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Citations for BioLegend :
5251 - 5300 of 5512 citations for Recombinant Human APCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... to assess viable cells and fluorophore-conjugated monoclonal antibodies to the following human cell-surface markers: anti-CD45-BV650 (Biolegend, 304044), anti-CD206-AF647 (Biolegend ...
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bioRxiv - Cell Biology 2023Quote: Lymph nodes were digested as described above and enriched for CD45- stromal cells by negative selection using MojoSort™ Human CD45 Nanobeads (Biolegend). CD45- cell enrichment was performed according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Cytokine levels in the supernatants from B cells that had been stimulated for 48 hr were measured using the LEGENDplex™ Human B cell Panel (13-plex) (Biolegend), using the same procedure as described for the antibody measurements ...
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bioRxiv - Immunology 2023Quote: Antibody levels in the supernatants from B cells that had been stimulated for 6 days were measured using the LEGENDplex™ Human Immunoglobulin Isotyping Panel (6-plex) (Biolegend), a bead-base immunoassay ...
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bioRxiv - Bioengineering 2023Quote: ... Successful blocking was confirmed by staining with anti-human CD44 antibody conjugated with Alexa Fluor 594 (clone: C44 Mab-5; Biolegend, USA) and quantified using the Cytek Aurora flow cytometer (4L 16V-14B-10YG-8R ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells were washed 3 times before adding 50 µL per well of Biotin anti-human IFN-γ Antibody (clone 4S.B3; #502504, BioLegend, USA) diluted 1:400 in 1% SureBlock solution and incubated 1 h at RT ...
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bioRxiv - Immunology 2023Quote: Cryopreserved PBMCs from blood collected 1 and 2 weeks after the H2-F boost were stained with anti-human monoclonal antibodies CD3 BV510 (OKT3, 1:400 dilution, BioLegend, RRID:AB_2561376), CD56 BV510 (HCD56 ...
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bioRxiv - Immunology 2023Quote: ... for 30 min at 4C in the dark before being incubated with Fc blocker (Human TruStain FcX, BioLegend, San Diego, California) in PBS with 1% BSA for 10 min at 4C ...
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bioRxiv - Cancer Biology 2023Quote: ... Phenotyping of AML patient samples used a 1:50 dilution of the PE-Cy7-conjugated anti-human CD34 antibody (clone 581, Biolegend, UK). Samples were stained ...
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bioRxiv - Cancer Biology 2023Quote: ... FFPE tissue sections of PDX tumours and their equivalent primary tumour region were subjected to hematoxylin and eosin (H&E) staining or immunohistochemistry using the following antibodies: anti-human CD45 (Clone: HI30; 1:200; BioLegend, #304002), anti-keratin (Clone ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then stained for transferrin receptor expression by adding 0.25 μg of PE/Cy7 anti-human CD71 [CY1G4] (Biolegend, Cat# 334111) directly to the blocking solution for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: The antibody mix for CTC detection of human CTCs in animal models contained a rat anti-mouse CD45 (30-F11) antibody conjugated to PE (Biolegend; 103106) and mouse anti-human HLA conjugated to AlexaFluor 647 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Cells were rinsed with FACS buffer (PBS containing 0.5% BSA and 2mM EDTA) for 3 min at 300g and incubated with Fc Receptor Blocking solution (Human TruStain FcX™, Biolegend) for 10 minutes prior to cell surface staining with fluorescently labeled antibodies in Brilliant Stain buffer (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: Ligand-independent reporter cell activation was assessed by overnight stimulation with 6.25 µg mL-1 Ultra-LEAF™ Purified anti-human CD3 (OKT3, BioLegend, #317326). Cells were incubated in antibody-coated wells and TCR responses were measured by flow cytometric analysis of GFP expression after 16h ...
