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Citations for BioLegend :
5051 - 5100 of 6089 citations for Piggybac Transposable Element Derived 3 PGBD3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... total lung tissue was marked with a lineage antibody (Lin, anti-mouse CD3ε, Ly-6G / Ly-6C, CD11b, CD45R / B220, TER-119 PE, Biolegend), anti-EpCAM for 10 minutes at temperature environment ...
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bioRxiv - Immunology 2022Quote: ... total lung tissue was marked with a lineage antibody (Lin, anti-mouse CD3ε, Ly-6G / Ly-6C, CD11b, CD45R / B220, TER-119 PE, Biolegend), anti-CD45 (BD Bioscience ...
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bioRxiv - Immunology 2020Quote: ... For baseline T cell frequencies 0.5e6 cells from baseline uninfected total lymphocyte samples were stained and analyzed as above with phenotype antibody panel as follows: CD95-APC (2μl, Biolegend 305611), CCR7-PE (2μl ...
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bioRxiv - Genomics 2020Quote: ... The remaining 450μl of cell suspension was stained with 15 μl of each of the following antibodies: CD3 APC (Biolegend, 300411), CD4+ FITC (BD ...
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bioRxiv - Immunology 2021Quote: Cells were stained with the following surface antibodies: Anti-human CD8a FITC (1:100, clone RPA-T8, BioLegend, cat # 301050), Anti-human TCRgd FITC (1:100 ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... activated T-cells were treated with HDACI as described before and after 72 h total protein acetylation of the cells was determined by flow cytometry using PE-coupled anti-Acetylated Lysine Antibody (Biolegend). Cells were washed with PBS ...
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bioRxiv - Immunology 2021Quote: ... The resultant cell suspension was stained with the following antibodies directed against cell surface antigens for 30 minutes at 4°C: CD45::BV421 (H130, BioLegend) and HLA-DR::PE-Cy7 (L243 ...
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bioRxiv - Genomics 2020Quote: ... isolated single cells were co-stained in FACS buffer with FITC-conjugated mouse anti-human CD45 antibody (1:25; clone HI30, Biolegend), PE-conjugated mouse anti-human CD31 antibody (1:25 ...
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bioRxiv - Immunology 2021Quote: ... cell-free supernatants were collected followed by ELISA to detect IL-10 and TGFβ using capture and detecting antibodies (Biolegend). Other M2 phenotype characteristics including the cell surface expressions of CD206 ...
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bioRxiv - Immunology 2021Quote: ... single-cell suspensions were obtained and cells were stained with the following monoclonal antibodies: CD45 (APC-Cy7 #103116 1/200), CD11B (APC #101212, 1/200), Ly6G (PE-Cy7 #127618, 1/100) from Biolegend, Siglec-F (PE #552126 ...
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bioRxiv - Immunology 2021Quote: FACS purified cell subsets were labeled by incubation for 30 minutes at 4°C with TotalSeq anti-mouse Hashtag antibodies (BioLegend), then washed ...
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bioRxiv - Immunology 2021Quote: ... TCR expression was analyzed the next day by flow cytometry using an anti-mouse TCR antibody (H57-597, BV421, BioLegend).
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bioRxiv - Immunology 2021Quote: ... DC purity was assessed by antibodies against CD141 (1:10, APC, AD5-14H12, 130-113-314, Miltenyi) and XCR1 (1:10, PE, S15046E, 372604, BioLegend), and T cell phenotype was assessed by antibodies against CD25 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... the biological activity of TNFα was neutralized with an anti-TNFα antibody (Clone MP6-XT22) (BioLegend, San Diego, CA, USA). Cultures with isotype-matched antibody (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... these cells were washed and incubated with the secondary antibody conjugated with Alexa Fluor 488 (Biolegend, San Diego, CA, USA) (1:400 ...
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bioRxiv - Systems Biology 2021Quote: ... A maximum of 1×106 cells/donor were stained by oligo-barcoded antibodies (TotalSeq-A or TotalSeq-C; Biolegend, Expedeon) for 30 min on ice ...
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bioRxiv - Neuroscience 2020Quote: Free floating 30 μm sagittal brain sections were treated for antigen retrieval using sodium citrate buffer and subsequently stained using primary antibodies for Iba1 (1:250, rabbit, Wako) and anti-6E10 (1:1000, Biolegend), and the corresponding secondary antibodies and nuclear counterstain using DAPI ...
