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Citations for BioLegend :
5001 - 5050 of 6089 citations for Piggybac Transposable Element Derived 3 PGBD3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... supplemented with recombinant IL-2 (R&D Systemts) at 10ng/mL and 4µg/mL of anti-CD28 antibody (Biolegend), plated in 24-well plates coated with 2µg/mL of anti-CD3 antibody (Biolegend) ...
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bioRxiv - Neuroscience 2024Quote: ... isolated cells were stained with the following antibodies: CD11b-FITC (1:200 dilution, cat# 101205, clone M1/70, BioLegend), ACSA-2-PE (1:200 dilution ...
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bioRxiv - Immunology 2024Quote: ... followed by another wash with PBS and staining with fluorescently conjugated monoclonal antibodies against APC conjugated anti-human CD56 (clone 5.1H11, Biolegend) for 20 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Pluripotency marker expression was assessed every 10 passages by flow cytometry (PE anti-human SSEA-4 antibody, BioLegend, #330405) or RT-qPCR for DNMT3B ...
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bioRxiv - Neuroscience 2024Quote: ... followed by an overnight incubation with the second set of primary antibodies [1/100 anti-CD9-biotin (312112, Biolegend) and anti-P2RY12)] ...
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bioRxiv - Microbiology 2024Quote: ... and PerCP-Cy5.5-conjugated (non-Mtb experiments) or APC-conjugated (Mtb experiments) anti-HLA-DR antibody (BioLegend, clone L243) for 20 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... followed by incubation with TruStain FcX™ PLUS (anti-mouse CD16/32) Antibody (Biolegend, cat#156603; 0.25 µg/sample) for 10 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... followed by incubation with TruStain FcX™ PLUS (anti-mouse CD16/32) Antibody (Biolegend, cat#156603; 0.25 µg/sample) for 10 min ...
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bioRxiv - Bioengineering 2024Quote: ... The bound TCE were then detected via flow cytometry using an anti-His-tag APC antibody (cat. # 362605, BioLegend).
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bioRxiv - Biochemistry 2024Quote: ... to remove dead cells and then labeled with the following antibodies to enable downstream macrophage sorting: CD45 (#103107 Biolegend), CD11b (#740861 BD-Biosciences) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained with the following anti-mouse antibodies: APC/Fire 810 CD3 (17A2, catalog no. 100267, Biolegend), CD11b BV570 (M1/70 ...
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bioRxiv - Bioengineering 2024Quote: ... samples were washed twice with flow buffer (HBSS with 0.25% BSA) and stained with an Alexa Fluor 647-conjugated anti-human CD8 antibody (BioLegend) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: The T cells generated under standard conditions were stained on day 5 of culture with the antibodies Thy1.1-APC (Biolegend) and pan-TCR-α/β-PE (Beckman Coulter) ...
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bioRxiv - Cancer Biology 2024Quote: ... and labeled with anti-MHC-I fluorescent antibody (anti-mouse MHCH-2kd/H-2Dd, BioLegend, Clone: 34-1-25). Cells were then washed with PBS and analyzed with the NovoCyte Advanteon Flow Cytometer (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... each well was coated with 70 µL of a 1:500 dilution of a biotinylated 6E10 antibody (#803008, BioLegend) in 0.05% Tween-20 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were stained with Pacific Blue™ (PB)-conjugated anti-CD45 antibody (#103126, BioLegend, CA, US; dilution 1:200), PB-conjugated anti-CD31 antibody (#102422 ...
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bioRxiv - Bioengineering 2024Quote: ... streptavidin-conjugated MBs were washed once by centrifugation and incubated with 6 µg biotin anti-human αvβ3 antibody (304412, BioLegend) on ice for 30 min ...
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bioRxiv - Bioengineering 2024Quote: Jurkat cell activation was induced using Human (0.5 µg/ml) T-Activator anti-CD3 and anti-CD28 antibodies (BioLegend) for cell expansion and activation.
