell suspensions were stained with the Zombie NIR™ Fixable Viability KIT (780nm of excitation; Biolegend, #423106) at a 1/250 dilution in PBS (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: Detection of apoptotic cells was done by using FITC-Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend#640922). Briefly ...
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bioRxiv - Systems Biology 2019Quote: ... and IL-6 secreted protein level in cell supernatant from 1×106 human MoDM’s was performed by Legend Plex multi-analyte assay kit (BioLegend) according to manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... The cells were washed with 1xPBS and fixed using buffer of the True-Nuclear Transcription Factor Staining kit (Biolegend). After fixing ...
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bioRxiv - Immunology 2021Quote: ... IL-10 in human cell culture supernatants were measured using the LEGEND Max Human IL-10 ELISA kit (BioLegend). Technical duplicates were averaged and then normalized to the total number of live cells in each sample at the time of collection.
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bioRxiv - Immunology 2022Quote: ... Brefeldin A solution (420601) and CFSE Cell Division Tracker Kit (423801) were purchased from Biolegend (San Diego, CA, USA). PE anti-mouse IL-4 (12-7041-81) ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: Both FLT3L and GM-CSF BMDCs were stained with 1:1000 Zombie Green Fixable Viability Kit (BioLegend, Cat# 423111) and were blocked with anti-mouse CD16/32 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... The percentage of apoptotic cells were then determined using the FITC Annexin V apoptosis detection kit with PI (BioLegend) and flow cytometry as per manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from naïve gender- and age-matched WT and CD6Δd3 splenocytes by negative selection using magnetic beads (Mojo sort kit, BioLegend). Isolated cells were activated with 3 μg plate-bound anti-mouse CD3 (145-2C11 ...
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bioRxiv - Cancer Biology 2022Quote: ... and lineage-negative cells (Lin-) were analyzed for c-Kit (clone 2B8; eBioscience) and Sca-1 (clone D7; BioLegend) expression to discriminate Kit+ Sca-1- (KL ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were prepared for flow cytometry two days after media change using the FITC Annexin V kit (Biolegend, 640906). Quantification was performed using a BD FACSCanto 10 flow cytometer.
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bioRxiv - Immunology 2019Quote: TNF-α and IL-6 were measured in the media of activated BMDMs using ELISA kits purchased from Biolegend per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... cells were harvested and stained with AV and PI according to manufacturer’s manual (FITC Annexin V Apoptosis Detection Kit with PI; Biolegend). Stained cells were immediately analyzed by flow cytometry (MACSQuant ...
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bioRxiv - Cancer Biology 2021Quote: ... Apoptosis was determined by detecting phosphatidylserine using the APC-Annexin-V Apoptosis Detection Kit with PI (Biolegend, CA, USA). Briefly cells were harvested ...
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bioRxiv - Cancer Biology 2021Quote: Trypsinized and washed Panc02 cells were stained with the FITC Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend) and analyzed using the CytoflexS Flow Cytometer (Beckman Coulter).
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bioRxiv - Immunology 2021Quote: ... cells were fixed and permeabilized with a Transcription Factor Staining Kit (eBioscience) and stained using anti-IFNγ (XMG1.2; 200x; BioLegend) and recombinant mouse IL21R Fc Chimera (600x ...
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bioRxiv - Immunology 2021Quote: CD4+ T-cells were isolated from PBMC using the MojoSort™ Human CD4 T Cell Isolation Kit (480009; BioLegend) or by flow-cytometric sorting ...
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bioRxiv - Immunology 2021Quote: For differentiation of live and dead cells we stained cells with the Zombie Violet Fixable Viability Kit according to the manufacturer’s instructions (BioLegend). For surface marker analysis ...