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bioRxiv - Neuroscience 2020Quote: ... samples have been incubated overnight at 4°C with primary antibody anti β-amyloid (Aβ) (clone 6E10, 1:500, BioLegend) diluted in blocking buffer and stained with the corresponding goat secondary antibody ...
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bioRxiv - Neuroscience 2021Quote: The following antibodies were used at the indicated concentrations (IHC-immunohistochemistry; ICC-immunocytochemistry; IB-immunoblot): purified anti-HA mouse (Biolegend Cat# 901501 ...
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bioRxiv - Bioengineering 2021Quote: ... Bcl-2 anti-apoptotic protein expression was measured by intracellular staining of BMMs with PE-Cy7 anti-Bcl-2 antibody (Biolegend). AlexaFluor488 anti-Bcl-2 and BV711 anti-CD11b antibodies (Biolegend ...
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bioRxiv - Bioengineering 2020Quote: The following antibodies were used for phenotyping and proliferation assays: anti-CD3-PE/Cy7 (clone SK7, Biolegend, San Diego, CA), anti-CD4-PerCP (clone SK3 ...
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bioRxiv - Bioengineering 2020Quote: ... Staining buffer used was a 1:1000 dilution of stock antibody solution (APC anti-mouse CD45; Alexa Fluor 488 anti-mouse Ly6G, Biolegend) into FACS buffer ...
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bioRxiv - Biochemistry 2021Quote: ... with remaining cells washed and resuspended in 1x PBS with 1% BSA and stained for 15 minutes with the following antibodies: human CD45-APC (Clone 2D1) (Biolegend), human CD33-PE-Cy7 (Clone P67.6 ...
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bioRxiv - Neuroscience 2021Quote: ... Single Cell suspensions were incubated with an anti-FcγIII/II receptor antibody (clone 93, eBioscience) to block unspecific binding and Zombie NIR™ (Biolegend), a fixable viability dye ...
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bioRxiv - Neuroscience 2021Quote: ... CD8+ T cells and CD45/CD11b+ cells (macrophages/microglia) was performed using the following antibodies in a 1:200 dilution: anti-CD3e (clone 145-2C11, BioLegend), anti-CD4 (clone GK 1.5 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with 1.5% normal serum followed by incubation with the primary antibody (biotinylated anti-Aβ – 1:100, BioLegend, USA) for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... All FACS antibodies were used at 1:100 dilution in the presence of TruStain fcX blocker (101320, Biolegend, 1:50). For PDGFRa immunofluorescence staining ...
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bioRxiv - Genomics 2021Quote: ... and then up to 500,000 cells from each condition were stained with a unique TotalSeq™-A anti-human Hashtag antibody (BioLegend) in 50 ul for 30 minutes at 4°C on a Laminar Wash™ 16-well strip (Curiox Biosystems) ...
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bioRxiv - Bioengineering 2020Quote: ... cells were first incubated in a cocktail of PE anti-mouse CD169 and PE anti-mouse F4/80 antibodies (BioLegend). After washing ...
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bioRxiv - Cancer Biology 2020Quote: ... The purity of isolated MDSCs was checked by flow cytometry using Gr1 FITC and CD11b APC antibodies (purchased from Biolegend). MDSCs were grown in exosomes depleted media consisting of RPMI ...
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bioRxiv - Cell Biology 2021Quote: ... Ly6G-PE-Cy7+ and Ly6G- APC-) and intravascular PMNs (CD45-PerCP-Cy5·5+; Ly6G-PE-Cy7+ and Ly6G-APC+) (all antibodies from BioLegend) by flow cytometry (FACS Canto II ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C in primary antibodies diluted in 1-5% serum in PBS-T according to the following concentrations: novel Parkin mAbs from Biolegend clones D (BioLegend ...
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bioRxiv - Bioengineering 2021Quote: ... Naïve T cell marker staining was performed with APC/Cy7 mouse anti-human CD45RA (#304128) and BV510 mouse anti-human CD45RO (#304232) antibodies from Biolegend.
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bioRxiv - Cell Biology 2021Quote: ... Tissue sections were then washed and stained with an AF647-directly conjugated antibody towards CCR6 (1:200, clone G034E3, BioLegend) at 37 ° C for one hour ...