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bioRxiv - Cancer Biology 2022Quote: ... LEPs from co-cultures were separated after 10 days for gene expression qPCR analysis by FACS using anti-human CD133-PE (BioLegend, clone7) and anti-human CD271-APC (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... K562 cells were settled with vehicle-treated or Verbenalin-treated NK cells in the presence of Brilliant Violet 421™ anti-human CD107a (LAMP1) antibody (Biolegend) and GolgiStop™ (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... for 30 min at 4C in the dark before being incubated with Fc blocker (Human TruStain FcX, Biolegend, San Diego, California) in PBS with 1% BSA for 10 min at 4C ...
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bioRxiv - Immunology 2023Quote: ... stained for 10 minutes at room temperature with Ghost Dye Violet 510 and Fc receptor blocking solution (Human TruStain FcX™, BioLegend), and washed once in FACS buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Remaining chemokine levels in the supernatants of cultured cells were measured in triplicate using the commercially available colorimetric LEGEND MAXTM human CCL5 ELISA kit (BioLegend, 440807) according to the manufacturer’s instructions and read with a SpectraMax M5 plate reader (Molecular Devices ...
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PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiationbioRxiv - Developmental Biology 2023Quote: The resultant single cells were stained with cocktails of antibodies diluted with FACS buffer listed as follows: PE-conjugated anti-mouse/human CD324 (E-Cadherin) antibody (1:500, Biolegend, 147303), PE/Cy7-conjugated anti-mouse CD117 (c-Kit ...
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bioRxiv - Immunology 2023Quote: ... buffer (PBS containing 2% FBS and 2 mM EDTA) and then incubated with human Fc receptor block (1:100 dilution, BioLegend, 422302) for 15 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with the following antibodies on ice for 30 minutes: Alexa Fluor 647 anti-human CD3 (1:100 dilution, BioLegend, 300321), Alexa Fluor 488 anti-human CD4 (1:200 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... cell suspensions were incubated for 20 min on ice with TotalSeq™-C anti-human Hashtag oligos (HTOs) (1:500, Biolegend, 394661 ...
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bioRxiv - Immunology 2023Quote: ... 1x106 B cells in 100 μl buffer were incubated with a panel of antibodies including 3 μl FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μl Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: The antibodies used for flow staining in this study are: rat anti-mouse/human CD11b conjugated to BV605 (clone M1/70, Biolegend, 101257), hamster anti-mouse CD3e conjugated to BV510 (clone 145-2C11 ...
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bioRxiv - Cancer Biology 2024Quote: Output of CD34+ cells following hematopoietic differentiation of hiPSCs was determined by staining with PE/Dazzle594-labeled anti-human CD34 antibodies (BioLegend, #343534). Prior to addition of antibodies ...
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bioRxiv - Bioengineering 2024Quote: ... Supernatants were collected on day 6 and immediately assessed for cytokine and chemokine secretion by LEGENDplex™ using LEGENDplex Human Inflammation Panel 1 (13-plex) with V-bottom Plate (Biolegend catalog #740809 ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were first incubated with 5uL of FcR blocker for 5 min at room temperature and stained with anti-CD45 antibody (BV785 anti-human CD45 antibody, BioLegend, 304048) and DAPI (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed twice with wash buffer and then incubated with FITC-conjugated anti-HA and PE-conjugated anti-human Fc (BioLegend, #342303) or PE-conjugated anti-Fab (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... The secretion levels of IL-1β and TGF-β were quantified using enzyme-linked immunosorbent assay (ELISA) by human IL-1β ELISA Max Deluxe kit (437004, BioLegend) and human TGF-β1 duoset ELISA kit (Dy204-05 ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were first incubated with 5uL of FcR blocker for 5 min at room temperature and stained with anti-CD45 antibody (BV785 anti-human CD45 antibody, BioLegend, 304048) and DAPI (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... These cells were stained with a cocktail of mAb conjugated with DNA-barcoded oligonucleotide that are specific for immune cell lineages (TotalSeq™-C Human TBNK Cocktail, BioLegend) according to the manufacturer’s protocol ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... washed cells were resuspended in 100 μL of FACS buffer with 5 μL of PE anti-human TMPRSS2 antibody (BioLegend #378403) per million cells and incubated for 30 min at 4 °C in the dark ...