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bioRxiv - Bioengineering 2024Quote: ... CD8+ T cells were stained overnight at 4°C using Alexa Fluor 647-conjugated anti-human CD8 antibody (BioLegend). Confocal imaging was performed using an inverted laser scanning confocal microscope ...
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bioRxiv - Cancer Biology 2024Quote: ... The expression of immune checkpoint receptors was determined using the following antibodies: PD-1 PE-Dazzle 594 (329940, Biolegend), VISTA BV421 (566750 ...
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bioRxiv - Cancer Biology 2024Quote: ... appropriate concentrations of specific antibodies were added for 30C:min at 4°C with 1µg/ml anti-CD31-AlexaFluor 488 (Biolegend, 303110), 1:800 anti-CD45-BUV805 (BD Biosciences) ...
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bioRxiv - Cancer Biology 2024Quote: ... The complexes formed by the beads-bound EVs were then stained with anti-CD81 APC antibody (Biolegend, clone 5A6) and signal was detected using Cytoflex flow cytometry (Beckman Coulter) ...
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bioRxiv - Cancer Biology 2021Quote: ... diluted at 1X concentration for 30 min on ice with a master mix of a combination of primary antibodies (E-cadherin PE/Cy7 Biolegend, Clone 67A4 ...
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bioRxiv - Cancer Biology 2021Quote: ... diluted at 1X concentration for 30 min on ice with a master mix of a combination of primary antibodies (E-cadherin PE/Cy7 Biolegend, Clone 67A4, 324 115; CD10 BV421, BioLegend, Clone HI10a ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then stained in FC buffer (2% FCS in PBS) with anti-CD3-APC conjugated antibodies (1:80; BioLegend) and LIVE/DEAD Fixable Near-IR Dead Cell Stain (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were stained at the same time with the APC conjugated surface marker antibody CD45 after a 5 min blocking step with Human TruStain FcX solution (20x, BioLegend) diluted in 1 % BSA/PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Primary NK cells from lineage-depleted apheresis or NK92 cells were incubated on cells or cell-free matrix for 30 minutes in the presence of directly conjugated antibodies to surface antigens CD56 (1:50, clone HCD56, Biolegend) and CD29 (1:50 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell suspensions were incubated with the peptides for 1 hour following fixation with formaldehyde and staining with cytochrome c antibody (BioLegend, Alexa Fluor® 647 anti-Cytochrome c - 6H2.B4 ...
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bioRxiv - Immunology 2021Quote: ... cells were subsequently blocked in flow buffer supplemented with either anti-mouse CD16/CD32 antibody (eBioscience) or Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: ... flow buffer was supplemented either with anti-mouse CD16/CD32 antibody (eBioscience) or Human TruStain FcX Fc Receptor Blocking Solution (BioLegend). The following antibodies were used for flow analysis ...
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bioRxiv - Cell Biology 2020Quote: ... Unlabeled cells were then centrifuged at 300 x g and labeled with a cocktail of 1 μg/ml of PerCP-Cy5.5 anti-EPCAM antibody and Zombie Aqua viability stain (Biolegend 423101) diluted 1:400 from stock concentration in FACS buffer.
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bioRxiv - Cell Biology 2020Quote: ... followed by incubation on ice with labeling cocktail consisting of 1 μg/ml of PerCP-Cy5.5 anti-EPCAM antibody and Zombie Aqua viability stain (Biolegend 423101) diluted 1:400 from stock concentration in FACS buffer ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: Cells were incubated with anti-CD16/32 (Fc block) prior to staining with the following fluorophore or biotin conjugated monoclonal antibodies purchased from Biolegend, eBioscience ...
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bioRxiv - Immunology 2021Quote: PBMCs were stained with the following antibodies and proteins for flow sorting: anti-CD19-APC (1:10 dilution, clone HIB19, cat. no. 982406, BioLegend), anti-IgD-FITC (1:20 dilution ...