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bioRxiv - Systems Biology 2020Quote: ... The cells were incubated with each antibody for 45 min at −22–25°C after FcR blocking using Fc blocker (Human TruStain FcX, Biolegend) or anti-mouse CD16/CD32 antibody ...
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bioRxiv - Systems Biology 2020Quote: ... cells were stained on ice for 30 min with the following fluorochrome-conjugated antibodies in a 12-color staining combination: APC anti-IL17RA (clone W15177A, BIolegend) at 1:50 ...
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bioRxiv - Cell Biology 2021Quote: ... Platelet activation was detected by direct immunofluorescence labeling of alpha granule release marker CD62P (P-selectin) with mouse anti-human monoclonal antibody CD62P-AlexaFluor647 (Clone AK4, Cat.No. 304918, BioLegend, USA). and activation associated conformational change in integrin αIIbβ3 was detected with a mouse anti-human monoclonal antibody PAC1-FITC (Clone PAC-1 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were labeled with the following antibodies directly conjugated to the following fluorophores: CD45-PE/Cy7 (clone 30-F11, BioLegend), CD11b-BV510 (clone M1/70 ...
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bioRxiv - Genetics 2020Quote: ... and stained at 4°C in a final volume of 50µL with the following antibodies: CD14-FITC (1:50)(Biolegend 301803), CD16-PE (1:50)(Biolegend 3G8) ...
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bioRxiv - Immunology 2021Quote: ... Intracellular staining using antibodies to detect IRF4 (3E4) and PRDM1 (5E7) was performed using True-Nuclear Transcription Factor Buffer Set (Biolegend). For mitochondrial stains ...
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bioRxiv - Immunology 2021Quote: ... 0.05 mM β-mercaptoethanol) on a 96-well flat bottom plate coated with anti-CD3 antibody (10µg/ml, cat. # 317325, BioLegend). 7 x 103 DC3 or 5 x 104 monocytes were used per well ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were harvested using StemPro™ Accutase™ cell dissociation reagent and incubated with antibodies against CD13 (PE, 301704, Biolegend), SSEA4 (AF647 ...
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bioRxiv - Molecular Biology 2021Quote: ... while the MDR1 replicons were stained with 5 μL of PE anti-human CD243 (ABCB1) antibody clone 4E3.16 (Biolegend, US). After staining for 30 min on ice ...
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bioRxiv - Microbiology 2021Quote: ... and the membranes were probed with a rabbit polyclonal antibody generated against the FHA mature C-terminal domain (MCD)(10) and a mouse monoclonal antibody generated against an HA epitope (BioLegend). Corresponding α-rabbit and α-mouse IRDye secondary antibodies (LI-COR Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed with water three times before applying 25 μL of secondary antibody (goat anti-mouse IgG Fc, BioLegend) at a dilution of 1:4,000 in blocking solution ...
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bioRxiv - Biophysics 2020Quote: ... Nup107 in HeLa Kyoto mEGFP-Nup107 cells were labelled with an Alexa Fluor 647 anti-GFP rat monoclonal primary antibody (Clone FM264G, BioLegend) for dSTORM imaging (Figure 4a ...
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bioRxiv - Cancer Biology 2020Quote: ... All other antibodies used for immune fluorescence and flow cytometric analysis and human IL-1beta ELISA kit were acquired from Biolegend. Human was obtained from In vitro experiments were conducted using complete culture media consisting of RPMI 1640 medium supplemented with 20 mM HEPES ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell sorting for GFP-positive CD298+MHC-I− PDX cells with fluorescently tagged antibodies for CD298 (Biolegend, cat. no. 341704) and MHC-I (eBioscience ...
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bioRxiv - Cancer Biology 2020Quote: ... The homogenates were then resuspended in PBS buffer and stained with anti-mouse CD45.1 antibody conjugated with Brilliant Violet 421 (clone A20; BioLegend, 103134), CD11b monoclonal antibody conjugated with APC (M1/70 ...
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bioRxiv - Cell Biology 2021Quote: ... Mononuclear cells were incubated with APC (allophycocyanin) or PE (phycoerythrin)-conjugated anti-mouse Ly-6A/E (Sca-1) antibody (#122508, BioLegend), APC or PE-conjugated anti-mouse CD45 antibody (#103106 ...