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bioRxiv - Immunology 2024Quote: ... cell pellets resuspended in 100 μl FACS buffer (phosphate buffer saline containing 1% BSA and 0.1% sodium azide) were treated with human TruStain FcX™ (Biolegend #422302) for 15 min on ice followed by incubation with rabbit anti-human LBR antibody (Abcam #32535 ...
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bioRxiv - Immunology 2019Quote: ... then incubated with antibodies specific for intracellular proteins and Brilliant Violet 605-streptavidin conjugate (BioLegend) to detect biotin-conjugated antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... The proteins were analyzed by SDS-PAGE and immunoblotting with anti-GST (BioLegend MMS-112P) and anti-His (Qiagen 34660 ...
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bioRxiv - Immunology 2020Quote: Interferon beta (IFNβ) protein levels in plasma were measured using Legendplex Mouse Inflammation Panel (Biolegend).
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bioRxiv - Immunology 2021Quote: The following antibodies have been used to stain extracellular proteins: anti-CD8-Alexa488 (Biolegend 100723), anti-PD1-PE (eBioscience 12-9981-83) ...
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bioRxiv - Microbiology 2019Quote: ... The following antibodies were used for detection of proteins of interest: HA.11 (mouse, Biolegend), β-actin (mouse ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were analyzed by SDS-PAGE and immunoblotting with anti-GST (BioLegend MMS-112P) and anti-His (Qiagen 34660 ...
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Co-stimulatory molecules decide T cell fate through regulations of their invigoration and impairmentbioRxiv - Molecular Biology 2022Quote: Synchronized EL4 cells were stimulated by Ig Protein with anti-CD3 (final 2μg/ml, Biolegend) conjugated magnetic beads ...
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bioRxiv - Immunology 2022Quote: ... endogenous biotin and avidin proteins were successively blocked using an Avidin/Biotin Blocking Kit (Biolegend), for 10 min each ...
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bioRxiv - Neuroscience 2022Quote: ... The following antibodies were utilized for immunoblotting: myelin basic protein (MBP; Biolegend; 808401; 1:1000), PLP (rat hybridoma ...
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bioRxiv - Immunology 2024Quote: ... and FcεRIγ (FCABS400F antibody, Milli-pore)10 protein expression on live (Zombie red, 77475; BioLegend), CD3-negative (300318 antibody ...
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bioRxiv - Immunology 2024Quote: The following antibodies have been used to stain extracellular proteins: BV711 anti-CD45RA (Biolegend 304138), APC anti-CD45RO (Biolegend 304210) ...
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bioRxiv - Cell Biology 2024Quote: Flow cytometry and ImageStream reagents: Antibodies against the following proteins were used: CD41 (MWReg30, Biolegend), Ter119 (Ter119 ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration in the supernatant was measured by Bradford assay (BioLegend, San Diego, CA, USA). Proteins (10-40 μg ...
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bioRxiv - Immunology 2021Quote: ... the supernatants were collected to determine the levels of antibody productions (e.g., IgG1, IgG2, IgG3, IgG4, IgA, and IgM) by using LEGENDplex™ Human Immunoglobulin Isotyping Panel (Biolegend, CA, USA). The Gallios Flow Cytometer was utilized to analyze the data according to the manufacturer’s recommended protocol.
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bioRxiv - Immunology 2021Quote: Fluorophore- or biotin-conjugated antibodies specific for human cell-surface antigens and cytokines were as follows: anti-CD71 (CY1G4, BioLegend, DF1513, NovusBio), anti-CD235a (HI264 ...
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bioRxiv - Microbiology 2022Quote: ... Quantification of cytokine and chemokine levels in culture media was performed using the LEGENDPlex kit from BioLegend (human anti-virus response panel), according to the manufacturer’s recommendations.