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bioRxiv - Immunology 2021Quote: ... Eluted fractions were pooled and purified proteins were verified for integrity by probing with an anti-HIS tag antibody (Biolegend) as well as with subtype-specific mAbs via SDS-PAGE and Western blot.
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bioRxiv - Immunology 2021Quote: ... the cell suspensions were stained with the following antibodies: PE anti-mouse Ly-6C (BioLegend, 128008, clone HK1.4, 1:200), PerCP/Cyanine5.5 anti-mouse/human CD11b (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained with the following antibodies and stains (in multiple sets to prevent fluorophore overlap): CD4-FITC (BioLegend), CD8-PECy5 (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... total thymocytes were processed to single cell suspension in 10% FBS/HBSS (20×106 cells/ml) and incubated with rat anti-mouse CD24 antibodies (M1/69, Biolegend) (30 μg/100 ×106 cells ...
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bioRxiv - Immunology 2021Quote: ... containing the following fluorescently-labelled antibodies diluted in FACS buffer (2% FBS in PBS): CD4-APC-Cy7 (clone A161A1; BioLegend), CD8a-Alexa700 (clone SK1 ...
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bioRxiv - Immunology 2021Quote: ... Antibodies for flow cytometry and cell isolation against the following proteins (all mouse unless otherwise indicated) were obtained from BioLegend, eBioscience ...
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bioRxiv - Immunology 2021Quote: ... cells were washed twice in FACS buffer and incubated with rat anti-human Fc antibody conjugated to allophycocyanin (Cat: 410712, BioLegend) for 15 minutes on ice ...
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bioRxiv - Immunology 2021Quote: 1-2 × 106 fresh decidual leukocytes were washed with PBS and stained using the following cocktail of antibodies: CD45 (HI30, Biolegend), CD66b (G10F5 ...
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bioRxiv - Immunology 2021Quote: ... The sorted fraction was sequentially stained for 20 min at 4 °C with the Ly51-PE antibody (1:800) (Biolegend) and depleted of Ly51-positive cells using anti-PE Microbeads (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2022Quote: Cells were washed with PBS and stained with the following panel of antibodies to quantify and enrich for LT-HSCs: anti-CD34-PerCP-Cy5.5 (Biolegend, 343612), anti-CD45RA-APC-H7 (BD ...
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bioRxiv - Microbiology 2022Quote: ... For baseline T cell frequencies 0.5(10)6 cells from baseline uninfected total lymphocyte samples were stained and analyzed as above with phenotype antibody panel as follows: CD95-APC (2μl, Biolegend 305611), CCR7-PE (2μl ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated at 4□ overnight with the following primary antibodies: mouse anti-C3 (1:200 dilution; Biolegend cat. no. 846302), chicken anti-GFAP (1:300 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubation (VMAT2, 1:50, EB06558, Everest Biotech, Ramona, USA, HA-tag: 1:50, 682404, Biolegend, San Diego, USA) was performed for 60 min ...
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bioRxiv - Molecular Biology 2020Quote: ... and c-Kit on mast cells were analyzed by Flow cytometry using fluorochrome labelled antibodies (PE-CY7–conjugated anti-FcεRIα, MAR-1 Biolegend #134317; allophycocyanin-CY7–conjugated anti–c-Kit, 2B8, Biolegend, #105825). The YFP+ cells were FACS-sorted and deletion of the Gata2 gene was determined by PCR to be greater than 99%.
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bioRxiv - Cell Biology 2022Quote: ... 105 - 106 cells were resuspended in FACS buffer with 1 µg/ml TruStain FcXTM anti-mouse CD16/32 antibody (BioLegend) to block for 5 minutes before adding conjugated surface antibodies in FACS buffer at a 2X concentration ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were enriched via negative selection using biotinylated antibodies at the concentration indicated in Table 1 and using MojoSort Streptavidin Nanobeads (BioLegend). Enrichments were completed in 1X HBSS (Ca2+ and Mg2+ free ...